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The map label for this gene is yfbQ [H]

Identifier: 157371547

GI number: 157371547

Start: 3666016

End: 3667230

Strand: Direct

Name: yfbQ [H]

Synonym: Spro_3310

Alternate gene names: 157371547

Gene position: 3666016-3667230 (Clockwise)

Preceding gene: 157371532

Following gene: 157371548

Centisome position: 67.28

GC content: 55.8

Gene sequence:

>1215_bases
ATGTCCCCCATTGAGAAATCCAGCAAACTGGACAACGTCTGCTATGACATTCGCGGCCCGGTGCTCAAAGAAGCTAAACG
TCTTGAAGAAGAAGGCCATAAAGTCCTCAAACTGAACATTGGTAACCCTGCCCCGTTTGGTTTTGACGCCCCTGACGAAA
TCCTGGTCGACGTGATCCGCAATCTGCCCACGGCGCAAGGCTATTCCGACTCCAAAGGGCTCTTCTCGGCACGTAAAGCG
ATCATGCAGCACTACCAGGCGCGCGACATACGTGACGTGACCGTTGAGGATATCTACATCGGCAATGGGGTTTCCGAGCT
TATCGTGCAATCCATGCAGGCGCTGCTCAACAGCGGCGACGAAATGCTGGTGCCGGCACCGGATTATCCGCTGTGGACCG
CGGCGGTTTCGCTGTCCGGCGGCAAAGCCGTGCACTACCTGTGTGATGAAGAAGCCGGCTGGTTCCCGGATCTGGATGAT
ATCATCAGCAAGATCACCCCACGCACCCGTGGCATTGTGATCATCAACCCGAATAACCCGACCGGCGCGGTCTATAGCAA
AGCACTGCTGGAGCAGATTGTTGAGATCGCCCGTCAGCACAACCTGATCATCTTCTCCGATGAGATCTACGACAAAATTC
TGTATGACGAAGCCGAGCACCATTCGATCGCTGCGCTGGCGCCGGATCTGCTGACCGTCACCTTCAACGGGCTGTCCAAA
ACTTACCGCGTGGCCGGTTTCCGCCAGGGGTGGATGGTTCTGAACGGGCCAAAGAAACATGCCAAGGGCTATATCGAAGG
GCTGGAAATGCTGGCGTCGATGCGTCTGTGCGCCAACGTGCCGATGCAGCACGCGATCCAAACGGCGTTGGGCGGTTACC
AGAGTATCAATGAATTTATTCAACCCGGCGGCCGTCTGTATGAACAGCGCAACCGCACCTGGGAGCTGTTGAACGAAATC
CCAGGGGTTTCCTGCGTGAAACCTCAGGGCGCGTTGTATATGTTCCCGCGCATCGATGCCAAACGCTTTAATATTCATGA
TGACCAGAAATTGGTGCTGGATCTGCTGCTGCAGGAAAAAGTGCTGCTGGTGCAGGGCAGCGCGTTCAACTGGCCGTACC
CGGACCACGTGCGCATAGTAACGCTGCCACGTGTGGATGAACTGGAAATGGCGGTGGGCAAACTGGGTCGTTTCCTGGAA
GGTTATCGCCAGTAG

Upstream 100 bases:

>100_bases
TGCACAACCCTTCAAAAGCGTCAGCGCCAGGAGTACATTGGGCAGGTTGCGGTGTTCCACGGTAGGAACGCCCCCATCCC
AGCAAAAGGCTCAACTTTCT

Downstream 100 bases:

>100_bases
TCTCTGTGGGCGCAGCATGCTGCGCCCCTTCCCCTCCGCCGAATCCTAACCGCACGGCCCGAGTTGCATAACCCGCCATC
ACTGCTCACAATGGAGTTCT

Product: aminotransferase AlaT

Products: oxaloacetate; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSPIEKSSKLDNVCYDIRGPVLKEAKRLEEEGHKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYSDSKGLFSARKA
IMQHYQARDIRDVTVEDIYIGNGVSELIVQSMQALLNSGDEMLVPAPDYPLWTAAVSLSGGKAVHYLCDEEAGWFPDLDD
IISKITPRTRGIVIINPNNPTGAVYSKALLEQIVEIARQHNLIIFSDEIYDKILYDEAEHHSIAALAPDLLTVTFNGLSK
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPMQHAIQTALGGYQSINEFIQPGGRLYEQRNRTWELLNEI
PGVSCVKPQGALYMFPRIDAKRFNIHDDQKLVLDLLLQEKVLLVQGSAFNWPYPDHVRIVTLPRVDELEMAVGKLGRFLE
GYRQ

Sequences:

>Translated_404_residues
MSPIEKSSKLDNVCYDIRGPVLKEAKRLEEEGHKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYSDSKGLFSARKA
IMQHYQARDIRDVTVEDIYIGNGVSELIVQSMQALLNSGDEMLVPAPDYPLWTAAVSLSGGKAVHYLCDEEAGWFPDLDD
IISKITPRTRGIVIINPNNPTGAVYSKALLEQIVEIARQHNLIIFSDEIYDKILYDEAEHHSIAALAPDLLTVTFNGLSK
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPMQHAIQTALGGYQSINEFIQPGGRLYEQRNRTWELLNEI
PGVSCVKPQGALYMFPRIDAKRFNIHDDQKLVLDLLLQEKVLLVQGSAFNWPYPDHVRIVTLPRVDELEMAVGKLGRFLE
GYRQ
>Mature_403_residues
SPIEKSSKLDNVCYDIRGPVLKEAKRLEEEGHKVLKLNIGNPAPFGFDAPDEILVDVIRNLPTAQGYSDSKGLFSARKAI
MQHYQARDIRDVTVEDIYIGNGVSELIVQSMQALLNSGDEMLVPAPDYPLWTAAVSLSGGKAVHYLCDEEAGWFPDLDDI
ISKITPRTRGIVIINPNNPTGAVYSKALLEQIVEIARQHNLIIFSDEIYDKILYDEAEHHSIAALAPDLLTVTFNGLSKT
YRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPMQHAIQTALGGYQSINEFIQPGGRLYEQRNRTWELLNEIP
GVSCVKPQGALYMFPRIDAKRFNIHDDQKLVLDLLLQEKVLLVQGSAFNWPYPDHVRIVTLPRVDELEMAVGKLGRFLEG
YRQ

Specific function: Unknown

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4507369, Length=379, Percent_Identity=26.3852242744063, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI19263340, Length=470, Percent_Identity=25.9574468085106, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI215599424, Length=376, Percent_Identity=27.3936170212766, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI4885351, Length=436, Percent_Identity=25, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI95147551, Length=171, Percent_Identity=27.4853801169591, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI169881279, Length=171, Percent_Identity=27.4853801169591, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI56713256, Length=299, Percent_Identity=24.4147157190635, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI56713254, Length=299, Percent_Identity=24.4147157190635, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI169881281, Length=161, Percent_Identity=29.1925465838509, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI1788627, Length=404, Percent_Identity=89.3564356435644, Blast_Score=762, Evalue=0.0,
Organism=Escherichia coli, GI1788722, Length=379, Percent_Identity=27.1767810026385, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786816, Length=238, Percent_Identity=26.890756302521, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17567663, Length=375, Percent_Identity=28.2666666666667, Blast_Score=157, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI71981209, Length=464, Percent_Identity=25.2155172413793, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71994476, Length=184, Percent_Identity=32.6086956521739, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994472, Length=184, Percent_Identity=32.6086956521739, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71981215, Length=293, Percent_Identity=25.5972696245734, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17567369, Length=202, Percent_Identity=27.7227722772277, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320317, Length=360, Percent_Identity=28.8888888888889, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323118, Length=376, Percent_Identity=27.9255319148936, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322401, Length=305, Percent_Identity=24.2622950819672, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859735, Length=376, Percent_Identity=26.063829787234, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI45551451, Length=488, Percent_Identity=27.0491803278689, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24641770, Length=488, Percent_Identity=27.0491803278689, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24641760, Length=488, Percent_Identity=27.0491803278689, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24641768, Length=488, Percent_Identity=27.0491803278689, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24641766, Length=488, Percent_Identity=27.0491803278689, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24641764, Length=488, Percent_Identity=27.0491803278689, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28573069, Length=324, Percent_Identity=25.3086419753086, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24646114, Length=324, Percent_Identity=25.3086419753086, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28573067, Length=324, Percent_Identity=25.3086419753086, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28573065, Length=324, Percent_Identity=25.3086419753086, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001176
- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.1

Molecular weight: Translated: 45284; Mature: 45152

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIEKSSKLDNVCYDIRGPVLKEAKRLEEEGHKVLKLNIGNPAPFGFDAPDEILVDVIR
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPTAQGYSDSKGLFSARKAIMQHYQARDIRDVTVEDIYIGNGVSELIVQSMQALLNSGD
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
EMLVPAPDYPLWTAAVSLSGGKAVHYLCDEEAGWFPDLDDIISKITPRTRGIVIINPNNP
CEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCCC
TGAVYSKALLEQIVEIARQHNLIIFSDEIYDKILYDEAEHHSIAALAPDLLTVTFNGLSK
CHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCHH
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPMQHAIQTALGGYQSINEFI
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
QPGGRLYEQRNRTWELLNEIPGVSCVKPQGALYMFPRIDAKRFNIHDDQKLVLDLLLQEK
CCCHHHHHHHCHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
VLLVQGSAFNWPYPDHVRIVTLPRVDELEMAVGKLGRFLEGYRQ
EEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPIEKSSKLDNVCYDIRGPVLKEAKRLEEEGHKVLKLNIGNPAPFGFDAPDEILVDVIR
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
NLPTAQGYSDSKGLFSARKAIMQHYQARDIRDVTVEDIYIGNGVSELIVQSMQALLNSGD
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCC
EMLVPAPDYPLWTAAVSLSGGKAVHYLCDEEAGWFPDLDDIISKITPRTRGIVIINPNNP
CEEEECCCCCCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCCC
TGAVYSKALLEQIVEIARQHNLIIFSDEIYDKILYDEAEHHSIAALAPDLLTVTFNGLSK
CHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHCCCCCCCHHHHCCCEEEEEECCCHH
TYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRLCANVPMQHAIQTALGGYQSINEFI
HHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHH
QPGGRLYEQRNRTWELLNEIPGVSCVKPQGALYMFPRIDAKRFNIHDDQKLVLDLLLQEK
CCCHHHHHHHCHHHHHHHHCCCCEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCC
VLLVQGSAFNWPYPDHVRIVTLPRVDELEMAVGKLGRFLEGYRQ
EEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate; 2-oxoglutarate

Specific reaction: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]