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The map label for this gene is gcvR [H]

Identifier: 157371747

GI number: 157371747

Start: 3885972

End: 3886658

Strand: Direct

Name: gcvR [H]

Synonym: Spro_3510

Alternate gene names: 157371747

Gene position: 3885972-3886658 (Clockwise)

Preceding gene: 157371740

Following gene: 157371748

Centisome position: 71.32

GC content: 53.71

Gene sequence:

>687_bases
ATGGTACTTTTGCTGCCTATCCAAAAGCAAGCAAACAACGGTGTTGTAAGCGCTTGGCAGGCGGTTTTTTTTATGTTTAC
CATGGTAACCCCATTGTGCACGACAGGAAGCCCCATTTTGCCTAAGCCAGATGAACACTATCTCGTGATTACCGCGCTCG
GTACCGACCGCCCCGGGATCGTCAATGCCATCACCCGCCACGTCAGCAGTTGCGGATGCAATATCGAAGATAGCCGTCTG
GCCATGCTGGGTGAGGAGTTCACGTTCATCATGCTGCTTTCCGGTAGCTGGAACGCCATAACCCTGATTGAATCTACCCT
GCCGCAAAAAGGGGCGGAGCTGGAACTGCTGATTGTGATGAAGCGCACCAACTCGCACGAGAGGCCGCCGATGCCGGCTA
CGGTGTGGGTGCAGGTTGAGGTCAAGGACTCCCCGCACATCATTGAGCGTTTTACCGATCTGTTCCACTCCAGCCAGATG
AACATTGCCGAGCTGGTGTCGCGCACCCAACCGGCAGAAAGCGACCTGCCGCCCCAGCTTCATATCCAGATCACCGCACA
CAGCCCCGGCAACGCCGATGCATCAAATATTGAGCAAGCCTTTCATCGCCTATGTACAGAATTGAAAGCACAAGGCAGTA
TTAGCGTTGTGAACTATCCACAGCATGATGAGAAAGATGGAGAGTAG

Upstream 100 bases:

>100_bases
AGGCTCGCGCGATCGACAGCACCTGTGTCGTCCATCGGCGTAACCAGTGCAACTATACTTCCCGTAAACATTGGCCGTCC
CCTCCACAAACAAGTCACTC

Downstream 100 bases:

>100_bases
TGATGACCCCATTGAAAGCCGGTGACACAGCGCCAAACTTTAGTTTGCCTGACCAGGACGGTGAGGAAATTAATCTGGCC
GACTTCCAGGGACAACGAGT

Product: glycine cleavage system transcriptional repressor

Products: NA

Alternate protein names: Gcv operon repressor [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MVLLLPIQKQANNGVVSAWQAVFFMFTMVTPLCTTGSPILPKPDEHYLVITALGTDRPGIVNAITRHVSSCGCNIEDSRL
AMLGEEFTFIMLLSGSWNAITLIESTLPQKGAELELLIVMKRTNSHERPPMPATVWVQVEVKDSPHIIERFTDLFHSSQM
NIAELVSRTQPAESDLPPQLHIQITAHSPGNADASNIEQAFHRLCTELKAQGSISVVNYPQHDEKDGE

Sequences:

>Translated_228_residues
MVLLLPIQKQANNGVVSAWQAVFFMFTMVTPLCTTGSPILPKPDEHYLVITALGTDRPGIVNAITRHVSSCGCNIEDSRL
AMLGEEFTFIMLLSGSWNAITLIESTLPQKGAELELLIVMKRTNSHERPPMPATVWVQVEVKDSPHIIERFTDLFHSSQM
NIAELVSRTQPAESDLPPQLHIQITAHSPGNADASNIEQAFHRLCTELKAQGSISVVNYPQHDEKDGE
>Mature_228_residues
MVLLLPIQKQANNGVVSAWQAVFFMFTMVTPLCTTGSPILPKPDEHYLVITALGTDRPGIVNAITRHVSSCGCNIEDSRL
AMLGEEFTFIMLLSGSWNAITLIESTLPQKGAELELLIVMKRTNSHERPPMPATVWVQVEVKDSPHIIERFTDLFHSSQM
NIAELVSRTQPAESDLPPQLHIQITAHSPGNADASNIEQAFHRLCTELKAQGSISVVNYPQHDEKDGE

Specific function: Negative transcriptional regulator of the glycine cleavage system operon (GCV). Does not autoregulate its own expression. It is not yet known how gcvR acts as a repressor. It does not seem to bind DNA. It could interact with gcvA and suppress its activato

COG id: COG2716

COG function: function code E; Glycine cleavage system regulatory protein

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082113, Length=183, Percent_Identity=82.5136612021858, Blast_Score=318, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR016867 [H]

Pfam domain/function: PF01842 ACT [H]

EC number: NA

Molecular weight: Translated: 25207; Mature: 25207

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLLLPIQKQANNGVVSAWQAVFFMFTMVTPLCTTGSPILPKPDEHYLVITALGTDRPGI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHH
VNAITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSWNAITLIESTLPQKGAELELLIVM
HHHHHHHHHHCCCCCCCCHHEECCCCEEEEEEEECCCCEEEEEEHHCCCCCCCEEEEEEE
KRTNSHERPPMPATVWVQVEVKDSPHIIERFTDLFHSSQMNIAELVSRTQPAESDLPPQL
ECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEE
HIQITAHSPGNADASNIEQAFHRLCTELKAQGSISVVNYPQHDEKDGE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MVLLLPIQKQANNGVVSAWQAVFFMFTMVTPLCTTGSPILPKPDEHYLVITALGTDRPGI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCHH
VNAITRHVSSCGCNIEDSRLAMLGEEFTFIMLLSGSWNAITLIESTLPQKGAELELLIVM
HHHHHHHHHHCCCCCCCCHHEECCCCEEEEEEEECCCCEEEEEEHHCCCCCCCEEEEEEE
KRTNSHERPPMPATVWVQVEVKDSPHIIERFTDLFHSSQMNIAELVSRTQPAESDLPPQL
ECCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEE
HIQITAHSPGNADASNIEQAFHRLCTELKAQGSISVVNYPQHDEKDGE
EEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537378; 9205837; 9278503; 2016588 [H]