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The map label for this gene is azoR4 [H]

Identifier: 157372400

GI number: 157372400

Start: 4621797

End: 4622423

Strand: Direct

Name: azoR4 [H]

Synonym: Spro_4167

Alternate gene names: 157372400

Gene position: 4621797-4622423 (Clockwise)

Preceding gene: 157372390

Following gene: 157372418

Centisome position: 84.82

GC content: 45.93

Gene sequence:

>627_bases
ATGTTAACCAGAGAATATTTAATGAAAAACGTATTGATTTTGAAATCAAGCATCAATGGAGATGCGTCGCTAACAAACCA
ATTGATAGATGAATTTAAATCGCAGCGGCAGCAGGCAGGGCACGATGAAAGGATCATTGAACGTGACCTTACTACTCTCG
CTCTACCGAAACTTAGTCACTCACTTTTTGCCGCCATGCGTGGCGGAGATATCTCAGATCCTTCTATTAAAGAGGGGGTT
GCGTTATCGGATACGCTGATAAATGAATTAAAATCAAGCGATTTACTGGTCATCGGTGCACCAATGTATAACCTGAATGT
TCCGACAGATTTAAAAAAGTGGTTCGACCTGGTGACTCGTGCCAAAGAAACTTTCCGTTATACCGAAACCTACCCGCAAG
GGCTGGTAGAGCACGTCAGGGCTGTGGTGATTAGTTCCCGCGGCGGTGTGCATGAAGGGCAATCAACCGATGCTGTAACC
CCATATCTACGAGCTGTTTTGGGGTTAATGGGGATCATCGATGTCGATTTCATCTATGCAGAAGGAATCGATATCAAACC
ATACGGTCGCGATACTGGTATAGCCCATGCCCAAGAGCAAATAGCCCGCGCCGCACATCTGGCTTGA

Upstream 100 bases:

>100_bases
TTAAGATCGGTACGCATCAATTGGTTCCATTATGTATACATTCTCTATACACAATGTCACTAATGGAGAAAGAAACATAG
TGATATTCTATGGGTAATGA

Downstream 100 bases:

>100_bases
TAGCCAGCTGATTATGTATTCTGGTCTGTTATCCATTTAGTTTTAATAAGTAAAAATTAAAAAAAATCAGTAACCTATAA
TACTTTCAAAGCAGGACTGC

Product: NAD(P)H dehydrogenase (quinone)

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 4; FMN-dependent NADH-azo compound oxidoreductase 4 [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MLTREYLMKNVLILKSSINGDASLTNQLIDEFKSQRQQAGHDERIIERDLTTLALPKLSHSLFAAMRGGDISDPSIKEGV
ALSDTLINELKSSDLLVIGAPMYNLNVPTDLKKWFDLVTRAKETFRYTETYPQGLVEHVRAVVISSRGGVHEGQSTDAVT
PYLRAVLGLMGIIDVDFIYAEGIDIKPYGRDTGIAHAQEQIARAAHLA

Sequences:

>Translated_208_residues
MLTREYLMKNVLILKSSINGDASLTNQLIDEFKSQRQQAGHDERIIERDLTTLALPKLSHSLFAAMRGGDISDPSIKEGV
ALSDTLINELKSSDLLVIGAPMYNLNVPTDLKKWFDLVTRAKETFRYTETYPQGLVEHVRAVVISSRGGVHEGQSTDAVT
PYLRAVLGLMGIIDVDFIYAEGIDIKPYGRDTGIAHAQEQIARAAHLA
>Mature_208_residues
MLTREYLMKNVLILKSSINGDASLTNQLIDEFKSQRQQAGHDERIIERDLTTLALPKLSHSLFAAMRGGDISDPSIKEGV
ALSDTLINELKSSDLLVIGAPMYNLNVPTDLKKWFDLVTRAKETFRYTETYPQGLVEHVRAVVISSRGGVHEGQSTDAVT
PYLRAVLGLMGIIDVDFIYAEGIDIKPYGRDTGIAHAQEQIARAAHLA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=181, Percent_Identity=50.2762430939227, Blast_Score=171, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 22980; Mature: 22980

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTREYLMKNVLILKSSINGDASLTNQLIDEFKSQRQQAGHDERIIERDLTTLALPKLSH
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLFAAMRGGDISDPSIKEGVALSDTLINELKSSDLLVIGAPMYNLNVPTDLKKWFDLVTR
HHHHHHCCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEECCEEECCCCHHHHHHHHHHHH
AKETFRYTETYPQGLVEHVRAVVISSRGGVHEGQSTDAVTPYLRAVLGLMGIIDVDFIYA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEE
EGIDIKPYGRDTGIAHAQEQIARAAHLA
CCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTREYLMKNVLILKSSINGDASLTNQLIDEFKSQRQQAGHDERIIERDLTTLALPKLSH
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLFAAMRGGDISDPSIKEGVALSDTLINELKSSDLLVIGAPMYNLNVPTDLKKWFDLVTR
HHHHHHCCCCCCCCCHHCCCHHHHHHHHHHCCCCEEEEECCEEECCCCHHHHHHHHHHHH
AKETFRYTETYPQGLVEHVRAVVISSRGGVHEGQSTDAVTPYLRAVLGLMGIIDVDFIYA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEE
EGIDIKPYGRDTGIAHAQEQIARAAHLA
CCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA