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The map label for this gene is eco

Identifier: 157369880

GI number: 157369880

Start: 1793854

End: 1794366

Strand: Direct

Name: eco

Synonym: Spro_1637

Alternate gene names: 157369880

Gene position: 1793854-1794366 (Clockwise)

Preceding gene: 157369879

Following gene: 157369881

Centisome position: 32.92

GC content: 53.41

Gene sequence:

>513_bases
ATGAACAAGGCATCCGTTGTATTTTCCGGCCTGCTGATGGCCGTTTCCGCCAGCGCCATTGCTGCTACCTCGTCCGAAGA
CACCGATATCAGCAAGCAACCGCTGGAAAAGGTCGCCCCTTACCCGAAAGCCGAGAAGGGCATGAGCCGTCAGGTGATTT
ACCTGCCGAAGCAGGAACACGAAGAGAATTTCAAGGTTGAACTGCTGATTGGCAAAACTCTGGAAGTGGACTGTAACCGC
CACATGATTGGCGGTACGCTGGAAACCAAAACCCTTTCCGGTTGGGGTTACGACTACCTGGTAGTGGAAAAACTGTCTGA
GCCGGCTTCCACCATGATGGCTTGCCCGGACAATACCAAACAGCAGAAATTTATCGCTGCCAACCTGGGTGACGCTGCCA
TGCAACGTTACAACAGCCGCCTGCCAATCGTGGTCTACGCACCGAAAGACGTGGAAGTGAAATACCGCGTGTGGAAAGCC
GAAGACACCGTCAGCAAGGCAGAACAGAAGTAA

Upstream 100 bases:

>100_bases
TGGCCATTGCATATTGTAAAGAAGATTAAACTACTGGCGAGCTTGCCGGTAGTGTATTAGCTTATGCCCGGAGCATTTAA
TAAATTGTAGGGATATAAAC

Downstream 100 bases:

>100_bases
TACCGATGCGTGCTAACCATTAGCACGGCTAAGTGATTACCCGAAAATGAACCCGTCTGGAGAGTGCATTGCGCCGTTGG
CCATTGCCCCTTGGACGGGT

Product: ecotin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MNKASVVFSGLLMAVSASAIAATSSEDTDISKQPLEKVAPYPKAEKGMSRQVIYLPKQEHEENFKVELLIGKTLEVDCNR
HMIGGTLETKTLSGWGYDYLVVEKLSEPASTMMACPDNTKQQKFIAANLGDAAMQRYNSRLPIVVYAPKDVEVKYRVWKA
EDTVSKAEQK

Sequences:

>Translated_170_residues
MNKASVVFSGLLMAVSASAIAATSSEDTDISKQPLEKVAPYPKAEKGMSRQVIYLPKQEHEENFKVELLIGKTLEVDCNR
HMIGGTLETKTLSGWGYDYLVVEKLSEPASTMMACPDNTKQQKFIAANLGDAAMQRYNSRLPIVVYAPKDVEVKYRVWKA
EDTVSKAEQK
>Mature_170_residues
MNKASVVFSGLLMAVSASAIAATSSEDTDISKQPLEKVAPYPKAEKGMSRQVIYLPKQEHEENFKVELLIGKTLEVDCNR
HMIGGTLETKTLSGWGYDYLVVEKLSEPASTMMACPDNTKQQKFIAANLGDAAMQRYNSRLPIVVYAPKDVEVKYRVWKA
EDTVSKAEQK

Specific function: General inhibitor of pancreatic serine proteases:inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases

COG id: COG4574

COG function: function code R; Serine protease inhibitor ecotin

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the protease inhibitor I11 (ecotin) family

Homologues:

Organism=Escherichia coli, GI1788538, Length=157, Percent_Identity=64.3312101910828, Blast_Score=209, Evalue=6e-56,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): ECOT_SERP5 (A8GCA1)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477869.1
- ProteinModelPortal:   A8GCA1
- SMR:   A8GCA1
- STRING:   A8GCA1
- MEROPS:   I11.001
- GeneID:   5604000
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1637
- eggNOG:   COG4574
- HOGENOM:   HBG710412
- OMA:   RYDSRLP
- ProtClustDB:   PRK03719
- BioCyc:   SPRO399741:SPRO_1637-MONOMER
- HAMAP:   MF_00706
- InterPro:   IPR005658
- InterPro:   IPR023189
- InterPro:   IPR023084
- Gene3D:   G3DSA:2.60.40.550
- PIRSF:   PIRSF006865

Pfam domain/function: PF03974 Ecotin; SSF49772 Prot_inh_ecotin

EC number: NA

Molecular weight: Translated: 18877; Mature: 18877

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKASVVFSGLLMAVSASAIAATSSEDTDISKQPLEKVAPYPKAEKGMSRQVIYLPKQEH
CCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCC
EENFKVELLIGKTLEVDCNRHMIGGTLETKTLSGWGYDYLVVEKLSEPASTMMACPDNTK
CCCCEEEEEECCEEEECCCCEEECCEEEEEEECCCCCEEEEEEHHCCCCHHEEECCCCCC
QQKFIAANLGDAAMQRYNSRLPIVVYAPKDVEVKYRVWKAEDTVSKAEQK
CCEEEEECCCHHHHHHHCCCCCEEEECCCCCEEEEEEEECCHHHHHHCCC
>Mature Secondary Structure
MNKASVVFSGLLMAVSASAIAATSSEDTDISKQPLEKVAPYPKAEKGMSRQVIYLPKQEH
CCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEECCCCCC
EENFKVELLIGKTLEVDCNRHMIGGTLETKTLSGWGYDYLVVEKLSEPASTMMACPDNTK
CCCCEEEEEECCEEEECCCCEEECCEEEEEEECCCCCEEEEEEHHCCCCHHEEECCCCCC
QQKFIAANLGDAAMQRYNSRLPIVVYAPKDVEVKYRVWKAEDTVSKAEQK
CCEEEEECCCHHHHHHHCCCCCEEEECCCCCEEEEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA