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The map label for this gene is nfo

Identifier: 157371464

GI number: 157371464

Start: 3557516

End: 3558355

Strand: Direct

Name: nfo

Synonym: Spro_3227

Alternate gene names: 157371464

Gene position: 3557516-3558355 (Clockwise)

Preceding gene: 157371463

Following gene: 157371468

Centisome position: 65.29

GC content: 55.95

Gene sequence:

>840_bases
ATGAAGTTTGTCGGTGCACATGTCAGCGCGTCAGGCGGCGTGGATCAGGCGGTTATCCGCGCGCACGAATTAGAGGCGAC
CGCATTCGCCCTGTTCACTAAAAATCAGCGTCAATGGAAGGCCGCCCCGCTGCCTGCCGATGTGATTGATAAATTCAAGA
GCGCCTGCGCGCAGTACGGTTACGGCCCAGGACAGATCCTGCCGCACGACAGCTACCTGATTAATCTCGGCCACCCGGTG
ACCGAGGCATTGGAAAAATCCCGCGAAGCCTTTCTTGATGAGATGCAGCGTTGTGAGCAACTGGGGCTGACGCTGCTGAA
CTTCCATCCTGGCAGCCATCTGATGCAAATTGACGAAGACAAATGCCTGGCACGCATCGCTGAATCCATCAACCTGGTGC
TGGATAAAACCAGCGGTGTGACAGCGGTGATCGAAAATACCGCCGGTCAGGGCAGTAATCTGGGCTTTAAGTTTGAACAT
TTGGCGGCGATCATCGACGGCGTGGAAGACAAAAGCCGCGTCGGCGTCTGCATCGACACCTGTCACGCTTTTGCCGCCGG
TTACGATCTGCGTACCGAGGAAACCTGCGAACAGACCTTTAAGGAACTCGGTGAGGTGGTGGGCTTCAACTATTTGCGCG
GTATGCACCTGAACGACGCCAAAAGCGAATTCGGCAGTCGCGTTGACCGTCATCACAGCCTAGGTGAGGGCAACATCGGT
AAAACGGTGTTCAGCTACATCATGCGCGACCCGCGCTTCGACAAAATCCCGCTGATCCTGGAAACGGTGAACCCGGATAT
CTGGGCGGAAGAGATCGCCTGGCTAAAAGCCCAGCAGTAG

Upstream 100 bases:

>100_bases
CCACTACCTCAGGCCCGCCATCGCGGGCCTGAACTTTTTCAGCCATTCAATCCTTCCGCTATCAATGCCATAATGACGCC
GATTACACAGGAGAATGAAA

Downstream 100 bases:

>100_bases
TTGTCGCAATAAAAAACCGGAGCCTGGACTCTAATGCCGGTCAGTTAAGCAAAGTTCTGAATAAGAATGTACGACGTTGT
TTAAGTGGGCGTCCCCCTCT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKFVGAHVSASGGVDQAVIRAHELEATAFALFTKNQRQWKAAPLPADVIDKFKSACAQYGYGPGQILPHDSYLINLGHPV
TEALEKSREAFLDEMQRCEQLGLTLLNFHPGSHLMQIDEDKCLARIAESINLVLDKTSGVTAVIENTAGQGSNLGFKFEH
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTEETCEQTFKELGEVVGFNYLRGMHLNDAKSEFGSRVDRHHSLGEGNIG
KTVFSYIMRDPRFDKIPLILETVNPDIWAEEIAWLKAQQ

Sequences:

>Translated_279_residues
MKFVGAHVSASGGVDQAVIRAHELEATAFALFTKNQRQWKAAPLPADVIDKFKSACAQYGYGPGQILPHDSYLINLGHPV
TEALEKSREAFLDEMQRCEQLGLTLLNFHPGSHLMQIDEDKCLARIAESINLVLDKTSGVTAVIENTAGQGSNLGFKFEH
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTEETCEQTFKELGEVVGFNYLRGMHLNDAKSEFGSRVDRHHSLGEGNIG
KTVFSYIMRDPRFDKIPLILETVNPDIWAEEIAWLKAQQ
>Mature_279_residues
MKFVGAHVSASGGVDQAVIRAHELEATAFALFTKNQRQWKAAPLPADVIDKFKSACAQYGYGPGQILPHDSYLINLGHPV
TEALEKSREAFLDEMQRCEQLGLTLLNFHPGSHLMQIDEDKCLARIAESINLVLDKTSGVTAVIENTAGQGSNLGFKFEH
LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTEETCEQTFKELGEVVGFNYLRGMHLNDAKSEFGSRVDRHHSLGEGNIG
KTVFSYIMRDPRFDKIPLILETVNPDIWAEEIAWLKAQQ

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=279, Percent_Identity=81.0035842293907, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17531193, Length=261, Percent_Identity=49.4252873563218, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6322735, Length=280, Percent_Identity=43.2142857142857, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_SERP5 (A8GGT5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479453.1
- ProteinModelPortal:   A8GGT5
- SMR:   A8GGT5
- STRING:   A8GGT5
- GeneID:   5606284
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3227
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   SPRO399741:SPRO_3227-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30862; Mature: 30862

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVGAHVSASGGVDQAVIRAHELEATAFALFTKNQRQWKAAPLPADVIDKFKSACAQYG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
YGPGQILPHDSYLINLGHPVTEALEKSREAFLDEMQRCEQLGLTLLNFHPGSHLMQIDED
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEECHH
KCLARIAESINLVLDKTSGVTAVIENTAGQGSNLGFKFEHLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRTEETCEQTFKELGEVVGFNYLRGMHLNDAKSEFGSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
KTVFSYIMRDPRFDKIPLILETVNPDIWAEEIAWLKAQQ
HHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKFVGAHVSASGGVDQAVIRAHELEATAFALFTKNQRQWKAAPLPADVIDKFKSACAQYG
CCEECCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHCC
YGPGQILPHDSYLINLGHPVTEALEKSREAFLDEMQRCEQLGLTLLNFHPGSHLMQIDED
CCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEECHH
KCLARIAESINLVLDKTSGVTAVIENTAGQGSNLGFKFEHLAAIIDGVEDKSRVGVCIDT
HHHHHHHHHHHEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHH
CHAFAAGYDLRTEETCEQTFKELGEVVGFNYLRGMHLNDAKSEFGSRVDRHHSLGEGNIG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHH
KTVFSYIMRDPRFDKIPLILETVNPDIWAEEIAWLKAQQ
HHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA