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The map label for this gene is outO [H]

Identifier: 157371142

GI number: 157371142

Start: 3190133

End: 3190834

Strand: Direct

Name: outO [H]

Synonym: Spro_2902

Alternate gene names: 157371142

Gene position: 3190133-3190834 (Clockwise)

Preceding gene: 157371140

Following gene: 157371143

Centisome position: 58.55

GC content: 59.4

Gene sequence:

>702_bases
ATGTTCGATAATCCTCAGCTCGATTTCTGGTTTCGCGGCCTGTTTATCCCGGCCATGTTACTGCCCGCAGCGCTCGCCAC
CAGACTGATCGGCATCCTCTCACCAGGCTTGACGCAACACCCGCTACCGAGGGAACGCACGCTAATGTTGATTGCCGTCA
GTGCCGGGCTGGCGGCCCTGCCTTTCAATATTCCACTGCTACAACGGCTATTGCTGCTGCCGGCAGCGGCGATATTACTG
GCGCTGGCTCTCATTGACTGGCAACAGCGACTATTACCGGATCGCCTGACCCAACCGCTGCTATGGGCCGGGCTGTTGGT
CAATCAGCAAGGCTATTTTGTTTCGCTGACCGAAGCGGTGACCGGGGCCTGCATCGGTTATCTTTCGCTGTGGTTGCTCA
ATGTGGCCTATCGGCACAGGCGGAAAACGGACGGTATCGGTCAGGGGGATTTTAAACTGTTGGCGGCACTGGGTGCCTGG
ACCGGCGGGGCCGCCCTGCCGATGCTGGTCAGCGGCGCCGCAGCGGCCGGGTTGTGCGCTACGGCAATAGCCCGTTTACT
GCGCAAGCCCGGCAGGCAGGATACGCTGCCCTTTGGCCTTTATCTGGCGCTTTCCGGCTGGTTGGTTCTGTTGGCGACCG
CCGATAGCGGCCCTTCATCTTTCAGCGGAGAAATCACAGCATTTCACCTTATTTCACCCTGA

Upstream 100 bases:

>100_bases
CCCCCTGCGCAATACCGGTAATCGATAAAACAATCGCTCTGCATGAAACCCTTTTTGCCGCTATTTTGAACGCTAATTTC
AGACCGGTAAGGAAGCTTTT

Downstream 100 bases:

>100_bases
TACCAGACTGGCCCGGCCGTTATATCGCTGTTATAGCCTACTGTTGGCGTAGCTGTTATGGCGCCCCTCCGCCTGCAGCA
CTATGCTTTGCCCAAGGCAA

Product: peptidase A24A prepilin type IV

Products: NA

Alternate protein names: Pectic enzymes secretion protein outO; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MFDNPQLDFWFRGLFIPAMLLPAALATRLIGILSPGLTQHPLPRERTLMLIAVSAGLAALPFNIPLLQRLLLLPAAAILL
ALALIDWQQRLLPDRLTQPLLWAGLLVNQQGYFVSLTEAVTGACIGYLSLWLLNVAYRHRRKTDGIGQGDFKLLAALGAW
TGGAALPMLVSGAAAAGLCATAIARLLRKPGRQDTLPFGLYLALSGWLVLLATADSGPSSFSGEITAFHLISP

Sequences:

>Translated_233_residues
MFDNPQLDFWFRGLFIPAMLLPAALATRLIGILSPGLTQHPLPRERTLMLIAVSAGLAALPFNIPLLQRLLLLPAAAILL
ALALIDWQQRLLPDRLTQPLLWAGLLVNQQGYFVSLTEAVTGACIGYLSLWLLNVAYRHRRKTDGIGQGDFKLLAALGAW
TGGAALPMLVSGAAAAGLCATAIARLLRKPGRQDTLPFGLYLALSGWLVLLATADSGPSSFSGEITAFHLISP
>Mature_233_residues
MFDNPQLDFWFRGLFIPAMLLPAALATRLIGILSPGLTQHPLPRERTLMLIAVSAGLAALPFNIPLLQRLLLLPAAAILL
ALALIDWQQRLLPDRLTQPLLWAGLLVNQQGYFVSLTEAVTGACIGYLSLWLLNVAYRHRRKTDGIGQGDFKLLAALGAW
TGGAALPMLVSGAAAAGLCATAIARLLRKPGRQDTLPFGLYLALSGWLVLLATADSGPSSFSGEITAFHLISP

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI1789732, Length=128, Percent_Identity=42.1875, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082194, Length=132, Percent_Identity=36.3636363636364, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 24955; Mature: 24955

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDNPQLDFWFRGLFIPAMLLPAALATRLIGILSPGLTQHPLPRERTLMLIAVSAGLAAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHC
PFNIPLLQRLLLLPAAAILLALALIDWQQRLLPDRLTQPLLWAGLLVNQQGYFVSLTEAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEHHHHH
TGACIGYLSLWLLNVAYRHRRKTDGIGQGDFKLLAALGAWTGGAALPMLVSGAAAAGLCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TAIARLLRKPGRQDTLPFGLYLALSGWLVLLATADSGPSSFSGEITAFHLISP
HHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MFDNPQLDFWFRGLFIPAMLLPAALATRLIGILSPGLTQHPLPRERTLMLIAVSAGLAAL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHC
PFNIPLLQRLLLLPAAAILLALALIDWQQRLLPDRLTQPLLWAGLLVNQQGYFVSLTEAV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEHHHHH
TGACIGYLSLWLLNVAYRHRRKTDGIGQGDFKLLAALGAWTGGAALPMLVSGAAAAGLCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TAIARLLRKPGRQDTLPFGLYLALSGWLVLLATADSGPSSFSGEITAFHLISP
HHHHHHHHCCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8326859; 8065262 [H]