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The map label for this gene is galS [H]

Identifier: 157371851

GI number: 157371851

Start: 4000940

End: 4001977

Strand: Direct

Name: galS [H]

Synonym: Spro_3616

Alternate gene names: 157371851

Gene position: 4000940-4001977 (Clockwise)

Preceding gene: 157371837

Following gene: 157371852

Centisome position: 73.43

GC content: 56.07

Gene sequence:

>1038_bases
ATGGCCACAATGAAAGATGTTGCCCGACGGGCAGGCGTGTCGACGGCCACCGTCAGCCGGGTGATCAACAGCACCGCCTA
TGTGGAGCCGGTCACCCGCGAACGGGTTGAAAAAGCCATGCGCGAGTTTAACTATCACCGCAATGCTGCCGCGCTGGCCC
TGGCGAAAAGAAGCGGCAATATGCTGGGGCTGTTGACCGGTAATCTCGACGACCCTTTTTTCTCGCGTCTGGCACGGGGA
GTCGAAGATGTCACGCGCAAAGACGGCGTAAAATTAATGGTCTGCAGCGGCGGCCATCAGGCCGAGCTGGAGAAGAACGG
TCTCGATTTTCTGATCAATCAGGGCTGTGAAGCCATCGTGGCCCACGTGACCCGGATGAGCGATGCCGACGTTTTACGCT
ATGCCGCCCACACCCCGGCGATGGTGGTGATCAATCGCTATTTGCCTGCCATCGCCAACCGCTGCATCTGGCTGGACAAC
GTCAGCGCCTCTCAGGCCGCCACCCAGATGCTGATTCGGCACGGGCACCGCAATATCGCCTGTATCACGACGGACTTACC
CATCGATGACAGAAAACAGCGTCTGGAAGGCTATCGCAGTGCCATGAATGATGCCGGGATCTCGGTGCCGGGTAGCTGGA
TCATCAGCGTTCCCTTCAATGAGCAAGGGGGCGAATTAGCGGCAGAAAGGATCCTGGCAAACGATCACCCTTTTACCGCC
GCACTGACGTTCAATGACGTGATGGCCGCCGGTATGATGCGAACCTTTCGCCAGCGGCAGATAAAACTGCCGGAGGCTAT
CTCGATTGTCGGCTTTGATGACGTGACCCTGGCAAAATATCTTCACCCGTCATTGACCACCATGCATTATCCGATTGAGA
AAATGGCGCGACGTGCGGCTAATTTGGCGCTGCAGTTGAACTCTGCTGCCACTGTGGCACCGCAGAATAATATGTTTTCC
GCTGAATTGATCCTGCGAGACTCGGTCACCCCCCTCGAGAAAAACACCTCAAATCAAGAAAACAAGCCTGAAAAATGA

Upstream 100 bases:

>100_bases
CGAATATGGATGAATAATAGCAGCGCTGTTAGCATCAGACCCAGCTCAATTTTTTCCCTTCAACAGAAAGGGGCCACCCA
AGCCTGAAGCAGGAAATGAT

Downstream 100 bases:

>100_bases
GAGGCAACGCACACAACGCCTACTCATGAAACCGATTACATGGACAGTTGCCCAGGCTCACATCTCAATCAAAACGAATT
CATTAGGCTGGCGTCACACC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MATMKDVARRAGVSTATVSRVINSTAYVEPVTRERVEKAMREFNYHRNAAALALAKRSGNMLGLLTGNLDDPFFSRLARG
VEDVTRKDGVKLMVCSGGHQAELEKNGLDFLINQGCEAIVAHVTRMSDADVLRYAAHTPAMVVINRYLPAIANRCIWLDN
VSASQAATQMLIRHGHRNIACITTDLPIDDRKQRLEGYRSAMNDAGISVPGSWIISVPFNEQGGELAAERILANDHPFTA
ALTFNDVMAAGMMRTFRQRQIKLPEAISIVGFDDVTLAKYLHPSLTTMHYPIEKMARRAANLALQLNSAATVAPQNNMFS
AELILRDSVTPLEKNTSNQENKPEK

Sequences:

>Translated_345_residues
MATMKDVARRAGVSTATVSRVINSTAYVEPVTRERVEKAMREFNYHRNAAALALAKRSGNMLGLLTGNLDDPFFSRLARG
VEDVTRKDGVKLMVCSGGHQAELEKNGLDFLINQGCEAIVAHVTRMSDADVLRYAAHTPAMVVINRYLPAIANRCIWLDN
VSASQAATQMLIRHGHRNIACITTDLPIDDRKQRLEGYRSAMNDAGISVPGSWIISVPFNEQGGELAAERILANDHPFTA
ALTFNDVMAAGMMRTFRQRQIKLPEAISIVGFDDVTLAKYLHPSLTTMHYPIEKMARRAANLALQLNSAATVAPQNNMFS
AELILRDSVTPLEKNTSNQENKPEK
>Mature_344_residues
ATMKDVARRAGVSTATVSRVINSTAYVEPVTRERVEKAMREFNYHRNAAALALAKRSGNMLGLLTGNLDDPFFSRLARGV
EDVTRKDGVKLMVCSGGHQAELEKNGLDFLINQGCEAIVAHVTRMSDADVLRYAAHTPAMVVINRYLPAIANRCIWLDNV
SASQAATQMLIRHGHRNIACITTDLPIDDRKQRLEGYRSAMNDAGISVPGSWIISVPFNEQGGELAAERILANDHPFTAA
LTFNDVMAAGMMRTFRQRQIKLPEAISIVGFDDVTLAKYLHPSLTTMHYPIEKMARRAANLALQLNSAATVAPQNNMFSA
ELILRDSVTPLEKNTSNQENKPEK

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence (corresponding to residues 15 to 20) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=345, Percent_Identity=37.6811594202899, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=40.6060606060606, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1790194, Length=342, Percent_Identity=32.7485380116959, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=31.4199395770393, Blast_Score=175, Evalue=5e-45,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=32.1321321321321, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1787948, Length=331, Percent_Identity=30.2114803625378, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=24.9249249249249, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787906, Length=291, Percent_Identity=27.1477663230241, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI48994940, Length=319, Percent_Identity=26.3322884012539, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786540, Length=346, Percent_Identity=27.4566473988439, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=26.3665594855305, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789456, Length=346, Percent_Identity=26.0115606936416, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786268, Length=288, Percent_Identity=27.0833333333333, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790689, Length=306, Percent_Identity=24.1830065359477, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38034; Mature: 37903

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARRAGVSTATVSRVINSTAYVEPVTRERVEKAMREFNYHRNAAALALAKRSGN
CCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
MLGLLTGNLDDPFFSRLARGVEDVTRKDGVKLMVCSGGHQAELEKNGLDFLINQGCEAIV
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCHHHHHHCCHHHHH
AHVTRMSDADVLRYAAHTPAMVVINRYLPAIANRCIWLDNVSASQAATQMLIRHGHRNIA
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCEE
CITTDLPIDDRKQRLEGYRSAMNDAGISVPGSWIISVPFNEQGGELAAERILANDHPFTA
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
ALTFNDVMAAGMMRTFRQRQIKLPEAISIVGFDDVTLAKYLHPSLTTMHYPIEKMARRAA
EEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCHHHEECCHHHHHHHHH
NLALQLNSAATVAPQNNMFSAELILRDSVTPLEKNTSNQENKPEK
HEEEEECCCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ATMKDVARRAGVSTATVSRVINSTAYVEPVTRERVEKAMREFNYHRNAAALALAKRSGN
CCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCC
MLGLLTGNLDDPFFSRLARGVEDVTRKDGVKLMVCSGGHQAELEKNGLDFLINQGCEAIV
EEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHCCHHHHHHCCHHHHH
AHVTRMSDADVLRYAAHTPAMVVINRYLPAIANRCIWLDNVSASQAATQMLIRHGHRNIA
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCCCCEE
CITTDLPIDDRKQRLEGYRSAMNDAGISVPGSWIISVPFNEQGGELAAERILANDHPFTA
EEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEE
ALTFNDVMAAGMMRTFRQRQIKLPEAISIVGFDDVTLAKYLHPSLTTMHYPIEKMARRAA
EEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCHHHEECCHHHHHHHHH
NLALQLNSAATVAPQNNMFSAELILRDSVTPLEKNTSNQENKPEK
HEEEEECCCEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1619663; 9278503; 8208287; 8982002 [H]