Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 157370897

GI number: 157370897

Start: 2926204

End: 2928801

Strand: Reverse

Name: topA [H]

Synonym: Spro_2657

Alternate gene names: 157370897

Gene position: 2928801-2926204 (Counterclockwise)

Preceding gene: 157370899

Following gene: 157370896

Centisome position: 53.75

GC content: 54.73

Gene sequence:

>2598_bases
ATGGGTAAAGCTCTCGTAATAGTTGAGTCCCCGGCAAAAGCCAAAACTATTAATAAATATTTAGGTAATGACTACGTGGT
GAAGTCCAGCGTCGGTCATATCCGTGATTTGCCGACCAGTGGCTCAGCCAGCAAAAAGAGCGCTGACTCAACCGAAGACA
AAGCCAAGAAGAAAGTTAAAAAAGATCCAACGACGGCGCTGGTAAATCGTATGGGCGTCGATCCCTACCATGGCTGGAAA
GCGCATTACGAAATCCTGCCGGGTAAAGAAAAAGTTGTTGCCGAGTTAAAGTCGTTAGCGGAAAACGCCGACCACATTTA
TCTCGCAACCGACCTTGACCGCGAAGGGGAAGCCATTGCCTGGCACCTGCGGGAAGTTATCGGTGGTGACGACAAACGCT
TTAGCCGCGTGGTGTTTAACGAAATTACCAAGAACGCGATCCAAAACGCGTTCAAACAGCCCGGCGAACTGAATATCGAC
CGTGTCAACGCACAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGTTACATGGTCTCGCCACTGCTATGGAAAAAAAT
TGCCCGTGGCTTGTCCGCTGGGCGAGTGCAATCCGTTGCGGTTCGACTGGTGGTGGAGCGTGAGCGCGACATCAAAGCCT
TCGTGCCGGAAGAATACTGGGAGTTGCACGCCGATCTGTTGGCGAAGGGTGATACCGCGCTGCAGATGGAAGTGACCCAT
GCCCACGACAAACCCTTCAAACCGGTTAACCGTGAGCAGACTCACGCCGCACTCAACGTGCTGGAAAAAGCCCGTTATAC
GGTACTGGATCGCGAAGACAAGCCGACCAGCAGCAAGCCGGGCGCACCTTATATTACTTCTACGTTGCAGCAGGCGGCCA
GTACCCGTCTGAGCTTTGGCGTGAAGAAAACCATGATGATGGCTCAGCGCCTGTATGAAGCCGGTCACATCACCTATATG
CGTACCGACTCCACCAACCTGAGCCAGGATGCGCTCGAGATGGTGCGTGGCTACATCGGTGACAACTTCGGCGACAAGTA
CCTGCCGAAAGCGCCGAACCAGTACAGCAGCAAAGAAAACTCGCAGGAAGCACACGAAGCCATCCGTCCGTCGGATGTCA
ACGTGCTGGCCGAGCAGTTGAAAGATATGGAAGCGGATGCGCAAAAACTGTATCAGCTGATTTGGCGCCAGTTTGTGGCC
TGTCAGATGACACCGGCGCAGTACGACTCCACCACGCTGTCGGTGAAGGCCGCTGATTACCAGCTGCGCGCCAAAGGCCG
TACGCTGCGTTTCGATGGCTGGACCAAAGTGATGCCTGCTCTGCGTAAGGGTGATGAAGACCGCACCTTGCCGTATGTTG
AAGTAGGTAGCGATCTGGAACTGCAGAAACTGATCCCAAGCCAGCACTTTACCAAACCGCCGGCGCGTTATAGCGAAGCG
TCATTGGTAAAAGAGTTGGAAAAACGGGGTATCGGCCGTCCTTCAACCTACGCTTCGATCATTTCCACCATTCAGGATCG
TGGTTATGTGCGAGTCGAAAGCCGCCGTTTCTACGCTGAGAAAATGGGTGAGATCGTTACCGACCGCCTGGAAGAAAACT
TCCGCGAGCTGATGAACTATGACTTCACCGCACGCATGGAAAACGGTCTGGACCAGGTAGCCAACAACCAGGCCGAGTGG
AAAGCGGTGCTGGATGAGTTCTTTGCCGAGTTCAGCGAACAGCTGGAAACGGCGGAGAAAGAGCCGGAAGAAGGCGGTAT
GCGCCCGAATCAGATGGTGATGACCAGTATCGATTGCCCAACCTGCGGTCGCAAGATGGGTATCCGTACTGCCAGCACCG
GCGTTTTCCTGGGTTGCTCCGGCTATGCATTGCCGCCGAAGGAACGTTGTAAAACCACCATTAACCTGGTGCCGGAAACG
GAAGTGCTCAACATTCTCGAAGGGGATGACGCGGAAACCAACGCGTTGCGTGCCCGCCGTCGCTGCCAGAAATGCGGCAC
GGCGATGGACAGCTACCTGATCGATAACCAGCGCAAGCTGCACGTGTGCGGTAACAACCCGGCGTGTGACGGCTATGAGA
TTGAAGAGGGTGAGTTCCGCCTGAAAGGGTACGACGGCCCTGTGGTTGAGTGCGATAAGTGTGGTTCTGAAATGCACCTG
AAAATGGGGCGCTTCGGTAAGTACATGGGTTGCACCAACGAGAGCTGCAAAAATACCCGTAAGATCCTGCGTAATGGTGA
TGTTGCACCGCCGAAGGAAGATCCGGTTCCATTGCCGGAGCTGCCATGCGAAAAGTCGGACGCTTACTTTGTTCTGCGTG
ACGGCGCTGCCGGTGTGTTCCTGGCGGCCAATACCTTCCCTAAATCGCGCGAAACCCGTGCGCCGTTGGTGGAAGAGTTG
GCCCGCTTCAAGGACCGTCTGCCTGAAAAACTGCGTTATCTGGCGGATGCGCCGGTGGCCGATGCGGAAGGCAACAAAAC
GCTGGTGCGTTTCAGCCGCAAGACCAAGCAGCAGTATGTCTCGGCAGAGAAAGACGGCAAAGCCACTGGTTGGTCTGCTT
TCTACATCGATGGCAAATGGGTTGAAGGCAAAAAATAA

Upstream 100 bases:

>100_bases
TTGCCAGACTGCCGCAATATAAAAAAGAGATACGAATCGCTGCGGCAAGGCGAGATAAAAGACTTCTTTTTAGAAGAAAT
AAAATTAGGTAAAGGTAAAT

Downstream 100 bases:

>100_bases
ATTTGCAGCCTGAATGCAACATTAAAAACCAGCCCATGTGGCTGGTTTTTTTATTGGCCAAAGAGGGCTAACGGATCTAT
CCTTGGCTGTTTTTTTCCTC

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 865; Mature: 864

Protein sequence:

>865_residues
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTEDKAKKKVKKDPTTALVNRMGVDPYHGWK
AHYEILPGKEKVVAELKSLAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQNAFKQPGELNID
RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVERERDIKAFVPEEYWELHADLLAKGDTALQMEVTH
AHDKPFKPVNREQTHAALNVLEKARYTVLDREDKPTSSKPGAPYITSTLQQAASTRLSFGVKKTMMMAQRLYEAGHITYM
RTDSTNLSQDALEMVRGYIGDNFGDKYLPKAPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQKLYQLIWRQFVA
CQMTPAQYDSTTLSVKAADYQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPYVEVGSDLELQKLIPSQHFTKPPARYSEA
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLEENFRELMNYDFTARMENGLDQVANNQAEW
KAVLDEFFAEFSEQLETAEKEPEEGGMRPNQMVMTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPET
EVLNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEEGEFRLKGYDGPVVECDKCGSEMHL
KMGRFGKYMGCTNESCKNTRKILRNGDVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL
ARFKDRLPEKLRYLADAPVADAEGNKTLVRFSRKTKQQYVSAEKDGKATGWSAFYIDGKWVEGKK

Sequences:

>Translated_865_residues
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTEDKAKKKVKKDPTTALVNRMGVDPYHGWK
AHYEILPGKEKVVAELKSLAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQNAFKQPGELNID
RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVERERDIKAFVPEEYWELHADLLAKGDTALQMEVTH
AHDKPFKPVNREQTHAALNVLEKARYTVLDREDKPTSSKPGAPYITSTLQQAASTRLSFGVKKTMMMAQRLYEAGHITYM
RTDSTNLSQDALEMVRGYIGDNFGDKYLPKAPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQKLYQLIWRQFVA
CQMTPAQYDSTTLSVKAADYQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPYVEVGSDLELQKLIPSQHFTKPPARYSEA
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLEENFRELMNYDFTARMENGLDQVANNQAEW
KAVLDEFFAEFSEQLETAEKEPEEGGMRPNQMVMTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPET
EVLNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEEGEFRLKGYDGPVVECDKCGSEMHL
KMGRFGKYMGCTNESCKNTRKILRNGDVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEEL
ARFKDRLPEKLRYLADAPVADAEGNKTLVRFSRKTKQQYVSAEKDGKATGWSAFYIDGKWVEGKK
>Mature_864_residues
GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTEDKAKKKVKKDPTTALVNRMGVDPYHGWKA
HYEILPGKEKVVAELKSLAENADHIYLATDLDREGEAIAWHLREVIGGDDKRFSRVVFNEITKNAIQNAFKQPGELNIDR
VNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVERERDIKAFVPEEYWELHADLLAKGDTALQMEVTHA
HDKPFKPVNREQTHAALNVLEKARYTVLDREDKPTSSKPGAPYITSTLQQAASTRLSFGVKKTMMMAQRLYEAGHITYMR
TDSTNLSQDALEMVRGYIGDNFGDKYLPKAPNQYSSKENSQEAHEAIRPSDVNVLAEQLKDMEADAQKLYQLIWRQFVAC
QMTPAQYDSTTLSVKAADYQLRAKGRTLRFDGWTKVMPALRKGDEDRTLPYVEVGSDLELQKLIPSQHFTKPPARYSEAS
LVKELEKRGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLEENFRELMNYDFTARMENGLDQVANNQAEWK
AVLDEFFAEFSEQLETAEKEPEEGGMRPNQMVMTSIDCPTCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPETE
VLNILEGDDAETNALRARRRCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEEGEFRLKGYDGPVVECDKCGSEMHLK
MGRFGKYMGCTNESCKNTRKILRNGDVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVEELA
RFKDRLPEKLRYLADAPVADAEGNKTLVRFSRKTKQQYVSAEKDGKATGWSAFYIDGKWVEGKK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=567, Percent_Identity=26.4550264550265, Blast_Score=142, Evalue=2e-33,
Organism=Homo sapiens, GI4507635, Length=514, Percent_Identity=25.875486381323, Blast_Score=137, Evalue=5e-32,
Organism=Escherichia coli, GI1787529, Length=865, Percent_Identity=86.3583815028902, Blast_Score=1589, Evalue=0.0,
Organism=Escherichia coli, GI1788061, Length=548, Percent_Identity=22.992700729927, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=566, Percent_Identity=25.4416961130742, Blast_Score=136, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=509, Percent_Identity=24.5579567779961, Blast_Score=127, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6323263, Length=586, Percent_Identity=24.9146757679181, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24585251, Length=596, Percent_Identity=25, Blast_Score=137, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640096, Length=491, Percent_Identity=27.0875763747454, Blast_Score=115, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR013263
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08272 Topo_Zn_Ribbon; PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 97349; Mature: 97217

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTEDKAKKKVK
CCCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHH
KDPTTALVNRMGVDPYHGWKAHYEILPGKEKVVAELKSLAENADHIYLATDLDREGEAIA
CCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
WHLREVIGGDDKRFSRVVFNEITKNAIQNAFKQPGELNIDRVNAQQARRFMDRVVGYMVS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PLLWKKIARGLSAGRVQSVAVRLVVERERDIKAFVPEEYWELHADLLAKGDTALQMEVTH
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCEEEEEEEC
AHDKPFKPVNREQTHAALNVLEKARYTVLDREDKPTSSKPGAPYITSTLQQAASTRLSFG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VKKTMMMAQRLYEAGHITYMRTDSTNLSQDALEMVRGYIGDNFGDKYLPKAPNQYSSKEN
HHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SQEAHEAIRPSDVNVLAEQLKDMEADAQKLYQLIWRQFVACQMTPAQYDSTTLSVKAADY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCE
QLRAKGRTLRFDGWTKVMPALRKGDEDRTLPYVEVGSDLELQKLIPSQHFTKPPARYSEA
EEEECCCEEEECCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHCCCCCCCCCCCCCHHH
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLEENFRELMNY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DFTARMENGLDQVANNQAEWKAVLDEFFAEFSEQLETAEKEPEEGGMRPNQMVMTSIDCP
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCC
TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPETEVLNILEGDDAETNALRARR
CCCHHHCCEEECCCEEEECCCCCCCCHHHHCHHHCCCCCHHHHHHHCCCCCHHHHHHHHH
RCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEEGEFRLKGYDGPVVECDKCGSEMHL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCEEEHHHCCCHHHH
KMGRFGKYMGCTNESCKNTRKILRNGDVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
LAANTFPKSRETRAPLVEELARFKDRLPEKLRYLADAPVADAEGNKTLVRFSRKTKQQYV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
SAEKDGKATGWSAFYIDGKWVEGKK
CCCCCCCCCCEEEEEEECEECCCCC
>Mature Secondary Structure 
GKALVIVESPAKAKTINKYLGNDYVVKSSVGHIRDLPTSGSASKKSADSTEDKAKKKVK
CCEEEEEECCCHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHH
KDPTTALVNRMGVDPYHGWKAHYEILPGKEKVVAELKSLAENADHIYLATDLDREGEAIA
CCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHH
WHLREVIGGDDKRFSRVVFNEITKNAIQNAFKQPGELNIDRVNAQQARRFMDRVVGYMVS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
PLLWKKIARGLSAGRVQSVAVRLVVERERDIKAFVPEEYWELHADLLAKGDTALQMEVTH
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCCEEEEEEEC
AHDKPFKPVNREQTHAALNVLEKARYTVLDREDKPTSSKPGAPYITSTLQQAASTRLSFG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VKKTMMMAQRLYEAGHITYMRTDSTNLSQDALEMVRGYIGDNFGDKYLPKAPNQYSSKEN
HHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
SQEAHEAIRPSDVNVLAEQLKDMEADAQKLYQLIWRQFVACQMTPAQYDSTTLSVKAADY
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCE
QLRAKGRTLRFDGWTKVMPALRKGDEDRTLPYVEVGSDLELQKLIPSQHFTKPPARYSEA
EEEECCCEEEECCHHHHHHHHHCCCCCCCCCEEECCCCCHHHHHCCCCCCCCCCCCCHHH
SLVKELEKRGIGRPSTYASIISTIQDRGYVRVESRRFYAEKMGEIVTDRLEENFRELMNY
HHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DFTARMENGLDQVANNQAEWKAVLDEFFAEFSEQLETAEKEPEEGGMRPNQMVMTSIDCP
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEECCCC
TCGRKMGIRTASTGVFLGCSGYALPPKERCKTTINLVPETEVLNILEGDDAETNALRARR
CCCHHHCCEEECCCEEEECCCCCCCCHHHHCHHHCCCCCHHHHHHHCCCCCHHHHHHHHH
RCQKCGTAMDSYLIDNQRKLHVCGNNPACDGYEIEEGEFRLKGYDGPVVECDKCGSEMHL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCEEEECCCCCEEEHHHCCCHHHH
KMGRFGKYMGCTNESCKNTRKILRNGDVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVF
HHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEE
LAANTFPKSRETRAPLVEELARFKDRLPEKLRYLADAPVADAEGNKTLVRFSRKTKQQYV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
SAEKDGKATGWSAFYIDGKWVEGKK
CCCCCCCCCCEEEEEEECEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3029379; 9097039; 9278503; 3032952; 8114910; 7779808 [H]