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The map label for this gene is aat

Identifier: 157369919

GI number: 157369919

Start: 1833463

End: 1834188

Strand: Reverse

Name: aat

Synonym: Spro_1676

Alternate gene names: 157369919

Gene position: 1834188-1833463 (Counterclockwise)

Preceding gene: 157369920

Following gene: 157369918

Centisome position: 33.66

GC content: 58.95

Gene sequence:

>726_bases
ATGCGCATAGTTAAGCTGTCACCGCATTCACTGGCGTTCCCCTCTCCTGAGGGCGCGCTGCGCGATCCTAACGGTTTGCT
GGCGATTGGCGGTGATTTAACCGCGCCGCGCTTGCTGGCCGCCTATGAACGCGGCATTTTCCCCTGGTTTTCACCGGGGG
AAGCCATTTTGTGGTGGTCGCCGGACCCACGCGCGGTGCTGTTCCCGGCCGAGCGCCATATCAGCCGCAGTCTGAAACGC
TTTCTGCGCAGTAACCCCTTTCGCGTGACGCTGAATCACGACTTTGCGGCGGTGATCGCCGCCTGCGCCGATCGACCGGA
AGAAGGCACCTGGATTGGCCCGGAAGTACAGCGCGCCTATTTGCATCTGCACCGCCTTGGCTTCGCCCATTCAATCGAGG
TATGGCAGGGCGATGAACTGGTCGGCGGCATGTACGGCGTGGCTCAGGGTGCGCTGTTTTGTGGCGAATCCATGTTTAGC
CGTACCACCAACGCTTCCAAGTGCGCCCTGATGACTTTTTGCCGCCATTTTGCCGCTTATGGCGGAGAATTGATTGACTG
TCAGGTGCTTAACGCTCACACTGCCACGCTGGGAGCGAAAGAGATCCCCCGAAGACAATTTTTGCAGCAGCTCAGCCAAC
TTCAGCGCAAGCCATTAGCGCCAGAATGCTGGGCACCGCAAGTGTTACCCCCACAGCAGGTTGAACCACCCTCCCCAACA
AACTAA

Upstream 100 bases:

>100_bases
TCTCACCCTGATGCGTCAACAGGGGCGTTATGCCCGGTTCCGCCAACGCATCAGTGGCATACCGCTGTAAAATGGCCTGT
AGAGGACGCTGAGACCTTTT

Downstream 100 bases:

>100_bases
TTTTGCGGAAACGGTGCAATGTTCACCGACACTTCTTTACATAATGTGGGTTTTTCGGCATTATCTTGCCGGTTAAAAAC
TAAGGTAGTTAGACCTAGAG

Product: leucyl/phenylalanyl-tRNA--protein transferase

Products: NA

Alternate protein names: L/F-transferase; Leucyltransferase; Phenyalanyltransferase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MRIVKLSPHSLAFPSPEGALRDPNGLLAIGGDLTAPRLLAAYERGIFPWFSPGEAILWWSPDPRAVLFPAERHISRSLKR
FLRSNPFRVTLNHDFAAVIAACADRPEEGTWIGPEVQRAYLHLHRLGFAHSIEVWQGDELVGGMYGVAQGALFCGESMFS
RTTNASKCALMTFCRHFAAYGGELIDCQVLNAHTATLGAKEIPRRQFLQQLSQLQRKPLAPECWAPQVLPPQQVEPPSPT
N

Sequences:

>Translated_241_residues
MRIVKLSPHSLAFPSPEGALRDPNGLLAIGGDLTAPRLLAAYERGIFPWFSPGEAILWWSPDPRAVLFPAERHISRSLKR
FLRSNPFRVTLNHDFAAVIAACADRPEEGTWIGPEVQRAYLHLHRLGFAHSIEVWQGDELVGGMYGVAQGALFCGESMFS
RTTNASKCALMTFCRHFAAYGGELIDCQVLNAHTATLGAKEIPRRQFLQQLSQLQRKPLAPECWAPQVLPPQQVEPPSPT
N
>Mature_241_residues
MRIVKLSPHSLAFPSPEGALRDPNGLLAIGGDLTAPRLLAAYERGIFPWFSPGEAILWWSPDPRAVLFPAERHISRSLKR
FLRSNPFRVTLNHDFAAVIAACADRPEEGTWIGPEVQRAYLHLHRLGFAHSIEVWQGDELVGGMYGVAQGALFCGESMFS
RTTNASKCALMTFCRHFAAYGGELIDCQVLNAHTATLGAKEIPRRQFLQQLSQLQRKPLAPECWAPQVLPPQQVEPPSPT
N

Specific function: Functions in the N-end rule pathway of protein degradation where it conjugates Leu, Phe and, less efficiently, Met from aminoacyl-tRNAs to the N-termini of proteins containing an N-terminal arginine or lysine

COG id: COG2360

COG function: function code O; Leu/Phe-tRNA-protein transferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the L/F-transferase family

Homologues:

Organism=Escherichia coli, GI1787111, Length=234, Percent_Identity=66.2393162393162, Blast_Score=317, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LFTR_SERP5 (A8GCE0)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477908.1
- ProteinModelPortal:   A8GCE0
- SMR:   A8GCE0
- STRING:   A8GCE0
- GeneID:   5604192
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1676
- eggNOG:   COG2360
- HOGENOM:   HBG485363
- OMA:   GEPILWW
- ProtClustDB:   PRK00301
- BioCyc:   SPRO399741:SPRO_1676-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00688
- InterPro:   IPR016181
- InterPro:   IPR004616
- TIGRFAMs:   TIGR00667

Pfam domain/function: PF03588 Leu_Phe_trans; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.2.6

Molecular weight: Translated: 26719; Mature: 26719

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVKLSPHSLAFPSPEGALRDPNGLLAIGGDLTAPRLLAAYERGIFPWFSPGEAILWWS
CEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEC
PDPRAVLFPAERHISRSLKRFLRSNPFRVTLNHDFAAVIAACADRPEEGTWIGPEVQRAY
CCCCEEEECCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHH
LHLHRLGFAHSIEVWQGDELVGGMYGVAQGALFCGESMFSRTTNASKCALMTFCRHFAAY
HHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GGELIDCQVLNAHTATLGAKEIPRRQFLQQLSQLQRKPLAPECWAPQVLPPQQVEPPSPT
CCCEEEEEEECCHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
N
C
>Mature Secondary Structure
MRIVKLSPHSLAFPSPEGALRDPNGLLAIGGDLTAPRLLAAYERGIFPWFSPGEAILWWS
CEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCEEEEEC
PDPRAVLFPAERHISRSLKRFLRSNPFRVTLNHDFAAVIAACADRPEEGTWIGPEVQRAY
CCCCEEEECCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHH
LHLHRLGFAHSIEVWQGDELVGGMYGVAQGALFCGESMFSRTTNASKCALMTFCRHFAAY
HHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
GGELIDCQVLNAHTATLGAKEIPRRQFLQQLSQLQRKPLAPECWAPQVLPPQQVEPPSPT
CCCEEEEEEECCHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA