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The map label for this gene is yafC [H]

Identifier: 157371532

GI number: 157371532

Start: 3647456

End: 3648331

Strand: Direct

Name: yafC [H]

Synonym: Spro_3295

Alternate gene names: 157371532

Gene position: 3647456-3648331 (Clockwise)

Preceding gene: 157371526

Following gene: 157371547

Centisome position: 66.94

GC content: 57.42

Gene sequence:

>876_bases
ATGATGAATTGGGATGACGCACGCTTCTTCCTGGCAGTAGCCCGCTGCGGCACTTTACGAAAGGCCGCCAATGAACTACA
CGTCGACCAGGCCACCGTCGGACGGCGTATTACCGCTTTTGAAGAGAGCCTGGGCTCCAAGCTGTTTATCCGCACGCCGA
AATCATTCTCCCTCAGCCCTTTGGGTGAGGAAATGCTGGCAGACGTGATGAATATGGAAAATGCCGTGCAGGCGATTGGC
CGCAAGGCCACCAGCGGCGACGACAGCCTGTGCGGTAACGTGCGCATCGCCACCACCGACACCATGGCCGAGGCCTTTGT
CATTCCGGCCTTGAAAGCGCTGCGGGAGCAACATCCGTTAATCACCGTCACTATGCTGACGGCGGTGAATATCTCTGATA
TTTCTTACCGAGGAGCGGATTTGGCGATCCGTGGCGCCCGGCCAGAGTCCGATGAGCTGATTATCAAACGTATGGCAACC
ATCGAAATGGGGCTGTATGCCACTCAGGACTATCTCGACAAACACGGACTGCCGACCAAGGGCGACCACCTGAGTGGCCA
TGACCTGCTGATGTTTCCACGCGAGCTGGTGCCGCGCCACTGGAACGACTTTTGTGGCGAAGCGCTGATCGACCCCAATG
TAGTTTTGCAGTGCAATTCACAGTTGCTGTTGCGCTCGGCAACGCGTAACGGATTGGGCATCGGCCTGCTGTCCTCCTTC
CTGGCGGACAAGGACCCGGATCTGGTGCGTATCTTCCCGGAGAATAAAGACTGGGTAGATATCTGGCTGGTACTGCACCC
CGACCTGCAGCGCGCCGCGCGGGTACGTGCCGTAGTGCAGGCGTTGGAAACCTCGTTTAAAAACAATCCCGGTTGA

Upstream 100 bases:

>100_bases
ATTATGATACCAGCCCTGACTTGTCGCCTAAACCCGTGCCCGTAAGGTTGCGCAAGAATGAACACCAACAAGGCCGATAA
GCCAGCATAAAGGAAATGGC

Downstream 100 bases:

>100_bases
TCGTTCCAGTACTGCATCACTTCCCCGTCCCCATAAAAAAAGGTCGCCGAAGCGACCTTTTTTACTGCCTTGATGCCAAT
CAGAACATCGGCTGTGCCAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MMNWDDARFFLAVARCGTLRKAANELHVDQATVGRRITAFEESLGSKLFIRTPKSFSLSPLGEEMLADVMNMENAVQAIG
RKATSGDDSLCGNVRIATTDTMAEAFVIPALKALREQHPLITVTMLTAVNISDISYRGADLAIRGARPESDELIIKRMAT
IEMGLYATQDYLDKHGLPTKGDHLSGHDLLMFPRELVPRHWNDFCGEALIDPNVVLQCNSQLLLRSATRNGLGIGLLSSF
LADKDPDLVRIFPENKDWVDIWLVLHPDLQRAARVRAVVQALETSFKNNPG

Sequences:

>Translated_291_residues
MMNWDDARFFLAVARCGTLRKAANELHVDQATVGRRITAFEESLGSKLFIRTPKSFSLSPLGEEMLADVMNMENAVQAIG
RKATSGDDSLCGNVRIATTDTMAEAFVIPALKALREQHPLITVTMLTAVNISDISYRGADLAIRGARPESDELIIKRMAT
IEMGLYATQDYLDKHGLPTKGDHLSGHDLLMFPRELVPRHWNDFCGEALIDPNVVLQCNSQLLLRSATRNGLGIGLLSSF
LADKDPDLVRIFPENKDWVDIWLVLHPDLQRAARVRAVVQALETSFKNNPG
>Mature_291_residues
MMNWDDARFFLAVARCGTLRKAANELHVDQATVGRRITAFEESLGSKLFIRTPKSFSLSPLGEEMLADVMNMENAVQAIG
RKATSGDDSLCGNVRIATTDTMAEAFVIPALKALREQHPLITVTMLTAVNISDISYRGADLAIRGARPESDELIIKRMAT
IEMGLYATQDYLDKHGLPTKGDHLSGHDLLMFPRELVPRHWNDFCGEALIDPNVVLQCNSQLLLRSATRNGLGIGLLSSF
LADKDPDLVRIFPENKDWVDIWLVLHPDLQRAARVRAVVQALETSFKNNPG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=24.5733788395904, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1789639, Length=183, Percent_Identity=24.0437158469945, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI157672245, Length=216, Percent_Identity=28.7037037037037, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI145693193, Length=193, Percent_Identity=27.4611398963731, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1787128, Length=241, Percent_Identity=25.7261410788382, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787589, Length=220, Percent_Identity=23.6363636363636, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=25.4295532646048, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=24.2105263157895, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790208, Length=152, Percent_Identity=28.9473684210526, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32254; Mature: 32254

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNWDDARFFLAVARCGTLRKAANELHVDQATVGRRITAFEESLGSKLFIRTPKSFSLSP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCEEEEECCCCCCCCC
LGEEMLADVMNMENAVQAIGRKATSGDDSLCGNVRIATTDTMAEAFVIPALKALREQHPL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCE
ITVTMLTAVNISDISYRGADLAIRGARPESDELIIKRMATIEMGLYATQDYLDKHGLPTK
EEEEEEEEEEECCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDHLSGHDLLMFPRELVPRHWNDFCGEALIDPNVVLQCNSQLLLRSATRNGLGIGLLSSF
CCCCCCCCCEECCHHHCCCHHHHHHCHHHCCCCEEEEECCHHHHHHHCCCCCHHHHHHHH
LADKDPDLVRIFPENKDWVDIWLVLHPDLQRAARVRAVVQALETSFKNNPG
HCCCCCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMNWDDARFFLAVARCGTLRKAANELHVDQATVGRRITAFEESLGSKLFIRTPKSFSLSP
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCEEEEECCCCCCCCC
LGEEMLADVMNMENAVQAIGRKATSGDDSLCGNVRIATTDTMAEAFVIPALKALREQHPL
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCE
ITVTMLTAVNISDISYRGADLAIRGARPESDELIIKRMATIEMGLYATQDYLDKHGLPTK
EEEEEEEEEEECCCEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GDHLSGHDLLMFPRELVPRHWNDFCGEALIDPNVVLQCNSQLLLRSATRNGLGIGLLSSF
CCCCCCCCCEECCHHHCCCHHHHHHCHHHCCCCEEEEECCHHHHHHHCCCCCHHHHHHHH
LADKDPDLVRIFPENKDWVDIWLVLHPDLQRAARVRAVVQALETSFKNNPG
HCCCCCCEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]