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The map label for this gene is hsrA [H]

Identifier: 157370532

GI number: 157370532

Start: 2494477

End: 2495949

Strand: Reverse

Name: hsrA [H]

Synonym: Spro_2292

Alternate gene names: 157370532

Gene position: 2495949-2494477 (Counterclockwise)

Preceding gene: 157370533

Following gene: 157370531

Centisome position: 45.81

GC content: 60.15

Gene sequence:

>1473_bases
ATGCTGTTTTTACTCCGCCCGGCGCACTCAGCCATGAGTTTTTTTTCACGCGCCAGCGCGCTGCCGCTGCTGGTGGCGGG
TGCCTTCTTTATGGAAAACCTCGACGCCACGGTGATTGTCACCGCCATGCCGCAGATGGCGGCGGCCTTCGGCGTAAGGC
CGGTGGACATGAACATTGGTATCTCGGCTTATATTCTGACGCTGACGGTGTTTATTCCTGCCAGTGGCTGGATTGCCAAC
CGCTTCGGCGCCCGCAATATCTTTGCCGCCGCGGTGCTGATTTTTACCCTGGCATCGGTGCTGTGCGCCGCCAGCGTTAA
CCTGCCCACCTTCACCGCCGCCCGGATTCTGCAAGGCTTCGGGGGTGCGCTGATGGTGCCGGTGGGTCGCCTGGTGGTGC
TACGTAACACTGCCAAACCGGATCTGATCAAGGCCATTGCCACCATTACCTGGCCAGGGCTGGTAGCCCCAATCCTCGGC
CCGCCGGTCGGCGGTTTTATTACCACCTACGCCTCGTGGCACTGGATCTTTATTCTTAACGTGCCGCTGGGTATCGCCGC
GCTGTGGCTGGCCTGGCGGCTGATCCCGCAGGAAGCGTCGCAGAAAGGAGTGCCTTTTGACGCACTCGGCTTCGTATTGA
CCGGCATCGCCTGCTTCGGCCTGATGTTCGGTCTGGATTTGATCAACCACCCGCAACTGTCCTGGCTGGTGCCTGCACTG
TGTATTCTCGGCAGCCTGGCATTAGGCACACTGGCGGTTCGTCACGCCAAACGCACCGAGCACCCGCTGATCAATCTTTG
GGCGATGCGAATAAAAAGTTACGCCGTCACCATTTGGGGTGGCACGCTGTTCCGCATCGCCATTGGCGCCGTGCCCTTCC
TGCTGCCGCTGCTGTTCCAGATCGGTTTTGGCCTGAACGCCTTCGACGCCGGGCTGCTGGTACTGGCGGTTTTTGCCGGG
AATCTGGTGATGAAGCCGTTTACCTCGGCGGTGCTGTACCGTTTTCGCTTTCGCACCACGCTGGTGGTCAATGGTTTGTT
GAACGCGGCCACTATTTTCGCCTGCGCGCTGTTGACGCCACAGACCCCGACCTGGCTGATTCTGGCGCTGCTGTTCGTCA
GCGGGCTAACGCGGTCCATGCAGTTCACCGCGCTCAATACGCTGGCGTTTTCCGAAGTACCGCAGCCACAGATGAATGGT
GCCAACACGCTGTTCAATGTGTCGCAGCAAATGGGCAGCGGGCTGGGTATCGCCATCGGCGCACTGGCCCTGCGGCTGGC
AGAAATGTTGATGCCGCAATCGGGCGAGCGCATCCCCCTGGTCGATTTTCAGTGGGCATTTGTGGTCATCGGCGTGATTG
CCCTGCTGGCCGTGGTTGACTGCTTTACCCTGAACGCCAACGCCGGCAGCGAGGTTCGCCAGAGAAAACCGGCCGCCACG
GTGCCAAACTCCCCCCAACGATTGCAAAAATAA

Upstream 100 bases:

>100_bases
TATCACCTGCTTAACCTCTTTTTAACCTCTGTGTGATGCGCTATGCTCCAAAACTCTGCCAGCGTTAACATTTTTGAAAC
GAGGTTTCTGTCATGCCTGC

Downstream 100 bases:

>100_bases
GTCGCATCTGCCGCCAATCAACGTAATATCGATGATCCGCCCCGTTTCACCGGTGCGGATTTTTGCCGTTTTGGCCTGAA
AAGCGAGGTAATAATAATGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MLFLLRPAHSAMSFFSRASALPLLVAGAFFMENLDATVIVTAMPQMAAAFGVRPVDMNIGISAYILTLTVFIPASGWIAN
RFGARNIFAAAVLIFTLASVLCAASVNLPTFTAARILQGFGGALMVPVGRLVVLRNTAKPDLIKAIATITWPGLVAPILG
PPVGGFITTYASWHWIFILNVPLGIAALWLAWRLIPQEASQKGVPFDALGFVLTGIACFGLMFGLDLINHPQLSWLVPAL
CILGSLALGTLAVRHAKRTEHPLINLWAMRIKSYAVTIWGGTLFRIAIGAVPFLLPLLFQIGFGLNAFDAGLLVLAVFAG
NLVMKPFTSAVLYRFRFRTTLVVNGLLNAATIFACALLTPQTPTWLILALLFVSGLTRSMQFTALNTLAFSEVPQPQMNG
ANTLFNVSQQMGSGLGIAIGALALRLAEMLMPQSGERIPLVDFQWAFVVIGVIALLAVVDCFTLNANAGSEVRQRKPAAT
VPNSPQRLQK

Sequences:

>Translated_490_residues
MLFLLRPAHSAMSFFSRASALPLLVAGAFFMENLDATVIVTAMPQMAAAFGVRPVDMNIGISAYILTLTVFIPASGWIAN
RFGARNIFAAAVLIFTLASVLCAASVNLPTFTAARILQGFGGALMVPVGRLVVLRNTAKPDLIKAIATITWPGLVAPILG
PPVGGFITTYASWHWIFILNVPLGIAALWLAWRLIPQEASQKGVPFDALGFVLTGIACFGLMFGLDLINHPQLSWLVPAL
CILGSLALGTLAVRHAKRTEHPLINLWAMRIKSYAVTIWGGTLFRIAIGAVPFLLPLLFQIGFGLNAFDAGLLVLAVFAG
NLVMKPFTSAVLYRFRFRTTLVVNGLLNAATIFACALLTPQTPTWLILALLFVSGLTRSMQFTALNTLAFSEVPQPQMNG
ANTLFNVSQQMGSGLGIAIGALALRLAEMLMPQSGERIPLVDFQWAFVVIGVIALLAVVDCFTLNANAGSEVRQRKPAAT
VPNSPQRLQK
>Mature_490_residues
MLFLLRPAHSAMSFFSRASALPLLVAGAFFMENLDATVIVTAMPQMAAAFGVRPVDMNIGISAYILTLTVFIPASGWIAN
RFGARNIFAAAVLIFTLASVLCAASVNLPTFTAARILQGFGGALMVPVGRLVVLRNTAKPDLIKAIATITWPGLVAPILG
PPVGGFITTYASWHWIFILNVPLGIAALWLAWRLIPQEASQKGVPFDALGFVLTGIACFGLMFGLDLINHPQLSWLVPAL
CILGSLALGTLAVRHAKRTEHPLINLWAMRIKSYAVTIWGGTLFRIAIGAVPFLLPLLFQIGFGLNAFDAGLLVLAVFAG
NLVMKPFTSAVLYRFRFRTTLVVNGLLNAATIFACALLTPQTPTWLILALLFVSGLTRSMQFTALNTLAFSEVPQPQMNG
ANTLFNVSQQMGSGLGIAIGALALRLAEMLMPQSGERIPLVDFQWAFVVIGVIALLAVVDCFTLNANAGSEVRQRKPAAT
VPNSPQRLQK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=471, Percent_Identity=35.6687898089172, Blast_Score=300, Evalue=2e-82,
Organism=Escherichia coli, GI1788392, Length=461, Percent_Identity=33.8394793926247, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1788710, Length=398, Percent_Identity=25.3768844221106, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI87081983, Length=398, Percent_Identity=25.6281407035176, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789042, Length=401, Percent_Identity=25.4364089775561, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6325455, Length=175, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52553; Mature: 52553

Theoretical pI: Translated: 10.98; Mature: 10.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFLLRPAHSAMSFFSRASALPLLVAGAFFMENLDATVIVTAMPQMAAAFGVRPVDMNIG
CEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCEEECCCC
ISAYILTLTVFIPASGWIANRFGARNIFAAAVLIFTLASVLCAASVNLPTFTAARILQGF
HHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
GGALMVPVGRLVVLRNTAKPDLIKAIATITWPGLVAPILGPPVGGFITTYASWHWIFILN
CCEEECCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCEEEEEEEE
VPLGIAALWLAWRLIPQEASQKGVPFDALGFVLTGIACFGLMFGLDLINHPQLSWLVPAL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CILGSLALGTLAVRHAKRTEHPLINLWAMRIKSYAVTIWGGTLFRIAIGAVPFLLPLLFQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHH
IGFGLNAFDAGLLVLAVFAGNLVMKPFTSAVLYRFRFRTTLVVNGLLNAATIFACALLTP
HCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTPTWLILALLFVSGLTRSMQFTALNTLAFSEVPQPQMNGANTLFNVSQQMGSGLGIAIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ALALRLAEMLMPQSGERIPLVDFQWAFVVIGVIALLAVVDCFTLNANAGSEVRQRKPAAT
HHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCC
VPNSPQRLQK
CCCCHHHHCC
>Mature Secondary Structure
MLFLLRPAHSAMSFFSRASALPLLVAGAFFMENLDATVIVTAMPQMAAAFGVRPVDMNIG
CEEEEECHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCEEECCCC
ISAYILTLTVFIPASGWIANRFGARNIFAAAVLIFTLASVLCAASVNLPTFTAARILQGF
HHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
GGALMVPVGRLVVLRNTAKPDLIKAIATITWPGLVAPILGPPVGGFITTYASWHWIFILN
CCEEECCCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHCCEEEEEEEE
VPLGIAALWLAWRLIPQEASQKGVPFDALGFVLTGIACFGLMFGLDLINHPQLSWLVPAL
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CILGSLALGTLAVRHAKRTEHPLINLWAMRIKSYAVTIWGGTLFRIAIGAVPFLLPLLFQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHHHHHH
IGFGLNAFDAGLLVLAVFAGNLVMKPFTSAVLYRFRFRTTLVVNGLLNAATIFACALLTP
HCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QTPTWLILALLFVSGLTRSMQFTALNTLAFSEVPQPQMNGANTLFNVSQQMGSGLGIAIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHH
ALALRLAEMLMPQSGERIPLVDFQWAFVVIGVIALLAVVDCFTLNANAGSEVRQRKPAAT
HHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCC
VPNSPQRLQK
CCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]