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The map label for this gene is moaE [H]

Identifier: 157369567

GI number: 157369567

Start: 1453384

End: 1453854

Strand: Direct

Name: moaE [H]

Synonym: Spro_1324

Alternate gene names: 157369567

Gene position: 1453384-1453854 (Clockwise)

Preceding gene: 157369566

Following gene: 157369568

Centisome position: 26.67

GC content: 60.51

Gene sequence:

>471_bases
ATGGTGGAAAATACCCGTATCCGCGTGGGGGAAGCGCCGTTCAACGTCGGCGAAGAATACCAGTGGCTGGCGCAGTGCGA
CGATGACGGTGCCGTGGTGACCTTTACCGGCAAGGTACGTAACCATAATCTGGGCGATAACGTCAGTGCGCTGACGCTGG
AACACTACCCGGGCATGACCGAAAAAGCGCTGGCGGAAATCGTGGACGAAGCACGCAGCCGCTGGCCATTGCAGCGCGCG
ACGGTGATCCACCGCGTGGGCGAGCTGTTTCCTGGCGATGAGATTGTGTTTGTTGGCGTGACCAGCGCGCACCGCAGCAT
GGCATTCGAAGCCAGCGAGTTCATCATGGACTACCTGAAAACCCGCGCCCCGTTCTGGAAACGCGAAGCCGTTGGCCAGG
GCGACCGCTGGGTTGACGCGCGCGACAGCGATCGTCAGGCGGCGGAACGCTGGCATAAAATCATTAAGTGA

Upstream 100 bases:

>100_bases
GCAAGTTGCTGACGGCGGTAAACCAATCCCTGGTGGCGGCGGAACATCCGCTGCGTGCCGGTGATGAAGTCGCTTTCTTT
CCACCGGTAACCGGGGGTTA

Downstream 100 bases:

>100_bases
TCTGTACACCCTATGAATTTCAAGCTGTAGCTAGGCGGCCAGCTCACTCATTCCCAGGCGCTTACTTGAGTAAGTAACTG
GGGTGAGTGAGTGCAGGTAA

Product: molybdopterin guanine dinucleotide biosynthesis protein MoaE

Products: NA

Alternate protein names: MPT synthase subunit 2; Molybdenum cofactor biosynthesis protein E; Molybdopterin-converting factor large subunit; Molybdopterin-converting factor subunit 2 [H]

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MVENTRIRVGEAPFNVGEEYQWLAQCDDDGAVVTFTGKVRNHNLGDNVSALTLEHYPGMTEKALAEIVDEARSRWPLQRA
TVIHRVGELFPGDEIVFVGVTSAHRSMAFEASEFIMDYLKTRAPFWKREAVGQGDRWVDARDSDRQAAERWHKIIK

Sequences:

>Translated_156_residues
MVENTRIRVGEAPFNVGEEYQWLAQCDDDGAVVTFTGKVRNHNLGDNVSALTLEHYPGMTEKALAEIVDEARSRWPLQRA
TVIHRVGELFPGDEIVFVGVTSAHRSMAFEASEFIMDYLKTRAPFWKREAVGQGDRWVDARDSDRQAAERWHKIIK
>Mature_156_residues
MVENTRIRVGEAPFNVGEEYQWLAQCDDDGAVVTFTGKVRNHNLGDNVSALTLEHYPGMTEKALAEIVDEARSRWPLQRA
TVIHRVGELFPGDEIVFVGVTSAHRSMAFEASEFIMDYLKTRAPFWKREAVGQGDRWVDARDSDRQAAERWHKIIK

Specific function: Converts molybdopterin precursor Z into molybdopterin. This requires the incorporation of two sulfur atoms into precursor Z to generate a dithiolene group. The sulfur is provided by moaD [H]

COG id: COG0314

COG function: function code H; Molybdopterin converting factor, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moaE family [H]

Homologues:

Organism=Homo sapiens, GI4758732, Length=108, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=4e-16,
Organism=Escherichia coli, GI1787003, Length=147, Percent_Identity=82.312925170068, Blast_Score=255, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17509279, Length=107, Percent_Identity=33.6448598130841, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356259, Length=143, Percent_Identity=32.1678321678322, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003448 [H]

Pfam domain/function: PF02391 MoaE [H]

EC number: NA

Molecular weight: Translated: 17789; Mature: 17789

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVENTRIRVGEAPFNVGEEYQWLAQCDDDGAVVTFTGKVRNHNLGDNVSALTLEHYPGMT
CCCCCEEEECCCCCCCCCHHHHHEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCC
EKALAEIVDEARSRWPLQRATVIHRVGELFPGDEIVFVGVTSAHRSMAFEASEFIMDYLK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
TRAPFWKREAVGQGDRWVDARDSDRQAAERWHKIIK
HCCCCHHHHHCCCCCCEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVENTRIRVGEAPFNVGEEYQWLAQCDDDGAVVTFTGKVRNHNLGDNVSALTLEHYPGMT
CCCCCEEEECCCCCCCCCHHHHHEEECCCCEEEEEECCCCCCCCCCCCEEEEECCCCCCC
EKALAEIVDEARSRWPLQRATVIHRVGELFPGDEIVFVGVTSAHRSMAFEASEFIMDYLK
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
TRAPFWKREAVGQGDRWVDARDSDRQAAERWHKIIK
HCCCCHHHHHCCCCCCEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]