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The map label for this gene is engD [H]

Identifier: 157370224

GI number: 157370224

Start: 2169368

End: 2170459

Strand: Reverse

Name: engD [H]

Synonym: Spro_1982

Alternate gene names: 157370224

Gene position: 2170459-2169368 (Counterclockwise)

Preceding gene: 157370226

Following gene: 157370223

Centisome position: 39.83

GC content: 56.78

Gene sequence:

>1092_bases
ATGGGATTCAAATGCGGTATCGTCGGCCTGCCTAACGTAGGCAAATCCACCCTGTTCAACGCGTTGACCAAAGCGGGTAT
CGAAGCAGCCAACTTCCCGTTCTGCACCATTGAACCTAATACCGGTGTGGTGCCGATGCCCGATTCGCGTCTGGACCAGT
TGGCCGAGATCGTCAAACCCCAACGCATCCTGCCAACCACCATGGAATTCGTCGACATCGCCGGCCTGGTGAAAGGCGCG
TCCAAAGGCGAAGGCCTGGGCAACCAGTTCCTGACCAACATCCGTGAAACCGAAGCTATCGGCCACGTAGTGCGTTGCTT
CGAGAACGACAACATCATCCACGTGAACAACAAGGTTGACCCGGCTGACGACATCGAAGTGATCAACACCGAGCTGGCAC
TGTCGGATCTGGACACCTGCGAACGCGCTATCCACCGCGTACAGAAGAAAGCCAAAGGCGGCGATAAAGACGCAAAAGCC
GAGCTGGCCGCACTGGAAAAATGCCTGCCACAGCTGGAAAACGCCGGCATGCTGCGTGCACTGGATCTGTCCGATGAAGA
CCGTGCAGCCATCCGCTACCTGAGTTTCCTGACGCTGAAGCCAACCATGTACATCGCCAACGTCAACGAAGACGGCTATG
AGAACAACCCATACCTGGACAAAGTGCGCGAGATCGCTGCGGCCGAAGGTTCGGTCGTGGTTGCCGTGTGCGCCGCGGTT
GAATCCGACATCGCCGAGCTGGAAGACGAAGACCGCGCCGAGTTTATGGCCGAGCTGGGTCTGGAAGAGCCAGGCCTGAA
CCGCGTGATCCGCGCCGGTTACGAGCTGCTGAACCTGCAGACTTACTTCACTGCTGGCGTAAAAGAAGTTCGCGCCTGGA
CCATCCCGGTTGGCGCTACCGCACCGCAGGCAGCCGGCAAGATCCACACCGACTTCGAGAAAGGCTTTATCCGCGCGCAG
ACCATCGCTTTTGAAGACTTCATCACCTACAAGGGTGAGCAAGGCGCTAAAGAAGCTGGCAAAATGCGTTCAGAAGGTAA
GGACTACATCGTCAAAGACGGCGACGTGATGAACTTCTTGTTTAACGTCTAA

Upstream 100 bases:

>100_bases
AAAACGGCCATTACGTGTATAATGGCGCCATTATTTCGTCCTTTATGCAGTGGGTTAAACCTAACCGGCTGATAGTTAAG
ATATTTAAGGTGATAACAAC

Downstream 100 bases:

>100_bases
ACCTCGGTTTCTGCACCAGACTCCAAAATCCACGCCTTGGCGTGGATTTTTGTTTTCTGATTGTTTGAAATCTAGAGCGA
ATATCGGCAAGGCATCTGAC

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDSRLDQLAEIVKPQRILPTTMEFVDIAGLVKGA
SKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVNNKVDPADDIEVINTELALSDLDTCERAIHRVQKKAKGGDKDAKA
ELAALEKCLPQLENAGMLRALDLSDEDRAAIRYLSFLTLKPTMYIANVNEDGYENNPYLDKVREIAAAEGSVVVAVCAAV
ESDIAELEDEDRAEFMAELGLEEPGLNRVIRAGYELLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQ
TIAFEDFITYKGEQGAKEAGKMRSEGKDYIVKDGDVMNFLFNV

Sequences:

>Translated_363_residues
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDSRLDQLAEIVKPQRILPTTMEFVDIAGLVKGA
SKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVNNKVDPADDIEVINTELALSDLDTCERAIHRVQKKAKGGDKDAKA
ELAALEKCLPQLENAGMLRALDLSDEDRAAIRYLSFLTLKPTMYIANVNEDGYENNPYLDKVREIAAAEGSVVVAVCAAV
ESDIAELEDEDRAEFMAELGLEEPGLNRVIRAGYELLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQ
TIAFEDFITYKGEQGAKEAGKMRSEGKDYIVKDGDVMNFLFNV
>Mature_362_residues
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDSRLDQLAEIVKPQRILPTTMEFVDIAGLVKGAS
KGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVNNKVDPADDIEVINTELALSDLDTCERAIHRVQKKAKGGDKDAKAE
LAALEKCLPQLENAGMLRALDLSDEDRAAIRYLSFLTLKPTMYIANVNEDGYENNPYLDKVREIAAAEGSVVVAVCAAVE
SDIAELEDEDRAEFMAELGLEEPGLNRVIRAGYELLNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQT
IAFEDFITYKGEQGAKEAGKMRSEGKDYIVKDGDVMNFLFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=368, Percent_Identity=44.5652173913043, Blast_Score=305, Evalue=6e-83,
Organism=Homo sapiens, GI58761502, Length=227, Percent_Identity=39.2070484581498, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1787454, Length=363, Percent_Identity=93.9393939393939, Blast_Score=703, Evalue=0.0,
Organism=Escherichia coli, GI1789574, Length=127, Percent_Identity=29.9212598425197, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17509631, Length=365, Percent_Identity=40.8219178082192, Blast_Score=284, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6319499, Length=368, Percent_Identity=39.1304347826087, Blast_Score=252, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321773, Length=381, Percent_Identity=34.3832020997375, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321649, Length=142, Percent_Identity=39.4366197183099, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640873, Length=366, Percent_Identity=42.896174863388, Blast_Score=297, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24640877, Length=366, Percent_Identity=42.896174863388, Blast_Score=297, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24640875, Length=366, Percent_Identity=42.896174863388, Blast_Score=297, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24640879, Length=328, Percent_Identity=40.5487804878049, Blast_Score=242, Evalue=2e-64,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 39650; Mature: 39518

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDSRLDQLAEIVKP
CCEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
QRILPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVNNKVD
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PADDIEVINTELALSDLDTCERAIHRVQKKAKGGDKDAKAELAALEKCLPQLENAGMLRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LDLSDEDRAAIRYLSFLTLKPTMYIANVNEDGYENNPYLDKVREIAAAEGSVVVAVCAAV
ECCCCCHHHHHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHH
ESDIAELEDEDRAEFMAELGLEEPGLNRVIRAGYELLNLQTYFTAGVKEVRAWTIPVGAT
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
APQAAGKIHTDFEKGFIRAQTIAFEDFITYKGEQGAKEAGKMRSEGKDYIVKDGDVMNFL
CCHHCCCCCCHHHHCHHHHHHEEHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHHHHH
FNV
HCC
>Mature Secondary Structure 
GFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVVPMPDSRLDQLAEIVKP
CEEECEEECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCEEECCHHHHHHHHHHHCC
QRILPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGHVVRCFENDNIIHVNNKVD
CCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
PADDIEVINTELALSDLDTCERAIHRVQKKAKGGDKDAKAELAALEKCLPQLENAGMLRA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LDLSDEDRAAIRYLSFLTLKPTMYIANVNEDGYENNPYLDKVREIAAAEGSVVVAVCAAV
ECCCCCHHHHHHHHHHHEECCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHHH
ESDIAELEDEDRAEFMAELGLEEPGLNRVIRAGYELLNLQTYFTAGVKEVRAWTIPVGAT
HHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCC
APQAAGKIHTDFEKGFIRAQTIAFEDFITYKGEQGAKEAGKMRSEGKDYIVKDGDVMNFL
CCHHCCCCCCHHHHCHHHHHHEEHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCHHHHHH
FNV
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]