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The map label for this gene is nepI [H]

Identifier: 157371377

GI number: 157371377

Start: 3453445

End: 3454647

Strand: Direct

Name: nepI [H]

Synonym: Spro_3138

Alternate gene names: 157371377

Gene position: 3453445-3454647 (Clockwise)

Preceding gene: 157371374

Following gene: 157371381

Centisome position: 63.38

GC content: 54.61

Gene sequence:

>1203_bases
ATGCATACGACGACTACATCGGCTTCAAGCAGGAACACGGAAACCCTGCCTATCGCGGCACTGCTTGCCCTAGCGATGGC
AGCCTTTATCACGCTGTTGACCGAAATCATGCCGGCAGGATTGCTTTCCTCCATCGCGGGGGGTTTGAATGTATCCGAAA
GTTTGGCCGGTCAGTTCATCACTGCCTACGCGGTGGGGGCACTGGTCGCTGCTATTCCAGTGACAACCCTTACGCAGGGT
ATGCGGCGGCGTCCTTTGCTGTTGGTGGCGATCTTTGGGTTTTCGCTTGTCAATCTCGTCACCGCGCTTTCCGATAGCTA
TAGCATCTCATTGGTAGCGCGCTTCTTTGCCGGTGTCTTCGGTGGCATTGTTTGGTCGCTGCTAGCCGGCTTTGCTGTAC
GCATCTCACCATCTCATCTCAGCGGGCGGGCGATTGCGATATCCGGCGCTGGCGCGACCATAGCTTTGGTGTTCGGCGTA
CCGCTCGGCGCGTTGCTTGGTCGAACCATTGGCTGGCAAGGTGCTTTTGGTCTGATGACTGTCCTGGCACTGGCGCTCAT
CGTATGGGTTATCGCCGTCGTTCCGGATTTTCCCGGTCAGACCAAGGAGCAGCGCCAGCCTCTTACAGGAGTCTTTCTAA
AGACTGGCATTCGTGCCGTACTGTTTGTGGTCTTTACCTTCGTTGTCGCTCACAACATTCTCTATATCTACATTGAGCCC
CTCCTCGAACCCTCGGGCTTGTCCGCGGATGTAGATATTGTTCTGTTCGTCTTCGGAATAGGCTCAATCATTGGCCTGTG
GTTCGTCGGCTCAATGGTTGATCGCCGACTGCAACTCCTTGCTGTTGCAAGCATCGTGGTCTTCGCTCTTGCTTCCCTGC
TACTTGCTTTTTGGGGTGGCCTTCCAAAGATGGTTTATTCTTCCGTCGTGATCTGGGGAATGGCCGTGGGTGGATTTGCT
ACGATCACCCAAACGGCATTGTCTCGTTTTGCCGGTGAATCGGTCGATGTCGCGCAATCTATGTATACGACTGGTTGGAA
CAGCGCCGTTGCCGCCGGCGGGATTGTCGGAGGTATTTTGCTGGATCGAACAGGCTTGGCGTCCTTTGCATGGGGTGTTA
TCGGACTTCTACTCCTCTCGTTGTTAGGCACGATTCTCGCAATGAACAGGGCGTTATCGCAAAAGGCCGAAAACACGATG
TGA

Upstream 100 bases:

>100_bases
TTGTGAATTATTGCCTATTTTTCTTCTTTTCGCCAACGAGCACACTGTCGGGTAGCGCTCTGCAAAGGGCTGAGACAACT
TCGATTTGAGGAATTTTTTG

Downstream 100 bases:

>100_bases
GGAAATGTTTGGGCGCACAGCCCCCTCTTATCCGGCTCCTTAATTTCTCAGTCGATACTGTAAAAACCACAGACCATTTC
CCAATGACAATGATCTGTGG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MHTTTTSASSRNTETLPIAALLALAMAAFITLLTEIMPAGLLSSIAGGLNVSESLAGQFITAYAVGALVAAIPVTTLTQG
MRRRPLLLVAIFGFSLVNLVTALSDSYSISLVARFFAGVFGGIVWSLLAGFAVRISPSHLSGRAIAISGAGATIALVFGV
PLGALLGRTIGWQGAFGLMTVLALALIVWVIAVVPDFPGQTKEQRQPLTGVFLKTGIRAVLFVVFTFVVAHNILYIYIEP
LLEPSGLSADVDIVLFVFGIGSIIGLWFVGSMVDRRLQLLAVASIVVFALASLLLAFWGGLPKMVYSSVVIWGMAVGGFA
TITQTALSRFAGESVDVAQSMYTTGWNSAVAAGGIVGGILLDRTGLASFAWGVIGLLLLSLLGTILAMNRALSQKAENTM

Sequences:

>Translated_400_residues
MHTTTTSASSRNTETLPIAALLALAMAAFITLLTEIMPAGLLSSIAGGLNVSESLAGQFITAYAVGALVAAIPVTTLTQG
MRRRPLLLVAIFGFSLVNLVTALSDSYSISLVARFFAGVFGGIVWSLLAGFAVRISPSHLSGRAIAISGAGATIALVFGV
PLGALLGRTIGWQGAFGLMTVLALALIVWVIAVVPDFPGQTKEQRQPLTGVFLKTGIRAVLFVVFTFVVAHNILYIYIEP
LLEPSGLSADVDIVLFVFGIGSIIGLWFVGSMVDRRLQLLAVASIVVFALASLLLAFWGGLPKMVYSSVVIWGMAVGGFA
TITQTALSRFAGESVDVAQSMYTTGWNSAVAAGGIVGGILLDRTGLASFAWGVIGLLLLSLLGTILAMNRALSQKAENTM
>Mature_400_residues
MHTTTTSASSRNTETLPIAALLALAMAAFITLLTEIMPAGLLSSIAGGLNVSESLAGQFITAYAVGALVAAIPVTTLTQG
MRRRPLLLVAIFGFSLVNLVTALSDSYSISLVARFFAGVFGGIVWSLLAGFAVRISPSHLSGRAIAISGAGATIALVFGV
PLGALLGRTIGWQGAFGLMTVLALALIVWVIAVVPDFPGQTKEQRQPLTGVFLKTGIRAVLFVVFTFVVAHNILYIYIEP
LLEPSGLSADVDIVLFVFGIGSIIGLWFVGSMVDRRLQLLAVASIVVFALASLLLAFWGGLPKMVYSSVVIWGMAVGGFA
TITQTALSRFAGESVDVAQSMYTTGWNSAVAAGGIVGGILLDRTGLASFAWGVIGLLLLSLLGTILAMNRALSQKAENTM

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and especially inosine. Involved in the resistance to 6-mercaptopurine [H]

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. NepI (TC 2.A.1.2.26) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=353, Percent_Identity=27.7620396600567, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1787808, Length=247, Percent_Identity=29.1497975708502, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787947, Length=322, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41682; Mature: 41682

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTTTTSASSRNTETLPIAALLALAMAAFITLLTEIMPAGLLSSIAGGLNVSESLAGQFI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TAYAVGALVAAIPVTTLTQGMRRRPLLLVAIFGFSLVNLVTALSDSYSISLVARFFAGVF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GGIVWSLLAGFAVRISPSHLSGRAIAISGAGATIALVFGVPLGALLGRTIGWQGAFGLMT
HHHHHHHHHCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VLALALIVWVIAVVPDFPGQTKEQRQPLTGVFLKTGIRAVLFVVFTFVVAHNILYIYIEP
HHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
LLEPSGLSADVDIVLFVFGIGSIIGLWFVGSMVDRRLQLLAVASIVVFALASLLLAFWGG
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPKMVYSSVVIWGMAVGGFATITQTALSRFAGESVDVAQSMYTTGWNSAVAAGGIVGGIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
LDRTGLASFAWGVIGLLLLSLLGTILAMNRALSQKAENTM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHTTTTSASSRNTETLPIAALLALAMAAFITLLTEIMPAGLLSSIAGGLNVSESLAGQFI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
TAYAVGALVAAIPVTTLTQGMRRRPLLLVAIFGFSLVNLVTALSDSYSISLVARFFAGVF
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GGIVWSLLAGFAVRISPSHLSGRAIAISGAGATIALVFGVPLGALLGRTIGWQGAFGLMT
HHHHHHHHHCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VLALALIVWVIAVVPDFPGQTKEQRQPLTGVFLKTGIRAVLFVVFTFVVAHNILYIYIEP
HHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
LLEPSGLSADVDIVLFVFGIGSIIGLWFVGSMVDRRLQLLAVASIVVFALASLLLAFWGG
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPKMVYSSVVIWGMAVGGFATITQTALSRFAGESVDVAQSMYTTGWNSAVAAGGIVGGIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
LDRTGLASFAWGVIGLLLLSLLGTILAMNRALSQKAENTM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA