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The map label for this gene is rlmI

Identifier: 157370007

GI number: 157370007

Start: 1943277

End: 1944467

Strand: Reverse

Name: rlmI

Synonym: Spro_1764

Alternate gene names: 157370007

Gene position: 1944467-1943277 (Counterclockwise)

Preceding gene: 157370009

Following gene: 157370006

Centisome position: 35.69

GC content: 54.49

Gene sequence:

>1191_bases
ATGACCGTACGCCTGTTTCTCGCCAAAGGACGTGAAAAGTCCTTACTCCGTCGCCACCCATGGGTGTTTTCCGGCGCTGT
TCAACGTGTCGAAGGTAAAGCCCTGTCTGGCGAAACCATCGATATTCACGACGCCCAGGGCAAATGGCTTGCACGCGGTG
CCTATTCACCGGAGTCGCAAATCCGCGCCCGCGTCTGGACCTTTTTGCCGGACGAAGAGATTAACATCGAATTCTTCATC
CGCCGTTTACAACAGGCGCAAAACTGGCGTGATTGGGTTGCCAAACGTGATGGGCTGGACGGTTACCGTCTGATCGCCGG
CGAATCCGACGGTATGCCGGGCATTACTATCGATCGCTTCCAGAACTTCCTGGTGTTGCAGTTGCTGTCCGCCGGCGCTG
AATATCAGCGTGCCGCGTTGATTACCGCTTTGCAGCATTGTTACCCGGAATGTGCCATCTACGATCGTTCAGACGTCGCG
GTACGTAAAAAAGAAGGTTTACCCTTGGCTCAGGGTCAGGTCGTGGGCGATCTGCCCCCTGCTCTGCTGCCTATCACCGA
ACATGGCATGAAGCTGCTGGTGGATATTCAGCAGGGCCATAAGACCGGTTTTTACCTCGACCAGCGCGACAGCCGTCTGG
CCGCACGTAACTATTCCGCCGGCCGCCGCGTGCTTAACTGCTTCTCCTATACCGGTGCCTTCGCCGTGTCGGCCCTGATG
GGCGGTTGTACTCAGGTGATCAGCGTTGATACCTCGCAAGCCGCATTGGATATCGCCAAACAGAACGTCGAGCTAAACCA
GTTAGACCTGAGCAAAGCAGAATTTGTCCGTGACGACGTGTTCCAACTGCTGCGCGCTTACCGCACGCAGGGTGAAAAAT
TCGACCTGATCATTATGGATCCGCCGAAGTTCGTCGAAAACAAGAATCAACTGGCCGGTGCTTGCCGCGGTTACAAAGAT
ATCAATATGTTGGCGCTGCAGCTGCTGAACCCAGGCGGCATTTTGCTCAGCTTCTCCTGTTCCGGTCTGATGCCAACCGA
TTTATTCCAGAAAATTTTGGCCGATGCCGCTGTAGATGCCGGGCGTGATGTACAATTTATAGAGCAGTATCGTCAGGCTG
CCGATCACCCGGTTATCGCGACTTATCCTGAAGGGCTGTATTTAAAAGGGTTTGCGTGTCGGGTGATGTAA

Upstream 100 bases:

>100_bases
TGTGTCATAATCGGCCCCTTATCGACTTTCGTATATGGTATTGCCTGTGCATCTGCACGGCAACGCCACTCATTTTATCT
AGCTGGCAGCATTCTTAATT

Downstream 100 bases:

>100_bases
CTTGAAATACGCTGTTTTGCCCTCATATAGAGAGCAAGGCATTGTTTCTCAGGAGGTCATCATGATTGCCAGCAAATTCG
GTATCGGACAACAGGTTCGG

Product: PUA domain-containing protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTVRLFLAKGREKSLLRRHPWVFSGAVQRVEGKALSGETIDIHDAQGKWLARGAYSPESQIRARVWTFLPDEEINIEFFI
RRLQQAQNWRDWVAKRDGLDGYRLIAGESDGMPGITIDRFQNFLVLQLLSAGAEYQRAALITALQHCYPECAIYDRSDVA
VRKKEGLPLAQGQVVGDLPPALLPITEHGMKLLVDIQQGHKTGFYLDQRDSRLAARNYSAGRRVLNCFSYTGAFAVSALM
GGCTQVISVDTSQAALDIAKQNVELNQLDLSKAEFVRDDVFQLLRAYRTQGEKFDLIIMDPPKFVENKNQLAGACRGYKD
INMLALQLLNPGGILLSFSCSGLMPTDLFQKILADAAVDAGRDVQFIEQYRQAADHPVIATYPEGLYLKGFACRVM

Sequences:

>Translated_396_residues
MTVRLFLAKGREKSLLRRHPWVFSGAVQRVEGKALSGETIDIHDAQGKWLARGAYSPESQIRARVWTFLPDEEINIEFFI
RRLQQAQNWRDWVAKRDGLDGYRLIAGESDGMPGITIDRFQNFLVLQLLSAGAEYQRAALITALQHCYPECAIYDRSDVA
VRKKEGLPLAQGQVVGDLPPALLPITEHGMKLLVDIQQGHKTGFYLDQRDSRLAARNYSAGRRVLNCFSYTGAFAVSALM
GGCTQVISVDTSQAALDIAKQNVELNQLDLSKAEFVRDDVFQLLRAYRTQGEKFDLIIMDPPKFVENKNQLAGACRGYKD
INMLALQLLNPGGILLSFSCSGLMPTDLFQKILADAAVDAGRDVQFIEQYRQAADHPVIATYPEGLYLKGFACRVM
>Mature_395_residues
TVRLFLAKGREKSLLRRHPWVFSGAVQRVEGKALSGETIDIHDAQGKWLARGAYSPESQIRARVWTFLPDEEINIEFFIR
RLQQAQNWRDWVAKRDGLDGYRLIAGESDGMPGITIDRFQNFLVLQLLSAGAEYQRAALITALQHCYPECAIYDRSDVAV
RKKEGLPLAQGQVVGDLPPALLPITEHGMKLLVDIQQGHKTGFYLDQRDSRLAARNYSAGRRVLNCFSYTGAFAVSALMG
GCTQVISVDTSQAALDIAKQNVELNQLDLSKAEFVRDDVFQLLRAYRTQGEKFDLIIMDPPKFVENKNQLAGACRGYKDI
NMLALQLLNPGGILLSFSCSGLMPTDLFQKILADAAVDAGRDVQFIEQYRQAADHPVIATYPEGLYLKGFACRVM

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Escherichia coli, GI145693111, Length=396, Percent_Identity=84.0909090909091, Blast_Score=699, Evalue=0.0,
Organism=Escherichia coli, GI1787181, Length=234, Percent_Identity=28.2051282051282, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMI_SERP5 (A8GCM8)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477996.1
- ProteinModelPortal:   A8GCM8
- SMR:   A8GCM8
- STRING:   A8GCM8
- GeneID:   5604935
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1764
- eggNOG:   COG1092
- HOGENOM:   HBG411897
- OMA:   RAAYSPH
- ProtClustDB:   PRK15128
- BioCyc:   SPRO399741:SPRO_1764-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01857
- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614
- SMART:   SM00359

Pfam domain/function: PF10672 Methyltrans_SAM; SSF88697 PUA-like

EC number: =2.1.1.191

Molecular weight: Translated: 44264; Mature: 44133

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRLFLAKGREKSLLRRHPWVFSGAVQRVEGKALSGETIDIHDAQGKWLARGAYSPESQ
CEEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCHHH
IRARVWTFLPDEEINIEFFIRRLQQAQNWRDWVAKRDGLDGYRLIAGESDGMPGITIDRF
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHH
QNFLVLQLLSAGAEYQRAALITALQHCYPECAIYDRSDVAVRKKEGLPLAQGQVVGDLPP
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCEECCCCC
ALLPITEHGMKLLVDIQQGHKTGFYLDQRDSRLAARNYSAGRRVLNCFSYTGAFAVSALM
CEECHHHCCEEEEEEECCCCCCCCEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GGCTQVISVDTSQAALDIAKQNVELNQLDLSKAEFVRDDVFQLLRAYRTQGEKFDLIIMD
HCCHHEEECCCHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKNQLAGACRGYKDINMLALQLLNPGGILLSFSCSGLMPTDLFQKILADAAVDA
CHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
GRDVQFIEQYRQAADHPVIATYPEGLYLKGFACRVM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC
>Mature Secondary Structure 
TVRLFLAKGREKSLLRRHPWVFSGAVQRVEGKALSGETIDIHDAQGKWLARGAYSPESQ
EEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCHHH
IRARVWTFLPDEEINIEFFIRRLQQAQNWRDWVAKRDGLDGYRLIAGESDGMPGITIDRF
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEHHHH
QNFLVLQLLSAGAEYQRAALITALQHCYPECAIYDRSDVAVRKKEGLPLAQGQVVGDLPP
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCEECCCCC
ALLPITEHGMKLLVDIQQGHKTGFYLDQRDSRLAARNYSAGRRVLNCFSYTGAFAVSALM
CEECHHHCCEEEEEEECCCCCCCCEECCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
GGCTQVISVDTSQAALDIAKQNVELNQLDLSKAEFVRDDVFQLLRAYRTQGEKFDLIIMD
HCCHHEEECCCHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
PPKFVENKNQLAGACRGYKDINMLALQLLNPGGILLSFSCSGLMPTDLFQKILADAAVDA
CHHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
GRDVQFIEQYRQAADHPVIATYPEGLYLKGFACRVM
CCCHHHHHHHHHHCCCCEEEECCCCEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA