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The map label for this gene is puuP [H]

Identifier: 157370311

GI number: 157370311

Start: 2261995

End: 2263365

Strand: Direct

Name: puuP [H]

Synonym: Spro_2069

Alternate gene names: 157370311

Gene position: 2261995-2263365 (Clockwise)

Preceding gene: 157370310

Following gene: 157370312

Centisome position: 41.51

GC content: 54.63

Gene sequence:

>1371_bases
ATGACGACTAATGTGACTGACATCAATGAGGCAGCCAAGCCGCAACTGCGTAAAAGCCTGAAGCTGTGGCAAGTGGTGAT
GATGGGCCTGGCCTATCTGACACCAATGACGGTATTCGACACTTTCGGCATCGTCTCCGGCCTTACCGACGGTCACGTAC
CGGCCTCCTATTTACTGGCGCTGGCCGGCGTGCTGTTTACCGCCATCAGCTACGGCAAGCTGGTGCGCCAGTTCCCGACC
GCCGGTTCAGCCTACACCTATGCGCAAAAAGCCATAAATCCGCACGTCGGTTTTTTGGTGGGTTGGTCTTCACTGCTCGA
TTATCTGTTCCTGCCGATGATCAATACCCTGTTGGCGAAGATTTACCTGACGGCGTTGTTCCCGGAAGTGCCGCCGTGGA
TATGGGTGGTGGGCTTTGTGATTATCATTACCGCCATCAACCTGAAAAGCGTCAATCTGGTGGCCAACTTCAATACCCTG
TTCGTACTGGCCCAGGTGGCAATCATTGTGGTGTTTATCTTCCTGGTGGTACGTGGCCTGCACAACGGTGAAGGACTGGG
GACGGTATGGAGCCTGCAACCCTTCGTCAGCGAAAATGCGCATTTACTGCCGATCATCACCGGCGCCACCATCTTGTGTT
TCTCTTTCCTCGGCTTTGATGCGGTGACCACGCTATGTGAAGAAACGCCCGATGCCGCCAGGGTGATCCCGCGCGCTATC
TTTCTGACCGCGCTGTATGGTGGGGTGATCTTCATCAGCGTGTCATTCTTTATCCAACTGTTTTTCCCTTCGATTCAGCG
GTTCCATCAGCCGGATGCGGCACTGCCGGAAATAGCGCTGTACGTGGGCGGCAAGCTGTTCCAGTCGATTTTCCTGTGCG
TCACCTTTATCAACACCCTGGCCTCGGGCCTGGCATCACATGCCAGCGTTTCACGGCTGTTGTATGTGATGGGGCGCGAT
AACGTGTTCCCGGAAAAATATTTTGGCTATATCCACCCGAAATGGCGCACGCCATCACTCAACGTGTTGCTGGTGGGGCT
GGTCGCACTGTCTGCGTTGTCTTTCGATCTGGTCACGGCGACCGCATTGATCAACTTCGGTGCGCTGGTGGCTTTTACCT
TCGTTAATCTGTCGGTGATCAGTCATTTCTATATTCGCGAGGGGCGTAACAAGAGCTGGAAGGATCGCTTCAACTATCTG
TTCCTGCCATTGGTCGGGGCTTTAACTGTCGGCGTGTTGTGGTTAAACCTGGAAAAAAGCTCGCTGACCATGGGACTGAT
CTGGGCGGTTATGGGCTTTGGTTACCTGGCGTATCTCACCCGGCGTTTCCGCCAGCCGCCACCGCAGCTGGAACGACAAC
CGCAGCAATAA

Upstream 100 bases:

>100_bases
TCTTTAAACCGCACTTCCCGCCGCCTGAGCAGCAGTTTCCCCGCGGCCTCTCTGAATGACGTTAAACGGTTACCAGCAGA
GGACAACGAGGGGAAGAGCC

Downstream 100 bases:

>100_bases
CTGAGTCAGGCGGGGTTTCCCGCCTTTCTTTTTTGTCCGTCGGACAGTAGAGTGAAAATACTGATAAAAAATAACCGATT
GGATCACCTCCATGTCTTCA

Product: amino acid permease-associated protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTTNVTDINEAAKPQLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGLTDGHVPASYLLALAGVLFTAISYGKLVRQFPT
AGSAYTYAQKAINPHVGFLVGWSSLLDYLFLPMINTLLAKIYLTALFPEVPPWIWVVGFVIIITAINLKSVNLVANFNTL
FVLAQVAIIVVFIFLVVRGLHNGEGLGTVWSLQPFVSENAHLLPIITGATILCFSFLGFDAVTTLCEETPDAARVIPRAI
FLTALYGGVIFISVSFFIQLFFPSIQRFHQPDAALPEIALYVGGKLFQSIFLCVTFINTLASGLASHASVSRLLYVMGRD
NVFPEKYFGYIHPKWRTPSLNVLLVGLVALSALSFDLVTATALINFGALVAFTFVNLSVISHFYIREGRNKSWKDRFNYL
FLPLVGALTVGVLWLNLEKSSLTMGLIWAVMGFGYLAYLTRRFRQPPPQLERQPQQ

Sequences:

>Translated_456_residues
MTTNVTDINEAAKPQLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGLTDGHVPASYLLALAGVLFTAISYGKLVRQFPT
AGSAYTYAQKAINPHVGFLVGWSSLLDYLFLPMINTLLAKIYLTALFPEVPPWIWVVGFVIIITAINLKSVNLVANFNTL
FVLAQVAIIVVFIFLVVRGLHNGEGLGTVWSLQPFVSENAHLLPIITGATILCFSFLGFDAVTTLCEETPDAARVIPRAI
FLTALYGGVIFISVSFFIQLFFPSIQRFHQPDAALPEIALYVGGKLFQSIFLCVTFINTLASGLASHASVSRLLYVMGRD
NVFPEKYFGYIHPKWRTPSLNVLLVGLVALSALSFDLVTATALINFGALVAFTFVNLSVISHFYIREGRNKSWKDRFNYL
FLPLVGALTVGVLWLNLEKSSLTMGLIWAVMGFGYLAYLTRRFRQPPPQLERQPQQ
>Mature_455_residues
TTNVTDINEAAKPQLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGLTDGHVPASYLLALAGVLFTAISYGKLVRQFPTA
GSAYTYAQKAINPHVGFLVGWSSLLDYLFLPMINTLLAKIYLTALFPEVPPWIWVVGFVIIITAINLKSVNLVANFNTLF
VLAQVAIIVVFIFLVVRGLHNGEGLGTVWSLQPFVSENAHLLPIITGATILCFSFLGFDAVTTLCEETPDAARVIPRAIF
LTALYGGVIFISVSFFIQLFFPSIQRFHQPDAALPEIALYVGGKLFQSIFLCVTFINTLASGLASHASVSRLLYVMGRDN
VFPEKYFGYIHPKWRTPSLNVLLVGLVALSALSFDLVTATALINFGALVAFTFVNLSVISHFYIREGRNKSWKDRFNYLF
LPLVGALTVGVLWLNLEKSSLTMGLIWAVMGFGYLAYLTRRFRQPPPQLERQPQQ

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Homo sapiens, GI4507047, Length=420, Percent_Identity=22.6190476190476, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI114326544, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI114326550, Length=192, Percent_Identity=27.0833333333333, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI181337167, Length=375, Percent_Identity=24, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081869, Length=445, Percent_Identity=83.1460674157303, Blast_Score=759, Evalue=0.0,
Organism=Escherichia coli, GI87082023, Length=451, Percent_Identity=64.7450110864745, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI1788480, Length=401, Percent_Identity=23.9401496259352, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1786789, Length=175, Percent_Identity=31.4285714285714, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786602, Length=270, Percent_Identity=25.5555555555556, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082250, Length=363, Percent_Identity=23.6914600550964, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081708, Length=463, Percent_Identity=22.6781857451404, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786302, Length=173, Percent_Identity=26.0115606936416, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789017, Length=349, Percent_Identity=24.0687679083095, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=441, Percent_Identity=24.7165532879819, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533459, Length=435, Percent_Identity=24.367816091954, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24668806, Length=424, Percent_Identity=25.2358490566038, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356285, Length=424, Percent_Identity=25.2358490566038, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24668802, Length=424, Percent_Identity=25.2358490566038, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24667468, Length=408, Percent_Identity=26.2254901960784, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI221512776, Length=423, Percent_Identity=22.4586288416076, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24666159, Length=423, Percent_Identity=22.4586288416076, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281366235, Length=413, Percent_Identity=24.455205811138, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007820, Length=413, Percent_Identity=24.455205811138, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI116007818, Length=413, Percent_Identity=24.455205811138, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI85725152, Length=413, Percent_Identity=24.455205811138, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI161077963, Length=398, Percent_Identity=23.1155778894472, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 50513; Mature: 50382

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNVTDINEAAKPQLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGLTDGHVPASYLLA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LAGVLFTAISYGKLVRQFPTAGSAYTYAQKAINPHVGFLVGWSSLLDYLFLPMINTLLAK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYLTALFPEVPPWIWVVGFVIIITAINLKSVNLVANFNTLFVLAQVAIIVVFIFLVVRGL
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCC
HNGEGLGTVWSLQPFVSENAHLLPIITGATILCFSFLGFDAVTTLCEETPDAARVIPRAI
CCCCCCCEEEECCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FLTALYGGVIFISVSFFIQLFFPSIQRFHQPDAALPEIALYVGGKLFQSIFLCVTFINTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGLASHASVSRLLYVMGRDNVFPEKYFGYIHPKWRTPSLNVLLVGLVALSALSFDLVTA
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TALINFGALVAFTFVNLSVISHFYIREGRNKSWKDRFNYLFLPLVGALTVGVLWLNLEKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHH
SLTMGLIWAVMGFGYLAYLTRRFRQPPPQLERQPQQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC
>Mature Secondary Structure 
TTNVTDINEAAKPQLRKSLKLWQVVMMGLAYLTPMTVFDTFGIVSGLTDGHVPASYLLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
LAGVLFTAISYGKLVRQFPTAGSAYTYAQKAINPHVGFLVGWSSLLDYLFLPMINTLLAK
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
IYLTALFPEVPPWIWVVGFVIIITAINLKSVNLVANFNTLFVLAQVAIIVVFIFLVVRGL
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHCC
HNGEGLGTVWSLQPFVSENAHLLPIITGATILCFSFLGFDAVTTLCEETPDAARVIPRAI
CCCCCCCEEEECCHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
FLTALYGGVIFISVSFFIQLFFPSIQRFHQPDAALPEIALYVGGKLFQSIFLCVTFINTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ASGLASHASVSRLLYVMGRDNVFPEKYFGYIHPKWRTPSLNVLLVGLVALSALSFDLVTA
HHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TALINFGALVAFTFVNLSVISHFYIREGRNKSWKDRFNYLFLPLVGALTVGVLWLNLEKS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECHH
SLTMGLIWAVMGFGYLAYLTRRFRQPPPQLERQPQQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]