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The map label for this gene is srlB [H]

Identifier: 157371806

GI number: 157371806

Start: 3948801

End: 3949163

Strand: Direct

Name: srlB [H]

Synonym: Spro_3571

Alternate gene names: 157371806

Gene position: 3948801-3949163 (Clockwise)

Preceding gene: 157371805

Following gene: 157371807

Centisome position: 72.47

GC content: 57.3

Gene sequence:

>363_bases
ATGAACACCATTTTCCAAACCACCATCACCCAGATTGGCGACAGTGCTCGCGAGGCGCTGCTGGACGACATGCTGATCAC
CTTCCGTGAAGGCGCACCGGCAGACATCGAAGAGTTTTGCTTCATCCATCGGCACGGCAATACCCTCGGCGAACTGCAGG
CAGGCGGCTGGATGGAGTTGGCGGAGCAGCGTTATCCCATTACCGCCGTTGGCGACGTCGCCAGCCAAAACCTGCGTGAA
CTGGGCCACCTTACCGTGCGTTTCGACGGAGAAAGTCAGGCCGAATTTCCCGGTTCTATCCACGTGGCCGGTACCACGCC
GGTTGATATCCCGTTAGGCAGTACGCTGAAATTTATCGCATAA

Upstream 100 bases:

>100_bases
GTTCTGGTCGGACGTTTCCTGACCGGCGCACCTACGGTGCTGATCGCCTGGGCCGCCTCGGCCTTCATCTACCAATAACT
TTTACTGCCAGGGGCTAGCT

Downstream 100 bases:

>100_bases
GGAGTAGACCATGTCTGAAGTAGCTGTAGTGATTGGCGGAGGCCAAACATTAGGTGCATTTTTGTGCCTGGGACTGGCGC
AGGCGGGTTACCGCGTGGCG

Product: PTS system glucitol/sorbitol-specific transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Gut; EIII-Gut; PTS system glucitol/sorbitol-specific EIIA component [H]

Number of amino acids: Translated: 120; Mature: 120

Protein sequence:

>120_residues
MNTIFQTTITQIGDSAREALLDDMLITFREGAPADIEEFCFIHRHGNTLGELQAGGWMELAEQRYPITAVGDVASQNLRE
LGHLTVRFDGESQAEFPGSIHVAGTTPVDIPLGSTLKFIA

Sequences:

>Translated_120_residues
MNTIFQTTITQIGDSAREALLDDMLITFREGAPADIEEFCFIHRHGNTLGELQAGGWMELAEQRYPITAVGDVASQNLRE
LGHLTVRFDGESQAEFPGSIHVAGTTPVDIPLGSTLKFIA
>Mature_120_residues
MNTIFQTTITQIGDSAREALLDDMLITFREGAPADIEEFCFIHRHGNTLGELQAGGWMELAEQRYPITAVGDVASQNLRE
LGHLTVRFDGESQAEFPGSIHVAGTTPVDIPLGSTLKFIA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3731

COG function: function code G; Phosphotransferase system sorbitol-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-5 domain [H]

Homologues:

Organism=Escherichia coli, GI1789056, Length=118, Percent_Identity=64.4067796610169, Blast_Score=158, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004716
- InterPro:   IPR018454
- ProDom:   PD015842 [H]

Pfam domain/function: PF03829 PTSIIA_gutA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 13088; Mature: 13088

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS51097 PTS_EIIA_TYPE_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTIFQTTITQIGDSAREALLDDMLITFREGAPADIEEFCFIHRHGNTLGELQAGGWMEL
CCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCCCCCCHHHH
AEQRYPITAVGDVASQNLRELGHLTVRFDGESQAEFPGSIHVAGTTPVDIPLGSTLKFIA
HHCCCCEEEECHHHHHHHHHHCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MNTIFQTTITQIGDSAREALLDDMLITFREGAPADIEEFCFIHRHGNTLGELQAGGWMEL
CCCHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCCCCCCHHHH
AEQRYPITAVGDVASQNLRELGHLTVRFDGESQAEFPGSIHVAGTTPVDIPLGSTLKFIA
HHCCCCEEEECHHHHHHHHHHCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferases; Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3553176; 1630305; 9205837; 9278503 [H]