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The map label for this gene is braD [H]

Identifier: 157369665

GI number: 157369665

Start: 1553948

End: 1555528

Strand: Direct

Name: braD [H]

Synonym: Spro_1422

Alternate gene names: 157369665

Gene position: 1553948-1555528 (Clockwise)

Preceding gene: 157369664

Following gene: 157369666

Centisome position: 28.52

GC content: 62.05

Gene sequence:

>1581_bases
ATGAAACTTTTATCTTTAAGCCATCTCAGGCATCTGTGGTTATTGCTGTGCTGCCTGCCCATGTTCGCGCAGGCGGGGCT
GGCCGATGATTTTGCTGCGGCCAACCGTACGCAGCAGGCCAAATTATTGCAGGCGTGGGCGAGCGCGCCGGACGCTGCGC
GTTTGCCGTTACTGCAGGCGTTAAAGCAGGAAAACCTGATGATCGACGAGGCCAAACATGCCTTTATCCAGCAGGGCGAT
CGCTATCAGCCACTGGAGGGTGGCGCTGCCCCGGTCGGTACGCCGAAGAAGCTTTGGCTGAATAACCGGCTGCGGATCCT
GATTGCCAACGCGTTGTCGGCACAGCAGCTGGTCAGTGAAGATCCGGCGCTGCGTTTACAGGCGGCGCAGGCCCTGCAGC
AGGAGGCGAAAAACAGCCAGTTACCGCTGTTGACCCAGCGCCTGGCGCAGGAAAAAGACGACCGGGTGCATGAGGCGCTC
CGCATCGCGCTGGCCAATCTGCAACTGGGCGACGCCGATGCCAAAGTGCGTCTCAATGCGGTGCGGCTGCTCGGCAGTTC
GGGGGCAGCGGAAACCCAGACGCGGCTGCAGGCGATGACCGACGCCGCTCATGAACCGGACGCCGCCGTGCGAGCCGAGG
CGTTGAAAAGCCTGACCAGCGTGAAACACCGGCTGATGATCGGCGATCTGATTGGCCAGGCGTTTACCGGCCTGTCGCTT
GGCTCGATCCTGCTGTTGGCGGCGCTCGGGCTGGCGATCACCTACGGCCTGCTGGGGGTGATCAACATGGCGCACGGCGA
AATGCTGATGCTCGGTGCCTACTCGGCCTATCTGGTGCAGGGGCTGTTTCAGCAGTTTGCGCCGCAGTGGCTGGCACTGT
ATCCGCTGGTGGCGTTGCCGGTGGCGTTTGGCATCACCGCCTGCATAGGTATGGCGCTGGAGCGGACGGTGATCCGCCAT
CTGTATGGGCGGCCGCTGGAAACCCTGCTGGCGACCTGGGGCATCAGCCTGGTGCTGATCCAATGTGTTCGGGTGCTATT
TGGCGCGCAAAACGTGGAGGTTGCCAACCCGGCCTGGCTGTCGGGCGGCATTCAGTTACTGCCCAATCTGGTGCTGCCGT
GGAACCGGATCGCGGTGATCCTGTTTGTGGTGCTGGTACTGGTGCTCACCTGGCTGCTGCTGAACAAAACCCGCTTGGGT
ATGAACGTGCGCGCCGTGACGCAAAACCGTGCGATGGCCGCCTGCTGCGGCGTACCCACCGGCCGCGTCGATATGCTGGC
GTTCGGCCTGGGTTCCGGCATTGCCGGGCTGGGCGGAGTGGCGTTGTCGCAGCTGGGCAACGTCGGGCCGGAGCTGGGCC
AGGGTTATATCATCGACTCGTTCCTGGTAGTGGTGCTCGGCGGCGTTGGCCAACTGGCCGGGACCGTGGTGGCGGCCTTT
GGCCTGGGTATTCTTAACAAAATCCTTGAACCGCAGATTGGGGCCGTGCTGGGTAAAATCCTGATCCTGGCGCTGATCGT
GTTGTTTATTCAAAAACGTCCTCAGGGACTGTTCGCCTTCAAGGGCAGGGTGATTGACTGA

Upstream 100 bases:

>100_bases
GGGAACATGATCACCTTAACTGACAAGCATTAGGCAAGAATGCCCGGCCCCGTTACGGCGGGGGCTGACTGAGCCATGCC
CTATTGCGACGAGACATCTG

Downstream 100 bases:

>100_bases
TGAGCCAACCATTGACTGTAACCGGCCTGCAGAAGGCACCCCGACTGGCGCTGGGCATCGGCGGGCTGGCGTTGCTGGTA
CTGTTGATCATGCCGTTTCT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MKLLSLSHLRHLWLLLCCLPMFAQAGLADDFAAANRTQQAKLLQAWASAPDAARLPLLQALKQENLMIDEAKHAFIQQGD
RYQPLEGGAAPVGTPKKLWLNNRLRILIANALSAQQLVSEDPALRLQAAQALQQEAKNSQLPLLTQRLAQEKDDRVHEAL
RIALANLQLGDADAKVRLNAVRLLGSSGAAETQTRLQAMTDAAHEPDAAVRAEALKSLTSVKHRLMIGDLIGQAFTGLSL
GSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQGLFQQFAPQWLALYPLVALPVAFGITACIGMALERTVIRH
LYGRPLETLLATWGISLVLIQCVRVLFGAQNVEVANPAWLSGGIQLLPNLVLPWNRIAVILFVVLVLVLTWLLLNKTRLG
MNVRAVTQNRAMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGTVVAAF
GLGILNKILEPQIGAVLGKILILALIVLFIQKRPQGLFAFKGRVID

Sequences:

>Translated_526_residues
MKLLSLSHLRHLWLLLCCLPMFAQAGLADDFAAANRTQQAKLLQAWASAPDAARLPLLQALKQENLMIDEAKHAFIQQGD
RYQPLEGGAAPVGTPKKLWLNNRLRILIANALSAQQLVSEDPALRLQAAQALQQEAKNSQLPLLTQRLAQEKDDRVHEAL
RIALANLQLGDADAKVRLNAVRLLGSSGAAETQTRLQAMTDAAHEPDAAVRAEALKSLTSVKHRLMIGDLIGQAFTGLSL
GSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQGLFQQFAPQWLALYPLVALPVAFGITACIGMALERTVIRH
LYGRPLETLLATWGISLVLIQCVRVLFGAQNVEVANPAWLSGGIQLLPNLVLPWNRIAVILFVVLVLVLTWLLLNKTRLG
MNVRAVTQNRAMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGTVVAAF
GLGILNKILEPQIGAVLGKILILALIVLFIQKRPQGLFAFKGRVID
>Mature_526_residues
MKLLSLSHLRHLWLLLCCLPMFAQAGLADDFAAANRTQQAKLLQAWASAPDAARLPLLQALKQENLMIDEAKHAFIQQGD
RYQPLEGGAAPVGTPKKLWLNNRLRILIANALSAQQLVSEDPALRLQAAQALQQEAKNSQLPLLTQRLAQEKDDRVHEAL
RIALANLQLGDADAKVRLNAVRLLGSSGAAETQTRLQAMTDAAHEPDAAVRAEALKSLTSVKHRLMIGDLIGQAFTGLSL
GSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQGLFQQFAPQWLALYPLVALPVAFGITACIGMALERTVIRH
LYGRPLETLLATWGISLVLIQCVRVLFGAQNVEVANPAWLSGGIQLLPNLVLPWNRIAVILFVVLVLVLTWLLLNKTRLG
MNVRAVTQNRAMAACCGVPTGRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGTVVAAF
GLGILNKILEPQIGAVLGKILILALIVLFIQKRPQGLFAFKGRVID

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=294, Percent_Identity=28.2312925170068, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 56272; Mature: 56272

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLSLSHLRHLWLLLCCLPMFAQAGLADDFAAANRTQQAKLLQAWASAPDAARLPLLQA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHH
LKQENLMIDEAKHAFIQQGDRYQPLEGGAAPVGTPKKLWLNNRLRILIANALSAQQLVSE
HHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEHHHHHHHHHCC
DPALRLQAAQALQQEAKNSQLPLLTQRLAQEKDDRVHEALRIALANLQLGDADAKVRLNA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VRLLGSSGAAETQTRLQAMTDAAHEPDAAVRAEALKSLTSVKHRLMIGDLIGQAFTGLSL
HHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQGLFQQFAPQWLALYPLVALP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VAFGITACIGMALERTVIRHLYGRPLETLLATWGISLVLIQCVRVLFGAQNVEVANPAWL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
SGGIQLLPNLVLPWNRIAVILFVVLVLVLTWLLLNKTRLGMNVRAVTQNRAMAACCGVPT
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHHHCCCC
GRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGTVVAAF
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
GLGILNKILEPQIGAVLGKILILALIVLFIQKRPQGLFAFKGRVID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCC
>Mature Secondary Structure
MKLLSLSHLRHLWLLLCCLPMFAQAGLADDFAAANRTQQAKLLQAWASAPDAARLPLLQA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHH
LKQENLMIDEAKHAFIQQGDRYQPLEGGAAPVGTPKKLWLNNRLRILIANALSAQQLVSE
HHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEEHHHHHHHHHCC
DPALRLQAAQALQQEAKNSQLPLLTQRLAQEKDDRVHEALRIALANLQLGDADAKVRLNA
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
VRLLGSSGAAETQTRLQAMTDAAHEPDAAVRAEALKSLTSVKHRLMIGDLIGQAFTGLSL
HHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GSILLLAALGLAITYGLLGVINMAHGEMLMLGAYSAYLVQGLFQQFAPQWLALYPLVALP
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VAFGITACIGMALERTVIRHLYGRPLETLLATWGISLVLIQCVRVLFGAQNVEVANPAWL
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
SGGIQLLPNLVLPWNRIAVILFVVLVLVLTWLLLNKTRLGMNVRAVTQNRAMAACCGVPT
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHHHCCCC
GRVDMLAFGLGSGIAGLGGVALSQLGNVGPELGQGYIIDSFLVVVLGGVGQLAGTVVAAF
CCHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHH
GLGILNKILEPQIGAVLGKILILALIVLFIQKRPQGLFAFKGRVID
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]