Click here to switch to the map view.

The map label for this gene is eptA [H]

Identifier: 157370444

GI number: 157370444

Start: 2405171

End: 2406808

Strand: Reverse

Name: eptA [H]

Synonym: Spro_2204

Alternate gene names: 157370444

Gene position: 2406808-2405171 (Counterclockwise)

Preceding gene: 157370445

Following gene: 157370443

Centisome position: 44.17

GC content: 53.54

Gene sequence:

>1638_bases
ATGTCGTTACGTCAACGCTTACAGTGCAACAGCCTGGGTTTTATCCTCGCCAGTGCCTTATTCTTCACTCTGTTCCAGAA
TGCGTTATTCCTTCATAAGGCCTGGTCGTATATCACCTTCGATAACCTCCACAGCGTGATTTTCGCCGCCACCATGCCGG
TGGTGATTTTCTGTGCACTGAATATTATTTTCAGCGTGCTGACCGTGCCGTTCTTGCGCAAGCCACTGATGATTTTCTTC
CTGCTCGGCAGCGCGGCCGCCAACTACTTTATGTTCAGTTACGGCGTGGTGATCGACGGCAATATGATGCAGAACGCCTT
CGAAACCAATCCCCAGGAAGCAACGGCGCTGTTGACGCCACGCATGGGTCTGTGGCTGTTCCTGCTGGGTATTCTGCCTG
CGGTATTGGTTTGCTTTATCGAAATCCGTAAAACTCGCCCATGGTGGTACATGGTCGGGTTGCGTGCCGCTAACGTGATG
CTCTCGGTAGTGGTGATCCTGATTATCGCCGCCCTGTTTTATAAGGATTACGCCTCGCTGATCCGCAATAACAAAAGCGT
GGTGAAAATGCTGACGCCGTCGAACTTCGTTTCCGGCACCATCAAATTTACCCAGCAACGTTACTTCACCCGCAATTTGC
CGCTGGTGAAAATCGGTGAAGATGCACGTAAAGGGCCAGTAATCACCGCACAGCAAAAGAAAACCCTGGTGATCCTGGTG
GTCGGGGAAACGGCGCGTGCCGAGAACTTCTCGTTGGGCGGCTATGAGCGCGAAACCAATCCGCGTCTGAAGCAGGACAA
CGTGGTGTATTTTAAAAACGCCAGCTCCTGCGGCACCGAAACGGCGATCTCCGTGCCTTGCATGTTCTCCAACATGCCAC
GCAAGGATTATGACGCCACCCAGGCTACCCATCAGGAGGGCATGCTTGACGTACTGGCGCATGCCGGTGTCAATGTGTTG
TGGCGTGAAAACGACGGCGGATGCAAAGGTGCCTGCGATCGCGTGCCCCACGTTGACATGACCAAGCTAAAACTGCCGCA
GGATTGCGACGGTGAAGTCTGCATGGATAACGTGCTGCTGTATAAACTTAACGACTACATCAACAGCCTGAAAGACGACG
GCGTGATAGTGCTGCACCAGATGGGCAGCCACGGGCCGGCCTATTTCCGTCGCAGTACGCCAGAGTTCCAGAAGTTCTCA
CCCACCTGCAACAGTAATCAGATCCAGGATTGCAGCCACGAGCAGTTGGTGAATACCTATGACAACTCACTGCTGTATAC
CGACGCCATGCTGGACGACACCATCAAGCTATTGCAACAGCACAGCGACAAGTTCAACACCGCGCTGGTGTATCTCTCTG
ACCACGGTGAATCACTGGGCGAAAACGGCATGTATCTGCACGGTACTCCTTACGTGTTTGCCCCAAGCCAGCAGACCCAT
GTCCCCTTCCTGATGTGGATGTCTGCGGACTATGAGCGCAACTTCAAGGTTGATCGCCAGTGCCTGAGTACCATGGCAGA
AAAGGATGATATCTCGCAGGACAACCTGTTCCACACCATGCTCGGCATGCTGAACGTGCAAACCCGTGAATATCAACCGC
AGCTCGATATTCTGCAACGCTGCCGGGGAGCCGCCTAA

Upstream 100 bases:

>100_bases
AATTAAACAACTCTGCCACCAAACTGAATCTCTAAGGTTTTCTTAAGTTTCATCCTGTAAATTTCAGCGCATTACACAAT
ATCAGGATTCAGATTTGGCA

Downstream 100 bases:

>100_bases
GGGCTTAACTTCCGCACTTTATTCAGGCAAAGTATCACTCATCGTAACCGGCATTTTTATGCCGGTTTTTTTATTGTTAT
TGACCTAGACCAATCGGTCT

Product: putative cell division protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSLRQRLQCNSLGFILASALFFTLFQNALFLHKAWSYITFDNLHSVIFAATMPVVIFCALNIIFSVLTVPFLRKPLMIFF
LLGSAAANYFMFSYGVVIDGNMMQNAFETNPQEATALLTPRMGLWLFLLGILPAVLVCFIEIRKTRPWWYMVGLRAANVM
LSVVVILIIAALFYKDYASLIRNNKSVVKMLTPSNFVSGTIKFTQQRYFTRNLPLVKIGEDARKGPVITAQQKKTLVILV
VGETARAENFSLGGYERETNPRLKQDNVVYFKNASSCGTETAISVPCMFSNMPRKDYDATQATHQEGMLDVLAHAGVNVL
WRENDGGCKGACDRVPHVDMTKLKLPQDCDGEVCMDNVLLYKLNDYINSLKDDGVIVLHQMGSHGPAYFRRSTPEFQKFS
PTCNSNQIQDCSHEQLVNTYDNSLLYTDAMLDDTIKLLQQHSDKFNTALVYLSDHGESLGENGMYLHGTPYVFAPSQQTH
VPFLMWMSADYERNFKVDRQCLSTMAEKDDISQDNLFHTMLGMLNVQTREYQPQLDILQRCRGAA

Sequences:

>Translated_545_residues
MSLRQRLQCNSLGFILASALFFTLFQNALFLHKAWSYITFDNLHSVIFAATMPVVIFCALNIIFSVLTVPFLRKPLMIFF
LLGSAAANYFMFSYGVVIDGNMMQNAFETNPQEATALLTPRMGLWLFLLGILPAVLVCFIEIRKTRPWWYMVGLRAANVM
LSVVVILIIAALFYKDYASLIRNNKSVVKMLTPSNFVSGTIKFTQQRYFTRNLPLVKIGEDARKGPVITAQQKKTLVILV
VGETARAENFSLGGYERETNPRLKQDNVVYFKNASSCGTETAISVPCMFSNMPRKDYDATQATHQEGMLDVLAHAGVNVL
WRENDGGCKGACDRVPHVDMTKLKLPQDCDGEVCMDNVLLYKLNDYINSLKDDGVIVLHQMGSHGPAYFRRSTPEFQKFS
PTCNSNQIQDCSHEQLVNTYDNSLLYTDAMLDDTIKLLQQHSDKFNTALVYLSDHGESLGENGMYLHGTPYVFAPSQQTH
VPFLMWMSADYERNFKVDRQCLSTMAEKDDISQDNLFHTMLGMLNVQTREYQPQLDILQRCRGAA
>Mature_544_residues
SLRQRLQCNSLGFILASALFFTLFQNALFLHKAWSYITFDNLHSVIFAATMPVVIFCALNIIFSVLTVPFLRKPLMIFFL
LGSAAANYFMFSYGVVIDGNMMQNAFETNPQEATALLTPRMGLWLFLLGILPAVLVCFIEIRKTRPWWYMVGLRAANVML
SVVVILIIAALFYKDYASLIRNNKSVVKMLTPSNFVSGTIKFTQQRYFTRNLPLVKIGEDARKGPVITAQQKKTLVILVV
GETARAENFSLGGYERETNPRLKQDNVVYFKNASSCGTETAISVPCMFSNMPRKDYDATQATHQEGMLDVLAHAGVNVLW
RENDGGCKGACDRVPHVDMTKLKLPQDCDGEVCMDNVLLYKLNDYINSLKDDGVIVLHQMGSHGPAYFRRSTPEFQKFSP
TCNSNQIQDCSHEQLVNTYDNSLLYTDAMLDDTIKLLQQHSDKFNTALVYLSDHGESLGENGMYLHGTPYVFAPSQQTHV
PFLMWMSADYERNFKVDRQCLSTMAEKDDISQDNLFHTMLGMLNVQTREYQPQLDILQRCRGAA

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=524, Percent_Identity=53.0534351145038, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI87082286, Length=554, Percent_Identity=24.0072202166065, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1790392, Length=576, Percent_Identity=23.4375, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082223, Length=458, Percent_Identity=24.0174672489083, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 61657; Mature: 61526

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRQRLQCNSLGFILASALFFTLFQNALFLHKAWSYITFDNLHSVIFAATMPVVIFCAL
CCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
NIIFSVLTVPFLRKPLMIFFLLGSAAANYFMFSYGVVIDGNMMQNAFETNPQEATALLTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECEEEECHHHHHHHHCCHHHHHEECCC
RMGLWLFLLGILPAVLVCFIEIRKTRPWWYMVGLRAANVMLSVVVILIIAALFYKDYASL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRNNKSVVKMLTPSNFVSGTIKFTQQRYFTRNLPLVKIGEDARKGPVITAQQKKTLVILV
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCEEEEEE
VGETARAENFSLGGYERETNPRLKQDNVVYFKNASSCGTETAISVPCMFSNMPRKDYDAT
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCHHHCCCCCCCCCHH
QATHQEGMLDVLAHAGVNVLWRENDGGCKGACDRVPHVDMTKLKLPQDCDGEVCMDNVLL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCHHHCCCCCCCCCCHHHHHHHH
YKLNDYINSLKDDGVIVLHQMGSHGPAYFRRSTPEFQKFSPTCNSNQIQDCSHEQLVNTY
HHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
DNSLLYTDAMLDDTIKLLQQHSDKFNTALVYLSDHGESLGENGMYLHGTPYVFAPSQQTH
CCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCCCCC
VPFLMWMSADYERNFKVDRQCLSTMAEKDDISQDNLFHTMLGMLNVQTREYQPQLDILQR
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHH
CRGAA
HCCCC
>Mature Secondary Structure 
SLRQRLQCNSLGFILASALFFTLFQNALFLHKAWSYITFDNLHSVIFAATMPVVIFCAL
CHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
NIIFSVLTVPFLRKPLMIFFLLGSAAANYFMFSYGVVIDGNMMQNAFETNPQEATALLTP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECEEEECHHHHHHHHCCHHHHHEECCC
RMGLWLFLLGILPAVLVCFIEIRKTRPWWYMVGLRAANVMLSVVVILIIAALFYKDYASL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IRNNKSVVKMLTPSNFVSGTIKFTQQRYFTRNLPLVKIGEDARKGPVITAQQKKTLVILV
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEECCCCEEEEEE
VGETARAENFSLGGYERETNPRLKQDNVVYFKNASSCGTETAISVPCMFSNMPRKDYDAT
ECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEECCHHHCCCCCCCCCHH
QATHQEGMLDVLAHAGVNVLWRENDGGCKGACDRVPHVDMTKLKLPQDCDGEVCMDNVLL
HHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHCCCCCCCHHHCCCCCCCCCCHHHHHHHH
YKLNDYINSLKDDGVIVLHQMGSHGPAYFRRSTPEFQKFSPTCNSNQIQDCSHEQLVNTY
HHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHH
DNSLLYTDAMLDDTIKLLQQHSDKFNTALVYLSDHGESLGENGMYLHGTPYVFAPSQQTH
CCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEECCCEEECCCCCCC
VPFLMWMSADYERNFKVDRQCLSTMAEKDDISQDNLFHTMLGMLNVQTREYQPQLDILQR
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHCCCCHHHHHHH
CRGAA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]