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The map label for this gene is ptsI [H]

Identifier: 157371686

GI number: 157371686

Start: 3819461

End: 3821188

Strand: Direct

Name: ptsI [H]

Synonym: Spro_3449

Alternate gene names: 157371686

Gene position: 3819461-3821188 (Clockwise)

Preceding gene: 157371685

Following gene: 157371687

Centisome position: 70.1

GC content: 53.12

Gene sequence:

>1728_bases
ATGATTTCAGGCATTTTAGTATCACCGGGTATCGCTTTTGGTAAGGCTCTCCTACTGAAAGAAGATGAAATTGTCATCAA
CCGGAAGAAAATCTCTGCAGACGCTGTAGAGCAGGAAGTCCAACGTTTTCTTGATGGCCGCGCCAAGGCATCCGTGCAGT
TGGAAGCGATCAAGACCAAAGCTGGCGAAACCTTCGGCGAAGAGAAAGAAGCTATCTTCGAAGGCCATATCATGCTGTTG
GAAGACGAAGAGCTTGAGCAGGAAATCATAGCCCTAATCAAAGACGATCTGGCTTCTGCGGACGCGGCTGCCTACACCAT
CATCGAAGGCCAGGCGAAAGCGCTGGAAGAACTCGACGATGAGTACCTGAAAGAGCGTGCGGCCGACGTGCGTGACATCG
GTAAACGCCTGCTGCAGAACATTCTGGGCCTGGCAATCGTCGATCTGAGCTCAATTCAGGACGAAGTGATCCTGGTTGCT
ACCGATTTGACCCCGTCTGAGACCGCGCAGTTGAACCTGGACAAGGTTCTGGGCTTCATCACCGATCTCGGCGGCCGTAC
TTCACACACCTCCATCATGGCGCGCTCCCTGGAATTGCCGGCAATCGTGGGCACCAGCGACGTGACCAAGCAGGTGAAGA
ACGACGACTACCTGATTCTGGACGCGGTTAACAATAAAATTTACGTTAACCCAACCGCTGACGTTATCGATCAGTTGAAA
GCCGAACAGAACCAGTACGTGACCGAGAAAAACGATCTGGCCAAGCTGAAAGACCTGCCGGCGATTACGCTGGACGGTCA
TCAGGTTGAAGTCTGTGCCAACATCGGCACCGTGCGCGACGTCGCAGGTGCAGAGCGCAACGGCGCTGAAGGTGTCGGCC
TGTATCGTACCGAATTCCTGTTCATGGACCGCGACTCACTGCCGACCGAAGACGAGCAGTTCCAGGCCTATAAAGCCGTT
GCGGAAGCCGTGGGCTCACAGGCAGTGATCGTACGCACCATGGACATCGGCGGCGACAAAGACCTGCCGTACATGAACCT
GCCGAAAGAAGAGAACCCATTCCTCGGCTGGCGCGCCATCCGTATCGCCATGGATCGCAGAGAAATCCTGCACGCCCAGC
TGCGTGCCATCCTGCGCGCCTCCGCGTTCGGTAAATTGCGCATCATGTTCCCGATGATCATTTCAGTAGAAGAAGTTCGC
GATCTGAAAGGCGAAATCGAGACGCTGAAAGCGCAATTGCGTGAAGAAGCCAAGGCGTTTGACGAAACAATTGAAGTGGG
CGTGATGGTGGAAACACCGGCCGCGGCGGTGATTGCTCATCACCTGGCGAAAGAAGTCGACTTCTTTAGTATTGGGACAA
ACGATCTAACCCAGTATACTCTGGCGGTAGATCGCGGCAACGAGCTGATTTCTCATCTCTATAACCCGATGTCCCCATCA
GTGCTTGGCCTGATCAAACAGGTTATTGATGCATCTCATGCAGAAGGCAAGTGGACCGGAATGTGCGGTGAACTGGCTGG
CGATGAGCGTGCTACACTGTTGTTATTGGGCATGGGGCTGGATGAGTTCAGCATGAGTGCGATTTCAATCCCGCGCATCA
AGAAAATTATACGTAATACTAATTTCGAAGATGTGAAGGCGTTGGCAGCGCAAGCCTTGGCACAGCCAACGGCGCAAGAT
CTGATGAATTGCGTGAATAAATTCATCGAAGAAAAGACGCTCTGCTAA

Upstream 100 bases:

>100_bases
GACGAGCAGAAAGCCGTTGAACACTTGGTAAAACTGATGGCAGAGCTTGAGTAATCGGCCCGTCTTCTTAGTTAACACCA
GTCAAGAGTAAGGTAGGGTT

Downstream 100 bases:

>100_bases
ACTTCCACGACACTGGAACGCCGCCCAATTTAATGCTTAGGAGAAGATCATGGGTTTGTTCGATAAATTGAAATCTCTGG
TTTCTGATGACAAGAAAGAC

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MISGILVSPGIAFGKALLLKEDEIVINRKKISADAVEQEVQRFLDGRAKASVQLEAIKTKAGETFGEEKEAIFEGHIMLL
EDEELEQEIIALIKDDLASADAAAYTIIEGQAKALEELDDEYLKERAADVRDIGKRLLQNILGLAIVDLSSIQDEVILVA
TDLTPSETAQLNLDKVLGFITDLGGRTSHTSIMARSLELPAIVGTSDVTKQVKNDDYLILDAVNNKIYVNPTADVIDQLK
AEQNQYVTEKNDLAKLKDLPAITLDGHQVEVCANIGTVRDVAGAERNGAEGVGLYRTEFLFMDRDSLPTEDEQFQAYKAV
AEAVGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAIRIAMDRREILHAQLRAILRASAFGKLRIMFPMIISVEEVR
DLKGEIETLKAQLREEAKAFDETIEVGVMVETPAAAVIAHHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDVKALAAQALAQPTAQD
LMNCVNKFIEEKTLC

Sequences:

>Translated_575_residues
MISGILVSPGIAFGKALLLKEDEIVINRKKISADAVEQEVQRFLDGRAKASVQLEAIKTKAGETFGEEKEAIFEGHIMLL
EDEELEQEIIALIKDDLASADAAAYTIIEGQAKALEELDDEYLKERAADVRDIGKRLLQNILGLAIVDLSSIQDEVILVA
TDLTPSETAQLNLDKVLGFITDLGGRTSHTSIMARSLELPAIVGTSDVTKQVKNDDYLILDAVNNKIYVNPTADVIDQLK
AEQNQYVTEKNDLAKLKDLPAITLDGHQVEVCANIGTVRDVAGAERNGAEGVGLYRTEFLFMDRDSLPTEDEQFQAYKAV
AEAVGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAIRIAMDRREILHAQLRAILRASAFGKLRIMFPMIISVEEVR
DLKGEIETLKAQLREEAKAFDETIEVGVMVETPAAAVIAHHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDVKALAAQALAQPTAQD
LMNCVNKFIEEKTLC
>Mature_575_residues
MISGILVSPGIAFGKALLLKEDEIVINRKKISADAVEQEVQRFLDGRAKASVQLEAIKTKAGETFGEEKEAIFEGHIMLL
EDEELEQEIIALIKDDLASADAAAYTIIEGQAKALEELDDEYLKERAADVRDIGKRLLQNILGLAIVDLSSIQDEVILVA
TDLTPSETAQLNLDKVLGFITDLGGRTSHTSIMARSLELPAIVGTSDVTKQVKNDDYLILDAVNNKIYVNPTADVIDQLK
AEQNQYVTEKNDLAKLKDLPAITLDGHQVEVCANIGTVRDVAGAERNGAEGVGLYRTEFLFMDRDSLPTEDEQFQAYKAV
AEAVGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAIRIAMDRREILHAQLRAILRASAFGKLRIMFPMIISVEEVR
DLKGEIETLKAQLREEAKAFDETIEVGVMVETPAAAVIAHHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDVKALAAQALAQPTAQD
LMNCVNKFIEEKTLC

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=86.9565217391304, Blast_Score=997, Evalue=0.0,
Organism=Escherichia coli, GI1788726, Length=574, Percent_Identity=38.6759581881533, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI48994992, Length=512, Percent_Identity=39.0625, Blast_Score=335, Evalue=7e-93,
Organism=Escherichia coli, GI1789193, Length=516, Percent_Identity=34.8837209302326, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI1787994, Length=450, Percent_Identity=26.8888888888889, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI226510935, Length=214, Percent_Identity=27.1028037383178, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63206; Mature: 63206

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGILVSPGIAFGKALLLKEDEIVINRKKISADAVEQEVQRFLDGRAKASVQLEAIKTK
CCCCEEECCCHHHHHHHEEECCCEEEEHHHCCHHHHHHHHHHHHCCCCCCEEEEEEHHHH
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDDLASADAAAYTIIEGQAKALEELDD
CCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCHHEEEEECCHHHHHHHHHH
EYLKERAADVRDIGKRLLQNILGLAIVDLSSIQDEVILVATDLTPSETAQLNLDKVLGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCHHHHHHHH
TDLGGRTSHTSIMARSLELPAIVGTSDVTKQVKNDDYLILDAVNNKIYVNPTADVIDQLK
HHCCCCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHH
AEQNQYVTEKNDLAKLKDLPAITLDGHQVEVCANIGTVRDVAGAERNGAEGVGLYRTEFL
HHHHHCCCCHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCCEEEEEEE
FMDRDSLPTEDEQFQAYKAVAEAVGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAI
EEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
RIAMDRREILHAQLRAILRASAFGKLRIMFPMIISVEEVRDLKGEIETLKAQLREEAKAF
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DETIEVGVMVETPAAAVIAHHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
HHHHEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCCHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHCC
NFEDVKALAAQALAQPTAQDLMNCVNKFIEEKTLC
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISGILVSPGIAFGKALLLKEDEIVINRKKISADAVEQEVQRFLDGRAKASVQLEAIKTK
CCCCEEECCCHHHHHHHEEECCCEEEEHHHCCHHHHHHHHHHHHCCCCCCEEEEEEHHHH
AGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDDLASADAAAYTIIEGQAKALEELDD
CCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHHHHHCCCCHHEEEEECCHHHHHHHHHH
EYLKERAADVRDIGKRLLQNILGLAIVDLSSIQDEVILVATDLTPSETAQLNLDKVLGFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCHHHHHHHH
TDLGGRTSHTSIMARSLELPAIVGTSDVTKQVKNDDYLILDAVNNKIYVNPTADVIDQLK
HHCCCCCCHHHHHHHHCCCCEEECCHHHHHHHCCCCEEEEEECCCEEEECCHHHHHHHHH
AEQNQYVTEKNDLAKLKDLPAITLDGHQVEVCANIGTVRDVAGAERNGAEGVGLYRTEFL
HHHHHCCCCHHHHHHHHCCCEEEECCCEEEEEECCCCHHHHCCCCCCCCCCCCEEEEEEE
FMDRDSLPTEDEQFQAYKAVAEAVGSQAVIVRTMDIGGDKDLPYMNLPKEENPFLGWRAI
EEECCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
RIAMDRREILHAQLRAILRASAFGKLRIMFPMIISVEEVRDLKGEIETLKAQLREEAKAF
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DETIEVGVMVETPAAAVIAHHLAKEVDFFSIGTNDLTQYTLAVDRGNELISHLYNPMSPS
HHHHEEEEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEECCCHHHHHHHCCCCCHH
VLGLIKQVIDASHAEGKWTGMCGELAGDERATLLLLGMGLDEFSMSAISIPRIKKIIRNT
HHHHHHHHHCCCCCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHCC
NFEDVKALAAQALAQPTAQDLMNCVNKFIEEKTLC
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2457575; 2960675; 9205837; 9278503; 2411636; 3290198; 9298646; 7876255; 8805571; 9054557; 9541412; 10048929 [H]