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The map label for this gene is setB [H]

Identifier: 157371471

GI number: 157371471

Start: 3567077

End: 3568306

Strand: Direct

Name: setB [H]

Synonym: Spro_3234

Alternate gene names: 157371471

Gene position: 3567077-3568306 (Clockwise)

Preceding gene: 157371470

Following gene: 157371473

Centisome position: 65.46

GC content: 57.72

Gene sequence:

>1230_bases
ATGGGCGTATTATGCGCCGGATCCCTCATTGACGGCTATTTTCTGACCTCTATGTCTACATCATCTGCGGCAACGCGACG
CTCGCCCGACCTCACTTCACTGGCTTTTTTAGTGGTGGCGTTTCTCACCGGTATTGCCGGGGCGCTACAGACCCCTACGT
TGAGCCTGTTCCTGGCGACGGAAGTGAAGGTGCGCCCCTCGATGGTTGGGCTGTTTTTCACCGGTAGCGCGGTGATTGGC
ATTCTGGTCAGCCAGTTTCTCGCTCGCCGTTCGGATCAAAAAGGCGATCGCAAAACGCTGATTTTCCTCTGCTGCATGCT
GGGTGCGCTGGGCTGCGTGCTGTTCGCCTGGAACCGCAATTATTACCTGTTACTGGCGGTCGGCGTGATGTTGACCAGCT
TCGGTTCCACCGCTAACCCGCAGATGTTCGCCCTGGCGCGCGAACATGCCGATCGCACCGGTCGCGAAGCCGTCATGTTC
AGTTCAATCCTGCGGGCGCAGGTTTCCCTCGCCTGGGTCATAGGGCCACCCATCGCCTTTGCACTGGCCCTGGGATTCGG
TTTTAAAGTGATGTATCTGGCCGCCGCGGCGGCGTTCATCCTGTGTGGCGTGCTGGTGTGGAAAATGCTGCCCTCGATGC
GCAAAACACCAATGGCGGCCAGCACCACACTGGAGGCCCCACGACAAAACCGTCGCGATACGCTGCTGCTGTTTATGGCC
TGCACCTTTATGTGGACGGCCAACAGCATGTATCTGATCAATATGCCGCTGTATATGGTGCATGAGCTGCATCTGCCGGA
AAGGCTGGCCGGGATATTAATGGGTACGGCGGCCGGGCTGGAGATACCGACCATGCTGCTTGCCGGCATGGTGGCGAAAC
GCTTCGGCAAGCGCTTTTTAATGCGTTGGGCGGTGGTGGCGGGCCTGCTGTTTTATGTTGGCCTGCTGTTTATCACCGGC
ACCTGGCAACTGATCGCCCTGCAACTGCTTAACGCGGCGTTTATCGGCGTACTCGCGGGGATCGGTATGCTCTATTTTCA
GGATTTGATGCCTGGACAGGCCGGTGCCGCAACCACCCTGTTTACCAACACCACTCGCGTGGGTTGGATTTTAGCCGGTT
CCATCGCTGGCGTAGTGGCGGAAGTCTGGAATTATCACGCGGTGTTTTATTCCGCACTGGCGATGGTTGTGGGCGCAGTG
CTGTGCTTGTGGAAGCTTAAAGACGCGTAA

Upstream 100 bases:

>100_bases
ATGGTGACATCGCCCTTTTTTATGCCTTTATACCGGAAAAAAACTTTACCAGTAAAAGGAATGAAACCGGTTTCGGTTCA
TTTCTTTTTTTTACAAATAA

Downstream 100 bases:

>100_bases
AACAGGCTTAGGGGGCCGTATCCGCTTCCAGCTTGGCCAAATAGATTAGCGCCTGGTCTCGGTGGTCGTGGCACATTTCG
CGGCTGGCCTGCAGGCTGCC

Product: sugar efflux transporter

Products: lactose [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MGVLCAGSLIDGYFLTSMSTSSAATRRSPDLTSLAFLVVAFLTGIAGALQTPTLSLFLATEVKVRPSMVGLFFTGSAVIG
ILVSQFLARRSDQKGDRKTLIFLCCMLGALGCVLFAWNRNYYLLLAVGVMLTSFGSTANPQMFALAREHADRTGREAVMF
SSILRAQVSLAWVIGPPIAFALALGFGFKVMYLAAAAAFILCGVLVWKMLPSMRKTPMAASTTLEAPRQNRRDTLLLFMA
CTFMWTANSMYLINMPLYMVHELHLPERLAGILMGTAAGLEIPTMLLAGMVAKRFGKRFLMRWAVVAGLLFYVGLLFITG
TWQLIALQLLNAAFIGVLAGIGMLYFQDLMPGQAGAATTLFTNTTRVGWILAGSIAGVVAEVWNYHAVFYSALAMVVGAV
LCLWKLKDA

Sequences:

>Translated_409_residues
MGVLCAGSLIDGYFLTSMSTSSAATRRSPDLTSLAFLVVAFLTGIAGALQTPTLSLFLATEVKVRPSMVGLFFTGSAVIG
ILVSQFLARRSDQKGDRKTLIFLCCMLGALGCVLFAWNRNYYLLLAVGVMLTSFGSTANPQMFALAREHADRTGREAVMF
SSILRAQVSLAWVIGPPIAFALALGFGFKVMYLAAAAAFILCGVLVWKMLPSMRKTPMAASTTLEAPRQNRRDTLLLFMA
CTFMWTANSMYLINMPLYMVHELHLPERLAGILMGTAAGLEIPTMLLAGMVAKRFGKRFLMRWAVVAGLLFYVGLLFITG
TWQLIALQLLNAAFIGVLAGIGMLYFQDLMPGQAGAATTLFTNTTRVGWILAGSIAGVVAEVWNYHAVFYSALAMVVGAV
LCLWKLKDA
>Mature_408_residues
GVLCAGSLIDGYFLTSMSTSSAATRRSPDLTSLAFLVVAFLTGIAGALQTPTLSLFLATEVKVRPSMVGLFFTGSAVIGI
LVSQFLARRSDQKGDRKTLIFLCCMLGALGCVLFAWNRNYYLLLAVGVMLTSFGSTANPQMFALAREHADRTGREAVMFS
SILRAQVSLAWVIGPPIAFALALGFGFKVMYLAAAAAFILCGVLVWKMLPSMRKTPMAASTTLEAPRQNRRDTLLLFMAC
TFMWTANSMYLINMPLYMVHELHLPERLAGILMGTAAGLEIPTMLLAGMVAKRFGKRFLMRWAVVAGLLFYVGLLFITGT
WQLIALQLLNAAFIGVLAGIGMLYFQDLMPGQAGAATTLFTNTTRVGWILAGSIAGVVAEVWNYHAVFYSALAMVVGAVL
CLWKLKDA

Specific function: Involved in the efflux of sugars. The physiological role may be the detoxification of non-metabolizable sugar analogs. Can transport lactose and glucose [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Set transporter family [H]

Homologues:

Organism=Escherichia coli, GI1788495, Length=390, Percent_Identity=71.2820512820513, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1790091, Length=393, Percent_Identity=61.323155216285, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI48994875, Length=376, Percent_Identity=54.5212765957447, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004750 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44263; Mature: 44131

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVLCAGSLIDGYFLTSMSTSSAATRRSPDLTSLAFLVVAFLTGIAGALQTPTLSLFLAT
CCEEEECHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
EVKVRPSMVGLFFTGSAVIGILVSQFLARRSDQKGDRKTLIFLCCMLGALGCVLFAWNRN
EEEECCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
YYLLLAVGVMLTSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPIAF
EEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHH
ALALGFGFKVMYLAAAAAFILCGVLVWKMLPSMRKTPMAASTTLEAPRQNRRDTLLLFMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHH
CTFMWTANSMYLINMPLYMVHELHLPERLAGILMGTAAGLEIPTMLLAGMVAKRFGKRFL
HHHHHHCCCEEEEECHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MRWAVVAGLLFYVGLLFITGTWQLIALQLLNAAFIGVLAGIGMLYFQDLMPGQAGAATTL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
FTNTTRVGWILAGSIAGVVAEVWNYHAVFYSALAMVVGAVLCLWKLKDA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GVLCAGSLIDGYFLTSMSTSSAATRRSPDLTSLAFLVVAFLTGIAGALQTPTLSLFLAT
CEEEECHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
EVKVRPSMVGLFFTGSAVIGILVSQFLARRSDQKGDRKTLIFLCCMLGALGCVLFAWNRN
EEEECCHHEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
YYLLLAVGVMLTSFGSTANPQMFALAREHADRTGREAVMFSSILRAQVSLAWVIGPPIAF
EEEHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECHHHHH
ALALGFGFKVMYLAAAAAFILCGVLVWKMLPSMRKTPMAASTTLEAPRQNRRDTLLLFMA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHHHHHHHHH
CTFMWTANSMYLINMPLYMVHELHLPERLAGILMGTAAGLEIPTMLLAGMVAKRFGKRFL
HHHHHHCCCEEEEECHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
MRWAVVAGLLFYVGLLFITGTWQLIALQLLNAAFIGVLAGIGMLYFQDLMPGQAGAATTL
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
FTNTTRVGWILAGSIAGVVAEVWNYHAVFYSALAMVVGAVLCLWKLKDA
EECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lactose [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: lactose [Cytoplasm] + Proton [Periplasm] = lactose [Periplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 10209755; 10438463 [H]