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The map label for this gene is rimK

Identifier: 157369886

GI number: 157369886

Start: 1799085

End: 1799987

Strand: Direct

Name: rimK

Synonym: Spro_1643

Alternate gene names: 157369886

Gene position: 1799085-1799987 (Clockwise)

Preceding gene: 157369885

Following gene: 157369887

Centisome position: 33.02

GC content: 59.58

Gene sequence:

>903_bases
GTGAAAATTGCCATTCTTTCGCGCGATGGAACGTTGTACTCATGTAAACGCTTGGTGGAGGCGGCGGAACAGCGCGGCCA
CAGCATCGAGGTTATCGATCCACTCTCTTGCTATATGAACATCAACCCGGCCGCGCCGACTATCCACTACCGCGGCCGCC
AATTGGAGCGTTACGACGCGGTGATCCCGCGCATCGGCTCCGCCATGACCTTTTATGGCACTGCAGTGCTGCGCCAGTTT
GAACTGTTGGGCAGTTATCCGCTTAATGAGTCGGTGGCGATCACTCGTGCGCGCGACAAGCTGCGTTCGCTGCAGTTGCT
GGCACGGCAGGGGATCGATTTGCCGATCACCGGTTTTGCCCATTCGCCCGACGACACCGGCGATTTAATTGAACTGGTTG
GCGGTGCGCCGCTGGTGGTCAAACTGGTGGAAGGCACGCAGGGCATTGGCGTAGTACTGGCGGAAACTCGCCAGGCGGCA
GAAAGCGTGATTGATGCCTTTCGTGGCCTGAACGCTCATATTCTGGTGCAAGAATATATTCGCGAGGCGCAGGGCAGTGA
TGTGCGCTGCCTGGTGGTGGGGAGTAAGGTAGTCGCAGCCATTGAGCGCCAGGCCAAACCCGGGGAGTTTCGTTCCAATC
TGCACCGGGGTGGCACCGCCCGCAAGGTAAGCATCACTGCCAAAGAGCGGGCGATTGCGGTCAAGGCCGCCACCACACTG
GGGCTGGATGTCGCCGGGGTGGATATCCTGCGTGCGGCCCGTGGCCCGCTGGTGATGGAGGTTAATGCTTCACCGGGGTT
GGAAGGGGTGGAAAGTACCACCGGGTTGGATATCGCCGGCAGGATGATCGAGTATATCGAGCAGCGCGGCAGGCCGGGTT
TCAGGTTGAAATCTGGCGGATAA

Upstream 100 bases:

>100_bases
AGATACGCCTGAAGATAAAAACGACAGTGAGCCGCGTGCCTGAATAATGGCCACCGGAGACATACCTGCCAGACAGGGGC
TTGCCTTTTTGGGGTGACGA

Downstream 100 bases:

>100_bases
GTTTGCCGTAAAAAATTCGCCGTTCTGTGGCTGGAGTTGCGGCTTTTTATCGACCATATTTACCCTAATAGCGAATTAAG
CCGCATTGATCGTGGCGTAG

Product: alpha-L-glutamate ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MKIAILSRDGTLYSCKRLVEAAEQRGHSIEVIDPLSCYMNINPAAPTIHYRGRQLERYDAVIPRIGSAMTFYGTAVLRQF
ELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGFAHSPDDTGDLIELVGGAPLVVKLVEGTQGIGVVLAETRQAA
ESVIDAFRGLNAHILVQEYIREAQGSDVRCLVVGSKVVAAIERQAKPGEFRSNLHRGGTARKVSITAKERAIAVKAATTL
GLDVAGVDILRAARGPLVMEVNASPGLEGVESTTGLDIAGRMIEYIEQRGRPGFRLKSGG

Sequences:

>Translated_300_residues
MKIAILSRDGTLYSCKRLVEAAEQRGHSIEVIDPLSCYMNINPAAPTIHYRGRQLERYDAVIPRIGSAMTFYGTAVLRQF
ELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGFAHSPDDTGDLIELVGGAPLVVKLVEGTQGIGVVLAETRQAA
ESVIDAFRGLNAHILVQEYIREAQGSDVRCLVVGSKVVAAIERQAKPGEFRSNLHRGGTARKVSITAKERAIAVKAATTL
GLDVAGVDILRAARGPLVMEVNASPGLEGVESTTGLDIAGRMIEYIEQRGRPGFRLKSGG
>Mature_300_residues
MKIAILSRDGTLYSCKRLVEAAEQRGHSIEVIDPLSCYMNINPAAPTIHYRGRQLERYDAVIPRIGSAMTFYGTAVLRQF
ELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGFAHSPDDTGDLIELVGGAPLVVKLVEGTQGIGVVLAETRQAA
ESVIDAFRGLNAHILVQEYIREAQGSDVRCLVVGSKVVAAIERQAKPGEFRSNLHRGGTARKVSITAKERAIAVKAATTL
GLDVAGVDILRAARGPLVMEVNASPGLEGVESTTGLDIAGRMIEYIEQRGRPGFRLKSGG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI164663810, Length=214, Percent_Identity=29.9065420560748, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI134254462, Length=218, Percent_Identity=32.1100917431193, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1787076, Length=300, Percent_Identity=80.3333333333333, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMK_SERP5 (A8GCA7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477875.1
- STRING:   A8GCA7
- GeneID:   5604049
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1643
- eggNOG:   COG0189
- HOGENOM:   HBG679591
- OMA:   CREPRLY
- BioCyc:   SPRO399741:SPRO_1643-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01552
- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- TIGRFAMs:   TIGR00768

Pfam domain/function: PF08443 RimK

EC number: NA

Molecular weight: Translated: 32282; Mature: 32282

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAILSRDGTLYSCKRLVEAAEQRGHSIEVIDPLSCYMNINPAAPTIHYRGRQLERYDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCCEEEECCCHHHHHHH
VIPRIGSAMTFYGTAVLRQFELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGFA
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEECCC
HSPDDTGDLIELVGGAPLVVKLVEGTQGIGVVLAETRQAAESVIDAFRGLNAHILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEHHHHHH
REAQGSDVRCLVVGSKVVAAIERQAKPGEFRSNLHRGGTARKVSITAKERAIAVKAATTL
HHHCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEHHHEEEEEEHHHH
GLDVAGVDILRAARGPLVMEVNASPGLEGVESTTGLDIAGRMIEYIEQRGRPGFRLKSGG
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCC
>Mature Secondary Structure
MKIAILSRDGTLYSCKRLVEAAEQRGHSIEVIDPLSCYMNINPAAPTIHYRGRQLERYDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCCEEEECCCHHHHHHH
VIPRIGSAMTFYGTAVLRQFELLGSYPLNESVAITRARDKLRSLQLLARQGIDLPITGFA
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCEEECCC
HSPDDTGDLIELVGGAPLVVKLVEGTQGIGVVLAETRQAAESVIDAFRGLNAHILVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEHHHHHH
REAQGSDVRCLVVGSKVVAAIERQAKPGEFRSNLHRGGTARKVSITAKERAIAVKAATTL
HHHCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEHHHEEEEEEHHHH
GLDVAGVDILRAARGPLVMEVNASPGLEGVESTTGLDIAGRMIEYIEQRGRPGFRLKSGG
CCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA