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The map label for this gene is ydfI [H]

Identifier: 157371801

GI number: 157371801

Start: 3943417

End: 3944889

Strand: Direct

Name: ydfI [H]

Synonym: Spro_3566

Alternate gene names: 157371801

Gene position: 3943417-3944889 (Clockwise)

Preceding gene: 157371800

Following gene: 157371802

Centisome position: 72.37

GC content: 59.4

Gene sequence:

>1473_bases
ATGACACTTTCCAGCAACAGCCTGCTGCCGACGGCAGTTGCGCAACCGGGTTACTCACGCGAAAGGATTAAACCGCGCAT
CGTGCATCTGGGTTTTGGCGCGTTTTTCCGCGCACATCAGGCGGTATATACCGAACAGTTGGCTAACGAGCACGCCAGCG
ACTGGGGTTACTGCGTAATCAGCATGCACAACGGCCGGCAAAAAATCGCCGACCTGCAGCAACAGAATTTGTTGTATACG
GTCGCCGAAATGGACAATGACGGCTGGCACGGCCAGGTGATCGGCGTTGTCAGGCAGGCGTTACACCTGCAGGTTGACGG
ACTGGAAGCCGTGCTCGAGGCCATGGCACAGCCGGAGGTTGCCATCGTTTCACTGACCATCAGTGAGAAAGGCTACTGTT
ACCAGCCTGCCAGCGGCCAGTTACTGGCGGAACATCCTGATATCCAGCATGACATCGCCCACCCGCTGCACCCGCAATCT
GCTCCCGGCCTGATCCTGGCCGCACTGCGGCGACGGCGGGAACGAGGGCTGCCGGCGTTTGCCGTGATGTCCTGCGACAA
CATGCCGGCCAACGGTCAGGTGACCCGCAACGTGCTCAGCCAGTTGGCTCAGCGGCAGGATGCGGAACTGGCCACCTGGG
TCGAACAACAGGTCGCGTTTCCATCAACCATGGTTGACCGCATTGTCCCGGCTGTCACGGCGGAAACACGGCAACACATC
GAGGCTTTACTCGGTGGCATCGCCGATCCGGTTGCCGTCGCCTGTGAGCCCTTCTGCCAATGGGTGATCGAAGATATCTT
TCCGCAGGGACGTCCGGCATGGGAAAAGACCGGTGCCGAACTGGTCAGCGACGTGCTGCCCTACGAAGAAATGAAGCTGC
GGATGCTCAACGGCAGCCATTCGTTTCTCGCTTATCTGGGCTATTTGGCCGGTTATGCGCATATCAGCGACTGCATGCAG
GACGAAGGTTTCGTTAATGCCGCACGGCACCTGATGCTGGCAGAGCAGGCACCGACGCTGCAGGTCCACGACCTGGATTT
GACGCAATACGCAGACTCACTCCTGGCCCGTTACCGCAATGCCGCGCTAAAGCACCGAACCTGGCAGATCGCCATGGACG
GCACGCAGAAACTGCCGCAGCGCATGCTGGACACCATACGCTGGCATCTGGCGCACGGCAGCGATTTCAGCTGTCTGGCT
TTAGGCGTCGCCGGTTGGATGCGTTACGTTGGTGGCAAGGATGAACAGGGCCGGACGATTGAGATCAGCGATCCCTTAAG
TGAACGCCTCGCCGATTTGGTCGGCAACAGCCGTGAAGGCCCGCAGCGGGTGCTGGCGCTGCTGCAGCTTGATACGGTGT
TTGGCCACGATCTTCCTGATAACCCACGATTCGTCAGTGCCGTAACTGATGCCTATCTGGCGCTGCTGGCGCAGGGTGCC
AAAGAGAGTGTGGCCAGATTACTGCCAGTTTGA

Upstream 100 bases:

>100_bases
GCGCCACGCAATAGCGTGCAACCGCAAATCTCAAAACCCTAACGCATCCGGGGTGGCAAATGTCGCCCCGTTTTCTGCCC
CCACTTTCAGGAACTCCAGC

Downstream 100 bases:

>100_bases
TCTGTATAAAACGCTGGGTGAATCCACTCAGCGTTAGGTTGCTGCATTAGGAATTATCCGCCGCGGCAGCAAGATAATCT
GCTAGTATGGTGGCATCCAA

Product: mannitol dehydrogenase domain-containing protein

Products: D-fructuronate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MTLSSNSLLPTAVAQPGYSRERIKPRIVHLGFGAFFRAHQAVYTEQLANEHASDWGYCVISMHNGRQKIADLQQQNLLYT
VAEMDNDGWHGQVIGVVRQALHLQVDGLEAVLEAMAQPEVAIVSLTISEKGYCYQPASGQLLAEHPDIQHDIAHPLHPQS
APGLILAALRRRRERGLPAFAVMSCDNMPANGQVTRNVLSQLAQRQDAELATWVEQQVAFPSTMVDRIVPAVTAETRQHI
EALLGGIADPVAVACEPFCQWVIEDIFPQGRPAWEKTGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYAHISDCMQ
DEGFVNAARHLMLAEQAPTLQVHDLDLTQYADSLLARYRNAALKHRTWQIAMDGTQKLPQRMLDTIRWHLAHGSDFSCLA
LGVAGWMRYVGGKDEQGRTIEISDPLSERLADLVGNSREGPQRVLALLQLDTVFGHDLPDNPRFVSAVTDAYLALLAQGA
KESVARLLPV

Sequences:

>Translated_490_residues
MTLSSNSLLPTAVAQPGYSRERIKPRIVHLGFGAFFRAHQAVYTEQLANEHASDWGYCVISMHNGRQKIADLQQQNLLYT
VAEMDNDGWHGQVIGVVRQALHLQVDGLEAVLEAMAQPEVAIVSLTISEKGYCYQPASGQLLAEHPDIQHDIAHPLHPQS
APGLILAALRRRRERGLPAFAVMSCDNMPANGQVTRNVLSQLAQRQDAELATWVEQQVAFPSTMVDRIVPAVTAETRQHI
EALLGGIADPVAVACEPFCQWVIEDIFPQGRPAWEKTGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYAHISDCMQ
DEGFVNAARHLMLAEQAPTLQVHDLDLTQYADSLLARYRNAALKHRTWQIAMDGTQKLPQRMLDTIRWHLAHGSDFSCLA
LGVAGWMRYVGGKDEQGRTIEISDPLSERLADLVGNSREGPQRVLALLQLDTVFGHDLPDNPRFVSAVTDAYLALLAQGA
KESVARLLPV
>Mature_489_residues
TLSSNSLLPTAVAQPGYSRERIKPRIVHLGFGAFFRAHQAVYTEQLANEHASDWGYCVISMHNGRQKIADLQQQNLLYTV
AEMDNDGWHGQVIGVVRQALHLQVDGLEAVLEAMAQPEVAIVSLTISEKGYCYQPASGQLLAEHPDIQHDIAHPLHPQSA
PGLILAALRRRRERGLPAFAVMSCDNMPANGQVTRNVLSQLAQRQDAELATWVEQQVAFPSTMVDRIVPAVTAETRQHIE
ALLGGIADPVAVACEPFCQWVIEDIFPQGRPAWEKTGAELVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYAHISDCMQD
EGFVNAARHLMLAEQAPTLQVHDLDLTQYADSLLARYRNAALKHRTWQIAMDGTQKLPQRMLDTIRWHLAHGSDFSCLAL
GVAGWMRYVGGKDEQGRTIEISDPLSERLADLVGNSREGPQRVLALLQLDTVFGHDLPDNPRFVSAVTDAYLALLAQGAK
ESVARLLPV

Specific function: Unknown

COG id: COG0246

COG function: function code G; Mannitol-1-phosphate/altronate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannitol dehydrogenase family. UxuB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787823, Length=481, Percent_Identity=63.4095634095634, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1788497, Length=490, Percent_Identity=55.3061224489796, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1790779, Length=492, Percent_Identity=53.8617886178862, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1790028, Length=322, Percent_Identity=26.7080745341615, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994885, Length=476, Percent_Identity=23.5294117647059, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6324401, Length=490, Percent_Identity=36.9387755102041, Blast_Score=326, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6320765, Length=490, Percent_Identity=36.9387755102041, Blast_Score=326, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR000669
- InterPro:   IPR013118
- InterPro:   IPR023027
- InterPro:   IPR013131
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01232 Mannitol_dh; PF08125 Mannitol_dh_C [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54165; Mature: 54034

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS00974 MANNITOL_DHGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSSNSLLPTAVAQPGYSRERIKPRIVHLGFGAFFRAHQAVYTEQLANEHASDWGYCVI
CCCCCCCCCCHHHCCCCCCHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
SMHNGRQKIADLQQQNLLYTVAEMDNDGWHGQVIGVVRQALHLQVDGLEAVLEAMAQPEV
EECCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCE
AIVSLTISEKGYCYQPASGQLLAEHPDIQHDIAHPLHPQSAPGLILAALRRRRERGLPAF
EEEEEEECCCCCEECCCCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEE
AVMSCDNMPANGQVTRNVLSQLAQRQDAELATWVEQQVAFPSTMVDRIVPAVTAETRQHI
EEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EALLGGIADPVAVACEPFCQWVIEDIFPQGRPAWEKTGAELVSDVLPYEEMKLRMLNGSH
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCH
SFLAYLGYLAGYAHISDCMQDEGFVNAARHLMLAEQAPTLQVHDLDLTQYADSLLARYRN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
AALKHRTWQIAMDGTQKLPQRMLDTIRWHLAHGSDFSCLALGVAGWMRYVGGKDEQGRTI
HHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
EISDPLSERLADLVGNSREGPQRVLALLQLDTVFGHDLPDNPRFVSAVTDAYLALLAQGA
EECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCH
KESVARLLPV
HHHHHHHCCC
>Mature Secondary Structure 
TLSSNSLLPTAVAQPGYSRERIKPRIVHLGFGAFFRAHQAVYTEQLANEHASDWGYCVI
CCCCCCCCCHHHCCCCCCHHCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
SMHNGRQKIADLQQQNLLYTVAEMDNDGWHGQVIGVVRQALHLQVDGLEAVLEAMAQPEV
EECCCHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCCE
AIVSLTISEKGYCYQPASGQLLAEHPDIQHDIAHPLHPQSAPGLILAALRRRRERGLPAF
EEEEEEECCCCCEECCCCCCEEECCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEE
AVMSCDNMPANGQVTRNVLSQLAQRQDAELATWVEQQVAFPSTMVDRIVPAVTAETRQHI
EEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
EALLGGIADPVAVACEPFCQWVIEDIFPQGRPAWEKTGAELVSDVLPYEEMKLRMLNGSH
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCH
SFLAYLGYLAGYAHISDCMQDEGFVNAARHLMLAEQAPTLQVHDLDLTQYADSLLARYRN
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHH
AALKHRTWQIAMDGTQKLPQRMLDTIRWHLAHGSDFSCLALGVAGWMRYVGGKDEQGRTI
HHHHCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
EISDPLSERLADLVGNSREGPQRVLALLQLDTVFGHDLPDNPRFVSAVTDAYLALLAQGA
EECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCH
KESVARLLPV
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-mannonate; NAD(+) [C]

Specific reaction: D-mannonate + NAD(+) = D-fructuronate + NADH [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]