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The map label for this gene is lepA [H]

Identifier: 157371906

GI number: 157371906

Start: 4063276

End: 4065072

Strand: Reverse

Name: lepA [H]

Synonym: Spro_3671

Alternate gene names: 157371906

Gene position: 4065072-4063276 (Counterclockwise)

Preceding gene: 157371908

Following gene: 157371905

Centisome position: 74.6

GC content: 55.93

Gene sequence:

>1797_bases
ATGAAGCATATACGAAATTTCTCCATTATTGCCCACATTGACCACGGTAAGTCGACGCTGTCCGACCGTATTATCCAAAT
TTGCGGTGGCTTGACCGAACGTGAAATGGCCGCGCAGGTGCTCGATTCTATGGATCTTGAGCGCGAGCGTGGCATTACCA
TCAAAGCGCAGAGCGTGACGCTGGATTATAAGGCGCAGGACGGCCAAACCTACCAGCTCAACTTTATCGACACCCCTGGA
CACGTTGACTTCTCCTACGAGGTTTCCCGTTCACTGGCCGCCTGTGAAGGTGCACTGCTGGTGGTCGATGCCGGGCAGGG
CGTAGAAGCCCAGACGCTGGCCAACTGCTACACCGCGCTGGATATGAATCTGGAAGTGGTGCCGGTACTGAACAAAATTG
ACTTGCCGGCAGCCGATCCCGATCGCGCCGCGCAGGAAATTGAAGACATCGTTGGCATCGACGCTACCGATGCGGTGCGT
TGCTCGGCCAAAACCGGCGTTGGCGTACCAGAAGTGCTGGAACGCCTGGTGCGTGATATTCCGGGGCCGGCAGGCGATCC
GGAAGCGCCACTACAGGCACTGATCATTGACTCCTGGTTCGATAACTACCTGGGCGTAGTTTCCCTGGTGCGCGTCAAGA
ACGGTACCATGCGCAAGGGCGACAAGATCAAGGTCATGAGCACCGGCCAAACCTATAATGCCGATCGTCTGGGCATCTTC
ACCCCGAAACGTGTCGATCGCGACGTGTTGAACTGTGGTGAAGTGGGCTGGTTGGTCTGTGCAATCAAAGACATCCTTGG
CGCACCGGTGGGCGATACCCTGACACTGGCTCGCCAACCGGCGGACAAAGCCTTGCCGGGCTTCAAAAAAGTGAAGCCGC
AGGTTTACGCGGGCCTGTTCCCAATCAGCTCCGACGACTATGAATCCTTCCGCGACGCGCTGGGCAAGCTGAGCCTGAAC
GACGCCTCGCTGTTCTACGAGCCAGAGAGCTCCACCGCGCTGGGCTTCGGCTTCCGCTGTGGCTTCCTCGGCCTGTTGCA
CATGGAGATCATTCAGGAACGTCTGGAGCGTGAATACGATCTGGAGCTGATCACCACCGCGCCAACGGTAGTGTATGAAG
TGGAAACCACCAGCAAAGAGACTATCTACGTTGATAGCCCATCCAAGCTGCCGCCACTCAATAACATCGAAGAGCTGCGT
GAACCGATCGCCGAATGTCACATGCTGATGCCGCAGGAATTCCTGGGTAACGTGATCACCCTGTGTATCGAGAAACGCGG
TGTGCAGACCAACATGGTTTACCACGGTAACCAGGTTGCGCTGACTTACGAAATTCCAATGGCGGAAGTGGTACTCGACT
TCTTCGACCGTCTGAAATCTACCTCGCGCGGTTATGCGTCGCTGGATTACAACTTCAAACGCTTCCAGACCTCTGACATG
GTGCGCGTCGACGTGTTGATCAACAACGAGCGCGTGGATGCGCTGGCGCTGATCACTCACCGCGACAATTCGCAGTACCG
TGGCCGTGAGCTGGTAGAGAAGATGAAAGAGCTGATCCCACGCCAGCAGTTCGATATTGCCATCCAGGCCGCTATCGGTA
CTCACATCATCGCGCGTGCGACGGTGAAACAGCTGCGTAAAAACGTTTTGGCTAAATGCTACGGCGGTGACGTCAGCCGT
AAGAAGAAGCTGCTGCAGAAGCAGAAAGACGGTAAGAAACGCATGAAGCAGGTGGGTAACGTCGAGTTGCCACAAGAAGC
GTTCCTGGCCATTCTGCACGTTGGCAAGGACAAGTAA

Upstream 100 bases:

>100_bases
TCAGGCGCAAGACGGCCGAACCCATTCACTGTCGCTTTAGGTCCATGCGGGTTTCTATAGGCGAGTAATTCAGAAATACC
AAGGCAGAAAAACTTTTATA

Downstream 100 bases:

>100_bases
GTAACAGCGATAAAGTCGCGAAACTAGGGAGTTTGCATGGCGAATATGTTTGCCCTGATCCTGGCACTGGCTACATTGGT
GACCGGGATCATCTGGGCCT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLTEREMAAQVLDSMDLERERGITIKAQSVTLDYKAQDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLEVVPVLNKIDLPAADPDRAAQEIEDIVGIDATDAVR
CSAKTGVGVPEVLERLVRDIPGPAGDPEAPLQALIIDSWFDNYLGVVSLVRVKNGTMRKGDKIKVMSTGQTYNADRLGIF
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLARQPADKALPGFKKVKPQVYAGLFPISSDDYESFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLELITTAPTVVYEVETTSKETIYVDSPSKLPPLNNIEELR
EPIAECHMLMPQEFLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
VRVDVLINNERVDALALITHRDNSQYRGRELVEKMKELIPRQQFDIAIQAAIGTHIIARATVKQLRKNVLAKCYGGDVSR
KKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDK

Sequences:

>Translated_598_residues
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLTEREMAAQVLDSMDLERERGITIKAQSVTLDYKAQDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLEVVPVLNKIDLPAADPDRAAQEIEDIVGIDATDAVR
CSAKTGVGVPEVLERLVRDIPGPAGDPEAPLQALIIDSWFDNYLGVVSLVRVKNGTMRKGDKIKVMSTGQTYNADRLGIF
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLARQPADKALPGFKKVKPQVYAGLFPISSDDYESFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLELITTAPTVVYEVETTSKETIYVDSPSKLPPLNNIEELR
EPIAECHMLMPQEFLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
VRVDVLINNERVDALALITHRDNSQYRGRELVEKMKELIPRQQFDIAIQAAIGTHIIARATVKQLRKNVLAKCYGGDVSR
KKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDK
>Mature_598_residues
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLTEREMAAQVLDSMDLERERGITIKAQSVTLDYKAQDGQTYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALDMNLEVVPVLNKIDLPAADPDRAAQEIEDIVGIDATDAVR
CSAKTGVGVPEVLERLVRDIPGPAGDPEAPLQALIIDSWFDNYLGVVSLVRVKNGTMRKGDKIKVMSTGQTYNADRLGIF
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLARQPADKALPGFKKVKPQVYAGLFPISSDDYESFRDALGKLSLN
DASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYDLELITTAPTVVYEVETTSKETIYVDSPSKLPPLNNIEELR
EPIAECHMLMPQEFLGNVITLCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
VRVDVLINNERVDALALITHRDNSQYRGRELVEKMKELIPRQQFDIAIQAAIGTHIIARATVKQLRKNVLAKCYGGDVSR
KKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=46.1285008237232, Blast_Score=588, Evalue=1e-168,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=44.7761194029851, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=44.2953020134228, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI25306287, Length=149, Percent_Identity=44.2953020134228, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=44.2953020134228, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI18390331, Length=158, Percent_Identity=37.3417721518987, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=39.5833333333333, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=41.4414414414414, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=41.4414414414414, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=41.4414414414414, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI53729339, Length=243, Percent_Identity=30.0411522633745, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729337, Length=243, Percent_Identity=30.0411522633745, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=34.5864661654135, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI217272892, Length=133, Percent_Identity=34.5864661654135, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=91.7922948073702, Blast_Score=1107, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=27.755905511811, Blast_Score=165, Evalue=7e-42,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=34.8387096774194, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=31.4102564102564, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=30.1310043668122, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1789737, Length=331, Percent_Identity=25.6797583081571, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=331, Percent_Identity=25.6797583081571, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=609, Percent_Identity=40.5582922824302, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17556745, Length=475, Percent_Identity=24.4210526315789, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=149, Percent_Identity=36.9127516778523, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=33.5294117647059, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=36.2962962962963, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=36.2962962962963, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=34.4827586206897, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=226, Percent_Identity=30.9734513274336, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141371, Length=240, Percent_Identity=27.0833333333333, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=601, Percent_Identity=45.2579034941764, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6323098, Length=158, Percent_Identity=38.6075949367089, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=42.3611111111111, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=42.3611111111111, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=37.3913043478261, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=39.3103448275862, Blast_Score=85, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=248, Percent_Identity=27.0161290322581, Blast_Score=67, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325337, Length=284, Percent_Identity=24.2957746478873, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319594, Length=284, Percent_Identity=24.2957746478873, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=601, Percent_Identity=42.7620632279534, Blast_Score=519, Evalue=1e-147,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=36.6459627329193, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.1654676258993, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=34.8993288590604, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=34.8993288590604, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=34.8993288590604, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=35.3383458646617, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=248, Percent_Identity=28.6290322580645, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66495; Mature: 66495

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLTEREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAQDGQTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
DMNLEVVPVLNKIDLPAADPDRAAQEIEDIVGIDATDAVRCSAKTGVGVPEVLERLVRDI
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PGPAGDPEAPLQALIIDSWFDNYLGVVSLVRVKNGTMRKGDKIKVMSTGQTYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLARQPADKALPGFKKVKPQVYAGLF
CCHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHCCCCHHHCCCHHHEEEE
PISSDDYESFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCC
LELITTAPTVVYEVETTSKETIYVDSPSKLPPLNNIEELREPIAECHMLMPQEFLGNVIT
EEEEEECCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINNERVDALALITHRDNSQYRGRELVEKMKELIPRQQFDIAIQAAIGTHIIARA
EEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEHHHHHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRIIQICGGLTEREMAAQVLDSMDLERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYKAQDGQTYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEECCCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
DMNLEVVPVLNKIDLPAADPDRAAQEIEDIVGIDATDAVRCSAKTGVGVPEVLERLVRDI
CCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PGPAGDPEAPLQALIIDSWFDNYLGVVSLVRVKNGTMRKGDKIKVMSTGQTYNADRLGIF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCEEECC
TPKRVDRDVLNCGEVGWLVCAIKDILGAPVGDTLTLARQPADKALPGFKKVKPQVYAGLF
CCHHHCHHHHCCCHHHHHHHHHHHHHCCCCCCCHHCCCCCCHHCCCCHHHCCCHHHEEEE
PISSDDYESFRDALGKLSLNDASLFYEPESSTALGFGFRCGFLGLLHMEIIQERLEREYD
CCCCCHHHHHHHHHCCCCCCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCC
LELITTAPTVVYEVETTSKETIYVDSPSKLPPLNNIEELREPIAECHMLMPQEFLGNVIT
EEEEEECCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHH
LCIEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQTSDM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEECCCHHHCCCCCE
VRVDVLINNERVDALALITHRDNSQYRGRELVEKMKELIPRQQFDIAIQAAIGTHIIARA
EEEEEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHCEEEEEHHHHHHHHHH
TVKQLRKNVLAKCYGGDVSRKKKLLQKQKDGKKRMKQVGNVELPQEAFLAILHVGKDK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA