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The map label for this gene is narK [H]

Identifier: 157371138

GI number: 157371138

Start: 3183925

End: 3185313

Strand: Reverse

Name: narK [H]

Synonym: Spro_2898

Alternate gene names: 157371138

Gene position: 3185313-3183925 (Counterclockwise)

Preceding gene: 157371141

Following gene: 157371137

Centisome position: 58.46

GC content: 56.66

Gene sequence:

>1389_bases
ATGTCGCAATCTGCAACGTCATTATCAAAACAAACAGGCGCGGTTATTCAGGACTGGCAGCCTGAGGATGCCGCATTCTG
GCAACAACGCGGCCAACGTATCGCCAACCGCAACCTGTGGATTTCCATTCCTTGCCTATTATTGGCTTTCTGCGTCTGGA
TGCTGTTTAGCGCGGTGGCGGTCAATTTGAATAAGGTTGGCTTTAATTTCACCACCGATCAGCTGTTTATGCTCACCGCG
TTACCTTCCGTTTCTGGCGCTATCCTGCGTGTGCCTTATTCCTTTGTGATCCCGATGTTCGGCGGCCGTCGCTGGACGGC
GATCAGCACCTGCCTGCTGCTGGTACCCTGCCTGTGGCTGGGGTTTGCGGTGCAGGACGCGACCACGCCATATAGCGTGT
TTGTGATTATCTCTTTACTGTGTGGTTTTGCCGGGGCCAACTTTGCCTCCAGCATGGCCAATATCAGCTTCTTCTTTCCA
AAGGCCAGACAGGGCGGCGCACTGGGCCTGAATGGCGGCCTGGGCAACCTGGGCGTTAGCGTGATGCAGTTGGTGGCTCC
ACTGGCCATCTCTGTTGCCATATTTGGCGCCTTTGGTGGTACCGGTCAGGTACAGGCTGATGGCAGCACACTGTGGCTGG
AGAACGCCGCCTGGATTTGGGTACCTTTCCTGCTGGTCGGTACGCTGGCGGCCTGGTTCGGTATGAACGATCTGGCGGCC
TCCAAAGCGTCGCTGCGTCAGCAACTGCCGGTACTCAAACGCGGTCACCTGTGGGTGTTGAGCCTGCTGTACCTCGCCAC
CTTTGGTTCCTTTATCGGTTTTTCCGCCGGTTTTGCCATGCTGTCCAAAACTCAGTTCCCGGACGTGGTGATTTTGCATT
ATGCCTTCTTCGGTCCGTTCCTCGGCGCTCTGGCACGGCCGGTGGGCGGGGCGCTGTCGGATCGCTTCGGCGGCATTCGC
GTCACGCTGATTAACTTTGTGCTGATGGCCGTGTTTGCTGCCTTGCTGTTCCTGACTTTACCGGGAGCAGGGCATACTGG
CTCGTTTATCGCATTTTATAGCGTCTTTATGCTGCTGTTCCTCACTGCGGGATTGGGCAGTGGTTCCACCTTCCAGATGA
TTGCCGTGATCTTCCGTAAAATTACCGTAGATCGGGTTAAGGCCAGCGGCGGCAGTGACGAAAGCGCTCAACGCGAAGCG
GTTACCGATTCCGCGGCAGCTCTGGGCTTCATCTCGGCGATCGGGGCCGCAGGCGGCTTCTTTATCCCAAAAGCCTTTGG
CACTTCCCTGGCGTTGACTGGCTCACCGGCAGGGGCGATGAAAATCTTCCTGGTGTTCTACGTGGTCTGTGTGCTGGTCA
CCTGGGTCGTCTACGGCCGTAAAAAATAG

Upstream 100 bases:

>100_bases
GGGCGGTTGGCTACCTGCATCCGGGTTGATTTACATCAACTTTGCCGTAGGGGCCGCCGCCTAGGGTGGGCGAAGTTATC
ATCCTGTGTCTGAGGTTTTT

Downstream 100 bases:

>100_bases
TTCACATATCCCCAGTTCCTCCCCGGCAGGGGAGGAGCTATTTCCCTTCTTTGCCATGTGAAGTTTTACTCCCTCAGTGA
AATCCCTGTCGCTCTAGGTA

Product: nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSQSATSLSKQTGAVIQDWQPEDAAFWQQRGQRIANRNLWISIPCLLLAFCVWMLFSAVAVNLNKVGFNFTTDQLFMLTA
LPSVSGAILRVPYSFVIPMFGGRRWTAISTCLLLVPCLWLGFAVQDATTPYSVFVIISLLCGFAGANFASSMANISFFFP
KARQGGALGLNGGLGNLGVSVMQLVAPLAISVAIFGAFGGTGQVQADGSTLWLENAAWIWVPFLLVGTLAAWFGMNDLAA
SKASLRQQLPVLKRGHLWVLSLLYLATFGSFIGFSAGFAMLSKTQFPDVVILHYAFFGPFLGALARPVGGALSDRFGGIR
VTLINFVLMAVFAALLFLTLPGAGHTGSFIAFYSVFMLLFLTAGLGSGSTFQMIAVIFRKITVDRVKASGGSDESAQREA
VTDSAAALGFISAIGAAGGFFIPKAFGTSLALTGSPAGAMKIFLVFYVVCVLVTWVVYGRKK

Sequences:

>Translated_462_residues
MSQSATSLSKQTGAVIQDWQPEDAAFWQQRGQRIANRNLWISIPCLLLAFCVWMLFSAVAVNLNKVGFNFTTDQLFMLTA
LPSVSGAILRVPYSFVIPMFGGRRWTAISTCLLLVPCLWLGFAVQDATTPYSVFVIISLLCGFAGANFASSMANISFFFP
KARQGGALGLNGGLGNLGVSVMQLVAPLAISVAIFGAFGGTGQVQADGSTLWLENAAWIWVPFLLVGTLAAWFGMNDLAA
SKASLRQQLPVLKRGHLWVLSLLYLATFGSFIGFSAGFAMLSKTQFPDVVILHYAFFGPFLGALARPVGGALSDRFGGIR
VTLINFVLMAVFAALLFLTLPGAGHTGSFIAFYSVFMLLFLTAGLGSGSTFQMIAVIFRKITVDRVKASGGSDESAQREA
VTDSAAALGFISAIGAAGGFFIPKAFGTSLALTGSPAGAMKIFLVFYVVCVLVTWVVYGRKK
>Mature_461_residues
SQSATSLSKQTGAVIQDWQPEDAAFWQQRGQRIANRNLWISIPCLLLAFCVWMLFSAVAVNLNKVGFNFTTDQLFMLTAL
PSVSGAILRVPYSFVIPMFGGRRWTAISTCLLLVPCLWLGFAVQDATTPYSVFVIISLLCGFAGANFASSMANISFFFPK
ARQGGALGLNGGLGNLGVSVMQLVAPLAISVAIFGAFGGTGQVQADGSTLWLENAAWIWVPFLLVGTLAAWFGMNDLAAS
KASLRQQLPVLKRGHLWVLSLLYLATFGSFIGFSAGFAMLSKTQFPDVVILHYAFFGPFLGALARPVGGALSDRFGGIRV
TLINFVLMAVFAALLFLTLPGAGHTGSFIAFYSVFMLLFLTAGLGSGSTFQMIAVIFRKITVDRVKASGGSDESAQREAV
TDSAAALGFISAIGAAGGFFIPKAFGTSLALTGSPAGAMKIFLVFYVVCVLVTWVVYGRKK

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=462, Percent_Identity=79.004329004329, Blast_Score=722, Evalue=0.0,
Organism=Escherichia coli, GI1787743, Length=454, Percent_Identity=73.7885462555066, Blast_Score=649, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49212; Mature: 49081

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSATSLSKQTGAVIQDWQPEDAAFWQQRGQRIANRNLWISIPCLLLAFCVWMLFSAVA
CCCCHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
VNLNKVGFNFTTDQLFMLTALPSVSGAILRVPYSFVIPMFGGRRWTAISTCLLLVPCLWL
HHHHHCCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GFAVQDATTPYSVFVIISLLCGFAGANFASSMANISFFFPKARQGGALGLNGGLGNLGVS
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCEEECCCCCCCCCEECCCCCHHHHHH
VMQLVAPLAISVAIFGAFGGTGQVQADGSTLWLENAAWIWVPFLLVGTLAAWFGMNDLAA
HHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
SKASLRQQLPVLKRGHLWVLSLLYLATFGSFIGFSAGFAMLSKTQFPDVVILHYAFFGPF
HHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LGALARPVGGALSDRFGGIRVTLINFVLMAVFAALLFLTLPGAGHTGSFIAFYSVFMLLF
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTAGLGSGSTFQMIAVIFRKITVDRVKASGGSDESAQREAVTDSAAALGFISAIGAAGGF
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FIPKAFGTSLALTGSPAGAMKIFLVFYVVCVLVTWVVYGRKK
CCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQSATSLSKQTGAVIQDWQPEDAAFWQQRGQRIANRNLWISIPCLLLAFCVWMLFSAVA
CCCHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
VNLNKVGFNFTTDQLFMLTALPSVSGAILRVPYSFVIPMFGGRRWTAISTCLLLVPCLWL
HHHHHCCCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GFAVQDATTPYSVFVIISLLCGFAGANFASSMANISFFFPKARQGGALGLNGGLGNLGVS
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCEEECCCCCCCCCEECCCCCHHHHHH
VMQLVAPLAISVAIFGAFGGTGQVQADGSTLWLENAAWIWVPFLLVGTLAAWFGMNDLAA
HHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
SKASLRQQLPVLKRGHLWVLSLLYLATFGSFIGFSAGFAMLSKTQFPDVVILHYAFFGPF
HHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LGALARPVGGALSDRFGGIRVTLINFVLMAVFAALLFLTLPGAGHTGSFIAFYSVFMLLF
HHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LTAGLGSGSTFQMIAVIFRKITVDRVKASGGSDESAQREAVTDSAAALGFISAIGAAGGF
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
FIPKAFGTSLALTGSPAGAMKIFLVFYVVCVLVTWVVYGRKK
CCHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]