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The map label for this gene is amiD [H]

Identifier: 157369902

GI number: 157369902

Start: 1812755

End: 1813591

Strand: Direct

Name: amiD [H]

Synonym: Spro_1659

Alternate gene names: 157369902

Gene position: 1812755-1813591 (Clockwise)

Preceding gene: 157369901

Following gene: 157369905

Centisome position: 33.27

GC content: 58.3

Gene sequence:

>837_bases
GTGAAAATTTGGCCAGGGATATTTGCCGTGGCGCTGCTCGCAGGCTGCCAAAGCATGCCGCAGAACACCATCGTCGATCA
TGGTGGCTATCAGCTAGAAACGTTGCATCAGGCCCAGGGCGCAGACCAGCGCATTCGTTTTCTGGTGATGCATTACACCG
CCGAAGACTTCCACTCCTCATTGAAAACCCTGACGGATGAACACGTCAGTGCGCATTATCTGTTACCGGCGCACCCGCCG
TTGGCTCAGGGCAAACCGGTGGCATTTCAACTGGTGCCGGAAGCTCTGCGAGCATGGCATGCCGGGGCCAGCAGTTGGCG
CGGCAGAACCAACCTCAATGACACCTCGATCGGTATTGAAATCGTCAATCGCGGATTCAACCAAACCCTGCTGTTTACCC
ACTGGCAGCCCTATACGCCACAGCAAATTGCCCTGTTGATCCCACTGAGCCGCGATATTATTCAGCGATACGGCATTCAG
CCGACGGACGTGGTGGGGCACAGCGATATTGCGCCGCAGCGCAAGCAGGATCCCGGTCCGTTATTCCCCTGGCAGCAATT
GGCGCAGGCGGGCATAGGTGCCTGGCCGGATGAGGGGCAGGTGCAGAAACTATTGGCCGGGCGGGATAAGCATGCCGAAG
TGCCGCTGGCACCGTTGATGGCCAAGCTGGCGAGTTACGGTTACGGCATGGATGCGCGCTGGGATGTCAGGCAGCAAAAA
AACGTGCTGGCGGCGTTTCAGATGCATTTCCGGCCCAGCGATTATCGCGGTGAGCCGGATGCGGAGAGTGAGGCGATTGT
CGATGCGCTGTTGCTGAAATACGGAGCCGCCCGTTAA

Upstream 100 bases:

>100_bases
GGCTGGCATCTCTGTCATCATTTATTGAATAACTTGATTATTGGATGCAAGTTAATCAATCCGGGTAACAAATTCGGCGT
AGCTAACACAGGAGGGATTC

Downstream 100 bases:

>100_bases
TTACAGGCCGTGCAGCTTGCCGTGATCCTTCAGCCAGCGGGCGGTACGCGAGATACCTTCATCCAGCGAAATAATTGGCC
GGTAATCCAGCTCTTGCTGG

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MKIWPGIFAVALLAGCQSMPQNTIVDHGGYQLETLHQAQGADQRIRFLVMHYTAEDFHSSLKTLTDEHVSAHYLLPAHPP
LAQGKPVAFQLVPEALRAWHAGASSWRGRTNLNDTSIGIEIVNRGFNQTLLFTHWQPYTPQQIALLIPLSRDIIQRYGIQ
PTDVVGHSDIAPQRKQDPGPLFPWQQLAQAGIGAWPDEGQVQKLLAGRDKHAEVPLAPLMAKLASYGYGMDARWDVRQQK
NVLAAFQMHFRPSDYRGEPDAESEAIVDALLLKYGAAR

Sequences:

>Translated_278_residues
MKIWPGIFAVALLAGCQSMPQNTIVDHGGYQLETLHQAQGADQRIRFLVMHYTAEDFHSSLKTLTDEHVSAHYLLPAHPP
LAQGKPVAFQLVPEALRAWHAGASSWRGRTNLNDTSIGIEIVNRGFNQTLLFTHWQPYTPQQIALLIPLSRDIIQRYGIQ
PTDVVGHSDIAPQRKQDPGPLFPWQQLAQAGIGAWPDEGQVQKLLAGRDKHAEVPLAPLMAKLASYGYGMDARWDVRQQK
NVLAAFQMHFRPSDYRGEPDAESEAIVDALLLKYGAAR
>Mature_278_residues
MKIWPGIFAVALLAGCQSMPQNTIVDHGGYQLETLHQAQGADQRIRFLVMHYTAEDFHSSLKTLTDEHVSAHYLLPAHPP
LAQGKPVAFQLVPEALRAWHAGASSWRGRTNLNDTSIGIEIVNRGFNQTLLFTHWQPYTPQQIALLIPLSRDIIQRYGIQ
PTDVVGHSDIAPQRKQDPGPLFPWQQLAQAGIGAWPDEGQVQKLLAGRDKHAEVPLAPLMAKLASYGYGMDARWDVRQQK
NVLAAFQMHFRPSDYRGEPDAESEAIVDALLLKYGAAR

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=261, Percent_Identity=58.6206896551724, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1786300, Length=117, Percent_Identity=38.4615384615385, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 30979; Mature: 30979

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIWPGIFAVALLAGCQSMPQNTIVDHGGYQLETLHQAQGADQRIRFLVMHYTAEDFHSS
CCCCHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCCCCHHEEEHHEECCHHHHHHH
LKTLTDEHVSAHYLLPAHPPLAQGKPVAFQLVPEALRAWHAGASSWRGRTNLNDTSIGIE
HHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
IVNRGFNQTLLFTHWQPYTPQQIALLIPLSRDIIQRYGIQPTDVVGHSDIAPQRKQDPGP
HCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LFPWQQLAQAGIGAWPDEGQVQKLLAGRDKHAEVPLAPLMAKLASYGYGMDARWDVRQQK
CCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
NVLAAFQMHFRPSDYRGEPDAESEAIVDALLLKYGAAR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIWPGIFAVALLAGCQSMPQNTIVDHGGYQLETLHQAQGADQRIRFLVMHYTAEDFHSS
CCCCHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHCCCCHHEEEHHEECCHHHHHHH
LKTLTDEHVSAHYLLPAHPPLAQGKPVAFQLVPEALRAWHAGASSWRGRTNLNDTSIGIE
HHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
IVNRGFNQTLLFTHWQPYTPQQIALLIPLSRDIIQRYGIQPTDVVGHSDIAPQRKQDPGP
HCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
LFPWQQLAQAGIGAWPDEGQVQKLLAGRDKHAEVPLAPLMAKLASYGYGMDARWDVRQQK
CCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
NVLAAFQMHFRPSDYRGEPDAESEAIVDALLLKYGAAR
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]