BacMap

Definition	Serratia proteamaculans 568 chromosome, complete genome.
Accession	NC_009832
Length	5,448,853

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The map label for this gene is paaG [H]

Identifier: 157371318

GI number: 157371318

Start: 3387401

End: 3388192

Strand: Direct

Name: paaG [H]

Synonym: Spro_3078

Alternate gene names: 157371318

Gene position: 3387401-3388192 (Clockwise)

Preceding gene: 157371317

Following gene: 157371319

Centisome position: 62.17

GC content: 62.12

Gene sequence:

>792_bases
ATGGATAACGCATTGATTCTCACCCATCTGGAAGCCGGGGTGTTGACCCTGACTCTTAACCGGCCAGACCGGCTCAACAG
TTTTAACGACGAGATGCACCGCCAGCTTAGCGAAGCGCTGACCCTCGCCGAGCGCGACGAAACCGTGCGCTGCCTGCTGA
TCACCGGTGCCGGGCGTGGTTTTTGCGCCGGACAGGATCTGAACGATCGCAACGTTAGCGTTGAGCAACAGGCGCCAGAT
CTCGGCCTGTCGGTGGAACGTTTCTACAACCCGCTGATCCGCCGCCTGACCGCCTTGCCAAAACCGGTGGTATGCGCCGT
CAACGGCGTGGCCGCGGGGGCCGGAGCCGCTTTGGCGCTGGCTTGCGACATCGTCATTGCCGCCGATAACGCCAACTTTA
TCCAGTCCTTCTGCCGTCTGGGCCTGGTACCGGACTCTGGCGGCAGTTGGTTCCTGCCACGCCTGGCAGGCCACGCCCGT
GCCATGGGGATGGCGCTGCTGGGTGACAAAGTCAGCGCACAGCAAGCGCTGGCCTGGGGCATGATCTGGCAGGTGGTTGC
TGCGGATGAACTGGCCGCCACCAGCCAAACGCTGGCGCAACACCTGGCTACTCAGCCAACCTATGGGCTGGGCCTGATTA
AAAAGGCCCTCTACAGTTCGGCCAACAACAGCCTTGACCAACAGTTGGATCTGGAACGCGATCTGCAGCGTCTGGGCGGT
CGCAGCGACGACTACCGCGAGGGCGTCAGCGCCTTCTTCGCCAAACGCGCGCCCAATTTCAGCGGGAAATAA

Upstream 100 bases:

>100_bases
GCGCCAGCAGTTTGTCACCCTGGCCGCCACCGACGATCGCCGTGAAGGCATCGCCGCCTTCTTCGAAAAACGTACGCCAA
ACTATCAGGGGCGCTGATTT

Downstream 100 bases:

>100_bases
CCATGAACCAGCCGAACTTAAACCTCAGGGTGGCGGTGATTGGTGCCGGTACCATGGGTATCGGTATTGCTCAGGTGGCC
GCCGCCGCCGGGCATCAGGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MDNALILTHLEAGVLTLTLNRPDRLNSFNDEMHRQLSEALTLAERDETVRCLLITGAGRGFCAGQDLNDRNVSVEQQAPD
LGLSVERFYNPLIRRLTALPKPVVCAVNGVAAGAGAALALACDIVIAADNANFIQSFCRLGLVPDSGGSWFLPRLAGHAR
AMGMALLGDKVSAQQALAWGMIWQVVAADELAATSQTLAQHLATQPTYGLGLIKKALYSSANNSLDQQLDLERDLQRLGG
RSDDYREGVSAFFAKRAPNFSGK

Sequences:

>Translated_263_residues
MDNALILTHLEAGVLTLTLNRPDRLNSFNDEMHRQLSEALTLAERDETVRCLLITGAGRGFCAGQDLNDRNVSVEQQAPD
LGLSVERFYNPLIRRLTALPKPVVCAVNGVAAGAGAALALACDIVIAADNANFIQSFCRLGLVPDSGGSWFLPRLAGHAR
AMGMALLGDKVSAQQALAWGMIWQVVAADELAATSQTLAQHLATQPTYGLGLIKKALYSSANNSLDQQLDLERDLQRLGG
RSDDYREGVSAFFAKRAPNFSGK
>Mature_263_residues
MDNALILTHLEAGVLTLTLNRPDRLNSFNDEMHRQLSEALTLAERDETVRCLLITGAGRGFCAGQDLNDRNVSVEQQAPD
LGLSVERFYNPLIRRLTALPKPVVCAVNGVAAGAGAALALACDIVIAADNANFIQSFCRLGLVPDSGGSWFLPRLAGHAR
AMGMALLGDKVSAQQALAWGMIWQVVAADELAATSQTLAQHLATQPTYGLGLIKKALYSSANNSLDQQLDLERDLQRLGG
RSDDYREGVSAFFAKRAPNFSGK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=31.7269076305221, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI45643119, Length=260, Percent_Identity=27.6923076923077, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI260275230, Length=260, Percent_Identity=27.6923076923077, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI260274832, Length=259, Percent_Identity=28.1853281853282, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=27.0916334661355, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI70995211, Length=196, Percent_Identity=31.1224489795918, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4757968, Length=189, Percent_Identity=31.2169312169312, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI49355787, Length=189, Percent_Identity=31.2169312169312, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221307494, Length=168, Percent_Identity=31.547619047619, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI221136756, Length=168, Percent_Identity=31.547619047619, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI221136753, Length=168, Percent_Identity=31.547619047619, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI25453481, Length=189, Percent_Identity=30.6878306878307, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI51538573, Length=189, Percent_Identity=30.6878306878307, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI4757966, Length=189, Percent_Identity=30.6878306878307, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI51538581, Length=189, Percent_Identity=30.6878306878307, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI213417737, Length=227, Percent_Identity=29.0748898678414, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=29.0748898678414, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI4502327, Length=270, Percent_Identity=28.8888888888889, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI68989263, Length=215, Percent_Identity=30.6976744186047, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI37594471, Length=227, Percent_Identity=26.8722466960352, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI37594469, Length=185, Percent_Identity=30.2702702702703, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI116235487, Length=168, Percent_Identity=29.7619047619048, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI20127408, Length=191, Percent_Identity=27.7486910994764, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787660, Length=259, Percent_Identity=71.8146718146718, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1787659, Length=250, Percent_Identity=38.4, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI221142681, Length=268, Percent_Identity=32.8358208955224, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1788597, Length=253, Percent_Identity=30.0395256916996, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=26.5625, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790281, Length=200, Percent_Identity=30, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788682, Length=229, Percent_Identity=29.6943231441048, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=35.0806451612903, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=31.6205533596838, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17535521, Length=250, Percent_Identity=28, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17549921, Length=189, Percent_Identity=31.7460317460317, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560910, Length=231, Percent_Identity=27.7056277056277, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=29.004329004329, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=257, Percent_Identity=25.2918287937743, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=27.3913043478261, Blast_Score=80, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=200, Percent_Identity=29, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=29.2929292929293, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=254, Percent_Identity=26.3779527559055, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=181, Percent_Identity=25.9668508287293, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=24.1758241758242, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6320241, Length=230, Percent_Identity=23.9130434782609, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI20129971, Length=248, Percent_Identity=32.6612903225806, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI24653477, Length=248, Percent_Identity=32.6612903225806, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=26.5625, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=29.6875, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24654903, Length=211, Percent_Identity=27.9620853080569, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24650670, Length=217, Percent_Identity=28.110599078341, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357171, Length=216, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920382, Length=253, Percent_Identity=26.8774703557312, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28571729, Length=145, Percent_Identity=33.7931034482759, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28571730, Length=145, Percent_Identity=33.7931034482759, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28250; Mature: 28250

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNALILTHLEAGVLTLTLNRPDRLNSFNDEMHRQLSEALTLAERDETVRCLLITGAGRG
CCCCEEEEEECCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FCAGQDLNDRNVSVEQQAPDLGLSVERFYNPLIRRLTALPKPVVCAVNGVAAGAGAALAL
EECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
ACDIVIAADNANFIQSFCRLGLVPDSGGSWFLPRLAGHARAMGMALLGDKVSAQQALAWG
HEEEEEEECCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MIWQVVAADELAATSQTLAQHLATQPTYGLGLIKKALYSSANNSLDQQLDLERDLQRLGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
RSDDYREGVSAFFAKRAPNFSGK
CCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDNALILTHLEAGVLTLTLNRPDRLNSFNDEMHRQLSEALTLAERDETVRCLLITGAGRG
CCCCEEEEEECCCEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCC
FCAGQDLNDRNVSVEQQAPDLGLSVERFYNPLIRRLTALPKPVVCAVNGVAAGAGAALAL
EECCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
ACDIVIAADNANFIQSFCRLGLVPDSGGSWFLPRLAGHARAMGMALLGDKVSAQQALAWG
HEEEEEEECCCHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MIWQVVAADELAATSQTLAQHLATQPTYGLGLIKKALYSSANNSLDQQLDLERDLQRLGG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
RSDDYREGVSAFFAKRAPNFSGK
CCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]