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The map label for this gene is puuA [H]

Identifier: 157370310

GI number: 157370310

Start: 2260259

End: 2261677

Strand: Direct

Name: puuA [H]

Synonym: Spro_2068

Alternate gene names: 157370310

Gene position: 2260259-2261677 (Clockwise)

Preceding gene: 157370305

Following gene: 157370311

Centisome position: 41.48

GC content: 57.65

Gene sequence:

>1419_bases
ATGCAGACCAACATAGTAGAAGTGGAAAACTTTGTTCAGCATCATGAAGAAAAGCGAAGTAGCGCGTTCCAGCGTGAAGT
GAAGAACTACCTGGAGCGTTATCCATTAACTCAGCACGTTGACGTTCTGCTGACCGACCTTAACGGCAGCTTCCGCGGCA
AACGTATTCCGGTCGCTGGGTTGAGCAAACTGGAGAAGGGGTGTTACTTCCCGGCGTCGGTGTTTGCGATGGATATTCTC
GGCAATGTGGTTGAGGAAGCCGGGCTGGGGCAGGAGCTGGGTGAGCCGGATCATATCTGCGTGCCGGTGCTGGGCACCCT
GACACCGTCGGCGGCGGATCCGCAATATATCGGCCAGGTGCTGCTCACCATGCTCGATGAAGATGGTACTCCCTTTGACG
TTGAACCGCGCAATGTGCTGAACCGAGTGTGGCAGCGTTTGCGTCAGCGCGGGCTATCCCCCGTAGTAGCGGTAGAGTTG
GAGTTCTATCTCATCGATCGCCAACGCGATTCGGAAGGCTATCTGCAGCCGCCGTGTGCGCCGGGCACTCAGGAGCGCAA
TACCCAAAGCCAGGTGTATTCGGTTGACAATCTTAACCACTTTGCCGACGTGCTGAGCGAGATTGACGAATTGGCGCGTA
TGCAGGGCCTGCCGGCAGACGGTGCGGTGGCGGAGGCTTCACCCGGCCAGTTTGAAGTCAATCTGCATCATACCGATAAC
GTCCTGCAGGCCTGTGACCATGCGCTGGCATTAAAACGCCTGATCCGCATGGTGGCGGAAAACCACAACATGCAGGCCAC
CTTTATGGCCAAACCCTACGAAGAACACGCCGGTAGCGGCATGCACGTGCATATCAGCATGCTGGACCGGCAGGGCAACA
ACGTACTGGCAGACGACGACGGTGAAGACTCCGCGCTGCTGAAACAAGCGCTGGCCGGGATGATTGCCCTGATGCCTTCT
TCTATTGCGTTGCTGGCACCGAATGTTAACGCCTATCGTCGTTTCCAGCCGGGCATGTACGTGCCGACCCAGGCGTCCTG
GGGCCATAACAACCGCACCGTGGCGCTGCGCATTCCCTGCGGCGATCGCGACAGTCATCGCGTGGAGTATCGCGTAGCCG
GTGCTGATGCCAACCCTTATCTGGTCATGGCGACCATTCTGGCCGGTATTGTCTACGGCCTGGAAACCACCCTGCCGCTG
CAAGAGCCGGTGACTGGCAACGGGCTGGAGCAGGAGGGGCTACCGTTCCCGATCCGCCAAAGCGATGCGCTGTATGAGTT
TGAGCATCAGCCGGTACTGAATGCCCTGCTGGGCGAAAGGTTCAGCCATGTTTACCTGGCTTGTAAAACGGATGAGCTGG
TGCAGTTTGAGCGCCTGATCACCGAAACCGAAATCGAGTGGATGCTGAAAAACGCCTGA

Upstream 100 bases:

>100_bases
ATGTTTCATTGGTGGACATTATATTGAGCAAGATGGTCAAAAAACATCGACGGCAATAAGTGTGAGAACCACAGACAAAA
ACATACATGGCGGGTGAATC

Downstream 100 bases:

>100_bases
CCCGCTCGACGCCAATCCTACCGCGCGCGTATGGCGCATTCCGCCGGCTATCCTGTGGCCTGCGATGCTGCGCGCTGCGG
TAAAGCATTGCTTCCACTTT

Product: glutamate--putrescine ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MQTNIVEVENFVQHHEEKRSSAFQREVKNYLERYPLTQHVDVLLTDLNGSFRGKRIPVAGLSKLEKGCYFPASVFAMDIL
GNVVEEAGLGQELGEPDHICVPVLGTLTPSAADPQYIGQVLLTMLDEDGTPFDVEPRNVLNRVWQRLRQRGLSPVVAVEL
EFYLIDRQRDSEGYLQPPCAPGTQERNTQSQVYSVDNLNHFADVLSEIDELARMQGLPADGAVAEASPGQFEVNLHHTDN
VLQACDHALALKRLIRMVAENHNMQATFMAKPYEEHAGSGMHVHISMLDRQGNNVLADDDGEDSALLKQALAGMIALMPS
SIALLAPNVNAYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRDSHRVEYRVAGADANPYLVMATILAGIVYGLETTLPL
QEPVTGNGLEQEGLPFPIRQSDALYEFEHQPVLNALLGERFSHVYLACKTDELVQFERLITETEIEWMLKNA

Sequences:

>Translated_472_residues
MQTNIVEVENFVQHHEEKRSSAFQREVKNYLERYPLTQHVDVLLTDLNGSFRGKRIPVAGLSKLEKGCYFPASVFAMDIL
GNVVEEAGLGQELGEPDHICVPVLGTLTPSAADPQYIGQVLLTMLDEDGTPFDVEPRNVLNRVWQRLRQRGLSPVVAVEL
EFYLIDRQRDSEGYLQPPCAPGTQERNTQSQVYSVDNLNHFADVLSEIDELARMQGLPADGAVAEASPGQFEVNLHHTDN
VLQACDHALALKRLIRMVAENHNMQATFMAKPYEEHAGSGMHVHISMLDRQGNNVLADDDGEDSALLKQALAGMIALMPS
SIALLAPNVNAYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRDSHRVEYRVAGADANPYLVMATILAGIVYGLETTLPL
QEPVTGNGLEQEGLPFPIRQSDALYEFEHQPVLNALLGERFSHVYLACKTDELVQFERLITETEIEWMLKNA
>Mature_472_residues
MQTNIVEVENFVQHHEEKRSSAFQREVKNYLERYPLTQHVDVLLTDLNGSFRGKRIPVAGLSKLEKGCYFPASVFAMDIL
GNVVEEAGLGQELGEPDHICVPVLGTLTPSAADPQYIGQVLLTMLDEDGTPFDVEPRNVLNRVWQRLRQRGLSPVVAVEL
EFYLIDRQRDSEGYLQPPCAPGTQERNTQSQVYSVDNLNHFADVLSEIDELARMQGLPADGAVAEASPGQFEVNLHHTDN
VLQACDHALALKRLIRMVAENHNMQATFMAKPYEEHAGSGMHVHISMLDRQGNNVLADDDGEDSALLKQALAGMIALMPS
SIALLAPNVNAYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRDSHRVEYRVAGADANPYLVMATILAGIVYGLETTLPL
QEPVTGNGLEQEGLPFPIRQSDALYEFEHQPVLNALLGERFSHVYLACKTDELVQFERLITETEIEWMLKNA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=434, Percent_Identity=25.3456221198157, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI87081870, Length=472, Percent_Identity=81.9915254237288, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1790301, Length=400, Percent_Identity=27, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 52591; Mature: 52591

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNIVEVENFVQHHEEKRSSAFQREVKNYLERYPLTQHVDVLLTDLNGSFRGKRIPVAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCHHH
LSKLEKGCYFPASVFAMDILGNVVEEAGLGQELGEPDHICVPVLGTLTPSAADPQYIGQV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCCCCCCHHHHHHH
LLTMLDEDGTPFDVEPRNVLNRVWQRLRQRGLSPVVAVELEFYLIDRQRDSEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCC
PGTQERNTQSQVYSVDNLNHFADVLSEIDELARMQGLPADGAVAEASPGQFEVNLHHTDN
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHH
VLQACDHALALKRLIRMVAENHNMQATFMAKPYEEHAGSGMHVHISMLDRQGNNVLADDD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCEEEEEEEEECCCCEEECCCC
GEDSALLKQALAGMIALMPSSIALLAPNVNAYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GDRDSHRVEYRVAGADANPYLVMATILAGIVYGLETTLPLQEPVTGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
SDALYEFEHQPVLNALLGERFSHVYLACKTDELVQFERLITETEIEWMLKNA
CCCCEECCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQTNIVEVENFVQHHEEKRSSAFQREVKNYLERYPLTQHVDVLLTDLNGSFRGKRIPVAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCHHH
LSKLEKGCYFPASVFAMDILGNVVEEAGLGQELGEPDHICVPVLGTLTPSAADPQYIGQV
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCCCCCCHHHHHHH
LLTMLDEDGTPFDVEPRNVLNRVWQRLRQRGLSPVVAVELEFYLIDRQRDSEGYLQPPCA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCCCCCCCCCC
PGTQERNTQSQVYSVDNLNHFADVLSEIDELARMQGLPADGAVAEASPGQFEVNLHHTDN
CCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCHH
VLQACDHALALKRLIRMVAENHNMQATFMAKPYEEHAGSGMHVHISMLDRQGNNVLADDD
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCCCEEEEEEEEECCCCEEECCCC
GEDSALLKQALAGMIALMPSSIALLAPNVNAYRRFQPGMYVPTQASWGHNNRTVALRIPC
CCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
GDRDSHRVEYRVAGADANPYLVMATILAGIVYGLETTLPLQEPVTGNGLEQEGLPFPIRQ
CCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
SDALYEFEHQPVLNALLGERFSHVYLACKTDELVQFERLITETEIEWMLKNA
CCCCEECCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]