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The map label for this gene is thiK [H]

Identifier: 157370165

GI number: 157370165

Start: 2104550

End: 2105419

Strand: Direct

Name: thiK [H]

Synonym: Spro_1923

Alternate gene names: 157370165

Gene position: 2104550-2105419 (Clockwise)

Preceding gene: 157370164

Following gene: 157370166

Centisome position: 38.62

GC content: 56.78

Gene sequence:

>870_bases
ATGGCGCCGTTACACACTGAAGCCGCACTGCGAAGACTGATTGAGCACGAACTGCCGGCGGTGAAAACCGCCGGTTGTCG
TTTCAGCCCGGTGCAGGGACTGACCGGTGAAAGCTGGCGGATTGAAGGCGAGGGCGTGCAGCTATTGGCTCGCCAGCAAA
GCGCAGAGAAAAGCGCGTTGGGCGTCAGTCGCCGGCGTGAGGCGCGGGTGCTGTGTCGTGTCGGTAAGGACATTGGGCCG
CAGGTTTTAGCGCAGAATAATCAGTGGATTATCTTGCAATGGCTCACTGGTGACGTTGTCACTGAGGCGAATTTTACCCG
CTTGAATGAGGGCGGCAATCTGGCTGAGTTGATGGCCGGTTTGCATCAACGGTCGCCCAGTGGTTACCCGCTGAGCCTGC
ATCGGCAGTTTACTCGTTACTGGCAGCAGCTGGATCCCCGACGCCTGACACCCGCCTGGCTAAAGTTACATCAGCGTTTT
CTGCGTCGTCGGCCCCCAACGCCGCTAAAACTGGCTCCCCTGCATATGGATATTCATCCGGGCAATCTGATTGCACAGGG
CCAGGGTTTGCGGCTGATTGATTGGGAGTATTCGGCCGACGGGGATATCGCGCTGGACATCGCCGCACTGTTTCGCGGCA
ATGCCTGGTCGCCAGCCGAACAACTGCGATTTTTACGGCATTATGCTCGCATCGGCTACTCAAATTTACCCTTGCTGAGC
GCACAGGTGCAGCGTTGGTTGCCGTGGGTAGATTATCTGATGCTGTTATGGTTTGAAGTGCGCTGGCAACAAACCGGCGA
TCCCGAATTTTTGCGCTGGGGCGCAGTGTTGCACCGGCGTTTTTGTTTAACATCCCCATGTTCTGAATAA

Upstream 100 bases:

>100_bases
CAGTATGTGCTGTACAGCGACGTCAGTGGTGATGTGAAGTCACCTACGCTGGATATGCAATTGATGCTGGTGCAAAGCGG
TGAAATCGTCTGGTCAGGCA

Downstream 100 bases:

>100_bases
ATAGTGAAGTGAGGTGGCCGTGGGCCCAGTAATGTTAGATGTCGCCAGCTATGAGCTGGACGCGGAAGAACGTGAGATCC
TGCAACACCCGATGGTGGGC

Product: thiamine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MAPLHTEAALRRLIEHELPAVKTAGCRFSPVQGLTGESWRIEGEGVQLLARQQSAEKSALGVSRRREARVLCRVGKDIGP
QVLAQNNQWIILQWLTGDVVTEANFTRLNEGGNLAELMAGLHQRSPSGYPLSLHRQFTRYWQQLDPRRLTPAWLKLHQRF
LRRRPPTPLKLAPLHMDIHPGNLIAQGQGLRLIDWEYSADGDIALDIAALFRGNAWSPAEQLRFLRHYARIGYSNLPLLS
AQVQRWLPWVDYLMLLWFEVRWQQTGDPEFLRWGAVLHRRFCLTSPCSE

Sequences:

>Translated_289_residues
MAPLHTEAALRRLIEHELPAVKTAGCRFSPVQGLTGESWRIEGEGVQLLARQQSAEKSALGVSRRREARVLCRVGKDIGP
QVLAQNNQWIILQWLTGDVVTEANFTRLNEGGNLAELMAGLHQRSPSGYPLSLHRQFTRYWQQLDPRRLTPAWLKLHQRF
LRRRPPTPLKLAPLHMDIHPGNLIAQGQGLRLIDWEYSADGDIALDIAALFRGNAWSPAEQLRFLRHYARIGYSNLPLLS
AQVQRWLPWVDYLMLLWFEVRWQQTGDPEFLRWGAVLHRRFCLTSPCSE
>Mature_288_residues
APLHTEAALRRLIEHELPAVKTAGCRFSPVQGLTGESWRIEGEGVQLLARQQSAEKSALGVSRRREARVLCRVGKDIGPQ
VLAQNNQWIILQWLTGDVVTEANFTRLNEGGNLAELMAGLHQRSPSGYPLSLHRQFTRYWQQLDPRRLTPAWLKLHQRFL
RRRPPTPLKLAPLHMDIHPGNLIAQGQGLRLIDWEYSADGDIALDIAALFRGNAWSPAEQLRFLRHYARIGYSNLPLLSA
QVQRWLPWVDYLMLLWFEVRWQQTGDPEFLRWGAVLHRRFCLTSPCSE

Specific function: Catalyzes the phosphorylation of thiamine to thiamine phosphate [H]

COG id: COG0510

COG function: function code M; Predicted choline kinase involved in LPS biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787349, Length=240, Percent_Identity=38.3333333333333, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009
- InterPro:   IPR014093 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.89 [H]

Molecular weight: Translated: 33201; Mature: 33069

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPLHTEAALRRLIEHELPAVKTAGCRFSPVQGLTGESWRIEGEGVQLLARQQSAEKSAL
CCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
GVSRRREARVLCRVGKDIGPQVLAQNNQWIILQWLTGDVVTEANFTRLNEGGNLAELMAG
HHHHHHHHHHHHHHCCCCCCHHEECCCCEEEEEEECCCEEECCCCEEECCCCCHHHHHHH
LHQRSPSGYPLSLHRQFTRYWQQLDPRRLTPAWLKLHQRFLRRRPPTPLKLAPLHMDIHP
HHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
GNLIAQGQGLRLIDWEYSADGDIALDIAALFRGNAWSPAEQLRFLRHYARIGYSNLPLLS
CCEEECCCCCEEEEECCCCCCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
AQVQRWLPWVDYLMLLWFEVRWQQTGDPEFLRWGAVLHRRFCLTSPCSE
HHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
APLHTEAALRRLIEHELPAVKTAGCRFSPVQGLTGESWRIEGEGVQLLARQQSAEKSAL
CCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
GVSRRREARVLCRVGKDIGPQVLAQNNQWIILQWLTGDVVTEANFTRLNEGGNLAELMAG
HHHHHHHHHHHHHHCCCCCCHHEECCCCEEEEEEECCCEEECCCCEEECCCCCHHHHHHH
LHQRSPSGYPLSLHRQFTRYWQQLDPRRLTPAWLKLHQRFLRRRPPTPLKLAPLHMDIHP
HHHCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEEEECC
GNLIAQGQGLRLIDWEYSADGDIALDIAALFRGNAWSPAEQLRFLRHYARIGYSNLPLLS
CCEEECCCCCEEEEECCCCCCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHH
AQVQRWLPWVDYLMLLWFEVRWQQTGDPEFLRWGAVLHRRFCLTSPCSE
HHHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA