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The map label for this gene is mdtK [H]

Identifier: 157370429

GI number: 157370429

Start: 2390797

End: 2392173

Strand: Reverse

Name: mdtK [H]

Synonym: Spro_2189

Alternate gene names: 157370429

Gene position: 2392173-2390797 (Counterclockwise)

Preceding gene: 157370432

Following gene: 157370428

Centisome position: 43.9

GC content: 58.02

Gene sequence:

>1377_bases
GTGCAGAAGTACTTAATTGAAGCGCGTAGCTTATTGGCCCTCGCAATCCCGGTGATCATCGCGCAAATATCACAAACCGC
AATGGGTGTGGTTGATACCATTATGGCCGGTGCCTACAGTGCCACAGATATGGCGGCGGTTGCCGTTGGTACCTCAATCT
GGCTACCGGCTATCCTGTTCGGCCACGGCCTGCTGCTGGCGTTAACGCCGGTGATTGCACAGCTGAACGGCGCCGGCCGA
CGCGATCGCATCGCTCATCAGGTGCGTCAGGGCTTTTGGCTGGCGGCCGCCGTCTCACTGCTGGTGATTATCGTGCTGTA
TAACAGCAAGTTTGTCATCGACATGATGCACAACATCGACCCGCAGTTGGCGGATAAAGCGGTAGGCTATCTGCATGCCA
TTATGTGGGGCGCGCCGGGTTATCTGTTCTTCCAGGTGCTGCGTGGCCAATGCGAAGGCCTGTCGAAAACCAAACCGGGC
ATGGTGATTGGTTTTCTCGGCCTGCTGGTGAATATCCCGATTAACTATATTTTCATCTACGGTAAATTCGGCATGCCGGA
ACTGGGCGGCGTCGGTTGCGGCGTGGCAACCGGCAGCGTCTACTGGTTTATGTTCCTGGCGATGCGCTGGTACGTGAAAC
ATGCCCCATCACAGCGTGACATTCTGCCACAGGAAGGCAGCAGCATGCGGCCGGACTGGGCGGCGATGAAACGCCTGTTC
GGCATTGGCCTGCCGGTGGCACTGGCGCTGTTCTTTGAAGTCACGCTGTTCGCCGTCGTCGCCCTGCTGGTGTCGCCACT
GGGCATTGTTTCCGTGGCCGGGCACCAGATTGCACTTAACTTCAGCGCACTGATGTTTGTGTTGCCATTGTCACTGGGCG
TCAGCGCGACTATCCGCGTCGGCTATAGATTGGGCGAAGGCTCGGTCGAAGGCGCGCGGGTTGCCGCTTATTCGGCTATC
GGCGTCGGTATTCTGATGGCCTGCTGCACCGCGTTGTTCACCGCCACCTTCCGCGAGCCAATCGCCCTGCTGTACAACGA
CAGCCCGGCGGTAGTTGCCATGGCCTCGCAGCTGATGCTGCTGGCGGCGATTTACCAGATCTCCGATTCCATTCAGGTGA
TTGGCAGCGGTGTCCTGCGCGGTTATAAAGACACCCGTTCGATCTTCTTCATCACCTTTATCGCCTATTGGATCCTGGGG
CTGCCGAGCGGTTATGTGCTGGCGCTGACCGATATTCTGGTACCGGCAATGGGGCCAAGCGGATTCTGGATTGGCTTTAT
CATTGGCCTGACCTTCGCCGCCATCATGATGGCCATGCGCATGCGTTGGCTGCAAAAACAACCGGCGGCGCTGATCCTGC
AGCGATCCACCCGCTAA

Upstream 100 bases:

>100_bases
GAGCGGGTACAATCTTTTTGAGAAAATTCCGCTATTTTTAATTGTTGTCCTTTCACGACGACCCTTCCCTCCATCATAAA
TAAATAGAAAAGGTGTATGC

Downstream 100 bases:

>100_bases
GCGTCGCTGTCGGCCGGTAAGTTAAACCGGTCGACAGCTTACGCACCATTGTTTGCAGAAAAAACCACCGCACTGCACAC
AAGCGCAGCAATCGCGTGAA

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MQKYLIEARSLLALAIPVIIAQISQTAMGVVDTIMAGAYSATDMAAVAVGTSIWLPAILFGHGLLLALTPVIAQLNGAGR
RDRIAHQVRQGFWLAAAVSLLVIIVLYNSKFVIDMMHNIDPQLADKAVGYLHAIMWGAPGYLFFQVLRGQCEGLSKTKPG
MVIGFLGLLVNIPINYIFIYGKFGMPELGGVGCGVATGSVYWFMFLAMRWYVKHAPSQRDILPQEGSSMRPDWAAMKRLF
GIGLPVALALFFEVTLFAVVALLVSPLGIVSVAGHQIALNFSALMFVLPLSLGVSATIRVGYRLGEGSVEGARVAAYSAI
GVGILMACCTALFTATFREPIALLYNDSPAVVAMASQLMLLAAIYQISDSIQVIGSGVLRGYKDTRSIFFITFIAYWILG
LPSGYVLALTDILVPAMGPSGFWIGFIIGLTFAAIMMAMRMRWLQKQPAALILQRSTR

Sequences:

>Translated_458_residues
MQKYLIEARSLLALAIPVIIAQISQTAMGVVDTIMAGAYSATDMAAVAVGTSIWLPAILFGHGLLLALTPVIAQLNGAGR
RDRIAHQVRQGFWLAAAVSLLVIIVLYNSKFVIDMMHNIDPQLADKAVGYLHAIMWGAPGYLFFQVLRGQCEGLSKTKPG
MVIGFLGLLVNIPINYIFIYGKFGMPELGGVGCGVATGSVYWFMFLAMRWYVKHAPSQRDILPQEGSSMRPDWAAMKRLF
GIGLPVALALFFEVTLFAVVALLVSPLGIVSVAGHQIALNFSALMFVLPLSLGVSATIRVGYRLGEGSVEGARVAAYSAI
GVGILMACCTALFTATFREPIALLYNDSPAVVAMASQLMLLAAIYQISDSIQVIGSGVLRGYKDTRSIFFITFIAYWILG
LPSGYVLALTDILVPAMGPSGFWIGFIIGLTFAAIMMAMRMRWLQKQPAALILQRSTR
>Mature_458_residues
MQKYLIEARSLLALAIPVIIAQISQTAMGVVDTIMAGAYSATDMAAVAVGTSIWLPAILFGHGLLLALTPVIAQLNGAGR
RDRIAHQVRQGFWLAAAVSLLVIIVLYNSKFVIDMMHNIDPQLADKAVGYLHAIMWGAPGYLFFQVLRGQCEGLSKTKPG
MVIGFLGLLVNIPINYIFIYGKFGMPELGGVGCGVATGSVYWFMFLAMRWYVKHAPSQRDILPQEGSSMRPDWAAMKRLF
GIGLPVALALFFEVTLFAVVALLVSPLGIVSVAGHQIALNFSALMFVLPLSLGVSATIRVGYRLGEGSVEGARVAAYSAI
GVGILMACCTALFTATFREPIALLYNDSPAVVAMASQLMLLAAIYQISDSIQVIGSGVLRGYKDTRSIFFITFIAYWILG
LPSGYVLALTDILVPAMGPSGFWIGFIIGLTFAAIMMAMRMRWLQKQPAALILQRSTR

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=430, Percent_Identity=20.9302325581395, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI153792564, Length=441, Percent_Identity=19.7278911564626, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI38261960, Length=477, Percent_Identity=19.0775681341719, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI48994890, Length=458, Percent_Identity=75.3275109170306, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=460, Percent_Identity=26.0869565217391, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321821, Length=391, Percent_Identity=21.227621483376, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320545, Length=423, Percent_Identity=19.8581560283688, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49428; Mature: 49428

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA