Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 157372259

GI number: 157372259

Start: 4467901

End: 4468632

Strand: Direct

Name: rsmE [H]

Synonym: Spro_4025

Alternate gene names: 157372259

Gene position: 4467901-4468632 (Clockwise)

Preceding gene: 157372258

Following gene: 157372260

Centisome position: 82.0

GC content: 57.51

Gene sequence:

>732_bases
ATGCGCATACCTCGCATTTATCACCCGCAGCCGTTAATCGACCATGCGGAAATCTTCTTAAGCGAAGACGCCGCCAACCA
TATCGGGCGTGTACTGCGCATGAACGCCGGCCAGGCGCTGCAGTTATTCGACGGCAGCAATCAGGTGTTTGACGCCGTGA
TCGCCCAGGTCGACAAGAAAAGCGTGCAAGTGCAGCTCAGCGCCGGCCAGTTGGAAGACCGTGAATCACCGCTCAATCTG
CATCTGGGTCAGGTAATTTCTCGCGGTGAGAAAATGGAGTTCACCATTCAGAAATCCATCGAGCTGGGGGTCAACGTCAT
TACCCCGCTGTTTTCCGAGCGCTGCGGCGTTGCGCTTTCCGGCGAACGCCTGGCGAAAAAAATTCAGCAGTGGCAGAAGA
TTGCCATCGCCGCCTGCGAACAGTGCGGCCGCAACCGCATCCCGGAAATCCGCGAGGCCATGCAGCTGGAAGCCTGGTGT
GCCGAGCAGGATGGCAGCCTCAAGCTTAACCTGCACCCGCGCGCCAGCCATAGCATCAACACCCTGCCGCTACCGGTGGA
AAATGTCCGGTTGCTGATTGGGCCGGAAGGCGGTTTATCTACAGGCGAAATTGCGATGACCACAGGCCACGGATTTACTG
ATATCCTGCTGGGGCCACGCGTTCTGCGTACAGAAACCACAGCGCTCACCGCAATTACCGCTTTGCAGGTACGTTTCGGC
GATCTGGGTTAA

Upstream 100 bases:

>100_bases
ACTTGCCGCCTTGCTGCAGCTTCAATTACTTTGGGTATATACTCTTTTTTTTCAAGCTGCAGCACTGATGACTGCACATG
AGTCAGGATCGAATATTCAT

Downstream 100 bases:

>100_bases
AGGAGAAAAGATGATTAAGCTCGGCATCGTGATGGACCCTATCGCCTCCATCAATATCAAGAAAGACACCAGCTTCGCCA
TGCTGCTTGAAGCGCAGCGC

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIYHPQPLIDHAEIFLSEDAANHIGRVLRMNAGQALQLFDGSNQVFDAVIAQVDKKSVQVQLSAGQLEDRESPLNL
HLGQVISRGEKMEFTIQKSIELGVNVITPLFSERCGVALSGERLAKKIQQWQKIAIAACEQCGRNRIPEIREAMQLEAWC
AEQDGSLKLNLHPRASHSINTLPLPVENVRLLIGPEGGLSTGEIAMTTGHGFTDILLGPRVLRTETTALTAITALQVRFG
DLG

Sequences:

>Translated_243_residues
MRIPRIYHPQPLIDHAEIFLSEDAANHIGRVLRMNAGQALQLFDGSNQVFDAVIAQVDKKSVQVQLSAGQLEDRESPLNL
HLGQVISRGEKMEFTIQKSIELGVNVITPLFSERCGVALSGERLAKKIQQWQKIAIAACEQCGRNRIPEIREAMQLEAWC
AEQDGSLKLNLHPRASHSINTLPLPVENVRLLIGPEGGLSTGEIAMTTGHGFTDILLGPRVLRTETTALTAITALQVRFG
DLG
>Mature_243_residues
MRIPRIYHPQPLIDHAEIFLSEDAANHIGRVLRMNAGQALQLFDGSNQVFDAVIAQVDKKSVQVQLSAGQLEDRESPLNL
HLGQVISRGEKMEFTIQKSIELGVNVITPLFSERCGVALSGERLAKKIQQWQKIAIAACEQCGRNRIPEIREAMQLEAWC
AEQDGSLKLNLHPRASHSINTLPLPVENVRLLIGPEGGLSTGEIAMTTGHGFTDILLGPRVLRTETTALTAITALQVRFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=81.8930041152263, Blast_Score=410, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26682; Mature: 26682

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIYHPQPLIDHAEIFLSEDAANHIGRVLRMNAGQALQLFDGSNQVFDAVIAQVDKK
CCCCCCCCCCCCCCHHHHEECCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCC
SVQVQLSAGQLEDRESPLNLHLGQVISRGEKMEFTIQKSIELGVNVITPLFSERCGVALS
CEEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHCCEEEC
GERLAKKIQQWQKIAIAACEQCGRNRIPEIREAMQLEAWCAEQDGSLKLNLHPRASHSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
TLPLPVENVRLLIGPEGGLSTGEIAMTTGHGFTDILLGPRVLRTETTALTAITALQVRFG
CCCCCHHCEEEEECCCCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHEEECC
DLG
CCC
>Mature Secondary Structure
MRIPRIYHPQPLIDHAEIFLSEDAANHIGRVLRMNAGQALQLFDGSNQVFDAVIAQVDKK
CCCCCCCCCCCCCCHHHHEECCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHCCC
SVQVQLSAGQLEDRESPLNLHLGQVISRGEKMEFTIQKSIELGVNVITPLFSERCGVALS
CEEEEEECCCCCCCCCCCCCHHHHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHHCCEEEC
GERLAKKIQQWQKIAIAACEQCGRNRIPEIREAMQLEAWCAEQDGSLKLNLHPRASHSIN
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCC
TLPLPVENVRLLIGPEGGLSTGEIAMTTGHGFTDILLGPRVLRTETTALTAITALQVRFG
CCCCCHHCEEEEECCCCCCCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHEEECC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]