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The map label for this gene is amyA [H]

Identifier: 157369630

GI number: 157369630

Start: 1515543

End: 1517033

Strand: Reverse

Name: amyA [H]

Synonym: Spro_1387

Alternate gene names: 157369630

Gene position: 1517033-1515543 (Counterclockwise)

Preceding gene: 157369631

Following gene: 157369629

Centisome position: 27.84

GC content: 55.73

Gene sequence:

>1491_bases
ATGACGAAAAAAACCACGTTGCTGCAGTTTTTCCATTGGTACTACCCCGATGGCGGTAAGTTATGGCAGGAAGCCGCCGA
ACGCGCCGCGCAAATTGCGCAGTTGGGCATCACCGATCTGTGGCTGCCCCCCGCTTATAAGGGGGCTTCCGGCGGCTATT
CGGTGGGTTACGACAGTTATGATCTGTTCGATCTGGGCGAGTTTGATCAAAAAGGCAGCCGTGCCACCAAATACGGCGAC
AAAGAAGCGTTGGCAAACGCCGCGACTACGCTACGCGACAACGGCGTGCGGGTGATTTATGACGTGGTTTTCAACCATAA
ACTTGGTGCCGACGAAAAAGAACAGGTTCACGTCTATAAGGCGGATGCCAACAACCGCAACGATATTAATGACCAGGGCT
TCGACGCCCTGGCCTACACCCGCTTCACTTTCCCCGGCAGACAAGGCACCTATTCCAAATTCATTTGGGATTACAAATGC
TTTGGCGGGGTTGATTACGTTGAGCAGCCCGATGAAAAAGGCGTGTTCAAAATCGCCAATGACTACGGCGACGACGGCTG
GAACGAGCAGGTCGGTGATGAGAAAGGCAACTACGATTATCTGATGGGGGCGGACGTCGAATTCCGCAACCCGGCGGTGG
TGGAAGAGCTGAAATACTGGGCGCGCTGGCTGCTGGAAACCCTGCCCTGCGATGGCTTCCGGTTGGATGCCGCCAAACAC
ATTCCGGCCTGGTTCTTTAAAGAGTGGGCTGACCACGTGCGTGACAGCGCACAGTGCGATCTGTTTATTGTCGCCGAATA
CTGGTCGCACGATTTGGCCGCGTTGCAACAGTATATTGACCTGGTCGAGGGCAAAGTGATGCTGTTCGATGTAGCCTTGC
ACCTGAAGTTTCATCAGGCATCGAAACAGGGAGACGGGTTCGACATGGCGCAGATTTTCACCGATACCCTGACCGCGGCC
GATCCGGCCCATTCGGTCACGCTGGTCGCCAACCACGACACGCAGCCGTTACAATCGCTGGAAGCACCGGTCGAACCCTG
GTTCAAACCGCTGGCCTACGCCCTTATCCTGTTGCGCGAACAAGGCGTGCCCTGCGTGTTCTATCCTGACCTGTACGGTG
CCAGCTATAAAGATAAAGGCAGCGACGGCCAGGAATACCAGATTGATATGCCGGTCATCCCTGAGCTGGAAAAGTTGATT
CAGGCGCGACAACGCTTTGCCAACGGCGTGCAAACCGATTATTTTGATGATAAAAACTGCGTGGCCTTCAGCCGCAGCGG
TACCGCCGACGCCCCTGGTTGCGTAGTGGTGCTCACCAACGGGGCTGAAAGTGGCAAAGCGGTTTCGCTGGGCACTGAAC
TGGCACATAAAAACTGGCGCGACCTGCTCGGTAACCGTCAGGATGAGGTCACCACCGACGAACAGGGGAATGCCACTTTC
CCGGTTAACGGCGGCAGCGTCAGCGTTTGGGTACTGGCAGAAACCCTGTAG

Upstream 100 bases:

>100_bases
AACCGAGTTTCCCTTTTGTCAAAACTAGCCCATGCTGTAACAGGATGGATGAGCCACGGCTCATTTCGCACCTCTTTCTG
GCAAACAGGACGGCTTGCTC

Downstream 100 bases:

>100_bases
CGGGTGCGCCGTTACGATCATGTGTCGCAGCGGCGGCAAACTAAATTTTGCCTTTCGGGTCTAAATTGAGGTAACACCTT
AGCAAACCATTCACACCGGA

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTKKTTLLQFFHWYYPDGGKLWQEAAERAAQIAQLGITDLWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSRATKYGD
KEALANAATTLRDNGVRVIYDVVFNHKLGADEKEQVHVYKADANNRNDINDQGFDALAYTRFTFPGRQGTYSKFIWDYKC
FGGVDYVEQPDEKGVFKIANDYGDDGWNEQVGDEKGNYDYLMGADVEFRNPAVVEELKYWARWLLETLPCDGFRLDAAKH
IPAWFFKEWADHVRDSAQCDLFIVAEYWSHDLAALQQYIDLVEGKVMLFDVALHLKFHQASKQGDGFDMAQIFTDTLTAA
DPAHSVTLVANHDTQPLQSLEAPVEPWFKPLAYALILLREQGVPCVFYPDLYGASYKDKGSDGQEYQIDMPVIPELEKLI
QARQRFANGVQTDYFDDKNCVAFSRSGTADAPGCVVVLTNGAESGKAVSLGTELAHKNWRDLLGNRQDEVTTDEQGNATF
PVNGGSVSVWVLAETL

Sequences:

>Translated_496_residues
MTKKTTLLQFFHWYYPDGGKLWQEAAERAAQIAQLGITDLWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSRATKYGD
KEALANAATTLRDNGVRVIYDVVFNHKLGADEKEQVHVYKADANNRNDINDQGFDALAYTRFTFPGRQGTYSKFIWDYKC
FGGVDYVEQPDEKGVFKIANDYGDDGWNEQVGDEKGNYDYLMGADVEFRNPAVVEELKYWARWLLETLPCDGFRLDAAKH
IPAWFFKEWADHVRDSAQCDLFIVAEYWSHDLAALQQYIDLVEGKVMLFDVALHLKFHQASKQGDGFDMAQIFTDTLTAA
DPAHSVTLVANHDTQPLQSLEAPVEPWFKPLAYALILLREQGVPCVFYPDLYGASYKDKGSDGQEYQIDMPVIPELEKLI
QARQRFANGVQTDYFDDKNCVAFSRSGTADAPGCVVVLTNGAESGKAVSLGTELAHKNWRDLLGNRQDEVTTDEQGNATF
PVNGGSVSVWVLAETL
>Mature_495_residues
TKKTTLLQFFHWYYPDGGKLWQEAAERAAQIAQLGITDLWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSRATKYGDK
EALANAATTLRDNGVRVIYDVVFNHKLGADEKEQVHVYKADANNRNDINDQGFDALAYTRFTFPGRQGTYSKFIWDYKCF
GGVDYVEQPDEKGVFKIANDYGDDGWNEQVGDEKGNYDYLMGADVEFRNPAVVEELKYWARWLLETLPCDGFRLDAAKHI
PAWFFKEWADHVRDSAQCDLFIVAEYWSHDLAALQQYIDLVEGKVMLFDVALHLKFHQASKQGDGFDMAQIFTDTLTAAD
PAHSVTLVANHDTQPLQSLEAPVEPWFKPLAYALILLREQGVPCVFYPDLYGASYKDKGSDGQEYQIDMPVIPELEKLIQ
ARQRFANGVQTDYFDDKNCVAFSRSGTADAPGCVVVLTNGAESGKAVSLGTELAHKNWRDLLGNRQDEVTTDEQGNATFP
VNGGSVSVWVLAETL

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=494, Percent_Identity=65.1821862348178, Blast_Score=690, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 55647; Mature: 55516

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKTTLLQFFHWYYPDGGKLWQEAAERAAQIAQLGITDLWLPPAYKGASGGYSVGYDSY
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCC
DLFDLGEFDQKGSRATKYGDKEALANAATTLRDNGVRVIYDVVFNHKLGADEKEQVHVYK
CCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCCEEEEEE
ADANNRNDINDQGFDALAYTRFTFPGRQGTYSKFIWDYKCFGGVDYVEQPDEKGVFKIAN
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHEEEEHHCCCCCHHCCCCCCCEEEEEC
DYGDDGWNEQVGDEKGNYDYLMGADVEFRNPAVVEELKYWARWLLETLPCDGFRLDAAKH
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHC
IPAWFFKEWADHVRDSAQCDLFIVAEYWSHDLAALQQYIDLVEGKVMLFDVALHLKFHQA
CHHHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHCCCEEEEEEEEHHEEHHC
SKQGDGFDMAQIFTDTLTAADPAHSVTLVANHDTQPLQSLEAPVEPWFKPLAYALILLRE
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHC
QGVPCVFYPDLYGASYKDKGSDGQEYQIDMPVIPELEKLIQARQRFANGVQTDYFDDKNC
CCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
VAFSRSGTADAPGCVVVLTNGAESGKAVSLGTELAHKNWRDLLGNRQDEVTTDEQGNATF
EEEECCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
PVNGGSVSVWVLAETL
EECCCCEEEEEEEECC
>Mature Secondary Structure 
TKKTTLLQFFHWYYPDGGKLWQEAAERAAQIAQLGITDLWLPPAYKGASGGYSVGYDSY
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCCCCCCCC
DLFDLGEFDQKGSRATKYGDKEALANAATTLRDNGVRVIYDVVFNHKLGADEKEQVHVYK
CCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHCCCCCCCCCCEEEEEE
ADANNRNDINDQGFDALAYTRFTFPGRQGTYSKFIWDYKCFGGVDYVEQPDEKGVFKIAN
CCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHEEEEHHCCCCCHHCCCCCCCEEEEEC
DYGDDGWNEQVGDEKGNYDYLMGADVEFRNPAVVEELKYWARWLLETLPCDGFRLDAAKH
CCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHC
IPAWFFKEWADHVRDSAQCDLFIVAEYWSHDLAALQQYIDLVEGKVMLFDVALHLKFHQA
CHHHHHHHHHHHHCCCCCCCEEEEEHHHCHHHHHHHHHHHHHCCCEEEEEEEEHHEEHHC
SKQGDGFDMAQIFTDTLTAADPAHSVTLVANHDTQPLQSLEAPVEPWFKPLAYALILLRE
CCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHC
QGVPCVFYPDLYGASYKDKGSDGQEYQIDMPVIPELEKLIQARQRFANGVQTDYFDDKNC
CCCCEEECCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
VAFSRSGTADAPGCVVVLTNGAESGKAVSLGTELAHKNWRDLLGNRQDEVTTDEQGNATF
EEEECCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
PVNGGSVSVWVLAETL
EECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400215; 9097040; 9278503; 1527488; 8371104 [H]