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The map label for this gene is ribE [H]

Identifier: 157370430

GI number: 157370430

Start: 2392416

End: 2393066

Strand: Direct

Name: ribE [H]

Synonym: Spro_2190

Alternate gene names: 157370430

Gene position: 2392416-2393066 (Clockwise)

Preceding gene: 157370425

Following gene: 157370431

Centisome position: 43.91

GC content: 52.84

Gene sequence:

>651_bases
ATGTTCACCGGTATCGTACAAGGCACCGCGCCGCTGGTCGCCATTGATGAAAAATCCAACTTCCGTACTCATGTCATTGA
GATGCCCACTGAACTGCTGCCTGGGCTGGAGCTTGGCGCGTCGGTATCTCATAACGGTTGCTGTCTGACCGTCACGGCGG
TAGAGGGCAACCGCGTCAGTTTTGATTTGATCAAAGAAACGCTGCGCCTGACCAACCTCGGTGAGCTGGCATTGGGTGAC
GTGGTCAACATCGAAAGAGCGGCCAAATTTAATGATGAAATTGGCGGTCACCTGATGTCCGGCCATATTATCTGCACGGC
AGAGGTGGCCAAGATTTATACCTCTGAGAATAATCGTCAGGTGTGGTTGCGCATGCCGGATGCGGAACTGATGAAATACG
TTCTGCACAAAGGTTTCATTGGCATCGATGGTATTAGTCTGACTATCGGTGAGGTAGTCAATAACCGTTTTTGCGTACAT
CTGATCCCGGAAACCCTGGAGCGCACCACGCTGGGTAAAAAACGTCTGGGTGATAAGGTGAATATTGAGATCGATCCACA
AACGCAGGCGGTGGTTGATACTGTCGAGCGGGTGTTGGCGAGCCGTGAAGCGGCAATGGCGGCGGCGAGTGCGCTGGCGC
AGAAGATCTGA

Upstream 100 bases:

>100_bases
CGCAGCCGCAGATTTTTTGTCGCCTCGGGTTTGGTTTTCCTCGTCAACTGGGGCAAACTGCCCCAAGCGCTTTAAACATT
TTTAGAAAGAAGAGGCCCAC

Downstream 100 bases:

>100_bases
CGAACGACAAACCAGAGAGGAGCAATCGCGTGAAGTTTTATGGGAAAACGTTGATGTTAACGGCGCTGCTGGCGCTGAGT
GCTTGTAGCAGCTCGCCTGA

Product: riboflavin synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MFTGIVQGTAPLVAIDEKSNFRTHVIEMPTELLPGLELGASVSHNGCCLTVTAVEGNRVSFDLIKETLRLTNLGELALGD
VVNIERAAKFNDEIGGHLMSGHIICTAEVAKIYTSENNRQVWLRMPDAELMKYVLHKGFIGIDGISLTIGEVVNNRFCVH
LIPETLERTTLGKKRLGDKVNIEIDPQTQAVVDTVERVLASREAAMAAASALAQKI

Sequences:

>Translated_216_residues
MFTGIVQGTAPLVAIDEKSNFRTHVIEMPTELLPGLELGASVSHNGCCLTVTAVEGNRVSFDLIKETLRLTNLGELALGD
VVNIERAAKFNDEIGGHLMSGHIICTAEVAKIYTSENNRQVWLRMPDAELMKYVLHKGFIGIDGISLTIGEVVNNRFCVH
LIPETLERTTLGKKRLGDKVNIEIDPQTQAVVDTVERVLASREAAMAAASALAQKI
>Mature_216_residues
MFTGIVQGTAPLVAIDEKSNFRTHVIEMPTELLPGLELGASVSHNGCCLTVTAVEGNRVSFDLIKETLRLTNLGELALGD
VVNIERAAKFNDEIGGHLMSGHIICTAEVAKIYTSENNRQVWLRMPDAELMKYVLHKGFIGIDGISLTIGEVVNNRFCVH
LIPETLERTTLGKKRLGDKVNIEIDPQTQAVVDTVERVLASREAAMAAASALAQKI

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The alpha subunit catalyze

COG id: COG0307

COG function: function code H; Riboflavin synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lumazine-binding repeats [H]

Homologues:

Organism=Escherichia coli, GI1787952, Length=206, Percent_Identity=78.6407766990291, Blast_Score=346, Evalue=6e-97,
Organism=Saccharomyces cerevisiae, GI6319733, Length=195, Percent_Identity=32.3076923076923, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001783
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00677 Lum_binding [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 23506; Mature: 23506

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00693 LUM_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTGIVQGTAPLVAIDEKSNFRTHVIEMPTELLPGLELGASVSHNGCCLTVTAVEGNRVS
CCCCEECCCCCEEEEECCCCCEEEEEECCHHHCCCCCCCCCCCCCCEEEEEEEECCCEEE
FDLIKETLRLTNLGELALGDVVNIERAAKFNDEIGGHLMSGHIICTAEVAKIYTSENNRQ
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHCCCEEECCEEEEEEEEEEEEECCCCCE
VWLRMPDAELMKYVLHKGFIGIDGISLTIGEVVNNRFCVHLIPETLERTTLGKKRLGDKV
EEEECCCHHHHHHHHHCCCEECCCCEEEHHHHHCCCEEEEECHHHHHHHHCCHHHCCCEE
NIEIDPQTQAVVDTVERVLASREAAMAAASALAQKI
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFTGIVQGTAPLVAIDEKSNFRTHVIEMPTELLPGLELGASVSHNGCCLTVTAVEGNRVS
CCCCEECCCCCEEEEECCCCCEEEEEECCHHHCCCCCCCCCCCCCCEEEEEEEECCCEEE
FDLIKETLRLTNLGELALGDVVNIERAAKFNDEIGGHLMSGHIICTAEVAKIYTSENNRQ
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHCCHHCCCEEECCEEEEEEEEEEEEECCCCCE
VWLRMPDAELMKYVLHKGFIGIDGISLTIGEVVNNRFCVHLIPETLERTTLGKKRLGDKV
EEEECCCHHHHHHHHHCCCEECCCCEEEHHHHHCCCEEEEECHHHHHHHHCCHHHCCCEE
NIEIDPQTQAVVDTVERVLASREAAMAAASALAQKI
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9022701; 9023191; 9097039; 9278503; 11377200 [H]