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The map label for this gene is arnB

Identifier: 157370394

GI number: 157370394

Start: 2350152

End: 2351306

Strand: Direct

Name: arnB

Synonym: Spro_2154

Alternate gene names: 157370394

Gene position: 2350152-2351306 (Clockwise)

Preceding gene: 157370393

Following gene: 157370395

Centisome position: 43.13

GC content: 56.19

Gene sequence:

>1155_bases
ATGGATCAATTTTTACCTTTCTCACGCCCGGCGATTGGCGATGAAGAGATCTCGGCGGTAGAAAAGGTATTGCGCTCAGG
CTGGATCACCACCGGGCCGCAAAATCAGCAGTTGGAAAGTGAGTTCTGTGCCACCTTCGGCTGTAAACACGCCATCGCCG
TCTGTTCCGCTACTGCAGGTATGCATATCACCCTGATGGCGTTGAACATTGGCCCGGGTGACGAAGTTATCACGCCGTCC
CAGACCTGGGTTTCTACCCTCAACATGATTGAATTGCTAGGTGCGACGCCGGTAATGATTGACGTTGACCGCGATACGCT
GATGGTCAGCGCCAGTGATGTTGAAGCCGCCATTACGACGAAAACCAAAGTCATTGTCCCGGTGCACTACGCCGGCGCAC
CGTTGCAGATGGATGCACTGCGTGAGGTGGCAAAACGCCACAATCTCCCCCTGATTGAAGACGCCGCTCATGCGGTGGGC
GCCCGTTACAACGGTGAGTGGGTGGGCGCTCGCGGCACGGCAATTTTCTCATTCCATGCGATTAAAAACCTGACCTGTGC
CGAAGGCGGCCTGATCGCCACCGACGACGACCAACTGGCAGATCGCCTGCGCTGTCTGAAGTTCCACGGCCTGGCCGTTG
ATGCTTTTGATCGCCAGCAGTTGGGGCGTAAACCCCAGGCCGAAGTGGTCGAGCCTGGTTACAAATACAATCTTTCCGAT
ATCCACGCGGCTATCGCCGTGGTGCAGTTGGCTCGCTTGCCGCAGCTTAATGCTCGCCGCAAGGCGCTGGCGCAACGTTA
CCTCACCGCTCTGGAAGGTTCGCCTTTCCTGCCGTTGGGATTACCTGATTATCCGCACGACCATGCCTGGCATCTGTTTA
TGGTGCGCGTTGACGCTGAACGCTGCGGTATCGATCGCGACCAGTTGATGGAACGCCTGAAAGAAATGGGTATCGGTACC
GGCCTGCATTTCCGTGCCGCCCATACCCAGAAGTTTTATCGTGATCGCTATCCGCAGCTTTCCTTGCCCAATACCGAATG
GAACTCGGCACGTCTGTGCACCTTGCCGCTATTCCCTGATATGACCGACGCCGATGTTGATCGCGTGGTTAAAGCCTTGT
CTTCTGTTGTGGAGTCGTTACGTGTCTCGCGTTGA

Upstream 100 bases:

>100_bases
AGTTAATCCATCAGGAACGATTCTCTCTGGCGTTTAATATTCGTTAAATAAAAGAGATCTACAGTGTCATTAAAGCAATT
TGATACGTTTGGGGTTAACT

Downstream 100 bases:

>100_bases
ACCGATAAAAAAAGTTTCGGTGGTGATCCCGGTCTATAACGAGCAGGAAAGTTTGCCTGCCTTGCTCGAACGTACCACCG
CCGCCTGTAAACAACTGACC

Product: UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase

Products: NA

Alternate protein names: UDP-(beta-L-threo-pentapyranosyl-4''-ulose diphosphate) aminotransferase; UDP-Ara4O aminotransferase; UDP-4-amino-4-deoxy-L-arabinose aminotransferase

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MDQFLPFSRPAIGDEEISAVEKVLRSGWITTGPQNQQLESEFCATFGCKHAIAVCSATAGMHITLMALNIGPGDEVITPS
QTWVSTLNMIELLGATPVMIDVDRDTLMVSASDVEAAITTKTKVIVPVHYAGAPLQMDALREVAKRHNLPLIEDAAHAVG
ARYNGEWVGARGTAIFSFHAIKNLTCAEGGLIATDDDQLADRLRCLKFHGLAVDAFDRQQLGRKPQAEVVEPGYKYNLSD
IHAAIAVVQLARLPQLNARRKALAQRYLTALEGSPFLPLGLPDYPHDHAWHLFMVRVDAERCGIDRDQLMERLKEMGIGT
GLHFRAAHTQKFYRDRYPQLSLPNTEWNSARLCTLPLFPDMTDADVDRVVKALSSVVESLRVSR

Sequences:

>Translated_384_residues
MDQFLPFSRPAIGDEEISAVEKVLRSGWITTGPQNQQLESEFCATFGCKHAIAVCSATAGMHITLMALNIGPGDEVITPS
QTWVSTLNMIELLGATPVMIDVDRDTLMVSASDVEAAITTKTKVIVPVHYAGAPLQMDALREVAKRHNLPLIEDAAHAVG
ARYNGEWVGARGTAIFSFHAIKNLTCAEGGLIATDDDQLADRLRCLKFHGLAVDAFDRQQLGRKPQAEVVEPGYKYNLSD
IHAAIAVVQLARLPQLNARRKALAQRYLTALEGSPFLPLGLPDYPHDHAWHLFMVRVDAERCGIDRDQLMERLKEMGIGT
GLHFRAAHTQKFYRDRYPQLSLPNTEWNSARLCTLPLFPDMTDADVDRVVKALSSVVESLRVSR
>Mature_384_residues
MDQFLPFSRPAIGDEEISAVEKVLRSGWITTGPQNQQLESEFCATFGCKHAIAVCSATAGMHITLMALNIGPGDEVITPS
QTWVSTLNMIELLGATPVMIDVDRDTLMVSASDVEAAITTKTKVIVPVHYAGAPLQMDALREVAKRHNLPLIEDAAHAVG
ARYNGEWVGARGTAIFSFHAIKNLTCAEGGLIATDDDQLADRLRCLKFHGLAVDAFDRQQLGRKPQAEVVEPGYKYNLSD
IHAAIAVVQLARLPQLNARRKALAQRYLTALEGSPFLPLGLPDYPHDHAWHLFMVRVDAERCGIDRDQLMERLKEMGIGT
GLHFRAAHTQKFYRDRYPQLSLPNTEWNSARLCTLPLFPDMTDADVDRVVKALSSVVESLRVSR

Specific function: Catalyzes the conversion of UDP-4-keto-arabinose (UDP- Ara4O) to UDP-4-amino-4-deoxy-L-arabinose (UDP-L-Ara4N). The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family. ArnB subfamily

Homologues:

Organism=Escherichia coli, GI145693159, Length=376, Percent_Identity=69.4148936170213, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI2367285, Length=385, Percent_Identity=34.025974025974, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARNB_SERP5 (A8GDR5)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001478383.1
- ProteinModelPortal:   A8GDR5
- SMR:   A8GDR5
- STRING:   A8GDR5
- GeneID:   5605912
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_2154
- eggNOG:   COG0399
- HOGENOM:   HBG660897
- OMA:   NVTCAEG
- ProtClustDB:   PRK11658
- BioCyc:   SPRO399741:SPRO_2154-MONOMER
- HAMAP:   MF_01167
- InterPro:   IPR022850
- InterPro:   IPR000653
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF000390

Pfam domain/function: PF01041 DegT_DnrJ_EryC1; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.6.1.87

Molecular weight: Translated: 42405; Mature: 42405

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQFLPFSRPAIGDEEISAVEKVLRSGWITTGPQNQQLESEFCATFGCKHAIAVCSATAG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCC
MHITLMALNIGPGDEVITPSQTWVSTLNMIELLGATPVMIDVDRDTLMVSASDVEAAITT
CEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEE
KTKVIVPVHYAGAPLQMDALREVAKRHNLPLIEDAAHAVGARYNGEWVGARGTAIFSFHA
CEEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCCCCEEEEEHH
IKNLTCAEGGLIATDDDQLADRLRCLKFHGLAVDAFDRQQLGRKPQAEVVEPGYKYNLSD
HCCCEECCCCEEECCCHHHHHHHHHHHHCCEEEECCCHHHHCCCCCCHHCCCCCCCCHHH
IHAAIAVVQLARLPQLNARRKALAQRYLTALEGSPFLPLGLPDYPHDHAWHLFMVRVDAE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECHH
RCGIDRDQLMERLKEMGIGTGLHFRAAHTQKFYRDRYPQLSLPNTEWNSARLCTLPLFPD
HHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCC
MTDADVDRVVKALSSVVESLRVSR
CCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDQFLPFSRPAIGDEEISAVEKVLRSGWITTGPQNQQLESEFCATFGCKHAIAVCSATAG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCC
MHITLMALNIGPGDEVITPSQTWVSTLNMIELLGATPVMIDVDRDTLMVSASDVEAAITT
CEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCEEEEE
KTKVIVPVHYAGAPLQMDALREVAKRHNLPLIEDAAHAVGARYNGEWVGARGTAIFSFHA
CEEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEECCCCCEEEEEHH
IKNLTCAEGGLIATDDDQLADRLRCLKFHGLAVDAFDRQQLGRKPQAEVVEPGYKYNLSD
HCCCEECCCCEEECCCHHHHHHHHHHHHCCEEEECCCHHHHCCCCCCHHCCCCCCCCHHH
IHAAIAVVQLARLPQLNARRKALAQRYLTALEGSPFLPLGLPDYPHDHAWHLFMVRVDAE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECHH
RCGIDRDQLMERLKEMGIGTGLHFRAAHTQKFYRDRYPQLSLPNTEWNSARLCTLPLFPD
HHCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCC
MTDADVDRVVKALSSVVESLRVSR
CCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA