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The map label for this gene is phnD [H]

Identifier: 157369654

GI number: 157369654

Start: 1537665

End: 1538597

Strand: Direct

Name: phnD [H]

Synonym: Spro_1411

Alternate gene names: 157369654

Gene position: 1537665-1538597 (Clockwise)

Preceding gene: 157369653

Following gene: 157369655

Centisome position: 28.22

GC content: 54.34

Gene sequence:

>933_bases
ATGAAAAAAGTATTGAGTCTGACCACCCTGATGGCCGGCGCGATGATGGTATTTAACGCTACTGCAGCGGACGCACCCAA
AGTATTGAATCTGGGTATTCTCGGCGGGCAAAACGCTACCCAGCAAATTGGTGATAACCAATGCGTTAAACAGTTCCTGG
ACAAGGAGCTTGGCGTTGACACCAAGCTGCGTAACTCCTCCGACTATTCCGGCGTCATTCAGGGCCTGTTGGGCGGCAAG
ATCGACCTGGTGTTGAGCATGTCACCGTCGTCGTTTGCCTCGGTGTACATCAAGGATCCGAAAGCGGTGGATATCGTCGG
CATCGCGGTTGATGACGTCGACCAATCCCGTGGTTACCACTCGGTGGTGATCGTTAAAGCCGGTAGTCCATATAAAAAGC
TGGAAGACCTGAAAGGCAAGGCCGTGGGCTTCGCCGATCCGGATTCTACCTCCGGCTTCCTGATCCCGAACCAGGAATTC
AAGAAACTGTTTGGCGGCACGGTAGACAACAAATACAACAATACTTTCTCCAGCGTGACCTTCTCCGGTGGCCATGAGCA
GGACGTCCTCGGCGTACTGAACGGTCAGTTTGAAGGCGCAGTGACCTGGGCGTCGATGATCGGCGACTACAACACCGGTT
ATACCAGCGGCGCGTTCACCCGCATGATCCGTATGGATCACCCGGACCTGATGAAGCAGATCCGCATTATCTGGCAGTCG
CCGCTGATCCCGAACGGTCCAATCCTGGTGAGCAACGCGCTGCCGGCAGAGTTTAAAGCCAAAGTCGTCACCGCGATCAA
GAAGCTGGATAAGGACGATCACCAGTGCTTTATCAAAGCGATGGGCGGCAAACAGCACATCGGCGAGACCACCCTGGCGG
AATACCAGAACATCATCGATATGAAGCGTGAGCTGACCAAAGGCGACCGTTAA

Upstream 100 bases:

>100_bases
GCAACTGAACGAACGGATACTGCACCAGCTGTATGGCGAGGAAGCCAATCAAATCCATTGAGTATTTCTTCACTTTTACC
TTTACACACAAGGCAGCGTA

Downstream 100 bases:

>100_bases
TCATCGGATTACGTCCTGATCGATCCAAAGGGGTTCAGCGTGCTGAACCCCTTGTTCCATAAGATGGAAATCTATTTTGA
ATAAAGATTTTGCGCACTAT

Product: phosphonate ABC transporter periplasmic phosphonate-binding protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MKKVLSLTTLMAGAMMVFNATAADAPKVLNLGILGGQNATQQIGDNQCVKQFLDKELGVDTKLRNSSDYSGVIQGLLGGK
IDLVLSMSPSSFASVYIKDPKAVDIVGIAVDDVDQSRGYHSVVIVKAGSPYKKLEDLKGKAVGFADPDSTSGFLIPNQEF
KKLFGGTVDNKYNNTFSSVTFSGGHEQDVLGVLNGQFEGAVTWASMIGDYNTGYTSGAFTRMIRMDHPDLMKQIRIIWQS
PLIPNGPILVSNALPAEFKAKVVTAIKKLDKDDHQCFIKAMGGKQHIGETTLAEYQNIIDMKRELTKGDR

Sequences:

>Translated_310_residues
MKKVLSLTTLMAGAMMVFNATAADAPKVLNLGILGGQNATQQIGDNQCVKQFLDKELGVDTKLRNSSDYSGVIQGLLGGK
IDLVLSMSPSSFASVYIKDPKAVDIVGIAVDDVDQSRGYHSVVIVKAGSPYKKLEDLKGKAVGFADPDSTSGFLIPNQEF
KKLFGGTVDNKYNNTFSSVTFSGGHEQDVLGVLNGQFEGAVTWASMIGDYNTGYTSGAFTRMIRMDHPDLMKQIRIIWQS
PLIPNGPILVSNALPAEFKAKVVTAIKKLDKDDHQCFIKAMGGKQHIGETTLAEYQNIIDMKRELTKGDR
>Mature_310_residues
MKKVLSLTTLMAGAMMVFNATAADAPKVLNLGILGGQNATQQIGDNQCVKQFLDKELGVDTKLRNSSDYSGVIQGLLGGK
IDLVLSMSPSSFASVYIKDPKAVDIVGIAVDDVDQSRGYHSVVIVKAGSPYKKLEDLKGKAVGFADPDSTSGFLIPNQEF
KKLFGGTVDNKYNNTFSSVTFSGGHEQDVLGVLNGQFEGAVTWASMIGDYNTGYTSGAFTRMIRMDHPDLMKQIRIIWQS
PLIPNGPILVSNALPAEFKAKVVTAIKKLDKDDHQCFIKAMGGKQHIGETTLAEYQNIIDMKRELTKGDR

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=266, Percent_Identity=31.203007518797, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 33588; Mature: 33588

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLSLTTLMAGAMMVFNATAADAPKVLNLGILGGQNATQQIGDNQCVKQFLDKELGVD
CCHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCHHHHCCCHHHHHHHHHHHHCCC
TKLRNSSDYSGVIQGLLGGKIDLVLSMSPSSFASVYIKDPKAVDIVGIAVDDVDQSRGYH
CCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCEEEEEEEEEECCHHCCCCE
SVVIVKAGSPYKKLEDLKGKAVGFADPDSTSGFLIPNQEFKKLFGGTVDNKYNNTFSSVT
EEEEEECCCCHHHHHHCCCCEEECCCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCEEE
FSGGHEQDVLGVLNGQFEGAVTWASMIGDYNTGYTSGAFTRMIRMDHPDLMKQIRIIWQS
ECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCC
PLIPNGPILVSNALPAEFKAKVVTAIKKLDKDDHQCFIKAMGGKQHIGETTLAEYQNIID
CCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHCCHHHHHHHHHHHH
MKRELTKGDR
HHHHHHCCCC
>Mature Secondary Structure
MKKVLSLTTLMAGAMMVFNATAADAPKVLNLGILGGQNATQQIGDNQCVKQFLDKELGVD
CCHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCHHHHCCCHHHHHHHHHHHHCCC
TKLRNSSDYSGVIQGLLGGKIDLVLSMSPSSFASVYIKDPKAVDIVGIAVDDVDQSRGYH
CCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCEEEEEEEEEECCHHCCCCE
SVVIVKAGSPYKKLEDLKGKAVGFADPDSTSGFLIPNQEFKKLFGGTVDNKYNNTFSSVT
EEEEEECCCCHHHHHHCCCCEEECCCCCCCCCEECCCHHHHHHHCCCCCCCCCCCCCEEE
FSGGHEQDVLGVLNGQFEGAVTWASMIGDYNTGYTSGAFTRMIRMDHPDLMKQIRIIWQS
ECCCCCHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCC
PLIPNGPILVSNALPAEFKAKVVTAIKKLDKDDHQCFIKAMGGKQHIGETTLAEYQNIID
CCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCHHEEEECCCCCHHCCHHHHHHHHHHHH
MKRELTKGDR
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]