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The map label for this gene is gcvA [H]

Identifier: 157369584

GI number: 157369584

Start: 1471577

End: 1472476

Strand: Reverse

Name: gcvA [H]

Synonym: Spro_1341

Alternate gene names: 157369584

Gene position: 1472476-1471577 (Counterclockwise)

Preceding gene: 157369586

Following gene: 157369582

Centisome position: 27.02

GC content: 54.33

Gene sequence:

>900_bases
ATGAAATCTTCCCGTTTACCGCCGCTGGGTGCACTGCGCGCCTTCCATGCGGTTGCCGATTGCCTTAGCTTCAAGCAGGC
TGCCGGGCAACTGAATGTCAGCGCCACCGCTGTCAGCCATCAAATCCGGTTGATTGAATCCATGCTGGAATGCCGCGTGT
TCGAACGCAACGCGCAGGGGGTGAAACTGACCGAGGCCGGACACTTGCTGTATACGGGCACGCAATCAGCCTTTACCGCC
CTGGAAGAGTCGGTAAAAAAAATTGATCAGGCCAGACAACCTCCGGCGCTGACCATCACCACCACCTCGAACTTTTTGAC
CAATTGGCTGGTGCTACGCCTTGAAAGCTTTAAGGCGCAATTTCCCGAGATTGATTTACACCTGCATACCAGCGTGCAAA
GCGTCGACTTAACGCAGCGAACCGTCGACGTAGCCATACGTTATCGTGAAACCGCTGAAATAGGCCTTCACAGTACCTTG
CTGTATGAGGATCGGTTTATTGTGGTTGCCAGCCCGGCACTGGCCTTGAATACCCTGAGCGACCTGCACCGCACGACGCT
GTTTCACGTCGAGAACAGGCATGTTCCTGCCGAAAACCCTGACTGGAAACACTGGCAGCAAGGTTACGGGCCGGAGAACC
TGAATATTTCAAGAGGCCTGTATTTCAGCGATGAAACCCATGCGTTGCAGGCCGCCGTGGCAGGGCAAGGGGTGGTTATT
GCCAGTAAGTTGCTGGCAGAGGATTTAATAAAACGCAAAATGCTGGTGGCACCGTTCGATCTGTCTTTACCCGGCGCGAA
TTATTATCTGGTGACGCTGGAAGACACTGCCGATCGCCCCGACGTCGTGTCATTACGCAACTGGATGCAGAAAAATATGC
ACCCCGGCCCGCTGGAGTAA

Upstream 100 bases:

>100_bases
AAATTTATTTCATCCTTACAAGGAGGTATGTTGAGCCTATCGCATATATAATCAGGCAATCAAAGTCAGAGTTTTCACCC
ATGGATGAAAATAACTCATC

Downstream 100 bases:

>100_bases
GGACTTATTGACTGAGCCTGGAGCTTAATGCCAATGCAGCACCACGGCACCGGCCAATACCGTTAACACGCCCAGCCACT
GTAGGGCGGCAAGGCGCTCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKSSRLPPLGALRAFHAVADCLSFKQAAGQLNVSATAVSHQIRLIESMLECRVFERNAQGVKLTEAGHLLYTGTQSAFTA
LEESVKKIDQARQPPALTITTTSNFLTNWLVLRLESFKAQFPEIDLHLHTSVQSVDLTQRTVDVAIRYRETAEIGLHSTL
LYEDRFIVVASPALALNTLSDLHRTTLFHVENRHVPAENPDWKHWQQGYGPENLNISRGLYFSDETHALQAAVAGQGVVI
ASKLLAEDLIKRKMLVAPFDLSLPGANYYLVTLEDTADRPDVVSLRNWMQKNMHPGPLE

Sequences:

>Translated_299_residues
MKSSRLPPLGALRAFHAVADCLSFKQAAGQLNVSATAVSHQIRLIESMLECRVFERNAQGVKLTEAGHLLYTGTQSAFTA
LEESVKKIDQARQPPALTITTTSNFLTNWLVLRLESFKAQFPEIDLHLHTSVQSVDLTQRTVDVAIRYRETAEIGLHSTL
LYEDRFIVVASPALALNTLSDLHRTTLFHVENRHVPAENPDWKHWQQGYGPENLNISRGLYFSDETHALQAAVAGQGVVI
ASKLLAEDLIKRKMLVAPFDLSLPGANYYLVTLEDTADRPDVVSLRNWMQKNMHPGPLE
>Mature_299_residues
MKSSRLPPLGALRAFHAVADCLSFKQAAGQLNVSATAVSHQIRLIESMLECRVFERNAQGVKLTEAGHLLYTGTQSAFTA
LEESVKKIDQARQPPALTITTTSNFLTNWLVLRLESFKAQFPEIDLHLHTSVQSVDLTQRTVDVAIRYRETAEIGLHSTL
LYEDRFIVVASPALALNTLSDLHRTTLFHVENRHVPAENPDWKHWQQGYGPENLNISRGLYFSDETHALQAAVAGQGVVI
ASKLLAEDLIKRKMLVAPFDLSLPGANYYLVTLEDTADRPDVVSLRNWMQKNMHPGPLE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=35.4948805460751, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786448, Length=256, Percent_Identity=33.203125, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=27.7397260273973, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1787128, Length=234, Percent_Identity=27.3504273504274, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786401, Length=163, Percent_Identity=28.2208588957055, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87081978, Length=243, Percent_Identity=25.1028806584362, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789440, Length=232, Percent_Identity=23.7068965517241, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788297, Length=167, Percent_Identity=29.940119760479, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33374; Mature: 33374

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSRLPPLGALRAFHAVADCLSFKQAAGQLNVSATAVSHQIRLIESMLECRVFERNAQG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCC
VKLTEAGHLLYTGTQSAFTALEESVKKIDQARQPPALTITTTSNFLTNWLVLRLESFKAQ
EEEECCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCC
FPEIDLHLHTSVQSVDLTQRTVDVAIRYRETAEIGLHSTLLYEDRFIVVASPALALNTLS
CCEEEEEEECCEEHHHHHHHEEEEHEEEHHHHHHCCCEEEEEECEEEEEECCHHHHHHHH
DLHRTTLFHVENRHVPAENPDWKHWQQGYGPENLNISRGLYFSDETHALQAAVAGQGVVI
HHHHHHEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHCCCCHHH
ASKLLAEDLIKRKMLVAPFDLSLPGANYYLVTLEDTADRPDVVSLRNWMQKNMHPGPLE
HHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSSRLPPLGALRAFHAVADCLSFKQAAGQLNVSATAVSHQIRLIESMLECRVFERNAQG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCC
VKLTEAGHLLYTGTQSAFTALEESVKKIDQARQPPALTITTTSNFLTNWLVLRLESFKAQ
EEEECCCEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHCC
FPEIDLHLHTSVQSVDLTQRTVDVAIRYRETAEIGLHSTLLYEDRFIVVASPALALNTLS
CCEEEEEEECCEEHHHHHHHEEEEHEEEHHHHHHCCCEEEEEECEEEEEECCHHHHHHHH
DLHRTTLFHVENRHVPAENPDWKHWQQGYGPENLNISRGLYFSDETHALQAAVAGQGVVI
HHHHHHEEEEECCCCCCCCCCHHHHHHCCCCCCCCCCCCEEECCCHHHHHHHHCCCCHHH
ASKLLAEDLIKRKMLVAPFDLSLPGANYYLVTLEDTADRPDVVSLRNWMQKNMHPGPLE
HHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]