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The map label for this gene is brnQ [H]

Identifier: 157372187

GI number: 157372187

Start: 4379129

End: 4380448

Strand: Direct

Name: brnQ [H]

Synonym: Spro_3953

Alternate gene names: 157372187

Gene position: 4379129-4380448 (Clockwise)

Preceding gene: 157372186

Following gene: 157372189

Centisome position: 80.37

GC content: 62.58

Gene sequence:

>1320_bases
ATGACAACACGCTTATCTCCCAAAGACATTATCGCCCTGGGCTTTATGACCTTCGCCCTGTTTGTCGGCGCCGGCAATAT
CATTTTCCCGCCAATGGTGGGCCTGCAGTCCGGTGAGCATCTGTGGCTGGCCGCCGCAGGCTTTATCGTCACCGCCGTCG
TCCTGCCGGTGATCGCGGTGATTGCGCTGGCGCGTGTCGGCGGCAGCATCAGCCTGTTGACCGCACCAATCGGCCGCAGG
GCCGGCCTGCTGCTGGCTACCCTCTGCTATCTGGCGCTCGGCCCGCTGTTCGCCACACCGCGCACCGCCAACGTTTCTTT
CGCTTTGGGCATCGCGCCTTTTACCGGCGACGGCGCCCTGCAGCAGTTTATCTACAGCCTGCTGTTCTTCACGCTGGCAA
TGATTATCTCGCTGTACCCCGGGCGGCTGCTGGATAACGTCGGCCATATTCTGGCACCGCTGAAGATCCTCGCGCTGGCC
GCACTGGGCGTCGCCGCGTTGATTTGGCCCGCCGGCGCGCCCATCCTCGCCAGCGGCAGCTATCAGGACGCGGCGTTCTC
CACCGGTTTTGTGCAGGGATATCTGACGATGGACACGCTGTCGGCGCTGATGTTCGGCTCGATTATCGTTACCGCCGCAC
GGTCGCGCGGCGTCAACGATCGGGGTTTGTTGATGCGCTACACTCTGTGGGCCAGCCTGATTGCCGGCATCGGCCTGACA
CTGGTTTATATCTGCATGTTCAAGCTGGGCGCCGGCAGCGGCGGCCTGGTTGCCGACGGGCAAGACGGCGCGGCAATCCT
GCATGCCTATGTACAGCACACCTTCGGCGATTTGGGCAGCGTCTTTATGGCCATCCTGATGTTCATCGCCTGTCTGGTGA
CTGCGGTGGGCATGACCTGCGCCTGCGCCGATTTCTTCTCCCGCTATCTGCCGCTGTCTTACCGTTCACTGGTGGTCCTC
CTCGCTGCGTTCGCCATGCTGGTGTCCAATCTCGGGCTGGCCAATCTGATCCGCGTATCGATTCCGGTGCTGACGGCCAT
CTATCCTCCGTGCATCGCCCTGGTATTGCTCAGCTTTAGCCAGAGCCGTTGGCGCAGCGCCAACCGCGTGTTCACACCGG
TGATCGCCACCAGTCTGCTGCTTGGCCTCGCCGACGGAATAAAGGCCTCCAGCTTCAGCGGCCTGCTGCCGGCCTGGTTC
GATCAACTGCCGTTGGCGGATCAGGGATTGGTTTGGTTGCAGCCGACGCTGTTAGTGCTGTTGCTGGCGGCGGTTTACGA
TCGGTTGCGCGCACCGCGCCGCGCCGGGACGATGGCCTAA

Upstream 100 bases:

>100_bases
ATGCCGAAATTTTTATGGGTGCCCGTTTGCCCATCGCGCCTGCCCTTGAGTTTACCGGCGCCAGGTTGTTTTTATCGTAC
ACAGGCAAAGCAATACCGTT

Downstream 100 bases:

>100_bases
AATAAAAAAAACCGGTCAATGACCGGTTTTTTTATCAGCTTGCGAAGGACCGCTTATTTCAGCAGTGCCTGCGCCTTGGC
CACCACGTTGTCCACGGTGA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTTRLSPKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHLWLAAAGFIVTAVVLPVIAVIALARVGGSISLLTAPIGRR
AGLLLATLCYLALGPLFATPRTANVSFALGIAPFTGDGALQQFIYSLLFFTLAMIISLYPGRLLDNVGHILAPLKILALA
ALGVAALIWPAGAPILASGSYQDAAFSTGFVQGYLTMDTLSALMFGSIIVTAARSRGVNDRGLLMRYTLWASLIAGIGLT
LVYICMFKLGAGSGGLVADGQDGAAILHAYVQHTFGDLGSVFMAILMFIACLVTAVGMTCACADFFSRYLPLSYRSLVVL
LAAFAMLVSNLGLANLIRVSIPVLTAIYPPCIALVLLSFSQSRWRSANRVFTPVIATSLLLGLADGIKASSFSGLLPAWF
DQLPLADQGLVWLQPTLLVLLLAAVYDRLRAPRRAGTMA

Sequences:

>Translated_439_residues
MTTRLSPKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHLWLAAAGFIVTAVVLPVIAVIALARVGGSISLLTAPIGRR
AGLLLATLCYLALGPLFATPRTANVSFALGIAPFTGDGALQQFIYSLLFFTLAMIISLYPGRLLDNVGHILAPLKILALA
ALGVAALIWPAGAPILASGSYQDAAFSTGFVQGYLTMDTLSALMFGSIIVTAARSRGVNDRGLLMRYTLWASLIAGIGLT
LVYICMFKLGAGSGGLVADGQDGAAILHAYVQHTFGDLGSVFMAILMFIACLVTAVGMTCACADFFSRYLPLSYRSLVVL
LAAFAMLVSNLGLANLIRVSIPVLTAIYPPCIALVLLSFSQSRWRSANRVFTPVIATSLLLGLADGIKASSFSGLLPAWF
DQLPLADQGLVWLQPTLLVLLLAAVYDRLRAPRRAGTMA
>Mature_438_residues
TTRLSPKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHLWLAAAGFIVTAVVLPVIAVIALARVGGSISLLTAPIGRRA
GLLLATLCYLALGPLFATPRTANVSFALGIAPFTGDGALQQFIYSLLFFTLAMIISLYPGRLLDNVGHILAPLKILALAA
LGVAALIWPAGAPILASGSYQDAAFSTGFVQGYLTMDTLSALMFGSIIVTAARSRGVNDRGLLMRYTLWASLIAGIGLTL
VYICMFKLGAGSGGLVADGQDGAAILHAYVQHTFGDLGSVFMAILMFIACLVTAVGMTCACADFFSRYLPLSYRSLVVLL
AAFAMLVSNLGLANLIRVSIPVLTAIYPPCIALVLLSFSQSRWRSANRVFTPVIATSLLLGLADGIKASSFSGLLPAWFD
QLPLADQGLVWLQPTLLVLLLAAVYDRLRAPRRAGTMA

Specific function: Component of the LIV-II transport system for branched- chain amino acids. This LIV-II transport system may be H(+)- coupled [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=428, Percent_Identity=64.9532710280374, Blast_Score=572, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46221; Mature: 46090

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRLSPKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHLWLAAAGFIVTAVVLPVIAV
CCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
IALARVGGSISLLTAPIGRRAGLLLATLCYLALGPLFATPRTANVSFALGIAPFTGDGAL
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHH
QQFIYSLLFFTLAMIISLYPGRLLDNVGHILAPLKILALAALGVAALIWPAGAPILASGS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
YQDAAFSTGFVQGYLTMDTLSALMFGSIIVTAARSRGVNDRGLLMRYTLWASLIAGIGLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LVYICMFKLGAGSGGLVADGQDGAAILHAYVQHTFGDLGSVFMAILMFIACLVTAVGMTC
HHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACADFFSRYLPLSYRSLVVLLAAFAMLVSNLGLANLIRVSIPVLTAIYPPCIALVLLSFS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRWRSANRVFTPVIATSLLLGLADGIKASSFSGLLPAWFDQLPLADQGLVWLQPTLLVL
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
LLAAVYDRLRAPRRAGTMA
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TTRLSPKDIIALGFMTFALFVGAGNIIFPPMVGLQSGEHLWLAAAGFIVTAVVLPVIAV
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
IALARVGGSISLLTAPIGRRAGLLLATLCYLALGPLFATPRTANVSFALGIAPFTGDGAL
HHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHH
QQFIYSLLFFTLAMIISLYPGRLLDNVGHILAPLKILALAALGVAALIWPAGAPILASGS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
YQDAAFSTGFVQGYLTMDTLSALMFGSIIVTAARSRGVNDRGLLMRYTLWASLIAGIGLT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
LVYICMFKLGAGSGGLVADGQDGAAILHAYVQHTFGDLGSVFMAILMFIACLVTAVGMTC
HHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACADFFSRYLPLSYRSLVVLLAAFAMLVSNLGLANLIRVSIPVLTAIYPPCIALVLLSFS
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSRWRSANRVFTPVIATSLLLGLADGIKASSFSGLLPAWFDQLPLADQGLVWLQPTLLVL
HHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHH
LLAAVYDRLRAPRRAGTMA
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]