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Identifier: 157370491

GI number: 157370491

Start: 2453576

End: 2454574

Strand: Reverse

Name: add

Synonym: Spro_2251

Alternate gene names: 157370491

Gene position: 2454574-2453576 (Counterclockwise)

Preceding gene: 157370492

Following gene: 157370484

Centisome position: 45.05

GC content: 60.86

Gene sequence:

>999_bases
ATGATTGACTCCCGCCTTCCGCTGACCGACATCCACCGTCACCTCGACGGTAACATTCGTGCGCAAACCATCCTCGACCT
GGGCCGTCAGTTTAACCTTGCCCTGCCAGCCGACGAACTGGAAGCCCTGCGTCCGCACGTGCAAATCACCCATGCCGAAC
CGGATCTGGTCAGCTTCTTGCAAAAGCTCGACTGGGGAGTGGCGGTGCTGGGGGATTTGGAAGCCTGCCGCCGGGTCGCC
TACGAAAACGTTGAGGACGCCGCTAACGCCGGTCTGCACTACGCCGAACTGCGTTTCTCGCCGTATTACATGGCGATGAA
GCACCAGTTACCGGTAACTGGGGTGGTAGAGGCGGTGATTGATGGCATTCGTTCCGGCTCCCGCGATTTGGGCATTGATG
TGCGCCTGATCGGCATCATGAGCCGTACCTTCGGCGAGGCCGCCTGCTTGCAAGAGCTGGAAGGTCTGCTGGCACACCGT
GACGGTATTACCGCGCTGGATCTGGCCGGTGACGAACTCGGCTTCCCGGGTGGTCTGTTCCTCAACCACTTTAACCGCGC
CCGTGACGCCGGGTTGCGTATCACCGTGCACGCCGGTGAAGCCGCCGGCCCGGAAAGCATCTGGCAGGCGATCCGTGAGT
TGGGCGCAGAGCGTATCGGCCACGGGGTGAAAGCCGTTGAAGATCCGGCATTAATGGACTTCCTGGCGGAACACGGCATT
GGTATCGAGTCTTGCCTGACCTCCAACATTCAGACCAGCACCGTGGCTTCTCTGGCTCAGCATCCGCTGGCGAAGTTCCT
GCGTCACGGCGTCATGGCGTCGATCAACACCGACGATCCGGCGGTTCAGGGCATAGAAATTGAGCACGAATACCTGGTAG
CGGCACCACAGGCCGGGCTGACGCCGGCGGAGATCCGCACCGCTCAGGAAAACGGCCTGAAAATGGCGTTCCTTAGCGAG
CAGGAAAAGCAGACGCTGCGCGATAAAGTCCGCGGCTGA

Upstream 100 bases:

>100_bases
TGATTGCAGTCACTTTTTTATGCAACGACATGGCAATTTTCATCATTTTGTTTTTATACTGATCTGCACTTTACCTACAA
CAAAATAACGAGTGCACATC

Downstream 100 bases:

>100_bases
TTGAATGGAATAACGGGCGCTCTTACGCGTAATACTTACCAGTTGAGGCGATATTGTTCCCTCTCCTTTGGGTGAGGGGG
ACGCCCACTTAAGCCACGAC

Product: adenosine deaminase

Products: NA

Alternate protein names: Adenosine aminohydrolase

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MIDSRLPLTDIHRHLDGNIRAQTILDLGRQFNLALPADELEALRPHVQITHAEPDLVSFLQKLDWGVAVLGDLEACRRVA
YENVEDAANAGLHYAELRFSPYYMAMKHQLPVTGVVEAVIDGIRSGSRDLGIDVRLIGIMSRTFGEAACLQELEGLLAHR
DGITALDLAGDELGFPGGLFLNHFNRARDAGLRITVHAGEAAGPESIWQAIRELGAERIGHGVKAVEDPALMDFLAEHGI
GIESCLTSNIQTSTVASLAQHPLAKFLRHGVMASINTDDPAVQGIEIEHEYLVAAPQAGLTPAEIRTAQENGLKMAFLSE
QEKQTLRDKVRG

Sequences:

>Translated_332_residues
MIDSRLPLTDIHRHLDGNIRAQTILDLGRQFNLALPADELEALRPHVQITHAEPDLVSFLQKLDWGVAVLGDLEACRRVA
YENVEDAANAGLHYAELRFSPYYMAMKHQLPVTGVVEAVIDGIRSGSRDLGIDVRLIGIMSRTFGEAACLQELEGLLAHR
DGITALDLAGDELGFPGGLFLNHFNRARDAGLRITVHAGEAAGPESIWQAIRELGAERIGHGVKAVEDPALMDFLAEHGI
GIESCLTSNIQTSTVASLAQHPLAKFLRHGVMASINTDDPAVQGIEIEHEYLVAAPQAGLTPAEIRTAQENGLKMAFLSE
QEKQTLRDKVRG
>Mature_332_residues
MIDSRLPLTDIHRHLDGNIRAQTILDLGRQFNLALPADELEALRPHVQITHAEPDLVSFLQKLDWGVAVLGDLEACRRVA
YENVEDAANAGLHYAELRFSPYYMAMKHQLPVTGVVEAVIDGIRSGSRDLGIDVRLIGIMSRTFGEAACLQELEGLLAHR
DGITALDLAGDELGFPGGLFLNHFNRARDAGLRITVHAGEAAGPESIWQAIRELGAERIGHGVKAVEDPALMDFLAEHGI
GIESCLTSNIQTSTVASLAQHPLAKFLRHGVMASINTDDPAVQGIEIEHEYLVAAPQAGLTPAEIRTAQENGLKMAFLSE
QEKQTLRDKVRG

Specific function: Unknown

COG id: COG1816

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenosine and AMP deaminases family

Homologues:

Organism=Homo sapiens, GI47078295, Length=339, Percent_Identity=32.1533923303835, Blast_Score=151, Evalue=7e-37,
Organism=Escherichia coli, GI1787910, Length=330, Percent_Identity=77.5757575757576, Blast_Score=525, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI17538512, Length=346, Percent_Identity=32.0809248554913, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32565969, Length=346, Percent_Identity=31.7919075144509, Blast_Score=135, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI115537133, Length=322, Percent_Identity=25.776397515528, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6324188, Length=283, Percent_Identity=27.208480565371, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24645260, Length=334, Percent_Identity=26.9461077844311, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ADD_SERP5 (A8GE12)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478480.1
- ProteinModelPortal:   A8GE12
- SMR:   A8GE12
- STRING:   A8GE12
- GeneID:   5605091
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2251
- eggNOG:   COG1816
- HOGENOM:   HBG630382
- OMA:   PYYMAMN
- ProtClustDB:   PRK09358
- BioCyc:   SPRO399741:SPRO_2251-MONOMER
- HAMAP:   MF_00540
- InterPro:   IPR006650
- InterPro:   IPR001365
- InterPro:   IPR006330
- PANTHER:   PTHR11409
- TIGRFAMs:   TIGR01430

Pfam domain/function: PF00962 A_deaminase

EC number: =3.5.4.4

Molecular weight: Translated: 36132; Mature: 36132

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00485 A_DEAMINASE

Important sites: ACT_SITE 200-200 BINDING 14-14 BINDING 16-16 BINDING 170-170 BINDING 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSRLPLTDIHRHLDGNIRAQTILDLGRQFNLALPADELEALRPHVQITHAEPDLVSFL
CCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHH
QKLDWGVAVLGDLEACRRVAYENVEDAANAGLHYAELRFSPYYMAMKHQLPVTGVVEAVI
HHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHHHHHHH
DGIRSGSRDLGIDVRLIGIMSRTFGEAACLQELEGLLAHRDGITALDLAGDELGFPGGLF
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHH
LNHFNRARDAGLRITVHAGEAAGPESIWQAIRELGAERIGHGVKAVEDPALMDFLAEHGI
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCC
GIESCLTSNIQTSTVASLAQHPLAKFLRHGVMASINTDDPAVQGIEIEHEYLVAAPQAGL
CHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCEEEECCCCCC
TPAEIRTAQENGLKMAFLSEQEKQTLRDKVRG
CHHHHHHHHHCCCEEEECCHHHHHHHHHHHCC
>Mature Secondary Structure
MIDSRLPLTDIHRHLDGNIRAQTILDLGRQFNLALPADELEALRPHVQITHAEPDLVSFL
CCCCCCCHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEECCCCHHHHHH
QKLDWGVAVLGDLEACRRVAYENVEDAANAGLHYAELRFSPYYMAMKHQLPVTGVVEAVI
HHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCEEEEEECCHHHHHHHHCCCHHHHHHHHH
DGIRSGSRDLGIDVRLIGIMSRTFGEAACLQELEGLLAHRDGITALDLAGDELGFPGGLF
HHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHH
LNHFNRARDAGLRITVHAGEAAGPESIWQAIRELGAERIGHGVKAVEDPALMDFLAEHGI
HHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCC
GIESCLTSNIQTSTVASLAQHPLAKFLRHGVMASINTDDPAVQGIEIEHEYLVAAPQAGL
CHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEECCCEEEECCCCCC
TPAEIRTAQENGLKMAFLSEQEKQTLRDKVRG
CHHHHHHHHHCCCEEEECCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA