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The map label for this gene is pcaB [H]

Identifier: 157370745

GI number: 157370745

Start: 2750184

End: 2751542

Strand: Reverse

Name: pcaB [H]

Synonym: Spro_2505

Alternate gene names: 157370745

Gene position: 2751542-2750184 (Counterclockwise)

Preceding gene: 157370746

Following gene: 157370744

Centisome position: 50.5

GC content: 60.12

Gene sequence:

>1359_bases
ATGGAGTTACTGACGCCGCTGCTGCGGTCTTCGGCGGTGACCCCTTGCTTTAGCGATACCGCTACGTTGCAGGGAATGCT
GGATTTCGAAGCAGCGCTGGCCAATGCACAGGCCGGGTTGGGCATTATTCCTGTCGAAGCTGCGCAGCACATTAGCGAGG
CTTGCCACAGCCATTTATTCGATGCGGCCGATCTGGCACAGGCGGCCGCGCAGGCAGGCAATCTGGCGATCCCGCTGGTT
AAGCAATTAACCCGCCAGGTGGCACAGCAAAATGCCGAGGCTGCACGTTTCGTTCATTGGGGGGCAACCAGCCAGGACGC
CATTGATACCGGGTTGATACTGCAACTGCGGCAAGCCATCGAACTGACGCACAGCGATCTGAACCGGCTGATTGCGGCGT
TGGTATCACAAATGACTCGCCATCAGGATTGCGTGCTGGTCGGCCGCACCTGGATGCAGCACGCTTTGCCCACCACGTTG
GGGTTAAAGCTGGCGGGCACGCTGGATGCCTTGTTGCGCTATCGACAGCGGCTGGATGAAATGCGGCCCCGCGTGTTGGC
ACTGCAATTGGGAGGCGCCGCCGGCACCCTGGCTTCTTTGGGTGAGCAGGGAAGTCTCCTGGTGACGGAGTTAGCCAGCA
GGCTGCAACTGACCGCCGCAAATACTCCCTGGCACAGTCAACGCGATCGCCTGTTGGAGATTGCCGGTTGGTATGCCGGG
TTATGTGGCTCGCTGGGCAAACTGGCGCGTGATATTTCGTTACTGACACAGACCGAAGTAGCGGAAGTTGCTGAACCGGT
GGCGGCTGGGCGCGGTGGCTCTTCGACCATGCCGCACAAGCGGAATCCGGTGGCCTGCGCGGTGATCCTCGCCGCGGCCA
ATCGGGTACCTGCGCTGGTTGGCGGGTTATATGCCGCCATGGTGCAGGAGCATGAACGCGGCCTGGGCGGTTGGCACGCC
GAATGGGAAAGCCTGCCGGAGTTGGTGATGTTAACGGCGGGGGCGTTGCACACGGCGGGTGATCTGCTGGACGGGCTGCA
GGTATTTCCAGCACAGATGGAACATAACCTGAAGTTAACCCACGGCCTGATCATGGCCGAGGCGGTCACTATGGCATTGG
GTGACGTCATGGGCCGCCAGCAAGCGCATCAGCATATCGAAAAACAGTGTCATCTGGCGCTGGCACAGCAGCTCACGCTG
CTAGAGGTGTTACTGGACGACCCGGTCGTCATGGCGCATTTATCTGCCCAGCGGCTGGCCGAGCTGTTGGATCCCCAGCA
GTACCTCGGCAGTGCAAAAGTCTTTACTCAGCGGGTGCTGGAACAGGCCGCAACTTCAGGAGCAAATCAACATGAATGA

Upstream 100 bases:

>100_bases
TCAATGTACCGATAATCGCCCAGCGTCGCGTTTAGCGGTCTTGACGCTGCTGACCGCGCCGCGCCATCATCGGATACTCG
TCTTTTTGCCTGGAGGAACT

Downstream 100 bases:

>100_bases
TCAACAACGTTATCAGCAGGGCATTGAGGTGCGCAGAGCGGTACTGGGGGATGCACATGTGGATCGTACCCTCAGCCAGC
TCACCCCGTTAAATGAAGAG

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MELLTPLLRSSAVTPCFSDTATLQGMLDFEAALANAQAGLGIIPVEAAQHISEACHSHLFDAADLAQAAAQAGNLAIPLV
KQLTRQVAQQNAEAARFVHWGATSQDAIDTGLILQLRQAIELTHSDLNRLIAALVSQMTRHQDCVLVGRTWMQHALPTTL
GLKLAGTLDALLRYRQRLDEMRPRVLALQLGGAAGTLASLGEQGSLLVTELASRLQLTAANTPWHSQRDRLLEIAGWYAG
LCGSLGKLARDISLLTQTEVAEVAEPVAAGRGGSSTMPHKRNPVACAVILAAANRVPALVGGLYAAMVQEHERGLGGWHA
EWESLPELVMLTAGALHTAGDLLDGLQVFPAQMEHNLKLTHGLIMAEAVTMALGDVMGRQQAHQHIEKQCHLALAQQLTL
LEVLLDDPVVMAHLSAQRLAELLDPQQYLGSAKVFTQRVLEQAATSGANQHE

Sequences:

>Translated_452_residues
MELLTPLLRSSAVTPCFSDTATLQGMLDFEAALANAQAGLGIIPVEAAQHISEACHSHLFDAADLAQAAAQAGNLAIPLV
KQLTRQVAQQNAEAARFVHWGATSQDAIDTGLILQLRQAIELTHSDLNRLIAALVSQMTRHQDCVLVGRTWMQHALPTTL
GLKLAGTLDALLRYRQRLDEMRPRVLALQLGGAAGTLASLGEQGSLLVTELASRLQLTAANTPWHSQRDRLLEIAGWYAG
LCGSLGKLARDISLLTQTEVAEVAEPVAAGRGGSSTMPHKRNPVACAVILAAANRVPALVGGLYAAMVQEHERGLGGWHA
EWESLPELVMLTAGALHTAGDLLDGLQVFPAQMEHNLKLTHGLIMAEAVTMALGDVMGRQQAHQHIEKQCHLALAQQLTL
LEVLLDDPVVMAHLSAQRLAELLDPQQYLGSAKVFTQRVLEQAATSGANQHE
>Mature_452_residues
MELLTPLLRSSAVTPCFSDTATLQGMLDFEAALANAQAGLGIIPVEAAQHISEACHSHLFDAADLAQAAAQAGNLAIPLV
KQLTRQVAQQNAEAARFVHWGATSQDAIDTGLILQLRQAIELTHSDLNRLIAALVSQMTRHQDCVLVGRTWMQHALPTTL
GLKLAGTLDALLRYRQRLDEMRPRVLALQLGGAAGTLASLGEQGSLLVTELASRLQLTAANTPWHSQRDRLLEIAGWYAG
LCGSLGKLARDISLLTQTEVAEVAEPVAAGRGGSSTMPHKRNPVACAVILAAANRVPALVGGLYAAMVQEHERGLGGWHA
EWESLPELVMLTAGALHTAGDLLDGLQVFPAQMEHNLKLTHGLIMAEAVTMALGDVMGRQQAHQHIEKQCHLALAQQLTL
LEVLLDDPVVMAHLSAQRLAELLDPQQYLGSAKVFTQRVLEQAATSGANQHE

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=368, Percent_Identity=28.804347826087, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI183227688, Length=324, Percent_Identity=27.4691358024691, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI31541964, Length=207, Percent_Identity=31.8840579710145, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI68303542, Length=207, Percent_Identity=31.8840579710145, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI68303547, Length=200, Percent_Identity=32, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17508577, Length=313, Percent_Identity=26.1980830670927, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=434, Percent_Identity=24.8847926267281, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24640179, Length=353, Percent_Identity=26.3456090651558, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24640177, Length=353, Percent_Identity=26.3456090651558, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI78710009, Length=347, Percent_Identity=26.2247838616715, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24647570, Length=372, Percent_Identity=26.8817204301075, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 48395; Mature: 48395

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELLTPLLRSSAVTPCFSDTATLQGMLDFEAALANAQAGLGIIPVEAAQHISEACHSHLF
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCC
DAADLAQAAAQAGNLAIPLVKQLTRQVAQQNAEAARFVHWGATSQDAIDTGLILQLRQAI
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHHHHHHHHHH
ELTHSDLNRLIAALVSQMTRHQDCVLVGRTWMQHALPTTLGLKLAGTLDALLRYRQRLDE
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MRPRVLALQLGGAAGTLASLGEQGSLLVTELASRLQLTAANTPWHSQRDRLLEIAGWYAG
HCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
LCGSLGKLARDISLLTQTEVAEVAEPVAAGRGGSSTMPHKRNPVACAVILAAANRVPALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGLYAAMVQEHERGLGGWHAEWESLPELVMLTAGALHTAGDLLDGLQVFPAQMEHNLKLT
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HGLIMAEAVTMALGDVMGRQQAHQHIEKQCHLALAQQLTLLEVLLDDPVVMAHLSAQRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ELLDPQQYLGSAKVFTQRVLEQAATSGANQHE
HHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MELLTPLLRSSAVTPCFSDTATLQGMLDFEAALANAQAGLGIIPVEAAQHISEACHSHLF
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCC
DAADLAQAAAQAGNLAIPLVKQLTRQVAQQNAEAARFVHWGATSQDAIDTGLILQLRQAI
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHHHHHHHHHH
ELTHSDLNRLIAALVSQMTRHQDCVLVGRTWMQHALPTTLGLKLAGTLDALLRYRQRLDE
HHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
MRPRVLALQLGGAAGTLASLGEQGSLLVTELASRLQLTAANTPWHSQRDRLLEIAGWYAG
HCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHH
LCGSLGKLARDISLLTQTEVAEVAEPVAAGRGGSSTMPHKRNPVACAVILAAANRVPALV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GGLYAAMVQEHERGLGGWHAEWESLPELVMLTAGALHTAGDLLDGLQVFPAQMEHNLKLT
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
HGLIMAEAVTMALGDVMGRQQAHQHIEKQCHLALAQQLTLLEVLLDDPVVMAHLSAQRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ELLDPQQYLGSAKVFTQRVLEQAATSGANQHE
HHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]