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The map label for this gene is gcvA [H]

Identifier: 157369613

GI number: 157369613

Start: 1499302

End: 1500231

Strand: Direct

Name: gcvA [H]

Synonym: Spro_1370

Alternate gene names: 157369613

Gene position: 1499302-1500231 (Clockwise)

Preceding gene: 157369610

Following gene: 157369614

Centisome position: 27.52

GC content: 60.97

Gene sequence:

>930_bases
ATGTCGTTATCCTTACCTTCGCTCGACGTGTTGAAAACCTTTGTGGTGGTGGCCCAGCGGCTTAATTTCACCCATGCCGC
CCAGAGTCTGCATCTGACGCAGGGCGCGGTCAGTCGGCAGATCCTCGGGCTGGAACAGCAACTGGGTTACCCGTTGTTCA
GCCGACGGGCGCGCGGCCTGACGCTGACCCCGCAGGGCGCGATGTTGCTGGCACCGGTGCAGCAGGCGCTCGGGCAGTTG
GATGAGGCACTGGCTCGGGTTGGGGCGCAACCGGGCACTCTGCGCATCAAGTGCCCGACCTGCGCCATGCGCTGGGTGCT
GCCGCGCATTATCCGGCTGCAAAATGAACGGCCGGAAATGCATATTGAGCTGACCGCCTCGGTGTCGCACGGGCTGGATT
TTGGCGCCGAACATTTTGATGCCGCGGTGGTATTCGGTCAGCCACAGGGCAAAAAATTGCGGGTGCATCACCTGTTCGAT
GAAATTCTTACGCCGGTCTGCACGCCGTCTTATCTGCCGGATTTGTCACGCCCCCAGACCCCGGCCGATCTGGCGGATAA
AACCCTGCTGCACCCCACGCGCGATCGCCGCGATTGGTTACTGTGGTTGAAGGCGGTGGGGCAGGAGGCGTTGCCATCTG
GCAAGGCGCAGCATTTCGATACGCTGGATCTGGCGATGAGTGCCGCGCTGCAAGGCTTTGGCATCGCCATCGGTGACCTG
TGTCTGCTGGAGGAAGATATCCAGGCGCAGCGTATCGTCACGCCGTTCCCGCTCAGCGTGGCCAGCGGCGCAGCCTATTA
TTTGGTCTATCCTGAACGGGCGGTGGTGCCGCAGGCATTAACCGAACTGGTCAATTTCCTCAGCGATGAAGCGGCGCAAA
GCCGTAGCCGGCTGCAAAATTATCGAGCAGCCTCCGCTAACAGCTTGTAA

Upstream 100 bases:

>100_bases
TCGCCACGGGCGCTGCATGCAGCGCCCCTACCATGCGGGATCGCCGTAGGGGACGCATATATTCGTCCCAACCATTTTCT
GGCATCTAAAGGAGCTAACT

Downstream 100 bases:

>100_bases
CTTTTAACATTACAAATTATTTCTGCGCCGAAAAGGAGTATGGCACACTGCCTTTTCACATTGATAAATCATCTCCGTCG
CCGCCTGCGGCGGGAACATG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSLSLPSLDVLKTFVVVAQRLNFTHAAQSLHLTQGAVSRQILGLEQQLGYPLFSRRARGLTLTPQGAMLLAPVQQALGQL
DEALARVGAQPGTLRIKCPTCAMRWVLPRIIRLQNERPEMHIELTASVSHGLDFGAEHFDAAVVFGQPQGKKLRVHHLFD
EILTPVCTPSYLPDLSRPQTPADLADKTLLHPTRDRRDWLLWLKAVGQEALPSGKAQHFDTLDLAMSAALQGFGIAIGDL
CLLEEDIQAQRIVTPFPLSVASGAAYYLVYPERAVVPQALTELVNFLSDEAAQSRSRLQNYRAASANSL

Sequences:

>Translated_309_residues
MSLSLPSLDVLKTFVVVAQRLNFTHAAQSLHLTQGAVSRQILGLEQQLGYPLFSRRARGLTLTPQGAMLLAPVQQALGQL
DEALARVGAQPGTLRIKCPTCAMRWVLPRIIRLQNERPEMHIELTASVSHGLDFGAEHFDAAVVFGQPQGKKLRVHHLFD
EILTPVCTPSYLPDLSRPQTPADLADKTLLHPTRDRRDWLLWLKAVGQEALPSGKAQHFDTLDLAMSAALQGFGIAIGDL
CLLEEDIQAQRIVTPFPLSVASGAAYYLVYPERAVVPQALTELVNFLSDEAAQSRSRLQNYRAASANSL
>Mature_308_residues
SLSLPSLDVLKTFVVVAQRLNFTHAAQSLHLTQGAVSRQILGLEQQLGYPLFSRRARGLTLTPQGAMLLAPVQQALGQLD
EALARVGAQPGTLRIKCPTCAMRWVLPRIIRLQNERPEMHIELTASVSHGLDFGAEHFDAAVVFGQPQGKKLRVHHLFDE
ILTPVCTPSYLPDLSRPQTPADLADKTLLHPTRDRRDWLLWLKAVGQEALPSGKAQHFDTLDLAMSAALQGFGIAIGDLC
LLEEDIQAQRIVTPFPLSVASGAAYYLVYPERAVVPQALTELVNFLSDEAAQSRSRLQNYRAASANSL

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=272, Percent_Identity=35.2941176470588, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=29.9319727891156, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1788706, Length=275, Percent_Identity=28.3636363636364, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=28.3276450511945, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=26.3698630136986, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790262, Length=232, Percent_Identity=29.7413793103448, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=25.8389261744966, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789639, Length=289, Percent_Identity=24.2214532871972, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787128, Length=282, Percent_Identity=24.822695035461, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787879, Length=119, Percent_Identity=35.2941176470588, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33924; Mature: 33792

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSLPSLDVLKTFVVVAQRLNFTHAAQSLHLTQGAVSRQILGLEQQLGYPLFSRRARGL
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
TLTPQGAMLLAPVQQALGQLDEALARVGAQPGTLRIKCPTCAMRWVLPRIIRLQNERPEM
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCE
HIELTASVSHGLDFGAEHFDAAVVFGQPQGKKLRVHHLFDEILTPVCTPSYLPDLSRPQT
EEEEEEEHHCCCCCCHHHHCEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
PADLADKTLLHPTRDRRDWLLWLKAVGQEALPSGKAQHFDTLDLAMSAALQGFGIAIGDL
CHHHHHHHHCCCCCCHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
CLLEEDIQAQRIVTPFPLSVASGAAYYLVYPERAVVPQALTELVNFLSDEAAQSRSRLQN
HHHHHHHHHHHCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAASANSL
HHCCCCCCC
>Mature Secondary Structure 
SLSLPSLDVLKTFVVVAQRLNFTHAAQSLHLTQGAVSRQILGLEQQLGYPLFSRRARGL
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
TLTPQGAMLLAPVQQALGQLDEALARVGAQPGTLRIKCPTCAMRWVLPRIIRLQNERPEM
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCE
HIELTASVSHGLDFGAEHFDAAVVFGQPQGKKLRVHHLFDEILTPVCTPSYLPDLSRPQT
EEEEEEEHHCCCCCCHHHHCEEEEEECCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
PADLADKTLLHPTRDRRDWLLWLKAVGQEALPSGKAQHFDTLDLAMSAALQGFGIAIGDL
CHHHHHHHHCCCCCCHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHH
CLLEEDIQAQRIVTPFPLSVASGAAYYLVYPERAVVPQALTELVNFLSDEAAQSRSRLQN
HHHHHHHHHHHCCCCCCCHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAASANSL
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]