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The map label for this gene is dfrA [H]

Identifier: 157370612

GI number: 157370612

Start: 2587670

End: 2588689

Strand: Reverse

Name: dfrA [H]

Synonym: Spro_2372

Alternate gene names: 157370612

Gene position: 2588689-2587670 (Counterclockwise)

Preceding gene: 157370614

Following gene: 157370610

Centisome position: 47.51

GC content: 55.59

Gene sequence:

>1020_bases
ATGATGAAAATAGAAACGGCATTTGTCACCGGCGCGACCGGTTTGCTCGGTAATAATCTGGTGCGTGAGTTGATCTCTCG
GGGTGCCAGGGTCAAAGCTCTGGTTCGTTCCACGGAAAAAGGTCGCCAGCAGTTCGGGGAGATTGAAGGCGTTGAATTAA
TCGCAGGCGATATGACTGACGTCGCCGCCTTCGCTGAACACCTGCAAGGCTGCGACATCTTGTTCCATACCGCGGCCTAC
TTCCGTGATAATTACAAGGGCGGTAGCCATTGGCCGAAGCTGAAAGCCATCAACGTCGAGGGTACCAGACATCTGCTGGA
ACAGGCTTACCATGCAGGGTTACGTCGTTTTATCCATACCTCATCTATCGCCGTGCTCAACGGCGAACCGGGCCAGTCGA
TCGACGAAACCTGTTTACGGCGGCCCGAAGATGCCGATGATTACTACCGCAGTAAAATACTGGCGGATGACGTGGTTTTG
GAATTCCTGCGGCAGCATCCAGAAATGAACGGCAGCCTGATCTTGCCGGGCTGGATGTGGGGGCCGGGCGATCTTGGCCC
CACCTCTTCCGGTCAGTTGGCCAATGACGTGATGCAGGGGAAATTACCGGGACTGGTGACGGGCAGTTTTTCTGTGGTTG
ATGCACGAGATGTGGCACTGGCCATGATACTGGCGGCAGAGCGAGGCCAAGGAGGTGAACGTTATTTGGCCGCAGGACAA
CACATGACCATGCACCAGTTGGTCCCCATGCTGGGGGATATTGCCGGTGTCAAAACCCCCACCCGCACGTTGCCACTGCC
ATTTCTCTACCTGTTGGCGACGCTACAGGAGGCCTACGCCCGCCTCAGTGGCAAACCGGTGTTGCTCAGCCTGGCCACGG
TGCGTTTGATGGTAAAAGAGGCCAACCGTTCTCATTTCAATCACGCCAAAAGTGAACGTGACCTGGGGCTGACATTCCGA
CCACTGGAACAAACGCTTCAGGATACCGTTGCCTGGCTGCGTAATAACAGGCCAACCTAA

Upstream 100 bases:

>100_bases
CACTACTCTATAAGCATTCAAGATATCACTTGATATTTTAAACCTCTACCACTAGATTTGGATATCACTTGAACTTTGAA
ATTCACTTAAGATTTTAATC

Downstream 100 bases:

>100_bases
TCCCCCGGGCAAGGGGAAACAGGGTTCCCCAGCCGTTGTCAGTTACGCCGTCTGCGACTCCACATAGCCCAGGGCCGAGC
GCAATGTGGTTTCGTTAAGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MMKIETAFVTGATGLLGNNLVRELISRGARVKALVRSTEKGRQQFGEIEGVELIAGDMTDVAAFAEHLQGCDILFHTAAY
FRDNYKGGSHWPKLKAINVEGTRHLLEQAYHAGLRRFIHTSSIAVLNGEPGQSIDETCLRRPEDADDYYRSKILADDVVL
EFLRQHPEMNGSLILPGWMWGPGDLGPTSSGQLANDVMQGKLPGLVTGSFSVVDARDVALAMILAAERGQGGERYLAAGQ
HMTMHQLVPMLGDIAGVKTPTRTLPLPFLYLLATLQEAYARLSGKPVLLSLATVRLMVKEANRSHFNHAKSERDLGLTFR
PLEQTLQDTVAWLRNNRPT

Sequences:

>Translated_339_residues
MMKIETAFVTGATGLLGNNLVRELISRGARVKALVRSTEKGRQQFGEIEGVELIAGDMTDVAAFAEHLQGCDILFHTAAY
FRDNYKGGSHWPKLKAINVEGTRHLLEQAYHAGLRRFIHTSSIAVLNGEPGQSIDETCLRRPEDADDYYRSKILADDVVL
EFLRQHPEMNGSLILPGWMWGPGDLGPTSSGQLANDVMQGKLPGLVTGSFSVVDARDVALAMILAAERGQGGERYLAAGQ
HMTMHQLVPMLGDIAGVKTPTRTLPLPFLYLLATLQEAYARLSGKPVLLSLATVRLMVKEANRSHFNHAKSERDLGLTFR
PLEQTLQDTVAWLRNNRPT
>Mature_339_residues
MMKIETAFVTGATGLLGNNLVRELISRGARVKALVRSTEKGRQQFGEIEGVELIAGDMTDVAAFAEHLQGCDILFHTAAY
FRDNYKGGSHWPKLKAINVEGTRHLLEQAYHAGLRRFIHTSSIAVLNGEPGQSIDETCLRRPEDADDYYRSKILADDVVL
EFLRQHPEMNGSLILPGWMWGPGDLGPTSSGQLANDVMQGKLPGLVTGSFSVVDARDVALAMILAAERGQGGERYLAAGQ
HMTMHQLVPMLGDIAGVKTPTRTLPLPFLYLLATLQEAYARLSGKPVLLSLATVRLMVKEANRSHFNHAKSERDLGLTFR
PLEQTLQDTVAWLRNNRPT

Specific function: Unknown

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI260763931, Length=366, Percent_Identity=26.5027322404372, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI4504509, Length=366, Percent_Identity=26.5027322404372, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI116268111, Length=369, Percent_Identity=25.7452574525745, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504507, Length=361, Percent_Identity=26.0387811634349, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI239745448, Length=344, Percent_Identity=25.2906976744186, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310132178, Length=344, Percent_Identity=25.2906976744186, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310113012, Length=344, Percent_Identity=25.2906976744186, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081792, Length=347, Percent_Identity=27.0893371757925, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71987463, Length=344, Percent_Identity=27.0348837209302, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6321437, Length=353, Percent_Identity=24.0793201133144, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 37325; Mature: 37325

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKIETAFVTGATGLLGNNLVRELISRGARVKALVRSTEKGRQQFGEIEGVELIAGDMTD
CCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHH
VAAFAEHLQGCDILFHTAAYFRDNYKGGSHWPKLKAINVEGTRHLLEQAYHAGLRRFIHT
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
SSIAVLNGEPGQSIDETCLRRPEDADDYYRSKILADDVVLEFLRQHPEMNGSLILPGWMW
CCEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
GPGDLGPTSSGQLANDVMQGKLPGLVTGSFSVVDARDVALAMILAAERGQGGERYLAAGQ
CCCCCCCCCCCHHHHHHHHCCCCCEEECCEEEECHHHHHHHHHHHHHCCCCCCHHHHCCC
HMTMHQLVPMLGDIAGVKTPTRTLPLPFLYLLATLQEAYARLSGKPVLLSLATVRLMVKE
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ANRSHFNHAKSERDLGLTFRPLEQTLQDTVAWLRNNRPT
HHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMKIETAFVTGATGLLGNNLVRELISRGARVKALVRSTEKGRQQFGEIEGVELIAGDMTD
CCEEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHH
VAAFAEHLQGCDILFHTAAYFRDNYKGGSHWPKLKAINVEGTRHLLEQAYHAGLRRFIHT
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
SSIAVLNGEPGQSIDETCLRRPEDADDYYRSKILADDVVLEFLRQHPEMNGSLILPGWMW
CCEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCC
GPGDLGPTSSGQLANDVMQGKLPGLVTGSFSVVDARDVALAMILAAERGQGGERYLAAGQ
CCCCCCCCCCCHHHHHHHHCCCCCEEECCEEEECHHHHHHHHHHHHHCCCCCCHHHHCCC
HMTMHQLVPMLGDIAGVKTPTRTLPLPFLYLLATLQEAYARLSGKPVLLSLATVRLMVKE
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ANRSHFNHAKSERDLGLTFRPLEQTLQDTVAWLRNNRPT
HHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]