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The map label for this gene is speE [H]

Identifier: 157372235

GI number: 157372235

Start: 4435891

End: 4436754

Strand: Direct

Name: speE [H]

Synonym: Spro_4001

Alternate gene names: 157372235

Gene position: 4435891-4436754 (Clockwise)

Preceding gene: 157372234

Following gene: 157372236

Centisome position: 81.41

GC content: 54.98

Gene sequence:

>864_bases
ATGACCCAGAAAGAAATTTGGTATGAAACGCTGCATGCCAACTTCGGCCAGTACTTCTCGGTAGAGAACGTACTGTATCG
TGAGAAGACCGAGCATCAGGATTTGGTGATTTTTGAAAACCCGGTTCTGGGGCGCGTGATGGCGCTCGACGGCGTGGTGC
AAACCACCGAGCGCGATGAGTTCATCTATCACGAAATGATGACCCACGTACCGCTGCTGGCCCACGGCCAGGCGAAGAAA
GTGCTGATCATCGGCGGTGGCGACGGCGCCATGCTGCGCGAAGTCAGCCGCCATCAGGGCGTAGAGCAGATCACCATGGT
TGAGATCGACGCCGGTGTGGTCGAGTTCTGTCGCCAATATCTACCTAACCACAATGCCGGTGCTTATGACGATCCGCGCT
TCAAGCTGGTGATCGACGACGGCGTCAACTTTGTTAATCAGACCGATGAAAAATTCGATGTGATTATCTCTGACTGTACC
GATCCCATCGGCCCCGGCGAAAGTCTGTTCACCTCAGCATTCTACGAAGGCTGCGCGCGCTGCCTGAACGAAGGCGGCAT
TTTTGTTGCGCAGAACGGCGTGTGCTTCCTGCAGCAAGACGAAGCGGTCAATAGCCACACCAGGCTGAGCCAGTACTTCA
AGGACGTCAGCTTCTATCAGGCGGCGATCCCGACCTATTACGGCGGTATCATGACCTTCGCCTGGGCGAGCCAGGATCCG
GCGTTGCGCCAGCTTGATCTGACCACGCTGCAACATCGCTTTCATCAAAGCGGCTTAAACTGTCGTTATTACAACCCGGC
AATTCACGCTGGCAGCTTTGCTCTGCCACAGTATTTGCTCGACGCACTGAACGTTTCACGTTAA

Upstream 100 bases:

>100_bases
TTGCGCTACCTCTGTTTGAAATTCAGCCGTGCATCACCAGGCAGAGTTAGCTATGATGTTGCGCCAATTTTTCATGGCTG
TTATTACGGAGTCCCCGCAC

Downstream 100 bases:

>100_bases
GCGAGAAGATAAAGGAGGTGAACCAAATTGCATAAGCTAAAACTGCACGGCTTCAACAACCTGACCAAGAGCCTGAGTTT
TTGTATTTACGATATTTGTT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTQKEIWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPVLGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKK
VLIIGGGDGAMLREVSRHQGVEQITMVEIDAGVVEFCRQYLPNHNAGAYDDPRFKLVIDDGVNFVNQTDEKFDVIISDCT
DPIGPGESLFTSAFYEGCARCLNEGGIFVAQNGVCFLQQDEAVNSHTRLSQYFKDVSFYQAAIPTYYGGIMTFAWASQDP
ALRQLDLTTLQHRFHQSGLNCRYYNPAIHAGSFALPQYLLDALNVSR

Sequences:

>Translated_287_residues
MTQKEIWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPVLGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKK
VLIIGGGDGAMLREVSRHQGVEQITMVEIDAGVVEFCRQYLPNHNAGAYDDPRFKLVIDDGVNFVNQTDEKFDVIISDCT
DPIGPGESLFTSAFYEGCARCLNEGGIFVAQNGVCFLQQDEAVNSHTRLSQYFKDVSFYQAAIPTYYGGIMTFAWASQDP
ALRQLDLTTLQHRFHQSGLNCRYYNPAIHAGSFALPQYLLDALNVSR
>Mature_286_residues
TQKEIWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPVLGRVMALDGVVQTTERDEFIYHEMMTHVPLLAHGQAKKV
LIIGGGDGAMLREVSRHQGVEQITMVEIDAGVVEFCRQYLPNHNAGAYDDPRFKLVIDDGVNFVNQTDEKFDVIISDCTD
PIGPGESLFTSAFYEGCARCLNEGGIFVAQNGVCFLQQDEAVNSHTRLSQYFKDVSFYQAAIPTYYGGIMTFAWASQDPA
LRQLDLTTLQHRFHQSGLNCRYYNPAIHAGSFALPQYLLDALNVSR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=284, Percent_Identity=36.2676056338028, Blast_Score=197, Evalue=9e-51,
Organism=Escherichia coli, GI1786312, Length=283, Percent_Identity=80.9187279151943, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI25154106, Length=274, Percent_Identity=40.1459854014599, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI212645616, Length=322, Percent_Identity=34.1614906832298, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6323175, Length=292, Percent_Identity=38.013698630137, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6325326, Length=282, Percent_Identity=35.8156028368794, Blast_Score=186, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24645443, Length=281, Percent_Identity=38.4341637010676, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI21355941, Length=218, Percent_Identity=36.697247706422, Blast_Score=149, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32438; Mature: 32307

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKEIWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPVLGRVMALDGVVQTTERDE
CCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCHH
FIYHEMMTHVPLLAHGQAKKVLIIGGGDGAMLREVSRHQGVEQITMVEIDAGVVEFCRQY
HHHHHHHHHCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGAYDDPRFKLVIDDGVNFVNQTDEKFDVIISDCTDPIGPGESLFTSAFYEGCAR
CCCCCCCCCCCCCEEEEEECCCCHHCCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHH
CLNEGGIFVAQNGVCFLQQDEAVNSHTRLSQYFKDVSFYQAAIPTYYGGIMTFAWASQDP
HHCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
ALRQLDLTTLQHRFHQSGLNCRYYNPAIHAGSFALPQYLLDALNVSR
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQKEIWYETLHANFGQYFSVENVLYREKTEHQDLVIFENPVLGRVMALDGVVQTTERDE
CCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCHH
FIYHEMMTHVPLLAHGQAKKVLIIGGGDGAMLREVSRHQGVEQITMVEIDAGVVEFCRQY
HHHHHHHHHCCEEECCCCCEEEEEECCCHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHH
LPNHNAGAYDDPRFKLVIDDGVNFVNQTDEKFDVIISDCTDPIGPGESLFTSAFYEGCAR
CCCCCCCCCCCCCEEEEEECCCCHHCCCCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHH
CLNEGGIFVAQNGVCFLQQDEAVNSHTRLSQYFKDVSFYQAAIPTYYGGIMTFAWASQDP
HHCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCC
ALRQLDLTTLQHRFHQSGLNCRYYNPAIHAGSFALPQYLLDALNVSR
CHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA