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The map label for this gene is guaD [H]

Identifier: 157370539

GI number: 157370539

Start: 2502754

End: 2504085

Strand: Direct

Name: guaD [H]

Synonym: Spro_2299

Alternate gene names: 157370539

Gene position: 2502754-2504085 (Clockwise)

Preceding gene: 157370538

Following gene: 157370540

Centisome position: 45.93

GC content: 57.43

Gene sequence:

>1332_bases
ATGAGCCTGTCATTCACCACGGCGATCCGTGGCAATTTTTTCGATATTGCTGCCTGTGTCGATCAACCGCAGCAGTTGGA
TCAGCAACTGCGACATATTCCTGACGGATTAATGCTGCTCGATGAAGGGCACATTGTCTGGTTTGGCCCCTGGCAGCAGG
GGGAACCGCTGTTGTCACCGGGGTTTGAGGTCACTCATTATCAGGACAAACTGATAGTGCCGGGCTTTATCGATACCCAT
ATTCACTACCCGCAAACCGAAATGATCGGTGCCTATGGCGATCGGCTGCTGGCATGGCTGAAGCATTACACCTTCCCGAC
GGAAAGCCAATTCTGCTGCGAGCAGCACTCCGGCAAGATGTCGGCGTTCTTTTTACAGCAACTGTTAAGCAACGGCACCA
CCACGGCGCTGGTGTTCGGCACCGTACACCCGCAGTCGGTTGAAGCGCTGTTCAGTCAGGCGGCGGCGCTGAATATGCGG
CTGATCGCCGGCAAGGTGATGATGGACCGCCATGCGCCTGAGGAACTGCTGGAAACCCCGGAGCAGAGCTACCGCCAGAC
CCGCGAACTGATTGAGCGCTGGCACGGCAAGCAACGGCTGAGCTATGCCATCACGCCGCGTTTTGCGCCCACCAGCACCC
CGGCGCTGTTGGCTCAGGTACAAAGGTTGCGTGAAGAGTTTCCGGATGCCTATCTGCACACTCACCTGAGCGAGAACCTG
GAGGAAGTCGCCTGGGTGAAAGAACTGTTCCCGGACAGCCGCAGTTACCTGGATGTCTATCACCAGTATGGGTTGACCGG
AGAGAGAAGCGTGTTTGCCCATTGCCTGCATCTGGAAGAGCAAGAGTGGGATTGCCTGTGTCACACCGGTTCGGCCATCG
CCTTTTGTCCGACCTCCAATTTGTTCCTCGGCAGCGGCTTGTTCAACCTGCAAAAGGCCTGGCAGAAACAGGTCAGGCTG
GGTATTGGCACCGACGTCGGTGCCGGTACCACCTTCAATATGCTGCAAACGCTGGGCGAAGCTTACAAGGTCGCTCAACT
GCAGCGCTACCAGCTCTCGGCCTGCGAAGCCTTCTACCACGCCACATTGGGTGGGGCGCGGGCGCTGTCGCTGGATGACA
AAATCGGTAATTTCTCGGTCGGCAAAGAGGCGGATTTTGTGGTGCTGGATCCGGCGGTATCGCCGCTGCAAAAACTGCGG
CATGAAAACAGCCGTGAGCTGGCGGAGCAACTGTTTGTGTTGATGACGCTGGGCGATGACCGCAACGTTTACCGAACCTA
CGTCGACGGTAAAGTGGTGTACCGACCGGCTACGCAGCAGGAGGCGGCATGA

Upstream 100 bases:

>100_bases
TTAAATCAACACCTGCAAACGATAAAAGACCAGCATGGCTCCCCGGGCTGAATAAACTCAGCCGGGGGCTGTGCGCTGGC
CGGTAGAGAAGGAAAGCAAA

Downstream 100 bases:

>100_bases
TCCAGTTTCTGCTTAATCAAACGCTGAAAAATGAAAGCGCGATCGACCCCAATACCACGGTGCTTAATTATCTGCGCCGC
GATCTCGGCCGCTGTGGCAC

Product: guanine deaminase

Products: NA

Alternate protein names: Guanase; Guanine aminase; Guanine aminohydrolase; GAH [H]

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSLSFTTAIRGNFFDIAACVDQPQQLDQQLRHIPDGLMLLDEGHIVWFGPWQQGEPLLSPGFEVTHYQDKLIVPGFIDTH
IHYPQTEMIGAYGDRLLAWLKHYTFPTESQFCCEQHSGKMSAFFLQQLLSNGTTTALVFGTVHPQSVEALFSQAAALNMR
LIAGKVMMDRHAPEELLETPEQSYRQTRELIERWHGKQRLSYAITPRFAPTSTPALLAQVQRLREEFPDAYLHTHLSENL
EEVAWVKELFPDSRSYLDVYHQYGLTGERSVFAHCLHLEEQEWDCLCHTGSAIAFCPTSNLFLGSGLFNLQKAWQKQVRL
GIGTDVGAGTTFNMLQTLGEAYKVAQLQRYQLSACEAFYHATLGGARALSLDDKIGNFSVGKEADFVVLDPAVSPLQKLR
HENSRELAEQLFVLMTLGDDRNVYRTYVDGKVVYRPATQQEAA

Sequences:

>Translated_443_residues
MSLSFTTAIRGNFFDIAACVDQPQQLDQQLRHIPDGLMLLDEGHIVWFGPWQQGEPLLSPGFEVTHYQDKLIVPGFIDTH
IHYPQTEMIGAYGDRLLAWLKHYTFPTESQFCCEQHSGKMSAFFLQQLLSNGTTTALVFGTVHPQSVEALFSQAAALNMR
LIAGKVMMDRHAPEELLETPEQSYRQTRELIERWHGKQRLSYAITPRFAPTSTPALLAQVQRLREEFPDAYLHTHLSENL
EEVAWVKELFPDSRSYLDVYHQYGLTGERSVFAHCLHLEEQEWDCLCHTGSAIAFCPTSNLFLGSGLFNLQKAWQKQVRL
GIGTDVGAGTTFNMLQTLGEAYKVAQLQRYQLSACEAFYHATLGGARALSLDDKIGNFSVGKEADFVVLDPAVSPLQKLR
HENSRELAEQLFVLMTLGDDRNVYRTYVDGKVVYRPATQQEAA
>Mature_442_residues
SLSFTTAIRGNFFDIAACVDQPQQLDQQLRHIPDGLMLLDEGHIVWFGPWQQGEPLLSPGFEVTHYQDKLIVPGFIDTHI
HYPQTEMIGAYGDRLLAWLKHYTFPTESQFCCEQHSGKMSAFFLQQLLSNGTTTALVFGTVHPQSVEALFSQAAALNMRL
IAGKVMMDRHAPEELLETPEQSYRQTRELIERWHGKQRLSYAITPRFAPTSTPALLAQVQRLREEFPDAYLHTHLSENLE
EVAWVKELFPDSRSYLDVYHQYGLTGERSVFAHCLHLEEQEWDCLCHTGSAIAFCPTSNLFLGSGLFNLQKAWQKQVRLG
IGTDVGAGTTFNMLQTLGEAYKVAQLQRYQLSACEAFYHATLGGARALSLDDKIGNFSVGKEADFVVLDPAVSPLQKLRH
ENSRELAEQLFVLMTLGDDRNVYRTYVDGKVVYRPATQQEAA

Specific function: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia [H]

COG id: COG0402

COG function: function code FR; Cytosine deaminase and related metal-dependent hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATZ/TRZ family [H]

Homologues:

Organism=Homo sapiens, GI4758426, Length=413, Percent_Identity=33.8983050847458, Blast_Score=223, Evalue=4e-58,
Organism=Escherichia coli, GI1789249, Length=427, Percent_Identity=64.8711943793911, Blast_Score=611, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6319963, Length=327, Percent_Identity=35.1681957186544, Blast_Score=216, Evalue=4e-57,
Organism=Drosophila melanogaster, GI24643849, Length=427, Percent_Identity=34.6604215456674, Blast_Score=223, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR014311
- InterPro:   IPR011059 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: =3.5.4.3 [H]

Molecular weight: Translated: 50079; Mature: 49948

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSFTTAIRGNFFDIAACVDQPQQLDQQLRHIPDGLMLLDEGHIVWFGPWQQGEPLLSP
CCCEEEEHHCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCC
GFEVTHYQDKLIVPGFIDTHIHYPQTEMIGAYGDRLLAWLKHYTFPTESQFCCEQHSGKM
CCEEEEECCCEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCH
SAFFLQQLLSNGTTTALVFGTVHPQSVEALFSQAAALNMRLIAGKVMMDRHAPEELLETP
HHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
EQSYRQTRELIERWHGKQRLSYAITPRFAPTSTPALLAQVQRLREEFPDAYLHTHLSENL
HHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
EEVAWVKELFPDSRSYLDVYHQYGLTGERSVFAHCLHLEEQEWDCLCHTGSAIAFCPTSN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCEEEECCCCC
LFLGSGLFNLQKAWQKQVRLGIGTDVGAGTTFNMLQTLGEAYKVAQLQRYQLSACEAFYH
CEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLGGARALSLDDKIGNFSVGKEADFVVLDPAVSPLQKLRHENSRELAEQLFVLMTLGDD
HHCCCCEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHEEEEEECCC
RNVYRTYVDGKVVYRPATQQEAA
CCEEEEEECCEEEECCCCCCCCC
>Mature Secondary Structure 
SLSFTTAIRGNFFDIAACVDQPQQLDQQLRHIPDGLMLLDEGHIVWFGPWQQGEPLLSP
CCEEEEHHCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCCCCCC
GFEVTHYQDKLIVPGFIDTHIHYPQTEMIGAYGDRLLAWLKHYTFPTESQFCCEQHSGKM
CCEEEEECCCEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCH
SAFFLQQLLSNGTTTALVFGTVHPQSVEALFSQAAALNMRLIAGKVMMDRHAPEELLETP
HHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCC
EQSYRQTRELIERWHGKQRLSYAITPRFAPTSTPALLAQVQRLREEFPDAYLHTHLSENL
HHHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
EEVAWVKELFPDSRSYLDVYHQYGLTGERSVFAHCLHLEEQEWDCLCHTGSAIAFCPTSN
HHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEECCCEEEECCCCC
LFLGSGLFNLQKAWQKQVRLGIGTDVGAGTTFNMLQTLGEAYKVAQLQRYQLSACEAFYH
CEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATLGGARALSLDDKIGNFSVGKEADFVVLDPAVSPLQKLRHENSRELAEQLFVLMTLGDD
HHCCCCEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCHHHHHHHHEEEEEECCC
RNVYRTYVDGKVVYRPATQQEAA
CCEEEEEECCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 10913105 [H]