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The map label for this gene is iaaA [H]

Identifier: 157369792

GI number: 157369792

Start: 1687842

End: 1688789

Strand: Direct

Name: iaaA [H]

Synonym: Spro_1549

Alternate gene names: 157369792

Gene position: 1687842-1688789 (Clockwise)

Preceding gene: 157369791

Following gene: 157369793

Centisome position: 30.98

GC content: 60.97

Gene sequence:

>948_bases
ATGAGCAAACCAGTGATTGCCATTCATGGCGGCGCGGGCGCAATTACCCGCTCGGCGCTGAGTGCGGAAAAGGAACAGGA
GTATATCCAGGCGTTATCCGGCATTGTTGCCGCAGGTCAGCAGATCCTGGCCCAGGGCGGCAACGCATTGGATGCGGTGA
CCGAAGCCGTGCGCTTGCTGGAAGAGTGCCCGTTATTTAATGCCGGCAAAGGCTCGGTGTTTACTCATCAGGGTACCCAT
GAGCTGGACGCCTGCGTGATGGACGGGCGCACCTGCGATGCCGGCGCGGTGGCGGGCGTCAGCCGGATACGCAACCCGAT
ACTGGCCGCACGCGCGGTGCTGGAAAACAGCCAGCATGTGTTGTTTGCCGGTGAGGGCGCTGAAAAGTTTGCCGCGGCCC
ACGGTCTTGAAATGGTTACCCCCGATTTCTTCTTTACCCAACAACGTTTTGACCAGTTGCACCGCGCTCAGGCGGAGCAG
GGCCGAGTATTGCTCGATCACGATGGCGCTGAGCCGATCGATCCCGACAGGAAGTTCGGTACCGTCGGTGCGGTGGCGCT
GGATGCTTTGGGCAATTTGGCGGCGGCGACATCGACCGGTGGCATGACCAACAAACAGGCCGGTCGGGTGGGCGATACCC
CGATCATCGGCGCCGGCTGTTATGCCAACAATGCCACCGTGGCGGTGTCCAGCACCGGTACCGGCGAAATATTCATGCGT
GGCGTATCGGCCTATGACGTTTCCGCGCTGATGGAATACGCCGGCCTCAGCCTGCAGCAGGCCAGCGACCGGGTGGTAAT
GGAAAAACTGCTGGCGATGGGCGGTAGCGGCGGCATGATTGCCATCGACAGCCAGGGCAACGTGGCGCTGCCGTTTAACA
GCGAGGGGATGTACCGCGGTTTTGGTTATGTCGGCGATGCGCCGTCCGTAGGGATTTATACCCTATAG

Upstream 100 bases:

>100_bases
ATGCTTTACATAGAATGCAGGGCTAAATATAGTCGGGAAAAGAAAAAATAATTAATTATCAGCCCGGAAGGGTACGGGAT
TAACACAGGGTGTCAGGCAT

Downstream 100 bases:

>100_bases
CTTTCAAGTTGCCGCGAGCGCAGATAACAACGCTGCAACTTGAAATATGACGGGTATATCGCTAACCGTTCACCAGGAGG
GTACATGACCGATGCCTTAA

Product: peptidase T2 asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSKPVIAIHGGAGAITRSALSAEKEQEYIQALSGIVAAGQQILAQGGNALDAVTEAVRLLEECPLFNAGKGSVFTHQGTH
ELDACVMDGRTCDAGAVAGVSRIRNPILAARAVLENSQHVLFAGEGAEKFAAAHGLEMVTPDFFFTQQRFDQLHRAQAEQ
GRVLLDHDGAEPIDPDRKFGTVGAVALDALGNLAAATSTGGMTNKQAGRVGDTPIIGAGCYANNATVAVSSTGTGEIFMR
GVSAYDVSALMEYAGLSLQQASDRVVMEKLLAMGGSGGMIAIDSQGNVALPFNSEGMYRGFGYVGDAPSVGIYTL

Sequences:

>Translated_315_residues
MSKPVIAIHGGAGAITRSALSAEKEQEYIQALSGIVAAGQQILAQGGNALDAVTEAVRLLEECPLFNAGKGSVFTHQGTH
ELDACVMDGRTCDAGAVAGVSRIRNPILAARAVLENSQHVLFAGEGAEKFAAAHGLEMVTPDFFFTQQRFDQLHRAQAEQ
GRVLLDHDGAEPIDPDRKFGTVGAVALDALGNLAAATSTGGMTNKQAGRVGDTPIIGAGCYANNATVAVSSTGTGEIFMR
GVSAYDVSALMEYAGLSLQQASDRVVMEKLLAMGGSGGMIAIDSQGNVALPFNSEGMYRGFGYVGDAPSVGIYTL
>Mature_314_residues
SKPVIAIHGGAGAITRSALSAEKEQEYIQALSGIVAAGQQILAQGGNALDAVTEAVRLLEECPLFNAGKGSVFTHQGTHE
LDACVMDGRTCDAGAVAGVSRIRNPILAARAVLENSQHVLFAGEGAEKFAAAHGLEMVTPDFFFTQQRFDQLHRAQAEQG
RVLLDHDGAEPIDPDRKFGTVGAVALDALGNLAAATSTGGMTNKQAGRVGDTPIIGAGCYANNATVAVSSTGTGEIFMRG
VSAYDVSALMEYAGLSLQQASDRVVMEKLLAMGGSGGMIAIDSQGNVALPFNSEGMYRGFGYVGDAPSVGIYTL

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=295, Percent_Identity=38.3050847457627, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI145275200, Length=295, Percent_Identity=38.3050847457627, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI98991769, Length=322, Percent_Identity=30.1242236024845, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI285002251, Length=255, Percent_Identity=34.9019607843137, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI285002253, Length=255, Percent_Identity=32.9411764705882, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787050, Length=313, Percent_Identity=67.4121405750799, Blast_Score=427, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI32564649, Length=320, Percent_Identity=24.0625, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI18921183, Length=321, Percent_Identity=38.006230529595, Blast_Score=182, Evalue=2e-46,
Organism=Drosophila melanogaster, GI21357893, Length=269, Percent_Identity=33.457249070632, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI221330102, Length=252, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24652135, Length=252, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 32567; Mature: 32436

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPVIAIHGGAGAITRSALSAEKEQEYIQALSGIVAAGQQILAQGGNALDAVTEAVRLL
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EECPLFNAGKGSVFTHQGTHELDACVMDGRTCDAGAVAGVSRIRNPILAARAVLENSQHV
HHCCCCCCCCCCEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
LFAGEGAEKFAAAHGLEMVTPDFFFTQQRFDQLHRAQAEQGRVLLDHDGAEPIDPDRKFG
EEECCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
TVGAVALDALGNLAAATSTGGMTNKQAGRVGDTPIIGAGCYANNATVAVSSTGTGEIFMR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCEECCCCEEEEECCCCCEEEEE
GVSAYDVSALMEYAGLSLQQASDRVVMEKLLAMGGSGGMIAIDSQGNVALPFNSEGMYRG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCC
FGYVGDAPSVGIYTL
CCCCCCCCCCCCEEC
>Mature Secondary Structure 
SKPVIAIHGGAGAITRSALSAEKEQEYIQALSGIVAAGQQILAQGGNALDAVTEAVRLL
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EECPLFNAGKGSVFTHQGTHELDACVMDGRTCDAGAVAGVSRIRNPILAARAVLENSQHV
HHCCCCCCCCCCEEECCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
LFAGEGAEKFAAAHGLEMVTPDFFFTQQRFDQLHRAQAEQGRVLLDHDGAEPIDPDRKFG
EEECCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
TVGAVALDALGNLAAATSTGGMTNKQAGRVGDTPIIGAGCYANNATVAVSSTGTGEIFMR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCEECCCCEEEEECCCCCEEEEE
GVSAYDVSALMEYAGLSLQQASDRVVMEKLLAMGGSGGMIAIDSQGNVALPFNSEGMYRG
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEEECCCCCCCC
FGYVGDAPSVGIYTL
CCCCCCCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]