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The map label for this gene is yfhM [H]

Identifier: 157370572

GI number: 157370572

Start: 2544409

End: 2545416

Strand: Reverse

Name: yfhM [H]

Synonym: Spro_2332

Alternate gene names: 157370572

Gene position: 2545416-2544409 (Counterclockwise)

Preceding gene: 157370573

Following gene: 157370571

Centisome position: 46.71

GC content: 55.56

Gene sequence:

>1008_bases
ATGCTGAGATTTCCCCGTCGTTGGCTGTTATCCGTCGCCCTCACTGCTTTTACCGCCACAGCAGCGACCGAATCGCCAAA
ATACGGCCAAGAGCTGCAGGGTTTTCATTACCCTTACCCCTTGCAGCAGTTTAGCTTCACCTCTCAGGGGCAGCAGTTAA
GCATGGGGTATATGGACGTGCCGCCAGAAGGTGAAGCCAACGGTCACACTGCGGTGCTGATGCACGGTAAGAACTTCTGC
GCCGCCACCTGGCAAGACACTATCAAAGCGCTGAGCAAAGCCGGTTACCGGGTGATCGCGCCGGACCAAATCGGCTTTTG
CAGTTCGACCAAACCGCAACATTACCAATACAGTTTCCAGCAGTTGGCGCAGAACACCCACGCGTTGCTCAATAACCTGA
AAATCAGTAAAGCGACTATCGTCGGCCACTCTACCGGCGGCATGCTGGCGACGCGTTACAGCCTGATGTATCCGCAGGCG
GTTGAGCGACTGGTAATGGTGAACCCGATTGGGTTGGAAGACTGGAAGGCCAAGGGTGTTCCCTGGCGCAGCGTCGATCA
ATGGTTCGAGCGTGAGCTGAAAACTTCCGCTGACGGAATTCGGCAATATGAGTTGAATACCTACTACGTCGGCCGCTGGA
AACCAGAGTATGACCGCTGGGTCGATATGCTCGCCGGGCTGAACAACGGGCCGGGGCATCGCCTGGTGGCCTGGAACTCG
GCGCTGATTTACGACATGATTTTTACCCAGCCGGTGTACTACGAATTTAAGGATCTGCAAACGCCAACCACGCTGATGAT
TGGTACCGCCGACACCACCGCCATCGGCAGTGACATTGCCCCGCCGGCGGTCAAGGCTACACTTGGTCATTACAATGTGT
TGGGCAAACAGGTGGCAAAACTGATCCCGCACGCCCGTCTGATCGAGTTTCCGGGACTGGGCCATGCGCCGCAAATGGAA
GAACCCGCACAGTTTAATCAGGCGTTGATTAAAGCCTTGCAACCATAA

Upstream 100 bases:

>100_bases
CTCGGCATGTCACCCAAGGCACAAGCGGCGGCGTCGCCCTGAAAACTCAACCATTAGGGCTATACTCAGACTCTTGTCGA
TTTTTCATAAGGACATCAGG

Downstream 100 bases:

>100_bases
CGATCTCGCTACAGGACAGGAAACCACCAATGCTTGATATAAAAATTCGCCGGCTCCAGGCAGAAAACGCAGAGGATTTT
CGGGCTATTCGGCTTGCCGC

Product: alpha/beta hydrolase fold domain-containing protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MLRFPRRWLLSVALTAFTATAATESPKYGQELQGFHYPYPLQQFSFTSQGQQLSMGYMDVPPEGEANGHTAVLMHGKNFC
AATWQDTIKALSKAGYRVIAPDQIGFCSSTKPQHYQYSFQQLAQNTHALLNNLKISKATIVGHSTGGMLATRYSLMYPQA
VERLVMVNPIGLEDWKAKGVPWRSVDQWFERELKTSADGIRQYELNTYYVGRWKPEYDRWVDMLAGLNNGPGHRLVAWNS
ALIYDMIFTQPVYYEFKDLQTPTTLMIGTADTTAIGSDIAPPAVKATLGHYNVLGKQVAKLIPHARLIEFPGLGHAPQME
EPAQFNQALIKALQP

Sequences:

>Translated_335_residues
MLRFPRRWLLSVALTAFTATAATESPKYGQELQGFHYPYPLQQFSFTSQGQQLSMGYMDVPPEGEANGHTAVLMHGKNFC
AATWQDTIKALSKAGYRVIAPDQIGFCSSTKPQHYQYSFQQLAQNTHALLNNLKISKATIVGHSTGGMLATRYSLMYPQA
VERLVMVNPIGLEDWKAKGVPWRSVDQWFERELKTSADGIRQYELNTYYVGRWKPEYDRWVDMLAGLNNGPGHRLVAWNS
ALIYDMIFTQPVYYEFKDLQTPTTLMIGTADTTAIGSDIAPPAVKATLGHYNVLGKQVAKLIPHARLIEFPGLGHAPQME
EPAQFNQALIKALQP
>Mature_335_residues
MLRFPRRWLLSVALTAFTATAATESPKYGQELQGFHYPYPLQQFSFTSQGQQLSMGYMDVPPEGEANGHTAVLMHGKNFC
AATWQDTIKALSKAGYRVIAPDQIGFCSSTKPQHYQYSFQQLAQNTHALLNNLKISKATIVGHSTGGMLATRYSLMYPQA
VERLVMVNPIGLEDWKAKGVPWRSVDQWFERELKTSADGIRQYELNTYYVGRWKPEYDRWVDMLAGLNNGPGHRLVAWNS
ALIYDMIFTQPVYYEFKDLQTPTTLMIGTADTTAIGSDIAPPAVKATLGHYNVLGKQVAKLIPHARLIEFPGLGHAPQME
EPAQFNQALIKALQP

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI27597073, Length=354, Percent_Identity=25.9887005649718, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 37561; Mature: 37561

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFPRRWLLSVALTAFTATAATESPKYGQELQGFHYPYPLQQFSFTSQGQQLSMGYMDV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHCCCCCCCCEEECCEECC
PPEGEANGHTAVLMHGKNFCAATWQDTIKALSKAGYRVIAPDQIGFCSSTKPQHYQYSFQ
CCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHH
QLAQNTHALLNNLKISKATIVGHSTGGMLATRYSLMYPQAVERLVMVNPIGLEDWKAKGV
HHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCC
PWRSVDQWFERELKTSADGIRQYELNTYYVGRWKPEYDRWVDMLAGLNNGPGHRLVAWNS
CHHHHHHHHHHHHHHHHCCCEEEEECEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECC
ALIYDMIFTQPVYYEFKDLQTPTTLMIGTADTTAIGSDIAPPAVKATLGHYNVLGKQVAK
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LIPHARLIEFPGLGHAPQMEEPAQFNQALIKALQP
HCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRFPRRWLLSVALTAFTATAATESPKYGQELQGFHYPYPLQQFSFTSQGQQLSMGYMDV
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCHHHCCCCCCCCEEECCEECC
PPEGEANGHTAVLMHGKNFCAATWQDTIKALSKAGYRVIAPDQIGFCSSTKPQHYQYSFQ
CCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHH
QLAQNTHALLNNLKISKATIVGHSTGGMLATRYSLMYPQAVERLVMVNPIGLEDWKAKGV
HHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCC
PWRSVDQWFERELKTSADGIRQYELNTYYVGRWKPEYDRWVDMLAGLNNGPGHRLVAWNS
CHHHHHHHHHHHHHHHHCCCEEEEECEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECC
ALIYDMIFTQPVYYEFKDLQTPTTLMIGTADTTAIGSDIAPPAVKATLGHYNVLGKQVAK
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LIPHARLIEFPGLGHAPQMEEPAQFNQALIKALQP
HCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]