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The map label for this gene is helD [H]

Identifier: 157370002

GI number: 157370002

Start: 1938823

End: 1940877

Strand: Direct

Name: helD [H]

Synonym: Spro_1759

Alternate gene names: 157370002

Gene position: 1938823-1940877 (Clockwise)

Preceding gene: 157369999

Following gene: 157370005

Centisome position: 35.58

GC content: 54.6

Gene sequence:

>2055_bases
ATGGAACTTAAAGCGACATCGCTGGGGAAACACCTGGCTCAGCATCCTTACAACCGTGTGCGGTTGCTGCACGCCGGTGT
CGAGGTGAGCGGTGAGAAGCATGAATATCTTATTCCTTTCAATCAGTTGATTCATGTCCGGTGTAAGCGGGGCATTGTCT
GGGGCGAGCTGGAGTTTGAGCTGCCGGAGGAGAAAGTGGTGCGCCTGCATGGTACCGAATGGCAGGAAACCCAGCGCTTC
TATCACTATCTCCAGCAGGCCTGGCAACAGTGGAGCGCCGAAATGAGCGAAGTTAGCGCCGGTGTGTTGCAGCAACAGGT
TGAACATATCCAGCGTATTGAACAGCAGGATAAGTGGTTTAAAAAATCCGAACTGGGCAAGCTGCAGCAACAAATTCGCG
AGTCTTTCGGTGCCTTACCTATGCCGGTGGCGCGACTGGAGCAATTTGACAACTGTCGGGATGATTATCTCCTTTGTCAG
CAATGGCTGCAGCATGGGTTGCAAAGTGTCGGGCAGCGTAATCAGCAGTGGACCGAACGAATGCTGGAGAGCTACCACGA
CTTCTTCCAGAACATCGAAACTTCCCCCCTGAACGACTCACAAAGCCGCGCAGTAGTTAATGGCGAAGATTCAGTGTTGG
TCCTGGCCGGGGCCGGCAGTGGGAAAACCTCGGTGTTGGTGGCGCGAGCCGGTTGGCTGCTGCGTCGCCAGGAGGCTGAA
CCGGGGCAAATACTGTTGTTGGCGTTTGGCCGTCAGGCGGCGGAAGAGATGAACGAGCGCATTCAGGAGCGCCTGGGCAG
CGTGGCGATCCAGGCCAAAACCTTCCACGCATTGGCACTGCAGATTATTCAGCAGGGCAACCGCAAGGTACCGGCGATCA
GTAAGCTGGAAACCGACAGTAAGGCTCGAAGAGCTTTGTTGATAACCCATTGGCAGCAACAATGTGCAGAAAAAAAAGCA
CAGGCTAAAGGGTGGCGCCAGTGGTTGCTCGAAGAACTGGAATGGGAAGTGCCGGAAGGTGATTTTTGGCAGGATAAACG
CCTGGCAGAACGGCTGGCTGGACGACTGGAACGTTGGCTGGGGTTGATGCGAATGCACGGCGGTAGCCAGGCGGAAATGA
TCGAACAGGCAGATGAAGAGGTCCGTGACCTGTTTTCAAAACGTATCCGGCTGATGGCACCGTTGCTCAAGGCATGGAAA
GGCGCACTGAAAGATGAGGGGGCAGTAGACTTCTCTGGCCTGATCCATCAGGCGGTCAATATTCTGGAGAAAGGGCGTTT
TGTCAGCCCGTGGAAACATATTCTGGTTGATGAGTTTCAGGATATCTCACCGCAACGAGCGCAACTGCTGGCGGCGTTAC
GTAAACAAAACAAACAGACTTGCCTGTTTACCGTGGGTGATGACTGGCAGGCCATTTACCGTTTCAGCGGGGCTGAACTG
ACGCTGACCACAGCATTTGCCGAGAACTTTGGCGAAGGGGCACAATGTGCGCTCGACACCACTTACCGTTTCAATGAACG
CATTGGTGAGGTGGCAAACCGCTTTGTACAGCAGAACCCGCACCAGTTGAAGAAACCGCTGAACAGCCTGAGCAAAGGCA
ACAAGAAATCGGTGGTTCTGTTGCCGCATGAACAACTCGAAAATTTGTTGGATAAGCTCAGTGGCTATGCCAAGCCCGAA
GAGCGGATTCTGGTGTTGGCACGTTACCATCATCTGAAGCCGCAGGCGTTGTCAAAAGCGGCAACCCGCTGGCCTAAACT
GACCATCGAGTTTATGACCATCCACGCCAGCAAAGGGCAGCAGGCCGAGTACGTGATTATCGCCGGTTTGCACCAGGGAA
ACGACGGTTTTCCTGCGGTCGCGCGTGAGTCGATATTAGAAGACGTTCTGTTGCCGCAGCCGGAAGACTTCCCGGATGCC
GAGGAGCGGCGACTGCTGTATGTGGCGTTAACTCGCGCCAAGCATCAGGTGTGGTTATTGCAGGATCGCGAGCGCCCGTC
GGTGTTCGTTGAACAACTGCAGGATCTCGGCGTACCCTCGCAGAAGAAACCCTAA

Upstream 100 bases:

>100_bases
TCTATTCTACCCTTTTTTTAGCGCTAGAGTGCCACTGCTTATCGCAGGTAAACTGGCAGATATCCGTTATTTAATCTCAA
CATAGTCATGGCGCGACGAT

Downstream 100 bases:

>100_bases
ACCCAATGCCTGGCGGAAACCAGGCCGTGATGGTTACTTCAGACGGTCCTGCAAGTAGCGTTGGTAGTCAGGGATGGCGA
TTTCTACTTCGCTTTTGAAC

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MELKATSLGKHLAQHPYNRVRLLHAGVEVSGEKHEYLIPFNQLIHVRCKRGIVWGELEFELPEEKVVRLHGTEWQETQRF
YHYLQQAWQQWSAEMSEVSAGVLQQQVEHIQRIEQQDKWFKKSELGKLQQQIRESFGALPMPVARLEQFDNCRDDYLLCQ
QWLQHGLQSVGQRNQQWTERMLESYHDFFQNIETSPLNDSQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRQEAE
PGQILLLAFGRQAAEEMNERIQERLGSVAIQAKTFHALALQIIQQGNRKVPAISKLETDSKARRALLITHWQQQCAEKKA
QAKGWRQWLLEELEWEVPEGDFWQDKRLAERLAGRLERWLGLMRMHGGSQAEMIEQADEEVRDLFSKRIRLMAPLLKAWK
GALKDEGAVDFSGLIHQAVNILEKGRFVSPWKHILVDEFQDISPQRAQLLAALRKQNKQTCLFTVGDDWQAIYRFSGAEL
TLTTAFAENFGEGAQCALDTTYRFNERIGEVANRFVQQNPHQLKKPLNSLSKGNKKSVVLLPHEQLENLLDKLSGYAKPE
ERILVLARYHHLKPQALSKAATRWPKLTIEFMTIHASKGQQAEYVIIAGLHQGNDGFPAVARESILEDVLLPQPEDFPDA
EERRLLYVALTRAKHQVWLLQDRERPSVFVEQLQDLGVPSQKKP

Sequences:

>Translated_684_residues
MELKATSLGKHLAQHPYNRVRLLHAGVEVSGEKHEYLIPFNQLIHVRCKRGIVWGELEFELPEEKVVRLHGTEWQETQRF
YHYLQQAWQQWSAEMSEVSAGVLQQQVEHIQRIEQQDKWFKKSELGKLQQQIRESFGALPMPVARLEQFDNCRDDYLLCQ
QWLQHGLQSVGQRNQQWTERMLESYHDFFQNIETSPLNDSQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRQEAE
PGQILLLAFGRQAAEEMNERIQERLGSVAIQAKTFHALALQIIQQGNRKVPAISKLETDSKARRALLITHWQQQCAEKKA
QAKGWRQWLLEELEWEVPEGDFWQDKRLAERLAGRLERWLGLMRMHGGSQAEMIEQADEEVRDLFSKRIRLMAPLLKAWK
GALKDEGAVDFSGLIHQAVNILEKGRFVSPWKHILVDEFQDISPQRAQLLAALRKQNKQTCLFTVGDDWQAIYRFSGAEL
TLTTAFAENFGEGAQCALDTTYRFNERIGEVANRFVQQNPHQLKKPLNSLSKGNKKSVVLLPHEQLENLLDKLSGYAKPE
ERILVLARYHHLKPQALSKAATRWPKLTIEFMTIHASKGQQAEYVIIAGLHQGNDGFPAVARESILEDVLLPQPEDFPDA
EERRLLYVALTRAKHQVWLLQDRERPSVFVEQLQDLGVPSQKKP
>Mature_684_residues
MELKATSLGKHLAQHPYNRVRLLHAGVEVSGEKHEYLIPFNQLIHVRCKRGIVWGELEFELPEEKVVRLHGTEWQETQRF
YHYLQQAWQQWSAEMSEVSAGVLQQQVEHIQRIEQQDKWFKKSELGKLQQQIRESFGALPMPVARLEQFDNCRDDYLLCQ
QWLQHGLQSVGQRNQQWTERMLESYHDFFQNIETSPLNDSQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRQEAE
PGQILLLAFGRQAAEEMNERIQERLGSVAIQAKTFHALALQIIQQGNRKVPAISKLETDSKARRALLITHWQQQCAEKKA
QAKGWRQWLLEELEWEVPEGDFWQDKRLAERLAGRLERWLGLMRMHGGSQAEMIEQADEEVRDLFSKRIRLMAPLLKAWK
GALKDEGAVDFSGLIHQAVNILEKGRFVSPWKHILVDEFQDISPQRAQLLAALRKQNKQTCLFTVGDDWQAIYRFSGAEL
TLTTAFAENFGEGAQCALDTTYRFNERIGEVANRFVQQNPHQLKKPLNSLSKGNKKSVVLLPHEQLENLLDKLSGYAKPE
ERILVLARYHHLKPQALSKAATRWPKLTIEFMTIHASKGQQAEYVIIAGLHQGNDGFPAVARESILEDVLLPQPEDFPDA
EERRLLYVALTRAKHQVWLLQDRERPSVFVEQLQDLGVPSQKKP

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=684, Percent_Identity=70.906432748538, Blast_Score=1023, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=338, Percent_Identity=27.810650887574, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI48994965, Length=341, Percent_Identity=28.7390029325513, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78657; Mature: 78657

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKATSLGKHLAQHPYNRVRLLHAGVEVSGEKHEYLIPFNQLIHVRCKRGIVWGELEFE
CCCCHHHHHHHHHHCCHHHEEHHEECCEECCCCCCEECCHHHHHHHHHHCCEEEEEEEEC
LPEEKVVRLHGTEWQETQRFYHYLQQAWQQWSAEMSEVSAGVLQQQVEHIQRIEQQDKWF
CCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSELGKLQQQIRESFGALPMPVARLEQFDNCRDDYLLCQQWLQHGLQSVGQRNQQWTER
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
MLESYHDFFQNIETSPLNDSQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRQEAE
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEHHHHHHHHCCCC
PGQILLLAFGRQAAEEMNERIQERLGSVAIQAKTFHALALQIIQQGNRKVPAISKLETDS
CCCEEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCH
KARRALLITHWQQQCAEKKAQAKGWRQWLLEELEWEVPEGDFWQDKRLAERLAGRLERWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GLMRMHGGSQAEMIEQADEEVRDLFSKRIRLMAPLLKAWKGALKDEGAVDFSGLIHQAVN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ILEKGRFVSPWKHILVDEFQDISPQRAQLLAALRKQNKQTCLFTVGDDWQAIYRFSGAEL
HHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
TLTTAFAENFGEGAQCALDTTYRFNERIGEVANRFVQQNPHQLKKPLNSLSKGNKKSVVL
EEEHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
LPHEQLENLLDKLSGYAKPEERILVLARYHHLKPQALSKAATRWPKLTIEFMTIHASKGQ
ECHHHHHHHHHHHCCCCCCHHHEEEEHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCC
QAEYVIIAGLHQGNDGFPAVARESILEDVLLPQPEDFPDAEERRLLYVALTRAKHQVWLL
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEHHCCCEEEEE
QDRERPSVFVEQLQDLGVPSQKKP
CCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MELKATSLGKHLAQHPYNRVRLLHAGVEVSGEKHEYLIPFNQLIHVRCKRGIVWGELEFE
CCCCHHHHHHHHHHCCHHHEEHHEECCEECCCCCCEECCHHHHHHHHHHCCEEEEEEEEC
LPEEKVVRLHGTEWQETQRFYHYLQQAWQQWSAEMSEVSAGVLQQQVEHIQRIEQQDKWF
CCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKSELGKLQQQIRESFGALPMPVARLEQFDNCRDDYLLCQQWLQHGLQSVGQRNQQWTER
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
MLESYHDFFQNIETSPLNDSQSRAVVNGEDSVLVLAGAGSGKTSVLVARAGWLLRRQEAE
HHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEEECCCCCCEEEEEHHHHHHHHCCCC
PGQILLLAFGRQAAEEMNERIQERLGSVAIQAKTFHALALQIIQQGNRKVPAISKLETDS
CCCEEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCH
KARRALLITHWQQQCAEKKAQAKGWRQWLLEELEWEVPEGDFWQDKRLAERLAGRLERWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
GLMRMHGGSQAEMIEQADEEVRDLFSKRIRLMAPLLKAWKGALKDEGAVDFSGLIHQAVN
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
ILEKGRFVSPWKHILVDEFQDISPQRAQLLAALRKQNKQTCLFTVGDDWQAIYRFSGAEL
HHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
TLTTAFAENFGEGAQCALDTTYRFNERIGEVANRFVQQNPHQLKKPLNSLSKGNKKSVVL
EEEHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEE
LPHEQLENLLDKLSGYAKPEERILVLARYHHLKPQALSKAATRWPKLTIEFMTIHASKGQ
ECHHHHHHHHHHHCCCCCCHHHEEEEHHHHCCCHHHHHHHHHCCCCEEEEEEEEECCCCC
QAEYVIIAGLHQGNDGFPAVARESILEDVLLPQPEDFPDAEERRLLYVALTRAKHQVWLL
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEEHHCCCEEEEE
QDRERPSVFVEQLQDLGVPSQKKP
CCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]