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The map label for this gene is ulaC [H]

Identifier: 157370493

GI number: 157370493

Start: 2455357

End: 2455794

Strand: Reverse

Name: ulaC [H]

Synonym: Spro_2253

Alternate gene names: 157370493

Gene position: 2455794-2455357 (Counterclockwise)

Preceding gene: 157370494

Following gene: 157370492

Centisome position: 45.07

GC content: 52.28

Gene sequence:

>438_bases
ATGACCATTAAACAACTGTTGATCGAGGCCGGAGCAATACAGGTCGGCGTGCAGGAAACGGACTGGAAAAAGGTGATTGA
GTTGGCGGCACAGCCTTTGGTCGAGAACGGCTATATTCACCCTTCCTATCACCAAGCGGTGATCAGTAATACGCTGGCCC
ATGGCGCTTATTATGTGTTTGACGAAGGCATTGCCATCCCCCATGCCCGGCCGGAATGCGGCGTGATTAAAGATTGTTTC
AGCATGGTGCTGCTGGATCAGCCTGTCGCCTTTCAGGACAGTGAAAAGGCCGATATTGTCATCCTGTTCGGCGCAACGGA
CAGCAACCGGCATATTGAAGAAGGCATCCGGGCGATTGTCGAGTTACTGGACAACAAACAGCGGCTCGATAAACTGCGCG
CCGCCAAACTCTGGCAAGAGGTGGTAGAAATCTTATGA

Upstream 100 bases:

>100_bases
TTATGCCAAAAATAAAAATCACCGCCAGGAAATCGATGTGGTTTTTATCAAAATGGACGAGCTGCGCAATATTATCAGGA
AGTCTCTTTGAGGGAACATG

Downstream 100 bases:

>100_bases
CTGCGCTAACTGACAGCAGCGACAAAACCGTTATTTTGGTCAATGGCATTCCGGCTTCTGGCAAGAGCACCCTCGCCCGT
GCGCTGGCGCAGCACTTTGG

Product: putative PTS IIA-like nitrogen-regulatory protein PtsN

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 145; Mature: 144

Protein sequence:

>145_residues
MTIKQLLIEAGAIQVGVQETDWKKVIELAAQPLVENGYIHPSYHQAVISNTLAHGAYYVFDEGIAIPHARPECGVIKDCF
SMVLLDQPVAFQDSEKADIVILFGATDSNRHIEEGIRAIVELLDNKQRLDKLRAAKLWQEVVEIL

Sequences:

>Translated_145_residues
MTIKQLLIEAGAIQVGVQETDWKKVIELAAQPLVENGYIHPSYHQAVISNTLAHGAYYVFDEGIAIPHARPECGVIKDCF
SMVLLDQPVAFQDSEKADIVILFGATDSNRHIEEGIRAIVELLDNKQRLDKLRAAKLWQEVVEIL
>Mature_144_residues
TIKQLLIEAGAIQVGVQETDWKKVIELAAQPLVENGYIHPSYHQAVISNTLAHGAYYVFDEGIAIPHARPECGVIKDCFS
MVLLDQPVAFQDSEKADIVILFGATDSNRHIEEGIRAIVELLDNKQRLDKLRAAKLWQEVVEIL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=147, Percent_Identity=34.6938775510204, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI1790755, Length=126, Percent_Identity=39.6825396825397, Blast_Score=92, Evalue=1e-20,
Organism=Escherichia coli, GI1789302, Length=93, Percent_Identity=38.7096774193548, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16206; Mature: 16075

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKQLLIEAGAIQVGVQETDWKKVIELAAQPLVENGYIHPSYHQAVISNTLAHGAYYVF
CCHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCEEEEE
DEGIAIPHARPECGVIKDCFSMVLLDQPVAFQDSEKADIVILFGATDSNRHIEEGIRAIV
ECCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
ELLDNKQRLDKLRAAKLWQEVVEIL
HHHHCHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TIKQLLIEAGAIQVGVQETDWKKVIELAAQPLVENGYIHPSYHQAVISNTLAHGAYYVF
CHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCEEEEE
DEGIAIPHARPECGVIKDCFSMVLLDQPVAFQDSEKADIVILFGATDSNRHIEEGIRAIV
ECCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
ELLDNKQRLDKLRAAKLWQEVVEIL
HHHHCHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]