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The map label for this gene is ddlA [H]

Identifier: 157369610

GI number: 157369610

Start: 1495833

End: 1496936

Strand: Direct

Name: ddlA [H]

Synonym: Spro_1367

Alternate gene names: 157369610

Gene position: 1495833-1496936 (Clockwise)

Preceding gene: 157369606

Following gene: 157369613

Centisome position: 27.45

GC content: 56.52

Gene sequence:

>1104_bases
GTGAGCAAATTACGTGTTGGGGTGATCTTTGGCGGTAAATCGGCGGAGCATGAAGTTTCGCTGCAGTCGGCAAAAAACAT
TGTGGATGCAATCGACAAAGAGAAGTTTGACGTCACGCTGCTGGGGATCGACAAGCAGGGGCAATGGCACATTAACGATG
CGTCCAATTACCTGCTGAATGCGGAAAACCCGGCGCTGATCGCGCTGAACCGCTCAAATAAAAATGTGGCGCTGATCCCC
GGTCAGGAGAAACAGCAGCTGATCGAGTCGGGCAGCCAGGGCGCACTGGGCCAGTTGGATGTGATATTCCCGATCGTACA
CGGCACATTGGGTGAGGACGGTTCGCTGCAGGGGCTGTTGCGAATGGCTAATCTGCCGTTCGTCGGTGCCGGCGTGTTGG
GTTCGGCGGTCAGCATGGATAAAGACGTGACCAAACGCCTGCTGCGTGATGCCGGGCTGGCGATAGCGCCGTTTGTGACG
CTGACCCGCAGCAATCGCGCCAAATTCGATTTTGAGCAGCTTAGCGAACGTCTGGGGCTGCCGCTGTTTGTTAAACCGGC
CAACCAGGGCTCTTCGGTAGGCGTCAGCAAGGTACAGGATCGCGCCGGTTTTGAGGCTGCGGTGGCGCTGGCGTTCAGCT
TCGATCATAAGGTGCTGGTGGAGTCGGCGATTGTCGGTCGTGAAATAGAATGCGCGGTATTGGGCAACGACGAACCTCAG
GCCAGCCTGTGCGGCGAAATCGTGTTGAGCGACGCCTTTTATTCCTACGACACCAAATACATCAATGAGCAGGGTGCTCA
GGTGATAGTGCCGGCGGCGATTGAGCCGGAGGTGAGCGATAAAATCCGCGACGTGGCGCTAAAAGCCTTTCGGGCGCTGG
AGTGTTTCGGGCTGGCGCGCGTCGATGTATTTCTGACGCCGGACAACAACGTGGTGATCAACGAAATCAACACGCTGCCA
GGCTTTACCAATATCAGCATGTATCCCAAACTGTGGGCCGCCAGCGGTGTCAGCTACAGCCAATTGATCACTCGCCTGAT
TGAGCTGGCGATGGAACGCCATCAGCAGGATCGCGCTTTGAACAGCTCGGTGTTTGACCGGTAA

Upstream 100 bases:

>100_bases
GCATGGGAATATTCTTGGTCACAATGATTGTCGCCGCCCGGCAATGCGTTTATTGTGATAAACGACTTTTTTGCACTGAT
TTTTCAGAAGGATTTAGGGC

Downstream 100 bases:

>100_bases
GCGGGCATAAAAAAAGAACCAGGTTGGTGGCCTGGTTCTTCTATTATCGGCTCTAAAAGAGAGCTGTGTCGCACATACTT
GTCATCCTTTTTTCAGTGTA

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetase A [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSKLRVGVIFGGKSAEHEVSLQSAKNIVDAIDKEKFDVTLLGIDKQGQWHINDASNYLLNAENPALIALNRSNKNVALIP
GQEKQQLIESGSQGALGQLDVIFPIVHGTLGEDGSLQGLLRMANLPFVGAGVLGSAVSMDKDVTKRLLRDAGLAIAPFVT
LTRSNRAKFDFEQLSERLGLPLFVKPANQGSSVGVSKVQDRAGFEAAVALAFSFDHKVLVESAIVGREIECAVLGNDEPQ
ASLCGEIVLSDAFYSYDTKYINEQGAQVIVPAAIEPEVSDKIRDVALKAFRALECFGLARVDVFLTPDNNVVINEINTLP
GFTNISMYPKLWAASGVSYSQLITRLIELAMERHQQDRALNSSVFDR

Sequences:

>Translated_367_residues
MSKLRVGVIFGGKSAEHEVSLQSAKNIVDAIDKEKFDVTLLGIDKQGQWHINDASNYLLNAENPALIALNRSNKNVALIP
GQEKQQLIESGSQGALGQLDVIFPIVHGTLGEDGSLQGLLRMANLPFVGAGVLGSAVSMDKDVTKRLLRDAGLAIAPFVT
LTRSNRAKFDFEQLSERLGLPLFVKPANQGSSVGVSKVQDRAGFEAAVALAFSFDHKVLVESAIVGREIECAVLGNDEPQ
ASLCGEIVLSDAFYSYDTKYINEQGAQVIVPAAIEPEVSDKIRDVALKAFRALECFGLARVDVFLTPDNNVVINEINTLP
GFTNISMYPKLWAASGVSYSQLITRLIELAMERHQQDRALNSSVFDR
>Mature_366_residues
SKLRVGVIFGGKSAEHEVSLQSAKNIVDAIDKEKFDVTLLGIDKQGQWHINDASNYLLNAENPALIALNRSNKNVALIPG
QEKQQLIESGSQGALGQLDVIFPIVHGTLGEDGSLQGLLRMANLPFVGAGVLGSAVSMDKDVTKRLLRDAGLAIAPFVTL
TRSNRAKFDFEQLSERLGLPLFVKPANQGSSVGVSKVQDRAGFEAAVALAFSFDHKVLVESAIVGREIECAVLGNDEPQA
SLCGEIVLSDAFYSYDTKYINEQGAQVIVPAAIEPEVSDKIRDVALKAFRALECFGLARVDVFLTPDNNVVINEINTLPG
FTNISMYPKLWAASGVSYSQLITRLIELAMERHQQDRALNSSVFDR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=364, Percent_Identity=71.1538461538462, Blast_Score=546, Evalue=1e-157,
Organism=Escherichia coli, GI1786280, Length=252, Percent_Identity=42.0634920634921, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 39785; Mature: 39654

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLRVGVIFGGKSAEHEVSLQSAKNIVDAIDKEKFDVTLLGIDKQGQWHINDASNYLLN
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCEEEE
AENPALIALNRSNKNVALIPGQEKQQLIESGSQGALGQLDVIFPIVHGTLGEDGSLQGLL
CCCCEEEEEECCCCEEEEECCCHHHHHHHCCCCCCCCCHHEEEEEHHCCCCCCCCHHHHH
RMANLPFVGAGVLGSAVSMDKDVTKRLLRDAGLAIAPFVTLTRSNRAKFDFEQLSERLGL
HHHCCCEEECCHHHHHHHCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSKVQDRAGFEAAVALAFSFDHKVLVESAIVGREIECAVLGNDEPQ
CEEEEECCCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHCCCEEEEEEECCCCCH
ASLCGEIVLSDAFYSYDTKYINEQGAQVIVPAAIEPEVSDKIRDVALKAFRALECFGLAR
HHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEE
VDVFLTPDNNVVINEINTLPGFTNISMYPKLWAASGVSYSQLITRLIELAMERHQQDRAL
EEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
NSSVFDR
HHHHCCC
>Mature Secondary Structure 
SKLRVGVIFGGKSAEHEVSLQSAKNIVDAIDKEKFDVTLLGIDKQGQWHINDASNYLLN
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEECCCCEEEE
AENPALIALNRSNKNVALIPGQEKQQLIESGSQGALGQLDVIFPIVHGTLGEDGSLQGLL
CCCCEEEEEECCCCEEEEECCCHHHHHHHCCCCCCCCCHHEEEEEHHCCCCCCCCHHHHH
RMANLPFVGAGVLGSAVSMDKDVTKRLLRDAGLAIAPFVTLTRSNRAKFDFEQLSERLGL
HHHCCCEEECCHHHHHHHCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCC
PLFVKPANQGSSVGVSKVQDRAGFEAAVALAFSFDHKVLVESAIVGREIECAVLGNDEPQ
CEEEEECCCCCCCCHHHHHHHCCCCEEEEEEEECCCHHHHHHHHCCCEEEEEEECCCCCH
ASLCGEIVLSDAFYSYDTKYINEQGAQVIVPAAIEPEVSDKIRDVALKAFRALECFGLAR
HHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCEEE
VDVFLTPDNNVVINEINTLPGFTNISMYPKLWAASGVSYSQLITRLIELAMERHQQDRAL
EEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
NSSVFDR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]