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The map label for this gene is potF [H]

Identifier: 157369888

GI number: 157369888

Start: 1801037

End: 1802146

Strand: Direct

Name: potF [H]

Synonym: Spro_1645

Alternate gene names: 157369888

Gene position: 1801037-1802146 (Clockwise)

Preceding gene: 157369887

Following gene: 157369889

Centisome position: 33.05

GC content: 54.14

Gene sequence:

>1110_bases
ATGGTCACCCTACGTAAAAAGTGGTTATCGGGTGTGGTTGCCGGCCTGCTGATGGCAGCAAGCGTCACGGCGTCAGCCGA
GGAAAAAACGCTGCACATCTATAACTGGTCCGACTATATTGCGCCGGACACCTTAGCCAAATTCCAGAAAGAAACCGGCA
TTAAAGTGGTGTACGACGTCTTTGACTCCAACGAAGTGTTGGAAGGCAAACTGATGGCGGGCAGCACCGGTTATGATCTG
GTAGTTCCTTCATCCAATTTCCTTGAGCGTCAGTCGAAGGCCGGTATTTTCGAGCCGCTCGACAAGAGCAAGATGCCGAA
CTACAAAAACCTTGATCCCGAGATGCTGCAACTGGTGGCGCACAATGACAAGGACAACAAGTACGGTATCCCTTATCTGA
TGGTGACCACCGGCATCGGCTATAACGTCGACAAGGTGAAGGCGGTACTGGGCAAAGACGCACCGGTTGACAGCTGGGAT
CTGATCCTCAAACCGGAAAATCTCGAGAAGTTGAAAAGCTGCGGCGTTTCCTTCCTGGATGCGCCAAGTGAGGTCTATGC
GACCGTGCTGCACTACCTGGGCAAGGATCCCAACAGCACCAACGCGGCAGATTACACCGGTGCGGCCAACGATCTGTTGC
TCAAGCTGCGGCCGAACATCCGTTATTTCCACTCTTCCCAGTACATCAACGATTTGGCAAACGGTGATATCTGCGTAGCG
ATCGGCTGGTCCGGCGACGTGATGCAGGCGGCCAATCGCGCCAAAGAAGCGAAGAACGGTGTGAACGTGGCCTATGCGAT
CCCGAAAGAGGGGGCGCTCACTTATTTTGACATGTTCGCCATGCCGGCGGATGCCAAAAACAAGGACGCTGCCTACCAGT
TCCTGAACTTCCTGATGAAGCCTGATGTCATGGCGGGGATCAGTAACTTTGTGTATTACGCCAACGCGGTGAAGGACTCT
ACACCGCTGGTCAATGCCGAAGTCCGCAACAATCCGAACGTGTACCCACCGGCCGATCTGCGCGCCAAGCTGTTTACGCT
GAACGTGCAGTCGCCGAAGCTGGATCGTGTGATTACCCGCGCCTGGACTAAAGTTAAAAGCGGACGCTAA

Upstream 100 bases:

>100_bases
CCGCGGTTGCAACGATTGCGCTCGATCAGGAAAGGTTTTGTATCGTTTGGCGGTACAAGTTCAATTCTATGATTCACCCC
TGTTTTGGAGGAAGTTACGG

Downstream 100 bases:

>100_bases
TACTCAGAGGGCGGCCGTTGGGCCGGTTTCAGGGTTGTTTTGACCGGCAGGCGGGTGCTCCCCGCCTGCATTGCCATTTT
GGGCCATGGCAATTGCCGTG

Product: putrescine ABC transporter periplasmic-binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MVTLRKKWLSGVVAGLLMAASVTASAEEKTLHIYNWSDYIAPDTLAKFQKETGIKVVYDVFDSNEVLEGKLMAGSTGYDL
VVPSSNFLERQSKAGIFEPLDKSKMPNYKNLDPEMLQLVAHNDKDNKYGIPYLMVTTGIGYNVDKVKAVLGKDAPVDSWD
LILKPENLEKLKSCGVSFLDAPSEVYATVLHYLGKDPNSTNAADYTGAANDLLLKLRPNIRYFHSSQYINDLANGDICVA
IGWSGDVMQAANRAKEAKNGVNVAYAIPKEGALTYFDMFAMPADAKNKDAAYQFLNFLMKPDVMAGISNFVYYANAVKDS
TPLVNAEVRNNPNVYPPADLRAKLFTLNVQSPKLDRVITRAWTKVKSGR

Sequences:

>Translated_369_residues
MVTLRKKWLSGVVAGLLMAASVTASAEEKTLHIYNWSDYIAPDTLAKFQKETGIKVVYDVFDSNEVLEGKLMAGSTGYDL
VVPSSNFLERQSKAGIFEPLDKSKMPNYKNLDPEMLQLVAHNDKDNKYGIPYLMVTTGIGYNVDKVKAVLGKDAPVDSWD
LILKPENLEKLKSCGVSFLDAPSEVYATVLHYLGKDPNSTNAADYTGAANDLLLKLRPNIRYFHSSQYINDLANGDICVA
IGWSGDVMQAANRAKEAKNGVNVAYAIPKEGALTYFDMFAMPADAKNKDAAYQFLNFLMKPDVMAGISNFVYYANAVKDS
TPLVNAEVRNNPNVYPPADLRAKLFTLNVQSPKLDRVITRAWTKVKSGR
>Mature_369_residues
MVTLRKKWLSGVVAGLLMAASVTASAEEKTLHIYNWSDYIAPDTLAKFQKETGIKVVYDVFDSNEVLEGKLMAGSTGYDL
VVPSSNFLERQSKAGIFEPLDKSKMPNYKNLDPEMLQLVAHNDKDNKYGIPYLMVTTGIGYNVDKVKAVLGKDAPVDSWD
LILKPENLEKLKSCGVSFLDAPSEVYATVLHYLGKDPNSTNAADYTGAANDLLLKLRPNIRYFHSSQYINDLANGDICVA
IGWSGDVMQAANRAKEAKNGVNVAYAIPKEGALTYFDMFAMPADAKNKDAAYQFLNFLMKPDVMAGISNFVYYANAVKDS
TPLVNAEVRNNPNVYPPADLRAKLFTLNVQSPKLDRVITRAWTKVKSGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=370, Percent_Identity=79.7297297297297, Blast_Score=619, Evalue=1e-179,
Organism=Escherichia coli, GI1787367, Length=320, Percent_Identity=35.625, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1787711, Length=300, Percent_Identity=25.3333333333333, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40749; Mature: 40749

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLRKKWLSGVVAGLLMAASVTASAEEKTLHIYNWSDYIAPDTLAKFQKETGIKVVYDV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
FDSNEVLEGKLMAGSTGYDLVVPSSNFLERQSKAGIFEPLDKSKMPNYKNLDPEMLQLVA
CCCCCEEECEEEECCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
HNDKDNKYGIPYLMVTTGIGYNVDKVKAVLGKDAPVDSWDLILKPENLEKLKSCGVSFLD
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCCHHC
APSEVYATVLHYLGKDPNSTNAADYTGAANDLLLKLRPNIRYFHSSQYINDLANGDICVA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHHHHCCCCEEEE
IGWSGDVMQAANRAKEAKNGVNVAYAIPKEGALTYFDMFAMPADAKNKDAAYQFLNFLMK
ECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHC
PDVMAGISNFVYYANAVKDSTPLVNAEVRNNPNVYPPADLRAKLFTLNVQSPKLDRVITR
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
AWTKVKSGR
HHHHHCCCC
>Mature Secondary Structure
MVTLRKKWLSGVVAGLLMAASVTASAEEKTLHIYNWSDYIAPDTLAKFQKETGIKVVYDV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCEEEEEE
FDSNEVLEGKLMAGSTGYDLVVPSSNFLERQSKAGIFEPLDKSKMPNYKNLDPEMLQLVA
CCCCCEEECEEEECCCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
HNDKDNKYGIPYLMVTTGIGYNVDKVKAVLGKDAPVDSWDLILKPENLEKLKSCGVSFLD
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHCCCCHHC
APSEVYATVLHYLGKDPNSTNAADYTGAANDLLLKLRPNIRYFHSSQYINDLANGDICVA
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHHHHCCCCEEEE
IGWSGDVMQAANRAKEAKNGVNVAYAIPKEGALTYFDMFAMPADAKNKDAAYQFLNFLMK
ECCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEHHHCCCCCCCCHHHHHHHHHHHHC
PDVMAGISNFVYYANAVKDSTPLVNAEVRNNPNVYPPADLRAKLFTLNVQSPKLDRVITR
CHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCHHHHHHHH
AWTKVKSGR
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]