BacMap

Definition	Serratia proteamaculans 568 chromosome, complete genome.
Accession	NC_009832
Length	5,448,853

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The map label for this gene is caiD [H]

Identifier: 157370070

GI number: 157370070

Start: 2010981

End: 2011703

Strand: Reverse

Name: caiD [H]

Synonym: Spro_1828

Alternate gene names: 157370070

Gene position: 2011703-2010981 (Counterclockwise)

Preceding gene: 157370071

Following gene: 157370068

Centisome position: 36.92

GC content: 60.58

Gene sequence:

>723_bases
ATGAGTGATCTGCCCCTCCCGGTGCGGTATGAGCAAAATGGCGGCATTGCCGTGATTACCCTGAACCGGCCGGAAAAAAA
GAACGCCATCAATCTGGCGATGGCGCAACTGATCCAGGGCTATCTGCAACGGGCCGAACAGGATGATGCCGTTCGGGTGA
TAGTGCTGACCGGCCTGCCGCAAGTGTTTTCCGCCGGAATGGACGTCCGGGCCTTCCAGCAGGGAGAACTGCCGGTGGTC
GAACCGGAAGGATTTGGCGGTCTGGTGCATGCCCAGCTCACCAAGGTGATTATTGCCGCAGTAGATGGCATCGCCTTCGG
CGGCGGTTTCGAGATAGCGCTGGCCTGCGACCTGATCGTCGCCAGCCACAGCGCACAGTTCAGTTTCCCGGAAACCGGGC
TGGGATTGATTGCCGCACAGGGGGGCTGTTCACGGCTGCCGGCGCGCATCTCACCCTATATCGCTCTCGAGTGGTTACTG
ACCGGCCGAATCATCAGCGCGCAGGAAGCCTGGCAACAGGGTGCCATTTCCCGCATCAGTGAGGGTAGCGCACACGAGGA
GGCACTGACACTCGCCCGGCAGATTGCGCAAAAAAATCCGCTGGCGAGTCAGGCGGTGAAGGCCATCGTCCGCCAAGGTT
TGAACCGGCATGAAGCCGCTTCCTTTGACTATCAGCAAGGCTGGGTGGAGCGGGTGCGCCAGGCCGCGGCCAAATCCTTT
TAA

Upstream 100 bases:

>100_bases
CTGTATGCGCTGCCGGCATTTTATCTCCCCTTGTTCTCGCTGCCGCTGGGGGCTATCGCCCTGCTGCTGACGCTGTTTTT
CTTCCAGGGGGAAAAAACCA

Downstream 100 bases:

>100_bases
GAACATTCTTAGTGAATGGCAATCATTCGCAGTCTAATTTTTAATCACTACAAGACAGTGCTTGAGAAGCCATTAACCCC
GCTCTGCGGGGCTTTTTTTG

Product: enoyl-CoA hydratase/isomerase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MSDLPLPVRYEQNGGIAVITLNRPEKKNAINLAMAQLIQGYLQRAEQDDAVRVIVLTGLPQVFSAGMDVRAFQQGELPVV
EPEGFGGLVHAQLTKVIIAAVDGIAFGGGFEIALACDLIVASHSAQFSFPETGLGLIAAQGGCSRLPARISPYIALEWLL
TGRIISAQEAWQQGAISRISEGSAHEEALTLARQIAQKNPLASQAVKAIVRQGLNRHEAASFDYQQGWVERVRQAAAKSF

Sequences:

>Translated_240_residues
MSDLPLPVRYEQNGGIAVITLNRPEKKNAINLAMAQLIQGYLQRAEQDDAVRVIVLTGLPQVFSAGMDVRAFQQGELPVV
EPEGFGGLVHAQLTKVIIAAVDGIAFGGGFEIALACDLIVASHSAQFSFPETGLGLIAAQGGCSRLPARISPYIALEWLL
TGRIISAQEAWQQGAISRISEGSAHEEALTLARQIAQKNPLASQAVKAIVRQGLNRHEAASFDYQQGWVERVRQAAAKSF
>Mature_239_residues
SDLPLPVRYEQNGGIAVITLNRPEKKNAINLAMAQLIQGYLQRAEQDDAVRVIVLTGLPQVFSAGMDVRAFQQGELPVVE
PEGFGGLVHAQLTKVIIAAVDGIAFGGGFEIALACDLIVASHSAQFSFPETGLGLIAAQGGCSRLPARISPYIALEWLLT
GRIISAQEAWQQGAISRISEGSAHEEALTLARQIAQKNPLASQAVKAIVRQGLNRHEAASFDYQQGWVERVRQAAAKSF

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=209, Percent_Identity=37.799043062201, Blast_Score=120, Evalue=8e-28,
Organism=Homo sapiens, GI31542718, Length=199, Percent_Identity=32.6633165829146, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI68989263, Length=198, Percent_Identity=33.8383838383838, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI4502327, Length=223, Percent_Identity=32.7354260089686, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI45643119, Length=209, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260275230, Length=209, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI260274832, Length=209, Percent_Identity=31.5789473684211, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI70995211, Length=233, Percent_Identity=28.3261802575107, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI221136756, Length=216, Percent_Identity=28.2407407407407, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221136753, Length=216, Percent_Identity=28.2407407407407, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI221307494, Length=215, Percent_Identity=28.3720930232558, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4757966, Length=212, Percent_Identity=27.8301886792453, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI51538581, Length=212, Percent_Identity=27.8301886792453, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI25453481, Length=212, Percent_Identity=27.8301886792453, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI51538573, Length=212, Percent_Identity=27.8301886792453, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI4757968, Length=212, Percent_Identity=26.8867924528302, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=212, Percent_Identity=26.8867924528302, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI37594469, Length=213, Percent_Identity=30.9859154929577, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI37594471, Length=213, Percent_Identity=30.9859154929577, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI20127408, Length=191, Percent_Identity=26.7015706806283, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI157694516, Length=216, Percent_Identity=27.3148148148148, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI213417737, Length=216, Percent_Identity=27.3148148148148, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI221142681, Length=205, Percent_Identity=41.4634146341463, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787659, Length=202, Percent_Identity=36.1386138613861, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1788682, Length=213, Percent_Identity=30.5164319248826, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082183, Length=237, Percent_Identity=29.535864978903, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787660, Length=226, Percent_Identity=30.9734513274336, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788597, Length=215, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790281, Length=210, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=196, Percent_Identity=37.7551020408163, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560910, Length=227, Percent_Identity=35.6828193832599, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=217, Percent_Identity=33.1797235023041, Blast_Score=110, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=209, Percent_Identity=30.1435406698565, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=230, Percent_Identity=28.695652173913, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=180, Percent_Identity=32.2222222222222, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=31.3186813186813, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=239, Percent_Identity=27.1966527196653, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17508953, Length=207, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=207, Percent_Identity=30.4347826086957, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=194, Percent_Identity=27.8350515463918, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=26.8041237113402, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24653139, Length=223, Percent_Identity=34.0807174887892, Blast_Score=120, Evalue=7e-28,
Organism=Drosophila melanogaster, GI20129971, Length=199, Percent_Identity=35.1758793969849, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24653477, Length=199, Percent_Identity=35.1758793969849, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357171, Length=200, Percent_Identity=33, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24650670, Length=202, Percent_Identity=34.6534653465347, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24654903, Length=185, Percent_Identity=31.3513513513514, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28571729, Length=172, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571730, Length=172, Percent_Identity=32.5581395348837, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922422, Length=185, Percent_Identity=26.4864864864865, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920382, Length=225, Percent_Identity=27.1111111111111, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 25754; Mature: 25623

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLPLPVRYEQNGGIAVITLNRPEKKNAINLAMAQLIQGYLQRAEQDDAVRVIVLTGLP
CCCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
QVFSAGMDVRAFQQGELPVVEPEGFGGLVHAQLTKVIIAAVDGIAFGGGFEIALACDLIV
HHHHCCCCEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCEEECCCEEEHHHHHHHH
ASHSAQFSFPETGLGLIAAQGGCSRLPARISPYIALEWLLTGRIISAQEAWQQGAISRIS
HCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
EGSAHEEALTLARQIAQKNPLASQAVKAIVRQGLNRHEAASFDYQQGWVERVRQAAAKSF
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDLPLPVRYEQNGGIAVITLNRPEKKNAINLAMAQLIQGYLQRAEQDDAVRVIVLTGLP
CCCCCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
QVFSAGMDVRAFQQGELPVVEPEGFGGLVHAQLTKVIIAAVDGIAFGGGFEIALACDLIV
HHHHCCCCEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCEEECCCEEEHHHHHHHH
ASHSAQFSFPETGLGLIAAQGGCSRLPARISPYIALEWLLTGRIISAQEAWQQGAISRIS
HCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
EGSAHEEALTLARQIAQKNPLASQAVKAIVRQGLNRHEAASFDYQQGWVERVRQAAAKSF
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA